BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0007.p (1046 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance ... 649 0.0 gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump pr... 234 2e-62 gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump pr... 225 1e-59 gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump tr... 217 3e-57 gi|300313244|ref|YP_003777336.1| multidrug efflux system transme... 213 9e-56 >gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1073 Score = 649 bits (1675), Expect = 0.0, Method: Compositional matrix adjust. Identities = 382/1062 (35%), Positives = 612/1062 (57%), Gaps = 31/1062 (2%) Query: 1 MIEKLIKWSLRSRFLVLFGVLFLGGWGLWALKTTPVDALPDLSDVQVIIRTTYAGQAPQI 60 M E++I++++ R+LVL VL +G +G+++ + P+DA+PD+++VQV I T+ G +P Sbjct: 1 MFERIIRFAIEHRWLVLLAVLGMGAYGVYSYQKLPIDAVPDITNVQVQINTSAPGYSPLE 60 Query: 61 VENQVTYPLATTMLSVPGAKTVRGYSFFGDSFVYVLFEDGTDMYWARSRVLEYLNQVQGR 120 E +++YP+ T M +P + VR S +G S V V+F+DGTD+Y+AR V E + + + Sbjct: 61 AEQRISYPIETLMSGLPNLEQVRSLSRYGLSQVTVIFKDGTDIYFARQLVNERIQLARSQ 120 Query: 121 LPPSAKASLGPDATGVGWIYEYALVDRTGQ-------HDLAQLRSLQDWFLKYELKSLPN 173 LPP +GP +TG+G IY + + R + LR +QDW +K +L+ +P Sbjct: 121 LPPGIDPGMGPISTGLGEIYLWTVESRDDARKPDGTPYTATDLREIQDWVIKPQLRLVPG 180 Query: 174 VAEVATVGGMVKQYQVVLDPVKLAAYNIAQDKVVDAIKKANQETGGSVLEMAETEYMVRA 233 V E+ T+GG K+Y V KLA+Y + D ++ A++K N G +E +Y++RA Sbjct: 181 VTEINTIGGFAKEYLVAPSAEKLASYGMTMDTIIKALEKNNSNVGAGYIERRGEQYLIRA 240 Query: 234 SGYLKTLADFRNIPLATGRNGVPVKLGDVAIIQFGPEMRRGIAELNGEGESVGGVIILRS 293 G + ++ D NI LA + GVP+++ DVA ++ G E+R G A NG E V G + + Sbjct: 241 PGQVASIDDIGNIVLANVK-GVPIRIRDVAEVEIGRELRTGAATENGR-EVVLGTVFMLI 298 Query: 294 GKNARETIVAVKSRLEELKKSLPAGVEVVPVYDRSQLIDRAIDNLSHKLLEEFIVVAVVC 353 G+N+R+ AV ++EE+ +SLP GVE V VYDR+ L+D+AI + L+E ++V V Sbjct: 299 GENSRKVSQAVARKMEEINRSLPKGVEAVTVYDRTVLVDKAIATVKKNLVEGALLVVAVL 358 Query: 354 GLFLWHLRSALVAIVSLPLGIMIAFIVMRYQGVNANIMSLGGIAIAIGAMVDAAVVMIEN 413 +FL ++R+A++ + +PL ++ F M V+AN++SLG A+ G ++D AVV++EN Sbjct: 359 FVFLGNIRAAIITAMVIPLAMLFTFTGMVSNKVSANLLSLG--ALDFGIIIDGAVVIVEN 416 Query: 414 AHKHIEEWKHRHPGQPLQGGNHWRVIGEAAAEVGPTLFFCLLIITLSFIPVFTLEAQEGR 473 + + + H G+ L + + AA E L F LII + ++P+F L EG+ Sbjct: 417 CVRRLAHAQAHH-GRTLTRSERFHEVFAAAKEARRPLIFGQLIIMIVYLPMFALTGVEGK 475 Query: 474 LFGPLAFTKTYAMAGAAGLSVTLIPILMGYMIRGKIPSENSNPLNRWLIRCYRPLLEAVL 533 +F P+AFT A+ GA LSVT IP + I K+ +E N L W R Y P+L+ ++ Sbjct: 476 