BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0158.p (246 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 488 e-139 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 191 6e-50 gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein... 161 4e-41 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 140 1e-34 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 136 2e-33 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 488 bits (1256), Expect = e-139, Method: Compositional matrix adjust. Identities = 238/246 (96%), Positives = 241/246 (97%) Query: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSARKDTWLATMRGHGYDIHASEG 60 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNS RKD WLATMR G+DIHASEG Sbjct: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEG 60 Query: 61 NVSDWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKGDWDAVIDTNLNSLFNVT 120 NVS+WESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSK DWDAVIDTNLNSLFNVT Sbjct: 61 NVSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVT 120 Query: 121 KQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT 180 KQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT Sbjct: 121 KQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT 180 Query: 181 VSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASAEGGYATGSDFSLN 240 VSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIAS+EGGYATGSDFSLN Sbjct: 181 VSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSLN 240 Query: 241 GGLYMG 246 GGLYMG Sbjct: 241 GGLYMG 246 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 191 bits (484), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 105/248 (42%), Positives = 154/248 (62%), Gaps = 10/248 (4%) Query: 4 RIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNS--ARKDTWLATM---RGHGYDIHAS 58 ++A VTG GIG AI T L + G V+ S A +LA + G G + Sbjct: 9 QVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAGRGAVL--- 65 Query: 59 EGNVSDWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKGDWDAVIDTNLNSLFN 118 NV+D E++ A E+V+ + G + +LVNNAGIT+D +M +WD+VI TNL ++ Sbjct: 66 --NVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGR 123 Query: 119 VTKQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTV 178 +++ V+ GM + GRIINI+SV G+ G GQ NY+ AKAG+ G + ALA+E+ ++ +TV Sbjct: 124 LSRAVLRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITV 183 Query: 179 NTVSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASAEGGYATGSDFS 238 N V+PG+I TDM RA+ + I+ IP+ RLG EEIA VA++AS + Y TG+ + Sbjct: 184 NCVAPGFIDTDMTRALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTTLN 243 Query: 239 LNGGLYMG 246 +NGG+YMG Sbjct: 244 VNGGMYMG 251 >gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 245 Score = 161 bits (408), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 93/238 (39%), Positives = 132/238 (55%), Gaps = 4/238 (1%) Query: 7 YVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSARKDTWLATMRGHGYDIHASEGNVSDWE 66 +VTG GIG AI RL ++GY +V C D A + G + + +V++ E Sbjct: 10 FVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQVRVLQFDVANRE 69 Query: 67 STKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKGDWDAVIDTNLNSLFNVTKQVIEG 126 + G +V NAGITRD F M DWD+V+ TNL+ +NV ++ Sbjct: 70 QAAQVLLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFYNVLNPLVMP 129 Query: 127 MCER-GFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSPGY 185 M R GRI+ ++SV+G G GQ NYS AKAG+ G T ALA E+A + +TVN V+PG Sbjct: 130 MVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDITVNCVAPGL 189 Query: 186 IGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASAEGGYATGSDFSLNGGL 243 I TDM +++I+ IP +R G+PEE+A+ VA++ S + Y T S+NGGL Sbjct: 190 IDTDMT---EDAPIDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVISVNGGL 244 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 140 bits (352), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 87/247 (35%), Positives = 134/247 (54%), Gaps = 8/247 (3%) Query: 4 RIAYVTGGMGGIGTAICTRLCKDGYTV-VAGCGPNSARK--DTWLATMRGHGYDIHASEG 60 R+A VTGG GIG +I +L G+TV VA ++A K D +A G+ + Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAA----GHRAAPLQM 64 Query: 61 NVSDWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKGDWDAVIDTNLNSLFNVT 120 ++ ES AAF + + G DVLVNNAG+ R F +W ++ N+ + Sbjct: 65 DLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAG 124 Query: 121 KQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT 180 + M ++ +GRI+NISS++G + G+T Y T+KA + G T +A E+A G+TVN+ Sbjct: 125 QHAARLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNS 184 Query: 181 VSPGYIGTDMVRAIRPE-VLEKIVSGIPVKRLGEPEEIASIVAWIASAEGGYATGSDFSL 239 V+PG + T M +A+ E E +P++R G +EIAS V ++A+ E Y TG + Sbjct: 185 VAPGPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIPV 244 Query: 240 NGGLYMG 246 +GG G Sbjct: 245 DGGFVAG 251 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 136 bits (342), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 79/245 (32%), Positives = 128/245 (52%), Gaps = 3/245 (1%) Query: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSARKDTWLATMRGHGYDIHASEG 60 + ++A +TG GGIG A+ + + G +V G ++ + A +R G+ IH S Sbjct: 17 LEGKLALITGSSGGIGLALARGMAQAGAAIVLN-GRDAGKLAAVAAQLRAEGHTIHESSF 75 Query: 61 NVSDWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKGDWDAVIDTNLNSLFNVT 120 + + E KAA +K+ +EIG + +LVNNAGI R + + DW +I TNLNS+F Sbjct: 76 DATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDWHDLISTNLNSVFFAA 135 Query: 121 KQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT 180 + V M RG G+IINI SV + G+ G Y+ K + FT +A + G+ VN Sbjct: 136 QAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQVNG 195 Query: 181 VSPGYIGTDMVRAI--RPEVLEKIVSGIPVKRLGEPEEIASIVAWIASAEGGYATGSDFS 238 + PGY T+M A+ + + +V P +R G+ E++ ++AS + G Sbjct: 196 LGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRFVNGHILY 255 Query: 239 LNGGL 243 ++GG+ Sbjct: 256 VDGGV 260 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.134 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 821,681 Number of extensions: 33219 Number of successful extensions: 240 Number of sequences better than 1.0e-08: 48 Number of HSP's gapped: 152 Number of HSP's successfully gapped: 48 Length of query: 246 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 163 Effective length of database: 1,226,865 Effective search space: 199978995 Effective search space used: 199978995 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)