BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0153.p (398 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herba... 744 0.0 gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylas... 135 9e-33 gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylas... 98 1e-21 >gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herbaspirillum seropedicae SmR1] Length = 392 Score = 744 bits (1922), Expect = 0.0, Method: Compositional matrix adjust. Identities = 361/398 (90%), Positives = 375/398 (94%), Gaps = 6/398 (1%) Query: 1 VSDSNATSPASSLPLDPQTALVHSSYRAPDGFEAFPVGIHHASTVLFPNVAAMRSRDWRE 60 +SD TS L PQT+LVHSSYRAPDGFEAFPVGIHHASTVLFP+VAAMRSRDWRE Sbjct: 1 MSDPKQTS------LTPQTSLVHSSYRAPDGFEAFPVGIHHASTVLFPDVAAMRSRDWRE 54 Query: 61 KLGYTYGLHGTPTSFVLEARLAEIEGGTYCRVTPSGLAAIAMVNFAFLKTGDDVLLPDNV 120 KLGYTYGLHGTPTSFVLEARLAEIEGG YCRVTPSGLAAIAMVNFAFLK+GDDVLLPDNV Sbjct: 55 KLGYTYGLHGTPTSFVLEARLAEIEGGKYCRVTPSGLAAIAMVNFAFLKSGDDVLLPDNV 114 Query: 121 YGPSKDLGQWLERDFGITARFYDPMVGAGLAELLQPNTRLVWTEAPGSVSMEVPDIPALC 180 YGPSKDLGQWLERDF ITARFYDPM+G GLAEL++P TRLVWTEAPGSVSMEVPDIPALC Sbjct: 115 YGPSKDLGQWLERDFQITARFYDPMIGEGLAELIRPTTRLVWTEAPGSVSMEVPDIPALC 174 Query: 181 RAAHEKGVLVAIDNTWAAGIAFKAFEHGVDIVMQAVTKYQSGGSDVLMGAVIVRDQALNE 240 RAAH KGVLVAIDNTW+AGIAFKAFEHGVDIVMQAVTKYQSGGSDVLMGAVIVRDQALNE Sbjct: 175 RAAHAKGVLVAIDNTWSAGIAFKAFEHGVDIVMQAVTKYQSGGSDVLMGAVIVRDQALNE 234 Query: 241 KLELAHMRLGFGVGMDDVYMVLRSLSSMRLRFEAHDAAARKVAAWLKQRPEIVAVLHPAF 300 KLELAHMRLGFGVGMDDVYMVLRSLSSMRLRFEAHDAAARKVAAWLKQRPEIVAVLHPAF Sbjct: 235 KLELAHMRLGFGVGMDDVYMVLRSLSSMRLRFEAHDAAARKVAAWLKQRPEIVAVLHPAF 294 Query: 301 EDCPGHAHWKRDFTGAGGLFSVIFDSRYREEQTDRFVDSLQLFKIGYSWGGAHSLCVPYR 360 EDCPGHAHWKRDF+GAGGLFSVIFD RY E QTDRFVDSL+LFKIGYSWGGAHSLCVPYR Sbjct: 295 EDCPGHAHWKRDFSGAGGLFSVIFDPRYVEAQTDRFVDSLRLFKIGYSWGGAHSLCVPYR 354 Query: 361 MRGMRHQWQREGVLVRFNIGLEAPADLIADIEQALSKL 398 M+GMR W REG LVRFNIGLE+P DLIADIEQAL++L Sbjct: 355 MKGMRRAWMREGQLVRFNIGLESPEDLIADIEQALARL 392 >gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 429 Score = 135 bits (339), Expect = 9e-33, Method: Compositional matrix adjust. Identities = 122/429 (28%), Positives = 183/429 (42%), Gaps = 47/429 (10%) Query: 13 LPLDPQ----TALVHSSYRAPDGFEAFPVGIHHASTVLFPNVAAMRSRDWREKLGYTYGL 68 +P +PQ T VH Y A V I+ F + + G Y Sbjct: 1 MPDNPQWKFETISVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSR 60 Query: 69 HGTPTSFVLEARLAEIEGGTYCRVTPSGLAAIAMVNFAFLKTGDDVLLPDNVYGPSKDLG 128 PT VLE RLA +EGG SG AA+ + GD+++ +YG + +L Sbjct: 61 IMNPTQDVLEKRLAALEGGIGALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLF 120 Query: 129 QWLERDFGITARFYDPMVGAGLAELLQPNTRLVWTEAPGSVSMEVPDIPALCRAAHEKGV 188 +G+ RF DP A L+ T+ ++ E+ G+ + DI A+ AH GV Sbjct: 121 AHTLPQYGVQTRFADPRQPASFEPLIDDRTKAIYIESIGNPLGNITDIAAVADIAHRHGV 180 Query: 189 