BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0241.p (104 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309956|ref|YP_003774048.1| anti-sigma-factor antagonist (S... 124 1e-30 >gi|300309956|ref|YP_003774048.1| anti-sigma-factor antagonist (STAS) domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 102 Score = 124 bits (312), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 78/104 (75%), Positives = 86/104 (82%), Gaps = 2/104 (1%) Query: 1 MPLTYSVQEDRLLLTVSGGLTIFQAAERKPELLHALTLPQTQVVLDLSGVDELDTAGIQL 60 MP+T +VQED+LLL VSGGLTIFQAAERKPELLH LTL Q QV LDL+GVDE+DTAGIQL Sbjct: 1 MPVTLTVQEDKLLLQVSGGLTIFQAAERKPELLHVLTLAQPQVQLDLAGVDEIDTAGIQL 60 Query: 61 LLLLRRELAAGGRQLEIGVYSPAVLATLDLLQLHERFSPAVEAA 104 LLLLRREL A GR L+I SPA+L DLLQLHERF +EAA Sbjct: 61 LLLLRRELRAQGRSLDIVGASPALLVAFDLLQLHERF--GMEAA 102 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 259,379 Number of extensions: 6926 Number of successful extensions: 30 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 30 Number of HSP's successfully gapped: 1 Length of query: 104 Length of database: 1,619,870 Length adjustment: 71 Effective length of query: 33 Effective length of database: 1,283,685 Effective search space: 42361605 Effective search space used: 42361605 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 123 (52.0 bits)