BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0185.p (254 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspiri... 473 e-135 gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 137 1e-33 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 114 9e-27 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 110 1e-25 gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 110 2e-25 >gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 473 bits (1216), Expect = e-135, Method: Compositional matrix adjust. Identities = 242/254 (95%), Positives = 250/254 (98%) Query: 1 MDIQLTKADGILTIHFDREDKKNAITAAMYQQLADALKEAETDNSVRAILFTGKPKSFSA 60 MDIQL+KADG+LTIHFDREDKKNAITAAMYQQLADALKEAE+D++VRAILFTGKPK+FSA Sbjct: 3 MDIQLSKADGVLTIHFDREDKKNAITAAMYQQLADALKEAESDSAVRAILFTGKPKAFSA 62 Query: 61 GNDLEDFLKNPPKTQDAPVFQFLWQISHASKPIVAAVSGVAVGIGTTLLLHCDLVYAADN 120 GNDLEDFLKNPPKTQDAPVFQFLWQISHASKPIVAAVSGVAVGIGTTLLLHCDLVYAADN Sbjct: 63 GNDLEDFLKNPPKTQDAPVFQFLWQISHASKPIVAAVSGVAVGIGTTLLLHCDLVYAADN 122 Query: 121 AKFSMPFTQLGLCPEAASSLLLPHIAGYQRAAEKLLLGEAFGADEACQMGVVNRVLPLEE 180 AKFSMPFTQLGLCPEAASSLLLPHIAGYQRAAEKLLLGEAFGADEACQMG VNRV+ EE Sbjct: 123 AKFSMPFTQLGLCPEAASSLLLPHIAGYQRAAEKLLLGEAFGADEACQMGFVNRVMAQEE 182 Query: 181 LLPYAQQQAAKLAALPAASIRVTKQLMKKNRVTATEQQMVAEIEHFSRMLTAPEAKEAFT 240 LLPYAQQQAAKLAALPAASIRVTKQLMKKNRVTATEQQMVAEIEHFSRMLTAPEAKEAFT Sbjct: 183 LLPYAQQQAAKLAALPAASIRVTKQLMKKNRVTATEQQMVAEIEHFSRMLTAPEAKEAFT 242 Query: 241 AFFEKRKPDFTQFS 254 AFFEKRKPDF QF+ Sbjct: 243 AFFEKRKPDFKQFA 256 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 137 bits (344), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 85/256 (33%), Positives = 126/256 (49%), Gaps = 7/256 (2%) Query: 3 IQLTKADGILTIHFDREDKKNAITAAMYQQLADALKEAETDNSVRAILFTGKPKSFSAGN 62 I L DGI I +R DK N+ T AM+ +L DA+ + D SVR +L TG + F AG Sbjct: 6 ILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRGFCAGQ 65 Query: 63 DLEDFLKNPP-------KTQDAPVFQFLWQISHASKPIVAAVSGVAVGIGTTLLLHCDLV 115 DL D P ++ D + + P++ AV+GVA G G L L CD+V Sbjct: 66 DLGDRAVKPGDDGVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPLACDIV 125 Query: 116 YAADNAKFSMPFTQLGLCPEAASSLLLPHIAGYQRAAEKLLLGEAFGADEACQMGVVNRV 175 AA +A F F +LGL P+ + LP + G RA LLGE A++A + G++ + Sbjct: 126 LAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWGLIWKC 185 Query: 176 LPLEELLPYAQQQAAKLAALPAASIRVTKQLMKKNRVTATEQQMVAEIEHFSRMLTAPEA 235 + ++L+ A AA A P + TK + + +Q+ E + + + + Sbjct: 186 VDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALGRSRDY 245 Query: 236 KEAFTAFFEKRKPDFT 251 +E TAF EKR P FT Sbjct: 246 REGVTAFLEKRAPQFT 261 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 114 bits (285), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 77/242 (31%), Positives = 123/242 (50%), Gaps = 1/242 (0%) Query: 11 ILTIHFDREDKKNAITAAMYQQLADALKEAETDNSVRAILFTGKPKSFSAGNDLEDFL-K 69 +L + +R +NA+ +++ AL AE D+SVR ++ +G K+F+AG DL + + K Sbjct: 19 VLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVISGNEKAFAAGADLNEMIHK 78 Query: 70 NPPKTQDAPVFQFLWQISHASKPIVAAVSGVAVGIGTTLLLHCDLVYAADNAKFSMPFTQ 129 + TQ Q+ I+ KPI+AAV+G A+G G LL+H D+ AA AK P Sbjct: 79 DAIATQLDVRAQYWRAIARFPKPILAAVNGYALGAGCELLMHADIAIAARGAKIGQPEIN 