BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0172.p (230 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herb... 469 e-134 gi|300311872|ref|YP_003775964.1| glutathione S-transferase famil... 225 2e-60 gi|300310406|ref|YP_003774498.1| glutathione S-transferase [Herb... 70 2e-13 gi|300311017|ref|YP_003775109.1| glutathione S-transferase famil... 67 1e-12 >gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 230 Score = 469 bits (1207), Expect = e-134, Method: Compositional matrix adjust. Identities = 222/230 (96%), Positives = 227/230 (98%) Query: 1 MIDVYSWATPNGHKVHIMLEECGLPYTAHAIDIGAGDQFTPEFLKISPNNKIPAIVDPDG 60 MIDVYSWATPNGHKVHIMLEECGLPYTAHAIDIGAGDQFTPEFLKISPNNKIPAIVDPDG Sbjct: 1 MIDVYSWATPNGHKVHIMLEECGLPYTAHAIDIGAGDQFTPEFLKISPNNKIPAIVDPDG 60 Query: 61 PDGAPISLFESGAILIYLAGKTGRFLGETVREKYEVLQWVMFQMGGLGPMLGQAHHFRLY 120 PDG PISLFESGAILIYLAGKTGRFLGET REKYEVLQWVMFQMGGLGPMLGQAHHFRLY Sbjct: 61 PDGEPISLFESGAILIYLAGKTGRFLGETTREKYEVLQWVMFQMGGLGPMLGQAHHFRLY 120 Query: 121 APEQIEYAVNRYSNEARRLYQVMDKQLGQHAYLAGENYTIADIACFPWTRSHKNQGIDLA 180 APEQIEYAVNRY+NEARRLYQVMDKQLGQHAYLAGE+YTIADIACFPWTRSHKNQGIDL Sbjct: 121 APEQIEYAVNRYTNEARRLYQVMDKQLGQHAYLAGEHYTIADIACFPWTRSHKNQGIDLD 180 Query: 181 DFPNVQRWFDAISARPAVQRGVTVLADKRKPLHDDKAKELLFGKSQYEKR 230 DFPNV+RW+DAISARPAVQRGVTVLADKRKPLHDDKAKELLFGKSQY+KR Sbjct: 181 DFPNVKRWYDAISARPAVQRGVTVLADKRKPLHDDKAKELLFGKSQYQKR 230 >gi|300311872|ref|YP_003775964.1| glutathione S-transferase family protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 225 bits (574), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 113/212 (53%), Positives = 143/212 (67%), Gaps = 7/212 (3%) Query: 2 IDVYSWATPNGHKVHIMLEECGLPYTAHAIDIGAGDQFTPEFLKISPNNKIPAIVDPDGP 61 I +YS TPNG KV IMLEE GLPY AH + DQ +PEFL ++PNNKIPAI+DP+GP Sbjct: 22 IQLYSLPTPNGVKVSIMLEETGLPYEAHLVSFETNDQMSPEFLSLNPNNKIPAIIDPNGP 81 Query: 62 DGAPISLFESGAILIYLAGKTGRFLGETVREKYEVLQWVMFQMGGLGPMLGQAHHFRLYA 121 G P+ LFESGAIL+YLA KTG+FL +Y+V+QWVMFQMGG+GPM GQ F +A Sbjct: 82 GGQPLPLFESGAILLYLAEKTGQFLPADAAARYQVIQWVMFQMGGVGPMFGQLGFFHRFA 141 Query: 122 PEQIE--YAVNRYSNEARRLYQVMDKQLGQHAYLAGENYTIADIACFPWTRS-----HKN 174 ++ E +RY NE++RL VMDK+L Q +L G+ Y+IADIA FPW R+ Sbjct: 142 GKEYEDKRPRDRYVNESKRLLGVMDKRLAQSQWLGGDAYSIADIATFPWIRNLIGFYEAA 201 Query: 175 QGIDLADFPNVQRWFDAISARPAVQRGVTVLA 206 + DFP+V R +A ARPAV RG+ + A Sbjct: 202 DLVGFQDFPHVARALEAFVARPAVVRGLNIPA 233 >gi|300310406|ref|YP_003774498.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 206 Score = 70.1 bits (170), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 51/190 (26%), Positives = 84/190 (44%), Gaps = 15/190 (7%) Query: 11 NGHKVHIMLEECGLPYTAHAIDIGAGDQFTPEFLKISPNNKIPAIVDPDGPDGAPISLFE 70 + H+ + GLP +D+ AG P FL ++P ++P + D + + + + Sbjct: 11 HAHRAVLFASLLGLPAELVEVDLAAGAHKKPAFLALNPFGQVPVLEDGE------VVIAD 64 Query: 71 SGAILIYLAGKTGR--FLGETVREKYEVLQWVMFQMGGL--GPMLGQAHHFRLYAPEQIE 126 S AIL+YLA K GR +L E + V +W+ G L GP RL Sbjct: 65 SNAILVYLAKKAGRSDWLPEDAQGAAAVQRWLSVAAGELAYGPAAA-----RLVTVFGAA 119 Query: 127 YAVNRYSNEARRLYQVMDKQLGQHAYLAGENYTIADIACFPWTRSHKNQGIDLADFPNVQ 186 + A L +++ L + +LA ++ TIAD+A + + +DL +P VQ Sbjct: 120 FNAEEVIQRAHLLLGRLEQHLAKRDWLAADHPTIADVAVYSYLARAPEGNVDLRGYPGVQ 179 Query: 187 RWFDAISARP 196 + I A P Sbjct: 180 GFLQRIEALP 189 >gi|300311017|ref|YP_003775109.1| glutathione S-transferase family protein [Herbaspirillum seropedicae SmR1] Length = 223 Score = 67.4 bits (163), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 62/232 (26%), Positives = 95/232 (40%), Gaps = 49/232 (21%) Query: 1 MIDVYSWATPNGHKVHIMLEECGLPYTAHAIDIGAGDQFTP-EFLKISPNNKIPAIVDPD 59 MI V+ +V +LEE GL Y P E LK+ P K P I D + Sbjct: 1 MITVHHLNNSRSQRVLWLLEELGLDYEIKFYQRDPKTMLAPPELLKVHPLGKSPVITDGE 60 Query: 60 GPDGAPISLFESGAILIYLAGKTGRFLG--------ETVREKY-------EVLQWVMFQM 104 +++ ESG I+ YL + G G E +R +Y + W++ Q+ Sbjct: 61 ------LTVAESGNIIEYLLERYGAHSGLLPASGTPERLRYRYWLHFAEGSAMPWLVMQL 114 Query: 105 -------------GGLGPMLGQAHHFRLYAPEQIEYAVNRYSNEARRLYQVMDKQLGQHA 151 + ML + R+ AP+Q R + M+ +LGQ Sbjct: 115 IFAKMPKAVPTLIRPIARMLAEGMRSRVVAPQQ------------ERQLRFMEDELGQQG 162 Query: 152 YLAGENYTIADI-ACFPWTRSHKNQGIDLADFPNVQRWFDAISARPAVQRGV 202 + AG ++ ADI FP G+D +PN+ ++ +AI ARPA QR + Sbjct: 163 WFAGAQFSGADIQMSFPVEALRARGGLD-GRYPNLLKYLEAIHARPAYQRAL 213 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 815,705 Number of extensions: 36293 Number of successful extensions: 97 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 93 Number of HSP's successfully gapped: 4 Length of query: 230 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 148 Effective length of database: 1,231,600 Effective search space: 182276800 Effective search space used: 182276800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)