BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0201.p (428 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 779 0.0 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 385 e-108 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 384 e-108 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 282 4e-77 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 278 7e-76 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 779 bits (2012), Expect = 0.0, Method: Compositional matrix adjust. Identities = 407/433 (93%), Positives = 415/433 (95%), Gaps = 5/433 (1%) Query: 1 MQATTLPAGGIPVVTNHQRRRAIIATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTS 60 MQA+TLPA GI V+N QRRRAI+ATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTS Sbjct: 1 MQASTLPADGIVSVSNQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTS 60 Query: 61 LLLAVATFGVGFFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESVGL 120 LLLAVATFGVGFFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYES+GL Sbjct: 61 LLLAVATFGVGFFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGL 120 Query: 121 FAPLIIVVARLLQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGT 180 FAPLIIVVARLLQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGT Sbjct: 121 FAPLIIVVARLLQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGT 180 Query: 181 FVTTSLDPQALASWGWRLPFLLGIIIGPVGYYIRNHLEETPTFLEEKDKTDSPLKEVIRN 240 FVT SLD ALASWGWRLPFLLGIIIGPVGYYIR+HLEETPTFLEEKDKTDSPLKEVIRN Sbjct: 181 FVTASLDAAALASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTDSPLKEVIRN 240 Query: 241 FPRETSASFSMVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVG 300 FPRETSASFSMVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVG Sbjct: 241 FPRETSASFSMVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVG 300 Query: 301 RL-DMGLERP----AAALILVLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAA 355 RL D RP +AALIL+LAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAA Sbjct: 301 RLADRIGRRPLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAA 360 Query: 356 FSELFPAKVLSTGLSVAYNLAVTIFGGFASFFITWLIAATGNNMAPAFYVMIAAAISFVG 415 FSELFPAKVLSTGLSVAYNLAVTIFGGFASFFITWLIAATG+ MAPAFYVMIAAAISFVG Sbjct: 361 FSELFPAKVLSTGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVG 420 Query: 416 TRFVREPQYLQQH 428 TRFVREPQYLQ H Sbjct: 421 TRFVREPQYLQPH 433 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 385 bits (990), Expect = e-108, Method: Compositional matrix adjust. Identities = 199/412 (48%), Positives = 272/412 (66%), Gaps = 8/412 (1%) Query: 18 QRRRAIIATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFFMRPV 77 ++R +IA ++GN LEW+DF VY FF+ IIA+LFFP N+ TSLL+A+ATFG+GFFMRPV Sbjct: 6 NKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFFMRPV 65 Query: 78 GGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESVGLFAPLIIVVARLLQGFSA 137 GG+LLG+YADR GRKAA+ V I+LM + +I PTY ++G+ AP++IVVAR+LQGF+ Sbjct: 66 GGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFAT 125 Query: 138 GGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTTSLDPQALASWGWR 197 GGE S+TAFL E AP +R Y SW +AV GA +G VT +L P+AL SWGWR Sbjct: 126 GGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGWR 185 Query: 198 LPFLLGIIIGPVGYYIRNHLEETPTFLEEKDKTD-SPLK--EVIRNFPRETSASFSMVIL 254 LPF+LG++IGPVG +IR H+ ET FLE +K + +P+K +V++ R S VI Sbjct: 186 LPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSMGQVIN 245 Query: 255 WTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLDMGLERPAAAL- 313 TV YV+L MPT++ K L LP F+ M+ + + P G L L R L Sbjct: 246 GTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRRTVLLY 305 Query: 314 ----ILVLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLSTGL 369 L++ YP+F ++++APSL LL+ Q++ +I A GP+ A SE FP +V STGL Sbjct: 306 SSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVRSTGL 365 Query: 370 SVAYNLAVTIFGGFASFFITWLIAATGNNMAPAFYVMIAAAISFVGTRFVRE 421 +VAYNLA +FGGFA F +TWL +G+ +APA+YV+ AA I +R+ Sbjct: 366 AVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVSAAWAMRD 417 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 384 bits (987), Expect = e-108, Method: Compositional matrix adjust. Identities = 197/412 (47%), Positives = 273/412 (66%), Gaps = 7/412 (1%) Query: 14 VTNHQRRRAIIATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFF 73 +T+ Q RRAII++ IGN LEWFD +Y FAV+IAK FFPTG+D SLLL ATFGV FF Sbjct: 12 ITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFGVSFF 71 Query: 74 MRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESVGLFAPLIIVVARLLQ 133 MRP+G ++LG Y+DR GRKAAL ++I+LM VGT MI P+Y S+GL AP I + +++Q Sbjct: 72 MRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQ 131 Query: 134 GFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTTSLDPQALAS 193 GFSAGGE GS+TAFL E+AP R R ++SSW AS G+++LL A G + L P+ LAS Sbjct: 132 GFSAGGEFGSSTAFLVEHAPHR-RGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQLAS 190 Query: 194 WGWRLPFLLGIIIGPVGYYIRNHLEETPTFLEEKDKTDSPLKEVIRNFPRETSASFSMVI 253 WGWR+PF+ G++I P G YIR HL+E P F E +KT++PL++ + VI Sbjct: 191 WGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQKMRLLIGAGSVI 250 Query: 254 LWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLDMGLER----- 308 + TV Y+ L Y+PTY+VK L LP + F A V GL + + +P+VG L + R Sbjct: 251 MATVSVYLSL-YIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTPFMI 309 Query: 309 PAAALILVLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLSTG 368 ++AL +VL YPMF ++ ++P LL+ Q V G L+ Y + A +++FP TG Sbjct: 310 TSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRGTG 369 Query: 369 LSVAYNLAVTIFGGFASFFITWLIAATGNNMAPAFYVMIAAAISFVGTRFVR 420 +S+AYN+AVTIFGGFA ITWLI TG+ ++ +++V+ A +S + + R Sbjct: 370 MSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASLSAR 421 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 282 bits (721), Expect = 4e-77, Method: Compositional matrix adjust. Identities = 157/419 (37%), Positives = 238/419 (56%), Gaps = 8/419 (1%) Query: 16 NHQRRRAIIATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFFMR 75 + + ++ GN +E FDF ++ F+A I+K FFP+ N+ SL+L TFG GF MR Sbjct: 7 SQSKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFGAGFLMR 66 Query: 76 PVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESVGLFAPLIIVVARLLQGF 135 P+G I+LG Y DRVGR+ L VT+ LMA+GT +I P+Y ++G APL++++ RLLQGF Sbjct: 67 PLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIGRLLQGF 126 Query: 136 SAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTTSLDPQALASWG 195 SAG E+G + +L E A + +Y SW AS VA+++ AA+G ++TSL P ++ WG Sbjct: 127 SAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPAEVSGWG 186 Query: 196 WRLPFLLGIIIGPVGYYIRNHLEETPTFLEEKDK--TDSPLKEVIRNFPRETSASFSMVI 253 WR+PF +G +I PV + IR L+ET F+ K + T + +I N+ + A +V Sbjct: 187 WRIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENW-KLVIAGMMLVS 245 Query: 254 LWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRL-DMGLERPA-- 310 + TV Y++ Y PT+ VLKL I + GL + PI+G L D RP Sbjct: 246 MTTVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGRRPLLV 305 Query: 311 --AALILVLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLSTG 368 L ++ AYP ++ S + +LI +L L A+Y G ++ A +E+ PA V + G Sbjct: 306 TFTVLTILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPADVRTAG 365 Query: 369 LSVAYNLAVTIFGGFASFFITWLIAATGNNMAPAFYVMIAAAISFVGTRFVREPQYLQQ 427 S+AY+LA +FGGF T LI T + AP ++ AA + T + + ++Q Sbjct: 366 FSLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIATLILYRNKAVRQ 424 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 278 bits (710), Expect = 7e-76, Method: Compositional matrix adjust. Identities = 159/408 (38%), Positives = 229/408 (56%), Gaps = 8/408 (1%) Query: 16 NHQRRRAIIATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFFMR 75 H + ++ GN LE FDF +Y F+A I+K FFP+ N+ SL+L TFG GF MR Sbjct: 9 KHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTFGAGFLMR 68 Query: 76 PVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESVGLFAPLIIVVARLLQGF 135 P+G I+LG Y DRVGR+ L VT+ +MA+GT +I P Y ++G+ AP++++ RLLQGF Sbjct: 69 PLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLTGRLLQGF 128 Query: 136 SAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTTSLDPQALASWG 195 SAG E+G + +L E A + +Y SW AS A++ A +G V+ SL P ++ WG Sbjct: 129 SAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPVQISDWG 188 Query: 196 WRLPFLLGIIIGPVGYYIRNHLEETPTFLEEKDKTD--SPLKEVIRNFPRETSASFSMVI 253 WR+PF +G +I P + IR L+ET FL K + + +N+ + A MV+ Sbjct: 189 WRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNW-KIVVAGMMMVV 247 Query: 254 LWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRL-DMGLERPA-- 310 + TV Y++ Y PT+ VLKL + I GL PI+G L D RP Sbjct: 248 MTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRVGRRPVLV 307 Query: 311 --AALILVLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLSTG 368 L L+ AYP+ +++ S S LLI +L L A+Y G + A +E+ PA V + G Sbjct: 308 AFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMPAHVRTVG 367 Query: 369 LSVAYNLAVTIFGGFASFFITWLIAATGNNMAPAFYVMIAAAISFVGT 416 S+AY+LA IFGGF TWLI +G+ AP +++ AA + T Sbjct: 368 FSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLAT 415 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,385,834 Number of extensions: 61463 Number of successful extensions: 393 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 359 Number of HSP's successfully gapped: 20 Length of query: 428 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 340 Effective length of database: 1,203,190 Effective search space: 409084600 Effective search space used: 409084600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)