138 Query: 130 LGLCPEAASSLLLPHIAGYQRAAEKLLLGEAFGADEACQMGVVNRVLPLEELLPYAQQQA 189 +G P A + L G A + +L GE AD+A Q G+V V+ + L A Sbjct: 139 VGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLVAEVVDDDATLERTLALA 198 Query: 190 AKLAALPAASIRVTKQLMKKNRVTATEQQMVAEIEHFSRMLTAPEAKEAFTAFFEKRKPD 249 +A ++R+ K+ M ++ E ++ E + FS M + + +E AF EKR Sbjct: 199 HSIAQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSLMAASADRQEGIAAFQEKRAAV 258 Query: 250 FT 251 F+ Sbjct: 259 FS 260 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 110 bits (274), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 76/258 (29%), Positives = 122/258 (47%), Gaps = 16/258 (6%) Query: 2 DIQLTKADGILTIHFDREDKKNAITAAMYQQLADALKEAETDNSVRAILFTGKPKSFSAG 61 +IQ+ + + I R NA++ + +L +AL + + I+ TG K+F+AG Sbjct: 5 NIQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEKAFAAG 64 Query: 62 NDLE--------DFLKNPPKTQDAPVFQFLWQ-ISHASKPIVAAVSGVAVGIGTTLLLHC 112 D+ D K T++ W+ + KP++AAV+G A+G G L + C Sbjct: 65 ADISAMAGYDYMDVFKGEFITRN-------WETLRRIRKPVIAAVAGYALGGGCELAMMC 117 Query: 113 DLVYAADNAKFSMPFTQLGLCPEAASSLLLPHIAGYQRAAEKLLLGEAFGADEACQMGVV 172 D + AADNAKF P +LG+ P A + LP +A + L G A+EA + G+V Sbjct: 118 DFIIAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLV 177 Query: 173 NRVLPLEELLPYAQQQAAKLAALPAASIRVTKQLMKKNRVTATEQQMVAEIEHFSRMLTA 232 +R++ ++LL A A +A +P + K + + T + M E F Sbjct: 178 SRIVAADKLLEEAMAAAIIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYSCFAT 237 Query: 233 PEAKEAFTAFFEKRKPDF 250 + KE AF EKR+P F Sbjct: 238 EDQKEGMKAFLEKRQPVF 255 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 110 bits (274), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 74/248 (29%), Positives = 125/248 (50%), Gaps = 11/248 (4%) Query: 10 GILTIHFDREDKKNAITAAMYQQLADALKEAETDNSVRAILFTGKPKSFSAGNDLEDFLK 69 GI T+ +R NA++ M L L + ++D++VR ++ K+F AG+DL Sbjct: 16 GITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANGKAFCAGHDLRQMQA 75 Query: 70 NPPKTQDAPVFQFLWQISHASK----PIVAAVSGVAVGIGTTLLLHCDLVYAADNAKFSM 125 P +T +F + + + P++A V G+A G L+ CDL AA++A+F++ Sbjct: 76 TPQQTYYEALFHRCSHLMQSLRELPVPVIAKVQGLATAAGCQLVATCDLAIAAESARFAV 135 Query: 126 PFTQLGL-C--PEAASSLLLPHIAGYQRAAEKLLLGEAFGADEACQMGVVNRVLPLEELL 182 +GL C P A + LP +RA E L+ G+ A A G++N+ +P +EL Sbjct: 136 SGINVGLFCSTPAVALTRNLP----IKRAFEMLVTGKFIDAATAAAWGLINQAVPEQELD 191 Query: 183 PYAQQQAAKLAALPAASIRVTKQLMKKNRVTATEQQMVAEIEHFSRMLTAPEAKEAFTAF 242 Q A ++ + PA SI K ++ + ++ A E + + PEA+E TAF Sbjct: 192 SATQALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAEVMACNIMDPEAQEGITAF 251 Query: 243 FEKRKPDF 250 +KRKP++ Sbjct: 252 LDKRKPNW 259 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.131 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 619,899 Number of extensions: 19747 Number of successful extensions: 83 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 74 Number of HSP's successfully gapped: 8 Length of query: 254 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 171 Effective length of database: 1,226,865 Effective search space: 209793915 Effective search space used: 209793915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)