MFTPMAFTVVAALIGAMILSVTFIPAAIALFIGEKV-AEKENILMLWAKRLYEPMLKTIM 534 Query: 534 RRPRTTLAIAALALLTTLWPISRLGGEFLPQMDEEDLLYMPSALPGLSASKAAELLQQTD 593 L A +L SRLG EF+P ++E D+ +PG S S++ E+ +Q + Sbjct: 535 ENKPVVLTFAGALILVCGVVASRLGSEFVPSLNEGDMAIQALRIPGTSLSQSLEMQKQIE 594 Query: 594 RLIK-TVPEVASVFGKAGRAESATDPAPLEMFETTIQFKPRDQW-RPGMTPTKLVNEL-D 650 + +K PEV VF + G AE A+DP P + + I KP++QW P + +L+ + D Sbjct: 595 KTLKQKFPEVERVFARTGTAEVASDPMPPNISDGYIMLKPQEQWPEPKKSREELLEAIQD 654 Query: 651 RVVKVPGLSNIWVPPIRNRLDMLATGIKSPIGVKVSGTSLADIDKTAQQIEQVAKEVPGV 710 V K+PG S + PI+ R + L +G++S + VKV G + ++ TA +I V +++PG Sbjct: 655 EVAKIPGNSYEFSQPIQLRFNELISGVRSDVAVKVFGDDMKVLNDTASKISAVLEKIPGA 714 Query: 711 SSALAERLSGGRYVDVDIDRLTAANYGLNIADVQSIVSSAIGGENVGETIEGLARYPISV 770 + E+ +G + V+IDR A YGLN++D+Q V++AIGG+ G +G R+ I V Sbjct: 715 TEVKIEQTTGLPMLTVEIDREKTARYGLNVSDIQDTVATAIGGKEAGTMFQGDRRFGIVV 774 Query: 771 RYPRETRDSLERLRDLPILTVSGQQIT------LGTVAKLKISDGPPMLKSENARPSGWI 824 R P + R LE +R LPI + T LG VA L I+ GP + E+ + + Sbjct: 775 RLPDQARTDLEAIRRLPIALPPQEGATRVSYVPLGEVASLTIAPGPNQISREDGKRRIVV 834 Query: 825 YVDVRGRDLSSVVADLRAAVSSKVKLTPGLSIAYSGQFEYLERANARLKLVVPATLMIIF 884 +VRGRDL VA+ + S VK+ G + GQFE L+ A RL++V+P L+++F Sbjct: 835 SANVRGRDLGGFVAEAERQLQSAVKVPAGYWTTWGGQFENLQSATQRLQIVIPVALLMVF 894 Query: 885 VLLYLTFRRFDEAALIMASLPFALVGGIWFLYAQGYNLSVATGVGFIALAGVSAEFGVVM 944 VLL+ F + L+ +PFAL GGI L +G +S++ VGFIAL+GV+ G+VM Sbjct: 895 VLLFAMFSNVKDGLLVFTGIPFALTGGILALAMRGIPMSISAAVGFIALSGVAVLNGLVM 954 Query: 945 LLYLKQAVSAREQQGGTISVPVLEDAIREGAVLRVRPKAMTVAVILAGLIPILWGSGAGS 1004 + +++ S RE +G T L+ AI EGA+ R+RP MT V G +P+ +G G+ Sbjct: 955 ISFIR---SLRE-EGRT-----LDAAIHEGAMTRLRPVLMTALVASLGFVPMALATGTGA 1005 Query: 1005 EVMSRIAAPMVGGMITAPLLSMFVIPAAYRLMRGRQPKANPA 1046 EV +A ++GG++++ L++ V+P Y+L + + NPA Sbjct: 1006 EVQRPLATVVIGGILSSTALTLLVLPLLYKLAHDKDEEDNPA 1047 >gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1063 Score = 234 bits (598), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 264/1040 (25%), Positives = 482/1040 (46%), Gaps = 68/1040 (6%) Query: 31 LKTTPVDALPDLSDVQVIIRTTYAGQAPQIVENQVTYPLATTMLSV-PGAKTVRGYSFFG 89 LK D P+++ V I Y G + Q + ++ + +V + + S G Sbjct: 29 LKNMATDIFPEINIPVVSIIWNYNGLSAQEMGQRIAAQNERGLTTVVSDIEHIESQSLAG 88 Query: 90 DSFVYVLFEDGTDMYWARSRVLEYLNQVQGRLPPSAKASLGPDATGVGW-IYEYALVDRT 148 S + V F+ ++ A ++V+ + +LPP L + + + AL T Sbjct: 89 VSVIKVFFQPKANIQTAIAQVVASVQTQVRQLPPGITPPLVIKYSASSIPVMQLALSSPT 148 