BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0171.p (385 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent a... 753 0.0 gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic ami... 116 3e-27 gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic ami... 109 3e-25 gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotr... 88 2e-18 gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Her... 87 3e-18 >gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 753 bits (1943), Expect = 0.0, Method: Compositional matrix adjust. Identities = 362/385 (94%), Positives = 374/385 (97%) Query: 1 MSTPNIVTKLPKVGTTIFTVMSALASEKGAVNLGQGFPDFHCDPALVDAVTRAMQDGLNQ 60 MSTPNIVTKLPKVGTTIFTVMSALASEKGAVNLGQGFPDFHCDPALV AVT+AMQ+GLNQ Sbjct: 1 MSTPNIVTKLPKVGTTIFTVMSALASEKGAVNLGQGFPDFHCDPALVAAVTQAMQEGLNQ 60 Query: 61 YPPMAGVLPLREAIADKVERLYGHRYDPVSEITVTAGATQGILTSVLCAVHPGDEVIVIE 120 YPPMAGV LREAIA+KVE+LYGHRYDPVSEITVTAGATQGILTSVLCAVHPGDEVIVIE Sbjct: 61 YPPMAGVPVLREAIAEKVEKLYGHRYDPVSEITVTAGATQGILTSVLCAVHPGDEVIVIE 120 Query: 121 PVYDCYVPAIELAGGVPVFVQMEVGAAGYSIPWDKVRAAVTPKTRMIMVNTPHNPTGSVM 180 PVYDCYVPAIELAGGVPVFVQMEVGA GYSIPWDKV+AAVTPKTRMIMVNTPHNPTGSVM Sbjct: 121 PVYDCYVPAIELAGGVPVFVQMEVGAQGYSIPWDKVKAAVTPKTRMIMVNTPHNPTGSVM 180 Query: 181 RASDVAALADIVRGTDILILSDEVYEHMVFDGQPHESLARHPELAERSFINSSFGKTYHV 240 RA+DVAALADIVRGTDILILSDEVYEHMV+DGQPHESLARHPELAERSFINSSFGKTYHV Sbjct: 181 RAADVAALADIVRGTDILILSDEVYEHMVYDGQPHESLARHPELAERSFINSSFGKTYHV 240 Query: 241 TGWKVGYVAAPAALTAEFRKVHQFNVFTVNTPVQYGLAEYMKNPAPYLDLPAFYQRKRDL 300 TGWKVGYVAAPAALTAEFRKVHQFNVFTVNTPVQYGLA YMK+PAPYLDLPAFYQ KRDL Sbjct: 241 TGWKVGYVAAPAALTAEFRKVHQFNVFTVNTPVQYGLAAYMKDPAPYLDLPAFYQHKRDL 300 Query: 301 FRTGLANTRFELLPSQGTYFQCVKYDAISDQSEGDFCKWLTSEIGVAAIPVSAFYNTPKE 360 FRTGLANTRFELLPSQGTYFQCVKY AIS E +FCKWLTSEIGVAAIPVSAFYNTP+E Sbjct: 301 FRTGLANTRFELLPSQGTYFQCVKYGAISALPEAEFCKWLTSEIGVAAIPVSAFYNTPRE 360 Query: 361 SGIVRFCFAKKEETLQLALDRLAKL 385 SGIVRFCFAKK+ETLQLAL+RLAKL Sbjct: 361 SGIVRFCFAKKDETLQLALERLAKL 385 >gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 393 Score = 116 bits (291), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 100/363 (27%), Positives = 175/363 (48%), Gaps = 27/363 (7%) Query: 31 VNLGQGFPDFHCDPALVDAVTRAMQDGLNQYPPMAGVLPLREAIADKVERLYGHRYDPVS 90 +++G G PDF PA+V+A TRAM +G QY G+ LR AI+D +YG P Sbjct: 36 IHMGIGEPDFTAPPAVVEAATRAMTEGKMQYTSATGLPQLRAAISDHYRSVYGLEIAP-E 94 Query: 91 EITVTAGATQGILTSVLCAVHPGDEVIVIEPVYDC---YVPAIELAGGVPVFVQMEVGAA 147 I VTAGA+ +L + V EV++ +P Y C +V A E + ++ A+ Sbjct: 95 