BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0010.0156.p
         (458 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312296|ref|YP_003776388.1| 2-methylthioadenine synthetase ...   939   0.0  
gi|300310058|ref|YP_003774150.1| tRNA modifying 2-methylthioaden...   154   1e-38

>gi|300312296|ref|YP_003776388.1| 2-methylthioadenine synthetase
           [Herbaspirillum seropedicae SmR1]
          Length = 458

 Score =  939 bits (2427), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 453/458 (98%), Positives = 458/458 (100%)

Query: 1   MSNPSSPATPALPKVGFVSLGCPKALVDSEQILTQLRAEGYDTAKSYDGADLVIVNTCGF 60
           MSNPSSPATPALPKVGFVSLGCPKALVDSEQILTQLRAEGYDTAKSYDGADLVIVNTCGF
Sbjct: 1   MSNPSSPATPALPKVGFVSLGCPKALVDSEQILTQLRAEGYDTAKSYDGADLVIVNTCGF 60

Query: 61  IDAAVQESLDAIGEALNENGKVIVTGCLGAKKDADGDDIIQKIHPKVLEVTGPHAVGEVM 120
           IDAAVQESLDAIGEALNENGKVIVTGCLGAKKDA+GDDIIQKIHPKVLEVTGPHAVGEVM
Sbjct: 61  IDAAVQESLDAIGEALNENGKVIVTGCLGAKKDAEGDDIIQKIHPKVLEVTGPHAVGEVM 120

Query: 121 QAVHKHLPKPHAPFIDLVPPQGVKLTPKHFAYLKISEGCNHRCSFCIIPSMRGDLVSRPI 180
           QAVHKHLPKPHAPFIDLVPPQGVKLTPKHFAYLKISEGCNHRCSFCIIPSMRGDLVSRPI
Sbjct: 121 QAVHKHLPKPHAPFIDLVPPQGVKLTPKHFAYLKISEGCNHRCSFCIIPSMRGDLVSRPI 180

Query: 181 ADVMLEAENLFKAGVKELLVISQDTSAYGVDVKFRTGFWNGKPIKTHMTQLVGALGELAQ 240
           ADVMLEAENLFKAGVKELLVISQDTSAYGVDVKFRTGFWNGKP+KTHMTQLVGALGELAQ
Sbjct: 181 ADVMLEAENLFKAGVKELLVISQDTSAYGVDVKFRTGFWNGKPVKTHMTQLVGALGELAQ 240

Query: 241 QYGAWVRLHYVYPYPHVDQIIPLMAEGKILPYLDVPLQHAHPDVLKRMKRPANGEKNIER 300
           QYGAWVRLHYVYPYPHVDQIIP+MAEGKILPYLDVPLQHAHPDVLKRMKRPANGEKNIER
Sbjct: 241 QYGAWVRLHYVYPYPHVDQIIPMMAEGKILPYLDVPLQHAHPDVLKRMKRPANGEKNIER 300

Query: 301 IQAWRALCPDITIRSTFIAGFPGETEAEFEYLLDFLKEAEIDRLGCFAYSPVEGATANEI 360
           IQAWRA+CPD+TIRSTFIAGFPGETEAEFEYLLDFLKEAEIDRLGCFAYSPVEGATANEI
Sbjct: 301 IQAWRAMCPDLTIRSTFIAGFPGETEAEFEYLLDFLKEAEIDRLGCFAYSPVEGATANEI 360

Query: 361 ANPVPEEVREERRGRVMQLQEEISKKRLQAKVGKTLRVLIDEVDRNGGTGRTYSDAPEID 420
           ANPVPEEVREERRGRVMQLQEEISKKRLQAKVGKTLRVLIDEVDRNGGTGRTYSDAPEID
Sbjct: 361 ANPVPEEVREERRGRVMQLQEEISKKRLQAKVGKTLRVLIDEVDRNGGTGRTYSDAPEID 420