Query: 149 GQHDLAQLRSLQDWFLKYELKSLPNVAEVATVGGMVKQYQVVLDPVKLAAYNIAQDKVVD 208 ++Q L+ +L ++P VA GG + V LD L A +A VV+ Sbjct: 149 LPEQTVFDAAVQT--LRPQLVTIPGVAVPYPYGGKSRLISVDLDTQALQARGLAPIDVVN 206 Query: 209 AIKKANQETGGSVLEMAETEYMVRASGYLKTLADFRNIPLATGRNGVPVKLGDVAIIQFG 268 AI N + TEY V+ +G + +A +P+ T NG + L DVA ++ G Sbjct: 207 AINTQNLILPSGTAKFGATEYSVKINGSPEAVAGLNELPVRT-TNGATIYLKDVANVRDG 265 Query: 269 PEMRRGIAELNGEGESVGGVIILRSGKNARETIVAVKSRLEELKKSLPAGVEVVPVYDRS 328 + + +G V I+ G + + + ++ L ++LP +++ P++D+S Sbjct: 266 FSPQTNVVRQDG-ARGVLLSILKNGGASTLDIVDNLRKLLPTAAQTLPEDIKITPLFDQS 324 Query: 329 QLIDRAIDN-LSHKLLEEFIVVAVVCGLFLWHLRSALVAIVSLPLGIMIAFIVMRYQGVN 387 + A+ +S L+ + A+V LFL + RS L+ +++PL IM A +++ G Sbjct: 325 VFVKAAVTGVISEALIAAGLTAAMVL-LFLGNWRSTLIIGLTIPLSIMAAILILAALGET 383 Query: 388 ANIMSLGGIAIAIGAMVDAAVVMIENAHKHIEEWKHRHPGQPLQGGNHWRVIGEAAAEVG 447 N+M+LGG+A+++G +VD A+V IEN +H+ H G+PL H +I A E+G Sbjct: 384 LNLMTLGGLALSVGILVDQAIVTIENIERHL------HLGKPL----HDAII-VGAGEIG 432 Query: 448 PTLFFCLLIITLSFIPVFTLEAQEGRLFGPLAFTKTYAMAGAAGLSVTLIPILMGYMI-- 505 F L I + F+P+F L LF PLA +AMA + LS TL+P L+ ++ Sbjct: 433 VPAFVATLCICIVFVPMFFLSGVARFLFVPLAEAVVFAMAASYLLSRTLVPTLVMLLMGS 492 Query: 506 RGKIPSENSNPLNRWLIRCYRPLLEAV------------LRRPRTTLAIAALALLTT-LW 552 G++ S + L R L R + E V R R LA LL+ L+ Sbjct: 493 HGQVDSAKQSLLQR-LYRAFDNRFERVRHGYTLVLSSLLAHRRRFALAFLGFCLLSCLLY 551 Query: 553 PISRLGGEFLPQMDEEDL---LYMPSALPGLSASKAAELLQQTDRLIKTVPEVASVFGKA 609 P LG +F P +D + + MP+ ++AA+ +++ R + E+ ++ Sbjct: 552 PF--LGRDFFPNVDGGQIRLHMRMPTGTRIEETARAADEVERVIRTLIPASELETILDNL 609 Query: 610 GRAESA-----TDPAPLEMFETTIQFKPRDQWRPGMTPTKLVN-ELDRVVKVPGLSNIWV 663 G S ++ + + I ++ RP ++ EL R + PG+ + Sbjct: 610 GVPNSGINLSYSNAGTIGTLDGEILMALKEGHRPTEALVSMLRAELPR--RFPGI-EFFF 666 Query: 664 PPIRNRLDMLATGIKSPIGVKVSGTSLADIDKTAQQIEQVAKEVPGVSSALAERLSGGRY 723 P +L G+ + I V+ +G ++ + A ++ + +++PG A + G Sbjct: 667 QPADIVTQILNFGLPAAIDVQFTGPNMEANARLAAELTKQIRQIPGAVDAHVHQRLDGPS 726 Query: 724 VDVDIDRLTAANYGLNIADVQSIVSSAIGGENVGETIEGL-----ARYPISVRYPRETRD 778 +D+ +DR +GL+ ++V + A+ G + + L Y I+V+ P+ D Sbjct: 727 LDLRMDRTRLQQFGLSASNVGQNLLIALAGSSQTQPAFWLNPNNGVVYNIAVQSPQYQVD 786 Query: 779 SLERLRDLPILTVSGQQIT----LGTVAKLKISDGPPMLKSENARPSGWIYVDVRGRDLS 834 SL+ L ++P+ + T LG + + + N P+ +YV V+G DL+ Sbjct: 787 SLDALLNIPVSPGANASTTPPQLLGNLVDVHAGRQMAVASRYNIAPAIDVYVSVQGTDLA 846 Query: 835 SVVADLRAAVSS-KVKLTPGLSIAYSGQFEYLERANARLKLVVPATLMIIFVLLYLTFRR 893 