RIVVTAGASAALLLACAALVERDSEVLMPDPSYPCNRHFVAAFE--------GRAKLIAS 146 Query: 148 G----YSIPWDKVRAAVTPKTRMIMVNTPHNPTGSVMRASDVAALADIVRGTDILILSDE 203 G + + VR TR +++ +P NPTG+ + ++ A+ VR + DE Sbjct: 147 GPEHRFQLSAQMVREHWGSATRGVLLASPSNPTGTSILPDELRAIVGEVRQRGGFTIVDE 206 Query: 204 VYEHMVFDGQPHESLARHPELAERSFINSSFGKTYHVTGWKVGYVAAPAALTAEFRKVHQ 263 +Y+ + ++G P +L+ L + + +SF K +++TGW++G++ P AL + K+ Q Sbjct: 207 IYQGLSYEGAPFSALS----LGDDVVVINSFSKYFNMTGWRLGWLVLPPALVPQVEKLAQ 262 Query: 264 FNVFTVNTPVQYGLAEYM--KNPAPYLDLPAFYQRKRDLFRTGLANTRFE--LLPSQGTY 319 + ++ Q+ + A Y + A ++R+RD L + F ++P G + Sbjct: 263 NLLICASSIAQHAAVACFTPQTLALYEERKAEFKRRRDYIVPALESLGFTVPVMPD-GAF 321 Query: 320 FQCVKYDAISDQSEGDFCKWLTSEIGVAAIPVSAFYNTPKESGIVRFCFAKKEETLQLAL 379 + A++D ++ + +E GV +P F +R +A E LQ A+ Sbjct: 322 YVYADCSALADDAD-QLSLDMLNEAGVVLVPGLDF-GPFTARRYIRLSYATSMENLQEAV 379 Query: 380 DRL 382 RL Sbjct: 380 ARL 382 >gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 109 bits (273), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 92/381 (24%), Positives = 165/381 (43%), Gaps = 14/381 (3%) Query: 7 VTKLPKVGTTIFTVMSALASEKGA--VNLGQGFPDFHCDPALVDAVTR-AMQDGLNQYPP 63 + +LP I + A +G +++ G PD P +V+ + A + + Y Sbjct: 44 IERLPPYVFNITAELKMAARRRGEDIIDMSMGNPDGATPPHIVEKLVEVAQRPDTHGYSS 103 Query: 64 MAGVLPLREAIADKVERLYGHRYDPVSEITVTAGATQGILTSVLCAVHPGDEVIVIEPVY 123 G+ LR AIA Y +DP SE VT G+ +G+ +L + GD V+V P Y Sbjct: 104 SKGIPRLRRAIAHWYRSRYEVDFDPDSEAIVTIGSKEGLAHLMLATLDKGDTVLVPNPSY 163 Query: 124 DCYVPAIELAGGVPVFVQMEVGAAGYSIPWDKVRAAVT---PKTRMIMVNTPHNPTGSVM 180 ++ +AG V+M G + D++ AV PK +M+++ P NPT + Sbjct: 164 PIHIYGAVIAGANIRSVRMSPGVDFF----DELERAVRESYPKPKMMILGFPSNPTAQCV 219 Query: 181 RASDVAALADIVRGTDILILSDEVYEHMVFDGQPHESLARHPELAERSFINSSFGKTYHV 240 + + R IL++ D Y + FDG S+ + P E + + K+Y++ Sbjct: 220 ELEFFERVVKLAREHQILVVHDLAYADITFDGWKAPSIMQVPGAREVAVEFFTLSKSYNM 279 Query: 241 TGWKVGYVAAPAALTAEFRKVHQFNVFTVNTPVQY-GLAEYMKNPAPYLDLPAFYQRKRD 299 GW++G++ A L A ++ ++ + TPVQ +A + + ++ A Y+R+R+ Sbjct: 280 AGWRIGFMVGNARLVAALARIKSYHDYGSFTPVQVAAIAALEGDQSCVEEIRANYERRRN 339 Query: 300 LFRTGLANTRFEL-LPSQGTYFQCVKYDAISDQSEGDFCKWLTSEIGVAAIPVSAFYNTP 358 + GL + + +P Y + +F + L + V P F Sbjct: 340 VLVKGLHEAGWMVDVPKASMYIWARIPEPYRQFGSLEFARILLEQAKVCVSPGIGFGEYG 399 Query: 359 KESGIVRFCFAKKEETLQLAL 379 E VRF + E ++ A+ Sbjct: 400 DE--YVRFALIENESRIRQAI 418 >gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 420 Score = 87.8 bits (216), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 98/375 (26%), Positives = 155/375 (41%), Gaps = 44/375 (11%) Query: 31 VNLGQGFPDFHCDPALVDAVTRAMQDGLNQYPPMAGVLPLREAIADKVERLYG-HRYDPV 