BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0215.p (151 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309530|ref|YP_003773622.1| general secretion pathway prote... 248 1e-67 >gi|300309530|ref|YP_003773622.1| general secretion pathway protein [Herbaspirillum seropedicae SmR1] Length = 183 Score = 248 bits (634), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 123/155 (79%), Positives = 133/155 (85%), Gaps = 4/155 (2%) Query: 1 MRRSL----RHPAFLFLQRGLARLRWILALTVLAVAAALLLPPLVTRFNHDHSPQALAAE 56 MRRSL RHPAFLFLQRGLARLRWIL LTVLAV AALL+PPLVTRFNHDHSPQA AAE Sbjct: 29 MRRSLQPSRRHPAFLFLQRGLARLRWILVLTVLAVGAALLVPPLVTRFNHDHSPQAQAAE 88 Query: 57 KDMQAIAAGLERYRQDNGRYPSAAQGLLALVIKPTRAPVPGNWSIGGYVDRLPRDPWGNS 116 KDM+AIAAGLERYRQDNGRYPS QGLLALVI+P R P+ W +GGYV+RLPRDPWGN Sbjct: 89 KDMKAIAAGLERYRQDNGRYPSTQQGLLALVIRPVREPLANGWQLGGYVERLPRDPWGNP 148 Query: 117 YQYGADDDGTSYELYSFGQAGPDGGADGEQVIRQH 151 YQY ADDDG SYEL+SFGQAGP+GG D ++VI H Sbjct: 149 YQYQADDDGASYELFSFGQAGPEGGDDDDRVISLH 183 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 521,081 Number of extensions: 22346 Number of successful extensions: 67 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 67 Number of HSP's successfully gapped: 1 Length of query: 151 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 74 Effective length of database: 1,255,275 Effective search space: 92890350 Effective search space used: 92890350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)