LVAIDNTWAAGIAFKAFEHGVDIVMQAVTKYQSGGSDVLMGAV----------------- 231 + +DNT A + FEHG DIV+ ++TKY G L GA+ Sbjct: 181 PLIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKHKQRFKR 240 Query: 232 ----------IVRDQALNEKLELAHMR------LGFGVGMDDVYMVLRSLSSMRLRFEAH 275 +V +AL E + R +G + + + +L+ + ++ LR + Sbjct: 241 LNEPDVSYHGVVYTEALGEAAYIGRARVVPLRNMGAALSPFNAFQILQGIETLGLRLDRI 300 Query: 276 DAAARKVAAWLKQRPEIVAVLHPAFEDCPGHAHWKRDFTG-AGGLFSVIFDSRYREEQTD 334 A VA +LK+ P++ V + ED P HA + F G A G+ + F E Sbjct: 301 TANTLAVAQYLKRHPKVRWVNYAGLEDHPDHALAQTYFKGRASGVLT--FGVANGREGGA 358 Query: 335 RFVDSLQLFKIGYSWGGAHSLCVPYRMRGMRH----QWQREGV---LVRFNIGLEAPADL 387 RF D+LQLF + G A SL R + ++ GV VR +IG+E DL Sbjct: 359 RFQDALQLFTRLVNIGDAKSLATHPASTTHRQLSPTELEKAGVTEDTVRLSIGIEHIDDL 418 Query: 388 IADIEQALS 396 +AD+EQAL Sbjct: 419 LADLEQALQ 427 >gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 413 Score = 98.2 bits (243), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 103/411 (25%), Positives = 169/411 (41%), Gaps = 47/411 (11%) Query: 16 DPQTALVHSSYRAPDGFEAFPVGIHHASTVLFPNVAAMRSRDWREKLGYTYGLHGTPTSF 75 D Q + H S P IH + T + + + ++ GY YG G PT Sbjct: 17 DRQKGIEHGSLHKP---------IHTSVTFGYEDARQLAEVFQGKQPGYRYGRQGNPTVA 67 Query: 76 VLEARLAEIEGGTYCRVTPSGLAAIAMVNFAFLKTGDDVLLPDNVYGPSKDLGQWLERDF 135 LE ++ ++E G +G+AAI + L+ GD V+ ++G + L W+ Sbjct: 68 ALEDKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFGNTNSL--WMTVGA 125 Query: 136 -GITARFYDPMVGAGLAELLQPNTRLVWTEAPGSVSMEVPDIPALCRAAHEKGVLVAIDN 194 G D + + NTRLV+ E + +V D+ + E+G+L +DN Sbjct: 126 QGAKVSMVDATDVKNVEAAITANTRLVFVETIANPRTQVADLKRIGELCRERGILYVVDN 185 Query: 195 TWAAGIAFKAFEHGVDIVMQAVTKYQSGGSDVLMGAVIVRDQ---------ALNEKLE-- 243 T + F+ G +V+ ++TK G + L GA+ + A N K Sbjct: 186 TMTSPYLFRPKTVGAGLVVNSLTKSIGGHGNALGGALTDTGEFDWTRYPHIAENYKKNPA 245 Query: 244 ----LAHMR------LGFGVGMDDVYMVLRSLSSMRLRFEAHDAAARKVAAWLKQRPEIV 293 +A +R G +G + + + ++ LR E A +A L+ + Sbjct: 246 PQWGMAQIRAKALRDFGGSLGPEAAHHIAVGAETIALRQERECKNALALAQMLQADERVA 305 Query: 294 AVLHPAFEDCPGHAHWKRDFTGAGGLFSVIFDSRYREEQTDRFVDSLQLFKIGYSWGGAH 353 AV +P E P HA K F G L S F+ + + D +++ L+L + G Sbjct: 306 AVYYPGLESHPQHALSKALFRSFGSLMS--FELKDGIDCFD-YLNRLRLAIPTSNLGDTR 362 Query: 354 SLCVP------YRMRGMRHQWQREGV---LVRFNIGLEAPADLIADIEQAL 395 +L +P Y M R G+ L+R ++GLE DL+AD QAL Sbjct: 363 TLVIPVAHTIFYEMGAERRASM--GIAESLIRVSVGLEDTDDLVADFRQAL 411 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,398,038 Number of extensions: 62335 Number of successful extensions: 151 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 147 Number of HSP's successfully gapped: 3 Length of query: 398 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 311 Effective length of database: 1,207,925 Effective search space: 375664675 Effective search space used: 375664675 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)