BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0192.p (375 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309592|ref|YP_003773684.1| VGR-related protein [Herbaspiri... 509 e-145 gi|300309366|ref|YP_003773458.1| VGR-related protein [Herbaspiri... 199 3e-52 gi|300309621|ref|YP_003773713.1| VGR-related protein [Herbaspiri... 121 8e-29 gi|300310083|ref|YP_003774175.1| VGR-related type VI secretion s... 100 2e-22 gi|300310071|ref|YP_003774163.1| VGR-related protein [Herbaspiri... 91 2e-19 >gi|300309592|ref|YP_003773684.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 966 Score = 509 bits (1310), Expect = e-145, Method: Compositional matrix adjust. Identities = 248/332 (74%), Positives = 271/332 (81%) Query: 12 MTDQFQTIAAAFAGAVLSQQHRPLRLRWRAQETHFAQWLLVQRVDIEEACLEGMQAKLTC 71 MTD F TI AAFA A+LSQ RP+RLRW QE F Q LL+QRVDI+E LEGMQ +LTC Sbjct: 1 MTDHFATITAAFASAILSQNLRPIRLRWGPQEQRFEQSLLLQRVDIQETLLEGMQVQLTC 60 Query: 72 VSSDPELPLQALLGQPLGIEMVTDRGGLHHTNGIITEARAGQADGALRVYQLTLKDALSI 131 VS+DP+LPL LLGQPLG+EMVTDRG LHHTNG+IT+A AG ADGALRVYQLT++DALSI Sbjct: 61 VSTDPDLPLHTLLGQPLGVEMVTDRGRLHHTNGLITDACAGHADGALRVYQLTMRDALSI 120 Query: 132 MEERINTRVFRQLSVPRILEIMLGEWRQRSPALATAFHFDLDGLEDRRYPVREISHQFNE 191 ME R+NTR+FR+LSVP ILEI+L EWRQRS LA AF FDLDGL+D RYP REISHQFNE Sbjct: 121 MEGRVNTRIFRRLSVPSILEILLSEWRQRSSTLAAAFKFDLDGLDDTRYPAREISHQFNE 180 Query: 192 SDAHFIRRLCRREGIAWYVASPQPTAETGRTEDADVPMHALVFCDQSALLPRALAGTVRY 251 SDAHFIRRLCRREGIAWYVAS Q ET AD+P+H LVFCDQ ALLPRA AGTVRY Sbjct: 181 SDAHFIRRLCRREGIAWYVASGQQADETAIATHADIPIHTLVFCDQPALLPRAAAGTVRY 240 Query: 252 HRDGATEERDTISAWSRQRSLVPGRVWRGSWDYKAVRVEEAASHSAIDQGDTGRALAAIL 311 HRDGATEERD ISAWSRQRSLVPG VWR SWDYKAVR EEA+SHS +DQGD G LA IL Sbjct: 241 HRDGATEERDAISAWSRQRSLVPGSVWRRSWDYKAVRAEEASSHSIVDQGDAGYKLANIL 300 Query: 312 NDGLIEAPHAADGQQDHLRLGMASCVGPKASS 343 DGLIEAPHAAD Q DHLRL + +A S Sbjct: 301 RDGLIEAPHAADSQNDHLRLADTRMLAHEARS 332 >gi|300309366|ref|YP_003773458.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 913 Score = 199 bits (507), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 119/306 (38%), Positives = 167/306 (54%), Gaps = 5/306 (1%) Query: 29 SQQHRPLRLRWRAQETHFAQWLLVQRVDIEEACLEGMQAKLTCVSSDPELPLQALLGQPL 88 +Q+ R +RLR LLV V E+ M+ +L C++S +L L+ + P+ Sbjct: 14 TQKARAIRLRLPPSHQALQDALLVMHVTGRESLCGAMEYELLCLTSREDLALEDFVALPV 73 Query: 89 GIEMVTDRGGLHHTNGIITEARAGQADGALRVYQLTLKDALSIMEERINTRVFRQLSVPR 148 I++VTD G L G I EA AGQ D L