Query: 421 GVVYVKPPFEPHRKLEVGQFVDVRITAADAHDLWGAAE 458
           GVVYVKPPFEPHRKLEVGQFVDVRITAADAHDLWGAAE
Sbjct: 421 GVVYVKPPFEPHRKLEVGQFVDVRITAADAHDLWGAAE 458


>gi|300310058|ref|YP_003774150.1| tRNA modifying 2-methylthioadenine
           synthetase [Herbaspirillum seropedicae SmR1]
          Length = 446

 Score =  154 bits (390), Expect = 1e-38,   Method: Compositional matrix adjust.
 Identities = 131/461 (28%), Positives = 213/461 (46%), Gaps = 43/461 (9%)

Query: 14  KVGFVSLGCPKALVDSEQILTQLRA-EGYDTAKSYDGADLVIVNTCGFIDAAVQESLDAI 72
           KV   + GC     DS+++   L A +G       + AD++++NTC   + A ++    +
Sbjct: 4   KVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVFSDL 63

Query: 73  GE--ALNENGKVIVTGCLGAKKDADGDDIIQKIHPKVLEVTGPHAVGEVMQAVHKH---- 126
           G   AL +N   ++ G  G     +G+ II++  P V  V GP  +  + Q + +     
Sbjct: 64  GRLRALKKNNPALLIGVGGCVASQEGEAIIKR-APFVDMVFGPQTLHRLPQMISERRYSG 122

Query: 127 -----LPKPHAPFIDLVPPQGVKLTPKHFAYLKISEGCNHRCSFCIIPSMRGDLVSRPIA 181
                +  P     D +PP  V+      AY+ I EGC+  CS+C++P  RG+ VSR   
Sbjct: 123 RPQVDISFPEIEKFDHLPPARVEGAT---AYVSIMEGCSKYCSYCVVPYTRGEEVSRRFE 179

Query: 182 DVMLEAENLFKAGVKELLVISQDTSAYGVDVKFRTGFWNGKPIKTHMTQLVGALGELAQQ 241
           DV+ E   L   GVKE++++ Q+ +AY      R    +G+        L+  + EL   
Sbjct: 180 DVLTEVAGLADQGVKEIMLLGQNVNAY------RGVMEDGEI--ADFALLIEYIAELP-- 229

Query: 242 YGAWVRLHYVYPYPH--VDQIIPLMAE-GKILPYLDVPLQHAHPDVLKRMKRPANGEKNI 298
                R+ +V  +P     ++I   A+  K+  +L +P QH     L  MKR     +  
Sbjct: 230 --GIERIRFVTSHPKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYK 287

Query: 299 ERIQAWRALCPDITIRSTFIAGFPGETEAEFEYLLDFLKEAEIDRLGCFAYSPVEGATAN 358
             I+  R + PDIT++S FI GFPGETE +FE L+  + +   D    F +S   G  A 
Sbjct: 288 SVIRRMRKVRPDITVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAA 347

Query: 359 EIANPVPEEVREERRGRVMQLQEEISKKRLQAKVGKTLRVLIDEVDRNGG---TGRTYSD 415
            + +  P+EV+  R  R+     + + +   A+VG   RVL++ V +       GRT S+
Sbjct: 348 NLPDDTPQEVKLARLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESN 407

Query: 416 -APEIDGVVYVKPPFEPHRKLEVGQFVDVRITAADAHDLWG 455
                DG         P+ +  +GQ ++V IT A    L G
Sbjct: 408 RVVNFDG--------GPNGQRLIGQIIEVSITEAFPFSLRG 440


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.138    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,472,473
Number of extensions: 62698
Number of successful extensions: 170
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 168
Number of HSP's successfully gapped: 2
Length of query: 458
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 370
Effective length of database: 1,203,190
Effective search space: 445180300
Effective search space used: 445180300
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 132 (55.5 bits)