SV + A V K KL G + GQ E ++ + L + + ++++++L+ + F+ Sbjct: 847 SVAGKVSALVEQIKPKLARGSQVVIRGQVETMQSSFTGLGVGLAMAIVLVYLLVVVNFQS 906 Query: 894 FDEAALIMASLPFALVGGIWFLYAQGYNLSVATGVGFIALAGVSAEFGVVMLLYLKQAVS 953 + +AA+I+A+LP AL G W L+ G LSV G I GV+ ++++ + +Q Sbjct: 907 WIDAAIIIAALPAALAGIAWMLFITGTTLSVPALTGAIMTMGVATANSILIVAFARQ--- 963 Query: 954 AREQQGGTISVPVLEDAIREGAVLRVRPKAMTVAVILAGLIPILWGSGAGSEVMSRIAAP 1013 R ++G T L A+ G+ R+RP MT ++ G+IP+ G G G+E + + Sbjct: 964 -RREEGAT----PLAAALEAGST-RIRPVLMTALAMIIGMIPMALGLGEGAEQNAPLGRA 1017 Query: 1014 MVGGMITAPLLSMFVIPAAY 1033 ++GG++ A + ++F +P + Sbjct: 1018 VIGGLMLATVSTLFFVPVVF 1037 >gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 225 bits (574), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 257/1052 (24%), Positives = 477/1052 (45%), Gaps = 90/1052 (8%) Query: 21 LFLGGWGLWALKTTPVDALPDLSDVQVIIRTTYAGQAPQIVENQVTYPLATTMLSVPGAK 80 + L G +W L PV LP + + + G +P+ + + V PL + G Sbjct: 21 ILLVGVAVWPL--LPVAPLPQVDFPTIQVTAQLPGGSPETMASNVAQPLERQFSLIAGLS 78 Query: 81 TVRGYSFFGDSFVYVLFEDGTDMYWARSRVLEYLNQVQGRLP-----PSAKASLGP-DAT 134 + S G + + + F+ ++ A V +N G+LP P + P D+ Sbjct: 79 QMTSQSTLGSTQITLQFDLNRNIDAAALDVQAAINASTGQLPANLPNPPTFRKINPADSP 138 Query: 135 GVGWIYEYALVDRTGQHDLAQLRSLQDWFLKYELKSLPNVAEVATVGGMVKQYQVVLDPV 194 + + + T +D A D L ++ + V V G ++ +DP Sbjct: 139 IMIMSVQSEALPLTVVNDYA------DNILAQQISQISGVGLVNVNGQQKPAVRIQVDPA 192 Query: 195 KLAAYNIAQDKV--VDAIKKANQETGGSVLEMAETEYMVRASGYLKTLADFRNIPLATGR 252 KLA ++ + + V A NQ G ++ A + V + L + ++ LA R Sbjct: 193 KLATLGLSLEDIRGVIATTTVNQPKG--TVDGARQAFTVYTNDQLLKAEQWNDMVLA-WR 249 Query: 253 NGVPVKLGDVAIIQFGPEMRR----------GIAELN-GEGESVGGVIILRSGKNARETI 301 NG P+++ D+ I GPE + A+ + G S+ I + G N ET+ Sbjct: 250 NGAPIRVKDIGIAVDGPENNKIAAWAFAGAANTADHSITNGRSIVLAITKQPGANVIETV 309 Query: 302 VAVKSRLEELKKSLPAGVEVVPVYDRSQLIDRAIDNLSHKLLEEFIVVAVVCGLFLWHLR 361 +K+ + L+ ++PA + V + DR+Q I +++++ L+ +V +V +FL ++ Sbjct: 310 GRIKAAMPRLRAAIPAAIHVNTLIDRTQTIRASVEDVEFTLVLTIALVVMVIFVFLRNVA 369 Query: 362 SALVAIVSLPLGIMIAFIVMRYQGVNANIMSLGGIAIAIGAMVDAAVVMIENAHKHIEEW 421 + L+ V++PL IM VM G + + +SL + IA+G +VD A+VM+EN ++++EE Sbjct: 370 ATLIPSVTVPLAIMGTAGVMYVVGYSLDNLSLMALTIAVGFVVDDAIVMLENIYRYVEEG 429 Query: 422 KHRHPGQPLQGGNHWRVIGEAAAEVGPTLFFCLLIITLSFIPVFTLEAQEGRLFGPLAFT 481 PL+ + A E+G T+ + + FIP+ + GRLF A T Sbjct: 430 M-----TPLEAAL------KGAGEIGFTIISISVSLVAVFIPLLLMGGIVGRLFREFAVT 478 Query: 482 KTYAMAGAAGLSVTLIPILMGYMIRGKIPSENSNPLNRWLIRCYRPL-------LEAVLR 