BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0010.0184.p
         (304 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310486|ref|YP_003774578.1| major facilitator superfamily p...   551   e-158
gi|300310608|ref|YP_003774700.1| major facilitator superfamily p...    74   1e-14

>gi|300310486|ref|YP_003774578.1| major facilitator superfamily
           permease [Herbaspirillum seropedicae SmR1]
          Length = 430

 Score =  551 bits (1419), Expect = e-158,   Method: Compositional matrix adjust.
 Identities = 274/293 (93%), Positives = 284/293 (96%)

Query: 1   VAANTLGATIVSRWFETRRGLAMGLLTASAATGQMVFLPLMASMVGSYGWRSVAFLVSAV 60
           VAANTLGATIVSRWFETRRGLAMGLLTASAATGQMVFLPLMA+MVGSYGWRSVAFLVSAV
Sbjct: 126 VAANTLGATIVSRWFETRRGLAMGLLTASAATGQMVFLPLMAAMVGSYGWRSVAFLVSAV 185

Query: 61  ALLAIPLVWLLLPEGPSAIGQKMVGQTSETADDGRSKRNPLAIALDALRTSVRMPDFWLL 120
           ALLAIPLVWLLLPE P+AIGQKM+GQTSE  D+G S+RNPL IALDALRTSVRMPDFWLL
Sbjct: 186 ALLAIPLVWLLLPESPAAIGQKMLGQTSEIKDEGLSRRNPLFIALDALRTSVRMPDFWLL 245

Query: 121 FMSFFVCGLSTNGYIGTQFIAMCNDYGINEVKGASILAAMGMLDLVGTTLSGWLSDRYNP 180
           F SFFVCGLSTNGYIGTQFIAMCNDYGINEV  ASILAAMGMLDLVGTTLSGWLSDRYNP
Sbjct: 246 FASFFVCGLSTNGYIGTQFIAMCNDYGINEVSSASILAAMGMLDLVGTTLSGWLSDRYNP 305

Query: 181 RVLLFWYYGLRGLALIFLPYAFGLQYYGLAIFAIFYGLDWIATVPPTVRLANDVFGRLAA 240
           RVLLFWYYGLRGLALIFLPYAFGLQYYGL IFAIFYGLDWIATVPPTVRLANDVFGRLAA
Sbjct: 306 RVLLFWYYGLRGLALIFLPYAFGLQYYGLTIFAIFYGLDWIATVPPTVRLANDVFGRLAA 365

Query: 241 PIVFGWIVAGHQLGAATATTVAGYMRATLGNYTLSSIMMGVACLIGAVLVLRI 293
           PIVFGWIVAGHQLGAATATT+AGY+RA+LGNYTLSSI+MGVACL+GAVLVLRI
Sbjct: 366 PIVFGWIVAGHQLGAATATTLAGYLRASLGNYTLSSILMGVACLVGAVLVLRI 418


>gi|300310608|ref|YP_003774700.1| major facilitator superfamily
           permease [Herbaspirillum seropedicae SmR1]
          Length = 407

 Score = 74.3 bits (181), Expect = 1e-14,   Method: Compositional matrix adjust.
 Identities = 82/283 (28%), Positives = 130/283 (45%), Gaps = 30/283 (10%)

Query: 18  RRGLAMGLLTASAATGQMVFLPLMASMVGSYGWRSVAFLVSAVALLAIPLVWLLLPEGPS 77
           RR  AMGL++A  + GQ++ LPL+  +  + G      +++ + L   PL   L   GP 
Sbjct: 135 RRSSAMGLVSAGGSLGQVLMLPLVQGLRATSGMSLALMVLALLVLAVAPLGLALARTGPV 194

Query: 78  AIGQKMVGQTSETADDGR---SKRNPLAIALDALRTSVRMPDFWLLFMSFFVCGLSTNGY 134
           A            AD  R   S R  +A A     T+ R   + LL + FF CG     +
Sbjct: 195 A------------ADAPRLDYSVREVIAQA-----TAHR--GYQLLTLGFFACGFQL-AF 234

Query: 135 IGTQ---FIAMCNDYGINEVKGASILAAMGMLDLVGTTLSGWLSDRYNPRVLLFWYYGLR 191
           I T    ++ +C+   +     A+ LA +G+ ++ G+   GWL  RY P+ +L W Y +R
Sbjct: 235 IATHLPGYLELCH---MPMGLAATALALIGLFNMAGSWACGWLGGRYRPQRVLGWLYLVR 291

Query: 192 GLALIFLPYAFGLQYYGLAIFAIFYGLDWIATVPPTVRLANDVFGRLAAPIVFGWIVAGH 251
             A I L +        + +FA   GL W+ TVP T  L   +FG      +FG     H
Sbjct: 292 STA-IGLFFIAPKSSQSVTLFAAVMGLTWLGTVPLTTGLVARLFGTAHMGTLFGVCFLSH 350

Query: 252 QLGAATATTVAGYMRATLGNYTLSSIMMGVACLIGAVLVLRIK 294
           Q+G+     + G+M    G+Y+L  +   V  ++ A+L   I+
Sbjct: 351 QVGSFLGAWLGGWMLEVTGSYSLLWMATFVVGIVAALLHFPIE 393


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.326    0.140    0.436 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 906,672
Number of extensions: 35821
Number of successful extensions: 222
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 221
Number of HSP's successfully gapped: 2
Length of query: 304
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 219
Effective length of database: 1,217,395
Effective search space: 266609505
Effective search space used: 266609505
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 130 (54.7 bits)