BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0159.p (616 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312303|ref|YP_003776395.1| poly(3-hydroxyalkanoate) synthe... 1087 0.0 gi|300311720|ref|YP_003775812.1| poly-beta-hydroxyalkanoate synt... 327 1e-90 gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthet... 72 2e-13 >gi|300312303|ref|YP_003776395.1| poly(3-hydroxyalkanoate) synthetase [Herbaspirillum seropedicae SmR1] Length = 588 Score = 1087 bits (2811), Expect = 0.0, Method: Compositional matrix adjust. Identities = 534/595 (89%), Positives = 555/595 (93%), Gaps = 9/595 (1%) Query: 20 MSDPKFWTSLQSQFQSQFEQFKSHLPPMPAMAGMPQMPGFPGGAMPPGAGQIDPTVLLKQ 79 MSDPKFWT+LQSQFQSQF FK PP A PGF PP Q+DPT LKQ Sbjct: 1 MSDPKFWTALQSQFQSQFAPFK---PPAQAF------PGFAAMGAPPAGSQVDPTAFLKQ 51 Query: 80 LGEHGATLDPADLVRLQQDYTREFGELWQSVLAAKPPAITDRRLSAPAWQEHPYFAFQAA 139 L ++GAT+DPA+LVRLQQDYT++FG LWQS+LAAK PAITDRRLSAPAWQEHPYFAFQAA Sbjct: 52 LADNGATIDPAELVRLQQDYTQQFGALWQSMLAAKTPAITDRRLSAPAWQEHPYFAFQAA 111 Query: 140 AYQLNSRFLLALADAVKGTEKVRHKLRFAVQQMIDAMSPANFLATNPVAQQKILETRGES 199 AYQLNSRFLLALADAV+G+EKVRHKLRFAVQQMIDAMSPANFLATNPVAQQKILETRGES Sbjct: 112 AYQLNSRFLLALADAVQGSEKVRHKLRFAVQQMIDAMSPANFLATNPVAQQKILETRGES 171 Query: 200 LTQGIAQLLADLQKGHISQTDETAFEVGRDVGASAGSVIFENELFQLIQYAPLTKTVHAV 259 LT+GIAQLLADLQKGH+SQTDETAFEVGRDVGAS GSVIFENELFQLIQY PLTKTVH V Sbjct: 172 LTKGIAQLLADLQKGHVSQTDETAFEVGRDVGASEGSVIFENELFQLIQYKPLTKTVHEV 231 Query: 260 PLLIVPPCINKFYILDLQPQNSLVRYTVEQGHTVFLISWRNADAAVETATWENYVDDAVV 319 PLLIVPPCINKFYILDLQP NSLVRYTVEQGHTVFLISWRNADAAVETATW+ YVDDAVV Sbjct: 232 PLLIVPPCINKFYILDLQPHNSLVRYTVEQGHTVFLISWRNADAAVETATWDQYVDDAVV 291 Query: 320 RAIHIVQEISKQPQINALGFCVGGTILSCALALLAARGEDPVASLTLLTTFLDFSDTGAI 379 R+IHIVQEISKQPQINALGFCVGGTILSCALALLAARGEDPVASLTLLTTFLDFSDTGAI Sbjct: 292 RSIHIVQEISKQPQINALGFCVGGTILSCALALLAARGEDPVASLTLLTTFLDFSDTGAI 351 Query: 380 DVYIDDMQLALREQMIGKRGLMAGRDFASAFSSLRPNDLLWNYVESNYLKGEKPSAFDLL 439 DVYID+MQLALREQMIG+RGLMAGRDFASAFSSLRPNDLLWNYVESNYLKGEKPSAFDLL Sbjct: 352 DVYIDEMQLALREQMIGQRGLMAGRDFASAFSSLRPNDLLWNYVESNYLKGEKPSAFDLL 411 Query: 440 YWNADSTNLPGPMFCYYLRHMYLENALKEPGRLTVGGEKIDLRKISAPAFIYASRDDHIV 499 YWNADSTNLPGPMFCYYLRHMYLENALKEPGRLTVGGEKIDLRKI+APAFIYASRDDHIV Sbjct: 412 YWNADSTNLPGPMFCYYLRHMYLENALKEPGRLTVGGEKIDLRKINAPAFIYASRDDHIV 471 Query: 500 PWKSAYASLDLINAGKRSNNRFVLGASGHIAGVINPASKNKRNYWVNDKAKAGSDADAWM 559 PWKSAYASL LIN KRSNNRFVLGASGHIAGVINPASKNKRNYW+NDKAKAGS ADAW+ Sbjct: 472 PWKSAYASLGLINTAKRSNNRFVLGASGHIAGVINPASKNKRNYWINDKAKAGSGADAWL 531 Query: 560 AGATEHPGSWWGEWAAFLAKQGGRKVAAPKAPGSTRYKAIEPAPGRYVKAKADQL 614 AGATEHPGSWWG+WAAFLAK GGRKVAAPKA GSTRYK IEPAPGRYVK KADQL Sbjct: 532 AGATEHPGSWWGQWAAFLAKHGGRKVAAPKAAGSTRYKHIEPAPGRYVKVKADQL 586 >gi|300311720|ref|YP_003775812.1| poly-beta-hydroxyalkanoate synthase [Herbaspirillum seropedicae SmR1] Length = 590 Score = 327 bits (839), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 185/495 (37%), Positives = 274/495 (55%), Gaps = 12/495 (2%) Query: 118 ITDRRLSAPAWQEHPYFAFQAAAYQLNSRFLLALADAVKGTEKVRHKL-RFAVQQMIDAM 176 + DRR S P WQ P F ++ L ++ A V G EK L FA +Q +D Sbjct: 100 VADRRFSNPDWQRWP-FNLWHQSFLLAEQWWAAATRDVWGVEKHHADLVAFAARQCLDVF 