534 T +A + +S+TL P+L ++ + + L +W RC+ + L+ V++ Sbjct: 479 VTLTIAVSVIISLTLTPMLCSRFLKNE-HGQRHGKLYQWFERCFDAMLAGYKRGLDVVMK 537 Query: 535 RPRTTLA--IAALALLTTLWPISRLGGEFLPQMDEEDLLYMPSALPGLSASKAAELLQQT 592 TL IA LAL L+ G F PQ D ++ + S++ E L + Sbjct: 538 HQFITLMSFIATLALTAVLFIFIPKG--FFPQQDNGVIVGFAESAQDTSSAAMHERLLKV 595 Query: 593 DRLIKTVPEVASVFGKAGRAESATDPAPLEMFETTIQFKPRDQWRPGMTPTKLVNELDRV 652 +++ P+V AG T + F I KP+DQ R + +V Sbjct: 596 AEVVRQDPDVIGFAMSAGSTTFNTG----QFF---IALKPKDQGRTTNADEIIARLRPKV 648 Query: 653 VKVPGLSNIWVPPIRNRLDMLATGIKSPIGVKVSGTSLADIDKTAQQIEQVAKEVPGVSS 712 ++ G+ N+++ ++ +++ ++ ++ T L +++ A ++ + +++P ++ Sbjct: 649 ARIEGV-NLFMQASQD-INVGGRLSRTQYQYTITDTDLDELNVWAPRMLERFRQLPQLTD 706 Query: 713 ALAERLSGGRYVDVDIDRLTAANYGLNIADVQSIVSSAIGGENVGETIEGLARYPISVRY 772 +++ + + IDR A+++G++ + + + + AIG V + + Y R Sbjct: 707 VASDQQNQAAAAVITIDRARASSFGISPSLIDATIYDAIGQRQVAQYFTQINSY----RV 762 Query: 773 PRETRDSLER---LRDLPILT--VSGQQITLGTVAKLKISDGPPMLKSENAR-PSGWIYV 826 E +L++ L D LT ++GQQ+ L T K+ S + S ++ P+ I Sbjct: 763 ILEVTPALQKDPALFDKLYLTSPLTGQQVPLSTFVKIDTSKTSYLSISHQSQFPAVTISF 822 Query: 827 DV-RGRDLSSVVADLRAAVSSKVKLTPGLSIAYSGQFEYLERAN----ARLKLVVPATLM 881 ++ +G L V V K K G+ G F+ +A + ++ A L+ Sbjct: 823 NLAKGVSLGEAV-----DVIQKTKDQMGVPQTLVGNFQGTAKAFGDSLSSQPYLIAAALI 877 Query: 882 IIFVLLYLTFRRFDEAALIMASLPFALVGGIWFLYAQGYNLSVATGVGFIALAGVSAEFG 941 ++++L L + + I+++LP A VG + L A GY+LSV +G I L G+ + G Sbjct: 878 AVYIVLGLLYESYIHPLTILSTLPSAGVGALLILMAGGYDLSVIALIGIILLIGIVKKNG 937 Query: 942 VVMLLYLKQAVSAREQQGGTISVPVLEDAIREGAVLRVRPKAMTVAVILAGLIPILWGSG 1001 ++M+ + A++A QG P E AI + +LR RP MT L +P++ G G Sbjct: 938 IMMIDF---ALTAERSQGMK---P--EQAIYQACLLRFRPIMMTTMCALLSGLPLMLGHG 989 Query: 1002 AGSEVMSRIAAPMVGGMITAPLLSMFVIPAAY 1033 AGSE+ + MVGG+I + L++F P Y Sbjct: 990 AGSELRRPLGYAMVGGLILSQALTLFTTPVVY 1021 >gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 217 bits (553), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 244/1043 (23%), Positives = 470/1043 (45%), Gaps = 71/1043 (6%) Query: 15 LVLFGVLFLGGWGLWALKTTPVDALPDLSDVQVIIRTTYAGQAPQIVENQVTYPLATTML 74 L++ +L +G + A + P+ ALP++ + + T Y G +P ++ + VT PL Sbjct: 16 LLMLAILLVG---IVAYRLLPLSALPEVDYPTIQVVTLYPGASPDVMTSSVTAPLERQFG 72 Query: 75 SVPGAKTVRGYSFFGDSFVYVLFEDGTDMYWARSRVLEYLNQVQGRLP-----PSAKASL 129 +PG K + S G S + + F + A V +N LP P + + Sbjct: 73 QMPGLKQMSSTSSGGASVITLQFNLDLSLDIAEQEVQAAINAGSNLLPSDLPMPPVYSKV 132 