BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0181.p (727 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312322|ref|YP_003776414.1| two component hybrid sensor his... 1144 0.0 gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbas... 197 2e-51 gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 196 4e-51 gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [He... 189 6e-49 gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 187 2e-48 >gi|300312322|ref|YP_003776414.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 775 Score = 1144 bits (2960), Expect = 0.0, Method: Compositional matrix adjust. Identities = 603/715 (84%), Positives = 638/715 (89%), Gaps = 8/715 (1%) Query: 1 MSTPDLSQMSMLDLFRFEAESQIAQLNTSLLALEQQPTAAEHLEACMRAGHSLKGAARIV 60 MSTPDLSQMSMLDLFRFEAESQIAQLN SLLALEQQPTAA+HLEACMRAGHSLKGAARIV Sbjct: 1 MSTPDLSQMSMLDLFRFEAESQIAQLNASLLALEQQPTAADHLEACMRAGHSLKGAARIV 60 Query: 61 GLETAVKVAHVLEDCFVLAQQGKLRLEKKHIDVLLRGADLLGRIASPPDGDETWVDHAGS 120 GLE AVKVAHVLEDCFVLAQQG+L L+KKHIDVLLRGADLL RIA+PPDGDE W DHAGS Sbjct: 61 GLEPAVKVAHVLEDCFVLAQQGRLLLQKKHIDVLLRGADLLARIAAPPDGDEAWADHAGS 120 Query: 121 AEVQAFLQALPAVMSGADADPWPAPSAALQEAFAAEAAP-------QPPAPAPSAAAMEP 173 AEVQAFL ALP+VMSG DA PWPA ++A A A AA Q P P + A P Sbjct: 121 AEVQAFLVALPSVMSGGDAAPWPAATSAATSAPAPAAAEPVVAAAAQLPEPELAEPAPVP 180 Query: 174 PQPAASAPIVNQTAAARAVRVSADSLDRLLSLTGESLVESRRLKPFSASMLRMKRVQREA 233 P A A + N + ARAVRVSADSLDRLL LTGESLVESR+LKPFSASMLRMKR QREA Sbjct: 181 AAPVAGAHLAN-ASGARAVRVSADSLDRLLGLTGESLVESRQLKPFSASMLRMKRTQREA 239 Query: 234 VQALDLLQQKLAASQLDEFAQTTLAELRALMQTNQQLLGEQLIALDAFDRRSVNLSQQLY 293 +QALDLLQQKLA +QLDEFAQT AELR LMQ NQQLLGEQL ALDAFDRRSVNLSQ+LY Sbjct: 240 MQALDLLQQKLATTQLDEFAQTAFAELRTLMQQNQQLLGEQLSALDAFDRRSVNLSQRLY 299 Query: 294 DEALACRMRPFADGTAGFARMVRDVGNALGKSVRLDISGTATQIDRDILEKLEAPLGHLL 353 DEALACRMRPFADGTAG ARMVRDVGN LGKSVRLDI G TQIDRDILEKLEAP+GHLL Sbjct: 300 DEALACRMRPFADGTAGLARMVRDVGNTLGKSVRLDIVGNTTQIDRDILEKLEAPIGHLL 359 Query: 354 RNAVDHGIEDGSTRRAAGKPEQGVVRLEARHSAGMLLIEVADDGAGIDLDALRRTVVMRG 413 RNAVDHGIE + RRAAGKPEQGV+RLEARHSAGMLLIEVADDGAGID++ +R VV RG Sbjct: 360 RNAVDHGIESSAQRRAAGKPEQGVIRLEARHSAGMLLIEVADDGAGIDIEQVRSAVVARG 419 Query: 414 LATQEIAERLSDAELLDFLLLPSFSLRETVTEISGRGVGLDVVADMLKQVRGTIRITTRP 473 L+ QE AERLS+AELL+FLLLPSFSLR+TVTEISGRGVGLDVVADMLKQVRGTIRITT+ Sbjct: 420 