89 ++LG G P H PA + GL YP G LR AIAD +++ Y R DP Sbjct: 49 ISLGIGEPK-HPTPAFIQQALIDNLAGLASYPATLGSDALRGAIADWLQKRYDIPRPDPA 107 Query: 90 SEITVTAGATQGILT---SVLCAVHPGDEVIVIEPVYDCYVPAIELAGGVPVFVQMEVGA 146 +EI G+ + + + +V+ PG V+ P Y Y + LAG P FV + Sbjct: 108 TEILPVNGSREALFSLTQTVVDPTRPGALVMCPNPFYQIYEGSAYLAGAQPYFVNSD-PK 166 Query: 147 AGYSIPWDKVRAAVTPKTRMIMVNTPHNPTGSVMRASDVAALADIVRGTDILILSDEVYE 206 ++ + +V V + +++ V +P NPTG+V+ D L + +I SDE Y Sbjct: 167 RNFAPDFSQVPQDVWERVQLLFVCSPGNPTGAVLTLEDWKELFALSDRYGFVIASDECYS 226 Query: 207 HMVFDGQPHESLARHPELAERSFIN----SSFGKTYHVTGWKVGYVAAPAALTAEF---R 259 + F+ P + L R + SS K +V G + G+VA AA+ +F R Sbjct: 227 EIYFNEDPPLGGMQAARLLGRDYRRLISFSSLSKRSNVPGMRSGFVAGDAAILKKFLLYR 286 Query: 260 KVHQFNVFTV-----------NTPVQYGLAEYMKNPAPYLDLPAFYQRKRDLFRTGLANT 308 H + V T VQ +A+Y+ F Q T L Sbjct: 287 TYHGAAMSPVIQAASVLAWQDETHVQGNIAKYVTK---------FSQ------VTPLLQE 331 Query: 309 RFELLPSQGTYFQCVKYDAISDQSEGDFCKWLTSEIGVAAIPVSAF----YNTPKESGIV 364 E+ ++ K D + + S+ +F + L +E V +P S + +G + Sbjct: 332 VLEVALPDAAFYLWAKVDKLVNISDTEFAQRLYAEYNVTVLPGSYLAREAHGVNPGAGRI 391 Query: 365 RFCF-AKKEETLQLA 378 R A+ EE L+ A Sbjct: 392 RMALVAEVEECLEAA 406 >gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 409 Score = 86.7 bits (213), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 52/195 (26%), Positives = 97/195 (49%), Gaps = 9/195 (4%) Query: 89 VSEITVTAGATQGILTSVLCAVHPGDEVIVIEPVYDCYVPAIELAGGVPVFVQMEVGAAG 148 + +I + GA++ I+ SV ++ GDEV+V P Y + A+ L+GG PV + G Sbjct: 95 IDDIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSGGTPVHYVCD-EQQG 153 Query: 149 YSIPWDKVRAAVTPKTRMIMVNTPHNPTGSVMRASDVAALADIVRGTDILILSDEVYEHM 208 + ++ +TP T+ I+V P+NPTG++ + + ++ R ++IL+DE+Y+ + Sbjct: 154 WQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQHQLIILADEIYDKV 213 Query: 209 VFDGQPHESLARHPELAERSFIN-SSFGKTYHVTGWKVGYVAAPAALTAEFRKVHQFNVF 267 ++DG H SLA + + FI + K Y G++ G++ + N+ Sbjct: 214 LYDGNTHTSLASLAD--DVLFITFNGLSKNYRSCGYRAGWMVVSGEKKHARDYIEGLNML 271 Query: 268 T-----VNTPVQYGL 277 N P Q+ + Sbjct: 272 ASMRLCANAPGQFAI 286 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,246,015 Number of extensions: 54554 Number of successful extensions: 170 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 166 Number of HSP's successfully gapped: 6 Length of query: 385 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 298 Effective length of database: 1,207,925 Effective search space: 359961650 Effective search space used: 359961650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)