BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0178.p (156 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312321|ref|YP_003776413.1| protein-glutamate methylesteras... 257 3e-70 gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylester... 95 3e-21 gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylester... 89 2e-19 gi|300311029|ref|YP_003775121.1| protein-glutamate methylesteras... 78 3e-16 >gi|300312321|ref|YP_003776413.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 257 bits (657), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 125/153 (81%), Positives = 134/153 (87%) Query: 1 VIVQHIDAAFAPGMAQWLQQQSVLRVRLAVEGDLPRRGEVLLAGSNHHLVFKEGSRLAYV 60 VIVQHIDAAFAPGMAQWLQQQS L+V LA EG P+RG VLLA SN HLV KEG RL YV Sbjct: 189 VIVQHIDAAFAPGMAQWLQQQSALKVGLAQEGQAPQRGAVLLAASNRHLVVKEGGRLGYV 248 Query: 61 EGAPTDVYRPSVDAFFHSIVRHWDGKATGVLLTGMGRDGAAGLKALRDNGYHTLAQDQKS 120 EGAP DVYRPSVD FF SIVRHW GK G+LLTGMGRDGAAGLKALRD+G HT+AQDQKS Sbjct: 249 EGAPNDVYRPSVDVFFQSIVRHWPGKVAGLLLTGMGRDGAAGLKALRDSGAHTMAQDQKS 308 Query: 121 CAVYGMPKAAVALNAAVDVLPVSAMAARLQALL 153 CAVYGMPKAAVALNAAV+VLPV+ MAARLQA++ Sbjct: 309 CAVYGMPKAAVALNAAVEVLPVTGMAARLQAII 341 >gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 95.1 bits (235), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 57/157 (36%), Positives = 84/157 (53%), Gaps = 3/157 (1%) Query: 1 VIVQHIDAAFAPGMAQWLQQQSVLRVRLAVEGDLPRRGEVLLAGSNHHL-VFKEGSRLAY 59 +I QH+ F AQ L + V + + G +A + HL + + G+ Sbjct: 194 LITQHMPEGFTRSFAQRLNNLCKISVVESAGNERVLPGHAYIAPGHSHLKLVRSGANYMT 253 Query: 60 V--EGAPTDVYRPSVDAFFHSIVRHWDGKATGVLLTGMGRDGAAGLKALRDNGYHTLAQD 117 +G P + +RPSVD F S A GV+LTGMG+DGA G+ +R+ G + AQD Sbjct: 254 QLDQGPPVNRHRPSVDVLFQSAATCAGKNAVGVILTGMGKDGAQGMLEMRNAGAYNFAQD 313 Query: 118 QKSCAVYGMPKAAVALNAAVDVLPVSAMAARLQALLA 154 + SC V+GMP+ A+A+ AA +VL + AM R+ A LA Sbjct: 314 EASCVVFGMPREAIAVGAAHEVLALQAMPGRVLAWLA 350 >gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 381 Score = 88.6 bits (218), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 55/153 (35%), Positives = 80/153 (52%), Gaps = 3/153 (1%) Query: 1 VIVQHIDAAFAPGMAQWLQQQSVLRVRLAVEGDLPRRGEVLLAGSNHHLVFKEGSRLAYV 60 V+VQH+ F A+ L + S V+ A D G VL+A HLV + YV Sbjct: 224 VVVQHMPQRFTRSFAERLHRISGAEVKEAEHLDRILPGRVLIAPGGRHLVVRREGLQYYV 283 Query: 61 E---GAPTDVYRPSVDAFFHSIVRHWDGKATGVLLTGMGRDGAAGLKALRDNGYHTLAQD 117 E G ++PSVD F S+ + A G+++TGMG DGA G++ L D G T AQD Sbjct: 284 ETLDGPLVSRHKPSVDVLFRSLAKAAGSNAVGIIMTGMGDDGARGMRELADCGGATYAQD 343 Query: 118 QKSCAVYGMPKAAVALNAAVDVLPVSAMAARLQ 150 + S V+GMPK A+ DV+ + ++A ++ Sbjct: 344 EASSVVFGMPKEAIQQGGVGDVITLEHISAVIE 376 >gi|300311029|ref|YP_003775121.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 78.2 bits (191), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 53/157 (33%), Positives = 82/157 (52%), Gaps = 4/157 (2%) Query: 1 VIVQHIDAAFAPGMAQWLQQQSVLRVRLAVEGDLPRRGEVLLA-GSNHHLVFKEGSRLAY 59 ++ QH+ AAF A+ + + L V + + G V +A G LV K GS L+ Sbjct: 195 LVAQHMPAAFTRSFAERMDKLCALNVLEVAAPTVVQGGHVYIARGGADMLVAKRGSHLSV 254 Query: 60 VEG--APTDVYRPSVDAFFHSIVRHWDGKA-TGVLLTGMGRDGAAGLKALRDNGYHTLAQ 116 + +PT ++ PSV+ S ++ + GV+LTGMG DGAA + +G T+A+ Sbjct: 255 LPKPESPTHLWHPSVELLARSALQSMAARQLIGVMLTGMGNDGAAAFAEICGDGGRTIAE 314 Query: 117 DQKSCAVYGMPKAAVALNAAVDVLPVSAMAARLQALL 153 + SC V+GMPK + A VLP S +A +L+ L Sbjct: 315 SESSCVVFGMPKDLIDRGGASVVLPASKIATQLRVWL 351 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 484,205 Number of extensions: 20330 Number of successful extensions: 70 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 68 Number of HSP's successfully gapped: 5 Length of query: 156 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 78 Effective length of database: 1,250,540 Effective search space: 97542120 Effective search space used: 97542120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)