LSNQETAERLSEAELLEFLLLPSFSLRDTVTEISGRGVGLDVVADMLKQVRGTIRITTQA 479 Query: 474 GQGSRFLLQLPLTLSVIRSLLVEIAGEPYAFPLAYVNRTLRLPAEALQTLEGYQHFSHGG 533 GQGSRFL+QLPLTLSVIRSLLV+IAGEPYAFPLAYVNRTLRLPAEALQTLEGYQHFSHGG Sbjct: 480 GQGSRFLMQLPLTLSVIRSLLVDIAGEPYAFPLAYVNRTLRLPAEALQTLEGYQHFSHGG 539 Query: 534 RQVGLVSAHQVLQRGQWRVRDGSVCVVVIGEEEHSYGIAVDAFLGERMLVVQPLDARLGK 593 RQVGLVSAHQVLQ+GQWR RDGSVCVVVIGE+EH+YG+AVDAFLGERMLVVQPLD RLGK Sbjct: 540 RQVGLVSAHQVLQKGQWRSRDGSVCVVVIGEQEHTYGLAVDAFLGERMLVVQPLDPRLGK 599 Query: 594 IPDILAGALMEDGAPLLILDVADLLRTVEKLTASGRLQTVDADPAGAAAVAVRKKVLVVD 653 I D+LAGALMEDG PLLILDVAD+LRTVEKLT+SGRLQTVD D A A RKKVLVVD Sbjct: 600 IADVLAGALMEDGTPLLILDVADMLRTVEKLTSSGRLQTVDQDGAAPTTTAARKKVLVVD 659 Query: 654 DSLTVRELERKLLTNRGYQVTVAVDGMDGWNAVRAEHFDLVITDIDMPRMDGIEL 708 DSLTVRELERKLLTNRGYQVTVAVDGMDGWNAVRA+ FDLVITDIDMPRMDGIEL Sbjct: 660 DSLTVRELERKLLTNRGYQVTVAVDGMDGWNAVRADSFDLVITDIDMPRMDGIEL 714 >gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbaspirillum seropedicae SmR1] Length = 638 Score = 197 bits (502), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 145/423 (34%), Positives = 213/423 (50%), Gaps = 57/423 (13%) Query: 175 QPAASAPIVNQTAAA--------RAVRVSADSLDRLLSLTGESLVESRRLKPFSASMLRM 226 +P A+AP + A+ ++R+ A LD LL + GES+ Sbjct: 226 EPKAAAPAQHNRRASDSPGGAKEESIRIDAGKLDALLEVAGESV---------------- 269 Query: 227 KRVQREAVQALDLLQQKLAASQLDEFAQTTLAELRALMQTNQQLLGEQLIALDAFDRRSV 286 + +A L+ LQQ +F T ALM T + LG R+ Sbjct: 270 -QAANQAAVLLEKLQQF-------KFEGTA----AALMSTLAETLG-----------RAS 306 Query: 287 NLSQQLYDEALACRMRPFADGTAGFARMVRDVGNALGKSVRLDISGTATQIDRDILEKLE 346 S +L L+ RM+P F R+VR++ LGK V L I+G T++DR +++ L Sbjct: 307 RYSSELQRATLSTRMQPVGRLFQKFPRLVRELAKDLGKDVDLVITGAETEVDRVVVDSLY 366 Query: 347 APLGHLLRNAVDHGIEDGSTRRAAGKPEQGVVRLEARHSAGMLLIEVADDGAGIDLDALR 406 PL H+LRN++DHGIE R AAGKP + + L A + ++IEV DDG G+D D LR Sbjct: 367 DPLVHMLRNSLDHGIE--VDRVAAGKPTKATISLNAWQAGSSVMIEVFDDGKGMDPDMLR 424 Query: 407 RTVVMRGLATQEIAERLSDAELLDFLLLPSFSLRETVTEISGRGVGLDVVADMLKQVRGT 466 + +GL + A L+ E L + LP FS +E + +SGRGVG+DVV +++ RGT Sbjct: 425 SKALAKGLINEHQA--LTADEALQLVFLPGFSTKEVASSVSGRGVGMDVVKTAVEKHRGT 482 Query: 467 IRITTRPGQGSRFLLQLPLTLSVIRSLLVEIAGEPYAFPLAYVNRTLRLPAEALQTLEGY 526 IRI + G+G++F ++LP+ LS+I ++LV AG P A P+A V R + LP E + G Sbjct: 483 IRIESTIGKGTKFSIRLPIELSIIPTMLVRCAGAPLALPMAVVERVVELP-ENFDEVGGA 541 Query: 527 