BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0166.p (261 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312308|ref|YP_003776400.1| transmembrane protein [Herbaspi... 532 e-152 >gi|300312308|ref|YP_003776400.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 266 Score = 532 bits (1370), Expect = e-152, Method: Compositional matrix adjust. Identities = 254/261 (97%), Positives = 259/261 (99%) Query: 1 LKFFIIGFALSLTACGLLPEQKDETVGWSASKLYSEAKDELNAGGYDKAIKYFEKLESRY 60 LKFFIIGFALSLTACGLLPEQKDET GWSA+KLYSEAKDELNAGGYDKAIKYFEKLESRY Sbjct: 6 LKFFIIGFALSLTACGLLPEQKDETTGWSAAKLYSEAKDELNAGGYDKAIKYFEKLESRY 65 Query: 61 PFGTYAQQAQMDIAYAYYRQNEQAQGLAAVDRFIKLHPNHPNVDYMYYLRGLINFNDRTS 120 PFGTYAQQAQMDIAYAYYRQNEQAQGLAAVDRFIKLHPNHPNVDYMYYLRGLINFNDRTS Sbjct: 66 PFGTYAQQAQMDIAYAYYRQNEQAQGLAAVDRFIKLHPNHPNVDYMYYLRGLINFNDRTS 125 Query: 121 IFDTFTDQDNTERDPKAMRDAFDSFKLLAERFPDSKYTPDAIARMKYLVNAMSQYDVHVA 180 IFDTFTDQDNTERDPKAMRDAFDSFKLLAERFPDSKYTPDAIARMKYLVNAMSQYDVHVA Sbjct: 126 IFDTFTDQDNTERDPKAMRDAFDSFKLLAERFPDSKYTPDAIARMKYLVNAMSQYDVHVA 185 Query: 181 NYYFRRGAYVSAANRAQAAIKQYPDSPANEEALFILMRSYEALGQTKLKEDTERIIQATY 240 +YYFRRGAYVSAANRAQ+AIKQYPDSPANEEALFILMRSYEALGQTKLKEDTERIIQATY Sbjct: 186 SYYFRRGAYVSAANRAQSAIKQYPDSPANEEALFILMRSYEALGQTKLKEDTERIIQATY 245 Query: 241 PNSPWYNGGPKQKAKPWWKVW 261 PNSPWYNGGPK K+KPWWK+W Sbjct: 246 PNSPWYNGGPKPKSKPWWKLW 266 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 803,587 Number of extensions: 30958 Number of successful extensions: 89 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 89 Number of HSP's successfully gapped: 1 Length of query: 261 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 178 Effective length of database: 1,226,865 Effective search space: 218381970 Effective search space used: 218381970 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)