BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0006.p (494 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [... 66 6e-12 gi|300309609|ref|YP_003773701.1| transport/efflux transmembrane ... 60 3e-10 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 60 3e-10 gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbas... 59 8e-10 >gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [Herbaspirillum seropedicae SmR1] Length = 399 Score = 65.9 bits (159), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 53/185 (28%), Positives = 90/185 (48%), Gaps = 14/185 (7%) Query: 218 IAAPISGLIQTLDIRSGMTVSAGA---PLARINGLDTVWLEAAIPE---------AQAAL 265 I AP+SG I ++ G V+ GA PL + + V+ + E A+AA Sbjct: 187 ITAPVSGRISRAEVTVGNVVNPGAANAPLTTLVSVAKVYASFDVDEQSFLKYVNPARAAG 246 Query: 266 VNVGGKLEADFAAYSGEKFTGKILAVLPESNADSRTLRVRAELPNRDGKLKPGMYAQIRL 325 V +E A G G++ +V + S T+RVRA N + +L PG+YA++RL Sbjct: 247 ATV--PVELGLANEDGYSRKGRVGSVDNRLDTASGTIRVRAIFDNANSQLVPGLYARVRL 304 Query: 326 GGGKAAPSLLVPSEAVIRTGARNILLVTTGDGRYQPVEVKLGQESDGKSIILDGVTEGQQ 385 GGG+ ++L+ +A+ + ++V D +V +G DG +I G+ G++ Sbjct: 305 GGGEPHQAVLIDEKAIGTDQDKRFVMVADKDNHANYRQVVVGAGHDGLVVIEKGLQPGER 364 Query: 386 VVTSG 390 +V +G Sbjct: 365 IVVNG 369 >gi|300309609|ref|YP_003773701.1| transport/efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 376 Score = 60.5 bits (145), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 52/189 (27%), Positives = 85/189 (44%), Gaps = 2/189 (1%) Query: 217 TIAAPISGLIQTLDIRSGMTVSAGAPLARINGLDTVWLEAAIPEAQAALVNVGGKLEADF 276 T+ A G+I +G ++AG P+ + V + + + Q A + G Sbjct: 178 TLVAQHDGVISAEQANTGDVLAAGQPVYSLAWSGQVDVVTEVADRQVATLQPGAAAVVTL 237 Query: 277 AAYSGEKFTGKILAVLPESNADSRTLRVRAELPNRDGKLKPGMYAQIRL--GGGKAAPSL 334 AA G+ FTGK+ V P ++A SRT RV+ L D ++ GM ++ + AAP L Sbjct: 238 AALPGKTFTGKVREVSPAADAQSRTYRVKVTLEQPDPAVRLGMTGEVAITPAASSAAPVL 297 Query: 335 LVPSEAVIRTGARNILLVTTGDGRYQPVEVKLGQESDGKSIILDGVTEGQQVVTSGQFLI 394 L+P+ A+ G R L V G+ + V + + + GVT +VV G + Sbjct: 298 LLPATALFHQGDRPALWVVGEQGQLELRPVTVAAYGERSISVSQGVTASDKVVVQGVHTL 357 Query: 395 DSEASLKGV 403 + +K V Sbjct: 358 TAGEKVKPV 366 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 60.5 bits (145), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 62/223 (27%), Positives = 105/223 (47%), Gaps = 18/223 (8%) Query: 217 TIAAPISGLIQTLDIRSGMTVSAG--APLARINGLDTVWLEAA--------IPEAQAA-- 264 T+ +PISG I + G V G LA + LD V++ + +A AA Sbjct: 174 TVTSPISGRIGRALVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQ 233 Query: 265 LVNVG-GKLEADFAAYSGEKFT--GKILAVLPESNADSRTLRVRAELPNRDGKLKPGMYA 321 L +VG K +G+ + GK+L + ++ ++ +RAE PN L PG Y Sbjct: 234 LKSVGDNKASVTLELENGQAYPLPGKLLFSDISVDENTGSVSLRAEFPNPKRLLLPGTYV 293 Query: 322 QIRLGGGKAAPSLLVPSEAVIRTGARNILLVTTGDGRYQPVEVKLGQESDGKSIILDGVT 381 + ++ +LLVP +AV+R + ++V + + +P VK G +LDG+ Sbjct: 294 RAKIEQAVDEQALLVPQQAVVRDAGGSTVMVVNAENKVEPRPVKTGGARGNNWQVLDGLK 353 Query: 382 EGQQVVTSGQFLIDSEASLKGVLARLNTSAGADAKSAQMPLNE 424 EG +V+ G + A++K V + N AGA A+++ P + Sbjct: 354 EGDRVIVEGLQKVRPGATVKPV--QWN-PAGAPAQASGQPAKQ 393 >gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 58.9 bits (141), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 58/192 (30%), Positives = 89/192 (46%), Gaps = 15/192 (7%) Query: 214 TVVTIAAPISGLIQTLDIRSGMTVSA--GAPLARINGLDTVWLEAAIPEAQAALVNVGGK 271 + TI APISG + + G V A L IN +D + L +PE A +N + Sbjct: 183 SYTTINAPISGRVGARLVDPGNIVHATDANGLVVINQIDPITLVFTLPEEAVAGINRAQR 242 Query: 272 LEAD---FAAYSGEK----FTGKILAVLPESNADSRTLRVRAELPNRDGKLKPGMYAQIR 324 +AY+ + TGK++ + + N + T++++A N D KL PG Y R Sbjct: 243 AHQQPLQVSAYARDSQEVLATGKLVLLDNQINTTNGTVQLKARFDNPDHKLWPGQYVNAR 302 Query: 325 LGGGKAAPSLLVPSEAVIRT--GARNILLVTTGDGRYQPVEVKLGQESDGKSIILDG--V 380 L +L VP+ A+ R G + R QPV+V+ Q DG +++ G V Sbjct: 303 LQMRSHGNALTVPAAAIQRNQQGTYVYAIDQEDQARMQPVKVERIQ--DGIAVLAAGNAV 360 Query: 381 TEGQQVVTSGQF 392 GQ+VV GQ+ Sbjct: 361 QAGQRVVIDGQY 372 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,594,536 Number of extensions: 70945 Number of successful extensions: 291 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 287 Number of HSP's successfully gapped: 9 Length of query: 494 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 405 Effective length of database: 1,198,455 Effective search space: 485374275 Effective search space used: 485374275 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)