QHFSHGGRQVGLVSAHQVLQRGQWRVRDGSVCVVVIGEEEHSYGIAVDAFLGERMLVVQP 586 GR + + S VL R G V V H Y + V++ G LV++P Sbjct: 542 PVLRDQGRPLPVNSLAGVLGYEPGNERVGIVMAV-----PHPYILGVESVEGTADLVIKP 596 Query: 587 LDA 589 L A Sbjct: 597 LTA 599 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 196 bits (499), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 113/338 (33%), Positives = 186/338 (55%), Gaps = 4/338 (1%) Query: 289 SQQLYDEALACRMRPFADGTAGFARMVRDVGNALGKSVRLDISGTATQIDRDILEKLEAP 348 ++ L + ++ RM P + F RMVRD+ + LGK V G AT++D+ ++E++ P Sbjct: 357 TRDLQESVMSIRMMPMDYVFSRFPRMVRDLAHKLGKQVEFVTHGAATELDKGLIERIVDP 416 Query: 349 LGHLLRNAVDHGIEDGSTRRAAGKPEQGVVRLEARHSAGMLLIEVADDGAGIDLDALRRT 408 L HL+RN++DHGIE RRA+GK E G + L A H G ++IEV DDG G++ + + Sbjct: 417 LTHLVRNSIDHGIELPDARRASGKSEAGTLSLSAEHQGGNIIIEVTDDGGGLNREKILAK 476 Query: 409 VVMRGLATQEIAERLSDAELLDFLLLPSFSLRETVTEISGRGVGLDVVADMLKQVRGTIR 468 +G+ + + LSDAE+ + P FS E VT++SGRGVG+DVV + + G + Sbjct: 477 AKQQGMP---VTDNLSDAEVWQLIFEPGFSTAEQVTDVSGRGVGMDVVKRNILAMGGAVD 533 Query: 469 ITTRPGQGSRFLLQLPLTLSVIRSLLVEIAGEPYAFPLAYVNRTLRLPAEALQTLEGY-Q 527 I + G G+ + LPLTL+++ + + I E Y PL YV +L+ A+ ++ + G Q Sbjct: 534 IRSSKGFGTTISISLPLTLAILDGMSIRIGEEVYILPLGYVVESLQPLAQDIKEISGQGQ 593 Query: 528 HFSHGGRQVGLVSAHQVLQRGQWRVRDGSVCVVVIGEEEHSYGIAVDAFLGERMLVVQPL 587 G + L+ +Q+ VV++ + + VD +G++ +VV+ L Sbjct: 594 VLRVRGEYLPLIPLYQIFNIEPSFTDPSQGLVVILESDGKKAALFVDDLIGQQQIVVKNL 653 Query: 588 DARLGKIPDILAGALMEDGAPLLILDVADLLRTVEKLT 625 ++ K+ I ++ DG LI+DVA LLR+ +L Sbjct: 654 ESNYRKVAGISGATILGDGGVALIVDVAALLRSSRQLN 691 >gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 837 Score = 189 bits (480), Expect = 6e-49, Method: Compositional matrix adjust. Identities = 108/333 (32%), Positives = 189/333 (56%), Gaps = 2/333 (0%) Query: 288 LSQQLYDEALACRMRPFADGTAGFARMVRDVGNALGKSVRLDISGTATQIDRDILEKLEA 347 +++++ D + RM P + F R+VRD+ LGK V+L + G T+ D++I+E L Sbjct: 505 IAEEMQDAIMQVRMMPVSVVFQRFPRLVRDISRKLGKEVQLVLVGEETEADKNIIEALAD 564 Query: 348 PLGHLLRNAVDHGIEDGSTRRAAGKPEQGVVRLEARHSAGMLLIEVADDGAGIDLDALRR 407 PL H++RN++DHG+E RRA+GKP G + + A H ++I + DDG GID ++R Sbjct: 565 PLIHIVRNSLDHGLEGPEQRRASGKPALGSLTISAAHEGDQVVIVIEDDGKGIDPALIKR 624 Query: 408 TVVMRGLATQEIAERLSDAELLDFLLLPSFSLRETVTEISGRGVGLDVVADMLKQVRGTI 467 +GL + ER+SD + ++ + FS E V+++SGRGVG+DVV + +++V G++ Sbjct: 625 