BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0193.p (278 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309592|ref|YP_003773684.1| VGR-related protein [Herbaspiri... 434 e-123 gi|300309366|ref|YP_003773458.1| VGR-related protein [Herbaspiri... 120 2e-28 gi|300310274|ref|YP_003774366.1| VGR-related protein [Herbaspiri... 84 2e-17 gi|300313606|ref|YP_003777698.1| VGR-related type VI secretion s... 80 2e-16 gi|300309621|ref|YP_003773713.1| VGR-related protein [Herbaspiri... 60 2e-10 >gi|300309592|ref|YP_003773684.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 966 Score = 434 bits (1116), Expect = e-123, Method: Compositional matrix adjust. Identities = 220/277 (79%), Positives = 247/277 (89%) Query: 2 TDLAQLERIRKQLQAWDTSEAAGGTRHAAAPMVALDAPAGVSVTSQDTMVLGASRHIDLV 61 TD+AQLERIR QL+AWD + A G A PMVA+DAPAGVS+TSQDTMVLGA++HIDLV Sbjct: 673 TDVAQLERIRAQLRAWDAGDVANGAVRAETPMVAVDAPAGVSITSQDTMVLGAAQHIDLV 732 Query: 62 SRDNTQLNAGRKLLMRAGEMFAAFAGKHMKLISGKGSVKVQAHEEHVELQAARRILLEAS 121 SR NTQL+AGR+LLMRAGEM +AFA KHMKLISGKGS+K+QAHEEH++L AARRILLEAS Sbjct: 733 SRANTQLSAGRRLLMRAGEMLSAFAAKHMKLISGKGSIKIQAHEEHIDLSAARRILLEAS 792 Query: 122 EEIILQAPKISIRSRDDMQIHGGGSFSRWNASLIEHGTSGQWRQQAAEHNVLGPANCPPP 181 EEIILQAPKISIRSR+ ++ GGGSFSRWNAS IEHGTSG+WRQQAA H+VLGPANCPPP Sbjct: 793 EEIILQAPKISIRSRETTEVAGGGSFSRWNASAIEHGTSGEWRQQAAMHHVLGPANCPPP 852 Query: 182 EIAPPPTFEELQQSASLMVRLRSHVRDGRPLAHQPYTLFKQGTQIAVGVTDAAGRLRIEG 241 +I PPPT+EELQQS+SLMVRLRSH RDGR LAHQPYTLFKQG QIAVGVTDAAG+LRI+ Sbjct: 853 DIPPPPTYEELQQSSSLMVRLRSHARDGRLLAHQPYTLFKQGEQIAVGVTDAAGQLRIKD 912 Query: 242 HQPGDDSYSVKLPNGYCFDLPVHEQVSALDQQLAASG 278 HQPGDDSYSV+L NGY FDLPV E++ LD QLAASG Sbjct: 913 HQPGDDSYSVRLSNGYHFDLPVQEELGDLDHQLAASG 949 >gi|300309366|ref|YP_003773458.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 913 Score = 120 bits (300), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 80/237 (33%), Positives = 124/237 (52%), Gaps = 15/237 (6%) Query: 2 TDLAQLERIRKQLQAWDT-SEAAGGTRHAAAPMVALDAPAGVSVTSQDTMVLGASRHIDL 60 T L R+R QL AW+ S + PMVA+ APAGV++TS + + LGA + IDL Sbjct: 671 TQLQTFSRLRDQLDAWEAGSNTQAKSDGGGKPMVAVTAPAGVAITSDEGVALGAQKEIDL 730 Query: 61 VSRDNTQLNAGRKLLMRAGEMFAAFAGK-HMKLISGKGSVKVQAHEEHVELQAARRILLE 119 +S NTQ +AG+KLL R E FA + ++L++ G +++Q H VE+ +ARRI+L Sbjct: 731 ISVGNTQFSAGKKLLARVAESICLFAQRLGIQLVAANGKLQLQTHGGDVEVTSARRIVLT 790 Query: 120 ASEEIILQAPKISIRSRDDMQIHGGGSFSRWNASLIEHGTSGQWRQQAAEHNVLGPANCP 179 A++EI+LQAP++ ++++ GGG +I +SG ++ + + A+ Sbjct: 791 AADEIVLQAPRLRLQAQGAQIDLGGG--------VITQQSSGDHVIRSGNFHHVSGADSQ 842 Query: 180 PPEIAPPPTFEELQQSASLMVRLRSHVRDGRPLAHQPYTLFKQGTQIAVGVTDAAGR 236 PP + P + + L R GRP Q Y + + GVTD GR Sbjct: 843 PPGVELPASTMRTDERFILARR-----GSGRPHVRQRYRIELDDGSVIEGVTDEQGR 894 >gi|300310274|ref|YP_003774366.