Query: 130 GPDATGVGWIYEYALVDRTGQHDLAQLRSLQDWFLKYELKSLPNVAEVATVGGMVKQYQV 189 P T + L + L +++ L D + ++ L V V+ GG ++ Sbjct: 133 NPADTPI-----ITLAVTSNTMPLPKVQDLIDTRIAQKISQLSGVGLVSLSGGQRPAVRM 187 Query: 190 VLDPVKLAAYNIAQDKVVDAIKKANQETGGSVLEMAETEYMVRASGYLKTLADFRNIPLA 249 L+P +AA + D V AI AN + + A+ L++ ++RN+ +A Sbjct: 188 QLNPRAVAALGLNLDDVRTAIGNANVNQAKGSFDGPTRASTIDANDQLRSADEYRNLIIA 247 Query: 250 TGRNGVPVKLGDVAIIQFGPEMRRGIAELNGEGESVGGVIILRSGKNARETIVAVKSRLE 309 +NG P+++ DVA + G E R A N + + I + G N + ++K L Sbjct: 248 Y-KNGAPIRVSDVADVVDGAENVRLGAWANTQPAVIVN-IQRQPGANVIAVVDSIKKILP 305 Query: 310 ELKKSLPAGVEVVPVYDRSQLIDRAIDNLSHKLLEEFIVVAVVCGLFLWHLRSALVAIVS 369 +L+ SLP ++V + DR+ I ++D++ +LL +V +V +FL ++ + ++ V+ Sbjct: 306 QLQASLPGAIDVHILTDRTTTIRASVDDVKFELLLSVALVVMVIFVFLRNVPATIIPSVA 365 Query: 370 LPLGIMIAFIVMRYQGVNANIMSLGGIAIAIGAMVDAAVVMIENAHKHIEEWKHRHPGQP 429 +PL ++ F +M G + N ++L + IA G +VD A+VMIEN ++IEE +P Sbjct: 366 VPLSLVGTFAIMYLAGFSINNLTLMALTIATGFVVDDAIVMIENISRYIEEGM-----KP 420 Query: 430 LQGGNHWRVIGEAAAEVGPTLFFCLLIITLSFIPVFTLEAQEGRLFGPLAFTKTYAMAGA 489 +Q + A ++G T+ + IP+ + GRLF A T ++ + Sbjct: 421 MQAAL------KGAEQIGFTIISLTFSLIAVLIPLLFMGDVVGRLFHEFAVTLAVSILIS 474 Query: 490 AGLSVTLIPILMGYMIRGKIPSEN-------SNPLNRWLIRCYRPLLEAVLRRPRTTLAI 542 A +S+TL P++ ++R + P E S +I Y +L+ VL R + TL + Sbjct: 475 AVVSLTLTPMMCARLLRHQ-PEEQQGWFYHKSQQFFDRIIARYGVMLQWVLARQKLTLLV 533 Query: 543 AALALLTTLWPISRLGGEFLPQMDEEDLLYMPSALPGLSASKAAELLQQTDRLIKTVPEV 602 AA L T+ + F P D + + A +S S E QQ +++ P V Sbjct: 534 AAATLGLTVLLYVFVPKGFFPLQDTGVIQGISEATQSISFSAMGEKQQQLAKVVLQDPAV 593 Query: 603 ASVFGKAGRAESATDPAPLEMFETTIQFKPRDQWRPGMTPTKLVNELD--RVVKVPGLSN 660 S+ G + A L I KP D ++ T ++ L +VPG++ Sbjct: 594 ESLTSFIGVDGTN---ATLNSGRMLINLKPHDVRE--VSATDVIRRLQGKLAAEVPGIT- 647 Query: 661 IWVPPIRNRLDMLATGIKSPIGVKVSGTSLADID---------KTAQQIEQVAKEVPGVS 711 +++ P+++ I+ + +L D D K +++Q E+ V+ Sbjct: 648 LYMQPVQD------LTIEDSVSRTQYQFTLEDADPAVLSTWVPKLIARLQQ-QSELADVA 700 Query: 712 SALAERLSGGRYVDVDIDRLTAANYGLNIADVQSIVSSAIGGENVGETIEGLARYPISVR 771 + L ++ G ++IDR A+ G+ A + + + +A G + +Y + + Sbjct: 701 TNLQDQ---GLQAYIEIDRNAASRLGITTAAIDNALYNAFGQRLISTIYTQSNQYRVVME 757 Query: 772 YPRETRDSLERLRDLPILTVSGQQITLGTVAKLKISDGPPMLKSENARPSGWIYVDV-RG 830 E + L + ++T G ++ L ++A + P ++ P+ + ++ +G Sbjct: 758 VKPEFQHGPAALDSIYLVTSGGGKVPLSSIATVTERTAPLVVNHIGQFPAATVSFNLAKG 817 Query: 831 RDLSSVVADLRAAVSSKVKLTPGLSIAYSGQFEYLERANARLKLVVPATLMIIFVLLYLT 890 L