KAYEKGLIDEAQLERISDQDAVNLVFSSGFSTAEVVSDLSGRGVGMDVVRNAVEKVNGSL 684 Query: 468 RITTRPGQGSRFLLQLPLTLSVIRSLLVEIAGEPYAFPLAYVNRTLRLPAEALQTLEGYQ 527 + + G+G+R + LPL+++V + +++E G+ + P+ +V T+R+P +L Sbjct: 685 ALHSVSGKGTRLQISLPLSMAVTKVMIIESDGQLFGVPMEHVMETVRVPRSSLHFANSSA 744 Query: 528 HFSHGGRQVGLVSAHQVLQRG--QWRVRDGSVCVVVIGEEEHSYGIAVDAFLGERMLVVQ 585 G +GL +Q+L Q DG V V+V+ S G+ +D F ++ + Sbjct: 745 KMVLRGHILGLKGINQILGIAAEQKTNADGEVAVLVVRVGGQSVGLVIDDFRETVDVIQK 804 Query: 586 PLDARLGKIPDILAGALMEDGAPLLILDVADLL 618 PL LG + ALM DG+ L++L+ ++ Sbjct: 805 PLLGILGGLSLYSGSALMGDGSVLMVLNTREMF 837 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 187 bits (476), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 117/337 (34%), Positives = 183/337 (54%), Gaps = 8/337 (2%) Query: 287 NLSQQLYDEALACRMRPFADGTAGFARMVRDVGNALGKSVRLDISGTATQIDRDILEKLE 346 L +Q+ D AL RM + F R+VRD+ LGK + L ++G+ T++D+ ++E+L Sbjct: 379 TLMEQIRDAALTLRMVQINEVFQRFPRIVRDIARDLGKDIDLVMTGSDTELDKSMVERLA 438 Query: 347 APLGHLLRNAVDHGIEDGSTRRAAGKPEQGVVRLEARHSAGMLLIEVADDGAGIDLDALR 406 PL H++RNA+DHGIE + R AAGKP + ++L A H +G ++IEV DDG G+D +A+ Sbjct: 439 DPLMHIVRNAIDHGIEPAAERLAAGKPAKASLQLNAIHESGSVVIEVMDDGRGMDREAIL 498 Query: 407 RTVVMRGLATQEIAERLSDAELLDFLLLPSFSLRETVTEISGRGVGLDVVADMLKQVRGT 466 V +GLA+ + LSDAE+ F+ P FS VTE+SGRGVG+DVV ++ + G Sbjct: 499 AKAVAQGLASPD--AELSDAEIYRFVFEPGFSTARQVTELSGRGVGMDVVRRNIEALHGE 556 Query: 467 IRITTRPGQGSRFLLQLPLTLSVIRSLLVEIAGEPYAFPLAYVNRTLRLPAEALQTLEGY 526 + I T G+G+ L+LPLTL++I V + + PL V + +P + G Sbjct: 557 VSIDTGRGRGTVVRLRLPLTLAIISGFQVVVGNAVFVIPLDMVVECIDMPPQQ----PGS 612 Query: 527 QHFSHGGRQVGLVSAHQVLQRGQWRVRDGSVCVVVIGEEEHSYGIAVDAFLGERMLVVQP 586 + + V ++ S+ +V G G+ VD LGE V++P Sbjct: 613 HIVNVRDEPLPFVPLRELFDLPARERGRNSLVIVQYGPLR--AGLLVDGLLGECQAVIKP 670 Query: 587 LDARLGKIPDILAGALMEDGAPLLILDVADLLRTVEK 623 L GK+ + ++ DG LILD+A L+ ++ Sbjct: 671 LGRLFGKVKGLSGSTILGDGRVALILDIAHLVHHTQQ 707 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,808,352 Number of extensions: 70323 Number of successful extensions: 414 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 411 Number of HSP's successfully gapped: 9 Length of query: 727 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 635 Effective length of database: 1,184,250 Effective search space: 751998750 Effective search space used: 751998750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)