Y+L L+DALS+M RINTRVF + S Sbjct: 74 EIQLVTDLGLLRTVCGFIAEAHAGQLDDELVSYRLVLRDALSLMGHRINTRVFLRASEAD 133 Query: 149 ILEIMLGEWRQRSPALATAFHFDLDGLEDRRYPVREISHQFNESDAHFIRRLCRREGIAW 208 + + ++ EWRQRSP +A++F D L + R+P RE Q NESDA F+RRL +R GI+W Sbjct: 134 VTQALVSEWRQRSPLMASSFEVDWSLLAE-RFPAREFIMQHNESDADFLRRLWKRRGISW 192 Query: 209 YVASPQPTAETGRTEDADVPMHALVFCDQSALLPRALAGTVRYHRDGATEERDTISAWSR 268 ++ +P A P H LV D + LPR AG V Y +D A E D I W Sbjct: 193 FIRPGEPPL----PHQAGSPRHTLVLFDDAVQLPRNAAGAVSYRQDHAPAEEDGIVRWLG 248 Query: 269 QRSLVPGRVWRGSWDYKAVRVEEAASHSAIDQGDTGRALAAILNDGLIEAPHAADGQQDH 328 R L G V R +WD+ + ++ +QG+ G A +L+D IEAPH AD ++D Sbjct: 249 TRRLRAGSVSRHAWDHAQDGPMRSQVNTVAEQGELGDHFARLLDDYFIEAPHIADDREDF 308 Query: 329 LRLGMA 334 ++ A Sbjct: 309 HQIATA 314 >gi|300309621|ref|YP_003773713.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 912 Score = 121 bits (304), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 92/298 (30%), Positives = 138/298 (46%), Gaps = 10/298 (3%) Query: 27 VLSQQHRPLRLRWRAQETHFAQWLLVQRVDIEEACLEGMQAKLTCVSSDPELPLQALLGQ 86 +L+ + R LRL + Q L+ +V EA + ++ C SS +L + AL G Sbjct: 20 LLTHERRLLRLHLPSSAPSAVQRLVALQVTGTEAVCGNVIYRVQCASSAADLDIHALQGV 79 Query: 87 PLGIEMVTDRGGLHHTNGIITEARAGQADGALRVYQLTLKDALSIMEERINTRVFRQLSV 146 P+G + GG G+ T R ++ + ++ L DAL ++E+R RVF LSV Sbjct: 80 PIGFSVKEGDGGAALVCGLATSVRQIHSEAGAVLLEIELCDALRLLEQRKTWRVFCDLSV 139 Query: 147 PRILEIMLGEWRQRSPALATAFHFDLDGLEDRRYPVREISHQFNESDAHFIRRLCRREGI 206 I E +L E R + LA A + + GL + YP R Q ESDA F++RL R+EGI Sbjct: 140 LEISEQILTEHRHDNSVLAAALRWHVSGLH-KAYPKRAFVMQAGESDADFLQRLWRQEGI 198 Query: 207 AWYVASPQPTAETGRTEDADVPMHALVFCDQSALLPRALAGTVRYHRDGATEERDTISAW 266 AW+ + + P+H L D + P A +R+H+ G E+ DT++ W Sbjct: 199 AWHFRF---------ALEGNEPVHHLHLADHPSAYPDHSAVALRFHQAGVMEQTDTVTHW 249 Query: 267 SRQRSLVPGRVWRGSWDYKAVRVEEAASHSAIDQGDTGRALAAILNDGLIEAPHAADG 324 V ++DYKA R +A + + G ALA L D P A G Sbjct: 250 EAWCEQSIDEVTLTAFDYKANRCTQAQEKALRGKDGAGLALARTLEDYEYAPPVLAAG 307 >gi|300310083|ref|YP_003774175.1| VGR-related type VI secretion system protein [Herbaspirillum seropedicae SmR1] Length = 944 Score = 100 bits (250), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 75/281 (26%), Positives = 131/281 (46%), Gaps = 22/281 (7%) Query: 29 SQQHRPLRLRWRAQETHFAQWLLVQRVDIEEAC------------LEGMQAKLTCVSSDP 76 SQ R +RL + + L+ +R+ E + G + +L+C+S + Sbjct: 14 SQSARVMRLHTPLDQDYGPHALMAERLQAVEQVGPGEDSSAIDQAIAGYRLQLSCLSLNA 73 Query: 77 