V ++AA ++ + + G + + + ++ A ++ ++++L + Sbjct: 818 ASLGEAVKAIKAA-EEEIGMPASTVTNFQGAALAFQASLSNTLWLILAAIVTMYIVLGVL 876 Query: 891 FRRFDEAALIMASLPFALVGGIWFLYAQGYNLSVATGVGFIALAGVSAEFGVVMLLYLKQ 950 + + I+++LP A VG + L G +L + +G I L G+ + ++M+ + Sbjct: 877 YESYIHPITILSTLPSAGVGALLSLMISGTDLGIIGIIGIILLIGIVKKNAIMMIDF--- 933 Query: 951 AVSAREQQGGTISVPVLEDAIREGAVLRVRPKAMTVAVILAGLIPILWGSGAGSEVMSRI 1010 A+ A +G + P +AI + +LR RP MT L G +P++ G+G GSE+ + Sbjct: 934 ALEAERHEGKS---P--REAIYQACLLRFRPILMTTMAALLGALPLMLGTGVGSELRQPL 988 Query: 1011 AAPMVGGMITAPLLSMFVIPAAY 1033 MVGG++ + +L++F P Y Sbjct: 989 GITMVGGLLVSQVLTLFTTPVIY 1011 >gi|300313244|ref|YP_003777336.1| multidrug efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1033 Score = 213 bits (541), Expect = 9e-56, Method: Compositional matrix adjust. Identities = 244/1065 (22%), Positives = 459/1065 (43%), Gaps = 99/1065 (9%) Query: 14 FLVLFGVLFLGGWGLWALKTTPVDALPDLSDVQVIIRTTYAGQAPQIVENQVTYPLATTM 73 F+VLFGV+ A+ P+ P ++ V I TY G PQ + + V ++ Sbjct: 18 FIVLFGVI--------AMSQLPIARYPSVAPPTVSINATYPGATPQTMNDGV---ISLIE 66 Query: 74 LSVPGAKTVRGYSFFGDSF----VYVLFEDGTDMYWARSRVLEYLNQVQGRLPPSAKAS- 128 + G K + + D+ + V F+ GTD A+ V L ++ RLP + + + Sbjct: 67 RELSGVKNLLYFESSADTSGTAQISVTFKPGTDPELAQVDVQNKLKAIEARLPQTVRQNG 126 Query: 129 LGPDATGVGWIYEYALVDRTGQHDLAQLRSLQDWFLKYELKSLPNVAEVATVGGMVKQYQ 188 + ++ ++ +L G++D +L + EL+ + V V G + + Sbjct: 127 VTVESAASSFLLMVSLTSVDGRYDEVRLNDYLARNIVEELRRMEGVGRVQLFGAE-QAMR 185 Query: 189 VVLDPVKLAAYNIAQDKVVDAIKKANQETGGSVLEMA------ETEYMVRASGYLKTLAD 242 + LDP KL AY + + AI + N + L A + + G L++ A Sbjct: 186 IWLDPFKLNAYGLTVTDISTAISQQNAQIAPGRLGDAPAVRGQQIAMPLTVQGQLQSPAQ 245 Query: 243 FRNIPLATGRNGVPVKLGDVAIIQFGPEMRRGIAELNGEGESVGGVIILRSGKNARETIV 302 F I L +G V LGDVA ++ G + NG+ V V L G NA T Sbjct: 246 FAAIVLKAKADGSRVLLGDVARVELGAQSYAFANRENGKPAGVAAVQ-LSPGANAVRTAA 304 Query: 303 AVKSRLEELKKSLPAGVEVVPVYDRSQLIDRAIDNLSHKLLEEFIVVAVVCGLFLWHLRS 362 A+++R+EEL+ SLP G+ Y+ + + +I + L+E ++V +V LFL ++R Sbjct: 305 AIQARMEELRPSLPQGMAFSLPYNTAPFVKISIQKVVQTLIEAMVLVFLVMYLFLQNVRY 364 Query: 363 ALVAIVSLPLGIMIAFIVMRYQGVNANIMSLGGIAIAIGAMVDAAVVMIENAHKHIEEWK 422 ++ + P+ ++ VM+ G + N++++ G+ +AIG +VD A+V++EN + + Sbjct: 365 TVIPAIVAPIALLGTLAVMQLAGYSVNVLTMFGMVLAIGIIVDDAIVVVENVERIMAH-- 422 Query: 423 HRHPGQPLQGGNHWRVIGEAAAEVGPTLFFCLLIITLSFIPVFTLEAQEGRLFGPLAFTK 482 +G +A E+ + L++T FIP+ G ++ + Sbjct: 423 --------EGLGPREATIKAMGEITGAIIGITLVLTAVFIPMALAGGSVGVIYRQFSVAM 