158 Query: 177 SPANFLATNPVAQQKILETRGESLTQGIAQLLADLQKGHISQTDETA--FEVGRDVGASA 234 SP+N L TNP + +E RG +L G+ + D+ + + TA F GRDV S Sbjct: 159 SPSNQLLTNPEVLRITMEQRGANLLMGMQNCMNDMIGLLLEKPASTARTFMPGRDVAVSP 218 Query: 235 GSVIFENELFQLIQYAPLTKTVHAVPLLIVPPCINKFYILDLQPQNSLVRYTVEQGHTVF 294 G V+ N + +LI+YAP T+TV PLL++P I K+YILDL P NSL+RY VE+GHTVF Sbjct: 219 GKVVLRNRIMELIRYAPATETVCPEPLLLIPAWIMKYYILDLSPANSLIRYLVEKGHTVF 278 Query: 295 LISWRNADAAVETATWENYVDDAVVRAIHIVQEISKQPQINALGFCVGGTILSCALALLA 354 +SW+N A + ++Y+D A+ V +++ +G+C+GGT+LS A A +A Sbjct: 279 CVSWKNPAPADRDLSMDDYLDSGFHAALDAVNSACPDRKVHGVGYCLGGTLLSIAAAAMA 338 Query: 355 ARGEDPVASLTLLTTFLDFSDTGAIDVYIDDMQLALREQMIGKRGLMAGRDFASAFSSLR 414 G+ +ASLTLL DF++ G + ++ID+ Q++L E + + G ++ A+AF LR Sbjct: 339 RDGDQRLASLTLLAAQTDFTEPGELGLFIDESQISLLEAQMQQAGTLSQSQMAAAFQMLR 398 Query: 415 PNDLLWNYVESNYLKGEKPSAFDLLYWNADSTNLPGPMFCYYLRHMYLENALKEPGRLTV 474 DLLW+ V YL G DL+ WNAD+T +P M YLR ++L++ L E GR V Sbjct: 399 SYDLLWSRVIRQYLMGVPAHNSDLMEWNADATRMPALMHSQYLRRLFLDDDLSE-GRYEV 457 Query: 475 GGEKIDLRKISAPAFIYASRDDHIVPWKSAYASLDLINAGKRSNNRFVLGASGHIAGVIN 534 G+ + L ++ P F+ + DH+ PW+S Y L A FVL + GH AG+++ Sbjct: 458 DGKPVLLADVNLPVFLVGTVRDHVAPWRSVYKLHSLCPA----EITFVLASGGHNAGIVS 513 Query: 535 PASKNKRNYWVNDKAKAGS--DADAWMAGATEHPGSWWGEWAAFLAKQGGRKVAAPKAPG 592 P + + ++ +A S AD WMAG + GSWW WA +LA++ G V A G Sbjct: 514 PPGHPRSQFQISTRAAGASYRSADEWMAGTSVTTGSWWPAWADWLAQRSGTPVPA-AGMG 572 Query: 593 STRYKAIEPAPGRYV 607 + ++ APG Y+ Sbjct: 573 YDKAHSLADAPGNYI 587 >gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthetase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 71.6 bits (174), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 74/285 (25%), Positives = 127/285 (44%), Gaps = 30/285 (10%) Query: 259 VPLLIVPPCINKFYIL-DLQPQNSLVRYTVEQG-HTVFLISWRNADAAVETATWENYVDD 316 VP LIV P ++ D SLV + G V LI W +A A ++ +NY+ D Sbjct: 86 VPTLIVAPYAGHTSVIADYDQGQSLVETLLGNGVDHVLLIDWHSASADMKDLEIDNYLAD 145 Query: 317 AVVRAIHIVQEISKQPQINALGFCVGGTILSCALALLAARGEDPVASLTLLTTFLDF-SD 375 V ++ E+ ++ +G C GG I A++AAR + VA L L + +D + Sbjct: 146 LCV----VIDELGG--RVRLVGLCQGGWI----SAMIAARFPEKVARLVLAGSPIDAGAG 195 Query: 376 TGAIDVYIDDMQLALREQMI-GKRGLMAGRDFASAFSSLRPN--------DLLWNYVESN 426 G + + + L E ++ GLM G + ++ P+ +LL + + Sbjct: 196 EGPLKQMVQRLPLRFYEDLVRSGGGLMRGSFMLQGWKNMHPDEHYFNEHLELLQHIDDPA 255 Query: 427 YLKGEKPSAFDLLYWNADSTNLPGPMFCYYLRHMYLENALKEPGRLTVGGEKIDLRKISA 486 YL + A W + +LPG + + H++ +N L G+ G+++DLR+I+ Sbjct: 256 YLAKRETFA----AWYETTVDLPGRWYLQAIGHIFKDNLLAR-GKYVALGQQLDLRQITC 310 Query: 487 PAFIYA-SRDDHIVPWKSAYASLDLINAGKRSNNRFVLGASGHIA 530 P ++ A RDD P + A+ + + + V G GHI Sbjct: 311 PLYLLAGERDDITTPEQVLNAAALVGTPAAQITQKIVPG--GHIG 353 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,846,211 Number of extensions: 74528 Number of successful extensions: 197 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 191 Number of HSP's successfully gapped: 3 Length of query: 616 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 525 Effective length of database: 1,188,985 Effective search space: 624217125 Effective search space used: 624217125 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)