ELPLQALLGQPLGIEMVTDRGGLHHTNGIITEARAGQADGALRVYQLTLKDALSIMEERI 136 ++ L+ LLGQP+ +E+ TD GG +G +T R A+G + Y LT++ LS + R Sbjct: 74 DISLRKLLGQPVLLELDTD-GGARAFHGHVTACRMEGANGGMARYLLTIEPWLSFLRHRR 132 Query: 137 NTRVFRQLSVPRILEIMLGEWRQRSPALATAFHFDLDGLEDRRYPVREISHQFNESDAHF 196 ++ VF+ +S P I+E + ++R + L A+ DL + Y R ++ Q+ E+D F Sbjct: 133 DSAVFQDMSAPAIIEAVFADYRGQG-RLQPAWRLDLQ--DASAYAKRSLATQYQETDLAF 189 Query: 197 IRRLCRREGIAWYVASPQPTAETGRTEDADVPMHALVFCDQSALLPRALAGTVRYHRDGA 256 + RL EG+ +Y+ G + A + H +V D + TV + + A Sbjct: 190 VTRLMVEEGLFYYI------EHQGDSNSASLGSHTVVIADHNGSFKPNARSTVAFAQTNA 243 Query: 257 TEERDTISAWSRQRSLVPGRVWRGSWDYKAVRVEEAASHSA 297 +D I W R V +WDY+ V A++ S+ Sbjct: 244 VMAQDNIDRWRSVRRWQTNAVEILTWDYRQTAVRAASTASS 284 >gi|300310071|ref|YP_003774163.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 954 Score = 90.5 bits (223), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 73/293 (24%), Positives = 129/293 (44%), Gaps = 13/293 (4%) Query: 12 MTDQFQTIAAAFAGAVLSQQHRPLRLRWRAQETHFAQWLLVQRVDIEEACLEGMQAKLTC 71 + D G LSQ R + + AQ + L V+R + EE+ + + + Sbjct: 5 LPDSLTAAVTELFGTGLSQNARLITMT-TAQSAGLPETLAVERFEGEESVNDNFRFDIDA 63 Query: 72 VSSDPELPLQALLGQPLGIEMVTDRGGLHHTNGIITEARAGQADGALRVYQLTLKDALSI 131 +S +L L+ +G + + ++ GG +G T+A ADG L Y+L L+ L+ Sbjct: 64 LSISTDLDLKQFIGAEISLRLLQADGGTRAWHGYCTQASWLGADGGLARYRLRLESFLAF 123 Query: 132 MEERINTRVFRQLSVPRILEIMLGEWRQRSPALATAFHFDLDGLEDRRYPVREISHQFNE 191 ++ R ++ +F+ ++V I +L E+ Q + +L + R+I Q+ E Sbjct: 124 LDRRHDSFLFQDMTVTDIAAAVLKEYPQANFSLEVT----------QTLAKRDICTQYRE 173 Query: 192 SDAHFIRRLCRREGIAWYVASPQPTAETGRTEDADVPMHALVFCDQSALLPRALAGT--V 249 +D F+RR+ EG+ + Q A + H LV D++A P G + Sbjct: 174 TDYAFLRRILASEGLNFRFEHEQDDATQQHDQSQPHARHKLVIFDRNAKAPALPGGDEYI 233 Query: 250 RYHRDGATEERDTISAWSRQRSLVPGRVWRGSWDYKAVRVEEAASHSAIDQGD 302 R+HR A E D I+ ++ RS+ P V SW + + S +D G+ Sbjct: 234 RFHRVAAMEASDAITDFAAVRSVRPNGVALASWHPEKLSSPSHEEGSTLDAGE 286 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.133 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,217,554 Number of extensions: 49481 Number of successful extensions: 151 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 139 Number of HSP's successfully gapped: 8 Length of query: 375 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 288 Effective length of database: 1,207,925 Effective search space: 347882400 Effective search space used: 347882400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)