474 Query: 483 TYAMAGAAGLSVTLIPILMGYMIRGKIPSENSNP----------LNRWLIRCYRPLLEAV 532 ++ +A L+++L P L ++R +P+ + + NR R L + Sbjct: 475 AVSILFSAFLALSLTPALCATLLR-PVPAGHHDKRGFFGAFNRAFNRLTARYESALGRLI 533 Query: 533 LRRPRTTLAIAALALLTTLWPISRLGGEFLPQMDEEDLLYMPSALPGLSASKAAELLQQT 592 R R L AAL+ L + + L FLP+ D+ + + + A + Q Sbjct: 534 ARGLRMMLVYAALSGLLVVG-MQALPSAFLPEEDQGYFMTSFQLPADATTERTARAVDQF 592 Query: 593 DRLIKTVPEVASVFGKAGRAESATDPAPLEMFETTIQFKPRDQW--RPGMTPTKLVNELD 650 +R +K ++ + G S + P + F W R G T Sbjct: 593 ERYLKGREDIGANLSVLGFGFSGSGP------NAAMAFTELKDWKERKGSTSAAEAMAAQ 646 Query: 651 RVVK--VPGLSNIWVPPIRNRLDMLATGIKSPIGVKVSGTSLADIDKTAQQIEQV----- 703 ++ V G+ +PP L SG ++ D+ Q + Sbjct: 647 AAMESGVDGMVMSLLPPAIEELG------------NSSGFAMRLQDRANQGYAALKAAEA 694 Query: 704 --------AKEVPGVSSALAERLSGGRYVDVDIDRLTAANYGLNIADVQSIVSSAIGGEN 755 ++++ GV S + L G V ++IDR AA G++ + + +S+A+G Sbjct: 695 QLLALAAKSRKLSGVYS---DGLPAGASVQLEIDRHKAAALGVSFSSISDTLSAAMGSFY 751 Query: 756 VGETIEGLARYPISVRYPRETRDSLERLRDLPILTVSGQQITLGTVAKLKISDGPPMLKS 815 V + + ++ R LE + L + SG + L + K + P L+ Sbjct: 752 VNDFPNAGRMQQVILQAQASARMQLEDVMKLYVRNQSGGMVPLAELVKASWREAPTQLQ- 810 Query: 816 ENARPSGWIYVDVRGRDLSSVVADLRAAVSSKV--KLTPGLSIAYSGQFEYLERANARLK 873 R G+ V + G V + A ++ +L PG ++A++GQ + A+ Sbjct: 811 ---RYQGFPAVRISGSAAPGVASGEAMAEMERLAQQLPPGFAVAWTGQSLQERLSAAQAP 867 Query: 874 LVVPATLMIIFVLLYLTFRRFDEAALIMASLPFALVGGIWFLYAQGYNLSVATGVGFIAL 933 +++ +++++F++L + + +M +P LVG + + + V VG I + Sbjct: 868 MLMALSMLVVFLVLAALYESWSIPLSVMLVVPLGLVGAVAAVILRDMPNDVFFKVGMITV 927 Query: 934 AGVSAEFGVVMLLYLKQAVSAREQQGGTISVPVLEDAIREGAVLRVRPKAMTVAVILAGL 993 G+SA+ ++++ + +Q S E G ++E AI+ +VLR+RP MT G+ Sbjct: 928 IGLSAKNAILIVEFARQLRS--EGMG------LVEAAIK-ASVLRLRPILMTSLAFALGV 978 Query: 994 IPILWGSGAGSEVMSRIAAPMVGGMITAPLLSMFVIPAAYRLMRG 1038 +P++ SGA +E I + GGM++A +L++ +P + L+ G Sbjct: 979 VPLMLASGASAETQRAIGTGVFGGMVSATVLAVLFVPVFFVLVLG 1023 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 3,200,062 Number of extensions: 137310 Number of successful extensions: 572 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 498 Number of HSP's successfully gapped: 14 Length of query: 1046 Length of database: 1,619,870 Length adjustment: 95 Effective length of query: 951 Effective length of database: 1,170,045 Effective search space: 1112712795 Effective search space used: 1112712795 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 136 (57.0 bits)