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 928 Score = 83.6 bits (205), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 61/224 (27%), Positives = 106/224 (47%), Gaps = 12/224 (5%) Query: 19 TSEAAGGTRHAAAPMVALDAPAGVSVTSQDTMVLGASRHIDLVSRDNTQLNAGRKLLMRA 78 + + G P++ + APAG+ + + + +++ L S +T + +G+ LL Sbjct: 666 SGQGQGSANRFKKPLLMMAAPAGIGLATDQGTQVHSAQETTLTSGKDTSIASGKSLLATV 725 Query: 79 GEMFAAFA-GKHMKLISGKGSVKVQAHEEHVELQAARRILLEASEEIILQAPKISIRSRD 137 E + FA MKL + KG V++QA + ++L A E ++ + ++ I ++D Sbjct: 726 KEKISLFAQNAGMKLFAAKGKVEIQAQSDDLDLIA------EKVARLLSTSGRVEIHAKD 779 Query: 138 DMQIHGGGSFSRWNASLIEHGTSGQWRQQAAEHNVLGPANCPPPEIAPPPTFEELQQSAS 197 ++ I GGSF + N++ I GT G+W +A+ H++ GPA+ + P ELQ S Sbjct: 780 EVMISAGGSFIQINSAGITQGTPGKWEAKASLHSMPGPAS--QNFVMPHVQKSELQPSD- 836 Query: 198 LMVRLRSHVRDGRPLAHQPYTLFKQGTQIAVGVTDAAGRLRIEG 241 + R G PLA Y + G DA G R+ G Sbjct: 837 --MEFRHLTDWGEPLAGAAYKAILSDGSVRKGTLDAQGIARLSG 878 >gi|300313606|ref|YP_003777698.1| VGR-related type VI secretion system protein [Herbaspirillum seropedicae SmR1] Length = 886 Score = 79.7 bits (195), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 64/204 (31%), Positives = 102/204 (50%), Gaps = 21/204 (10%) Query: 38 APAGVSVTSQDTMVLGASRHIDLVSRDNTQLNAGRKLLMRAGEMFAAFAGKH-MKLISGK 96 +P G+ +T+ + A I L S +T + G+ LL E + FA K +KL S K Sbjct: 675 SPDGIGLTTPKGVHTHAGGEITLSSGADTSIAVGKSLLASVAEKISLFAFKAGIKLFSAK 734 Query: 97 GSVKVQAHEEHVELQAARRILLEASEEIILQAPKISIRSRDDMQIHGGGSFSRWNASLIE 156 G V+VQA + +EL A E ++ + ++ IR+++++ I GGSF R NAS I Sbjct: 735 GKVEVQAQSDDLELIA------EKVARLLSASGRVEIRAKEEVLITAGGSFIRLNASGIT 788 Query: 157 HGTSGQWRQQAAEHNVLGPANCPPPEIAPPPTFEELQQSASLMVRLRSHVR---DGRPLA 213 GTSG W +A H + GP +E + SA+L +R D P+ Sbjct: 789 QGTSGAWEAKAGTHAMPGPTTL---------AYEMNKSSAALPFDEEFVLRWPYDQSPVK 839 Query: 214 HQPYTLFK-QGTQIAVGVTDAAGR 236 ++ + + + GT++ GVTDA G+ Sbjct: 840 NRRFEIVRGDGTKVR-GVTDAQGK 862 >gi|300309621|ref|YP_003773713.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 912 Score = 59.7 bits (143), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 59/198 (29%), Positives = 91/198 (45%), Gaps = 29/198 (14%) Query: 3 DLAQLERIRKQLQAWDT-SEAAGGTRHAAAPMVALDAPAGVSVTSQDTMVLGASRHIDLV 61 D A ER+ +QLQ W+ S AP+VA+ APAG+ ++ +T V A + D V Sbjct: 668 DHAPHERLHRQLQQWENGSNTKPDAGDGGAPVVAVTAPAGLHFSTPETAVTHAGENCDTV 727 Query: 62 SRDNTQLNAGRKLLMRAGEMFAAFAGK---------HMKLISGKGSVKVQAHEEHVELQA 112 + + QL +G E A AGK + LI+ +G +K+Q+ ++ A Sbjct: 728 ALRHIQLASG--------EHLTANAGKGISLFAQSEDLTLIAHQGELKLQSQHNDTKVAA 779 Query: 113 ARRILLEASEEIILQAPKISIRSRDDMQIHGG-GSFSRWNASLIEHGTSGQWRQQAAEHN 171 A+ + L AS K+ I + D++ + GS+ R I+ G SG AA H Sbjct: 780 AKNLSLTAS------GGKVQIIAADEILLAASDGSYLRLGGGKIDMGCSGAATIHAATHR 833 Query: 172 VLGPANCPPPEIAPPPTF 189 + A P E+ PTF Sbjct: 834 WI-DAQTTPGEL---PTF 847 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.131 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 775,395 Number of extensions: 28260 Number of successful extensions: 114 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 106 Number of HSP's successfully gapped: 6 Length of query: 278 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 194 Effective length of database: 1,222,130 Effective search space: 237093220 Effective search space used: 237093220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)