BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0130.p (433 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312274|ref|YP_003776366.1| citrate synthase [Herbaspirillu... 889 0.0 gi|300312419|ref|YP_003776511.1| methylcitrate synthase [Herbasp... 178 6e-46 gi|300310601|ref|YP_003774693.1| citrate synthase [Herbaspirillu... 75 1e-14 >gi|300312274|ref|YP_003776366.1| citrate synthase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 889 bits (2296), Expect = 0.0, Method: Compositional matrix adjust. Identities = 423/433 (97%), Positives = 427/433 (98%) Query: 1 MSNTDIKATLSFSDGSPSVDFPIYKGSIGPDVIDIRKLYGATGKFTYDPGFMSTASCNSS 60 MSNTDIKATLSFSDGSPSVDFP+YKGSIGPDVIDIRKLYGATGKFTYDPGFMSTASCNSS Sbjct: 24 MSNTDIKATLSFSDGSPSVDFPVYKGSIGPDVIDIRKLYGATGKFTYDPGFMSTASCNSS 83 Query: 61 ITYIDGDKGELLYRGYPIEQLAVNCDFLESCYLLLHGELPNATQKEEFVKTVTQHTMVHE 120 ITYIDGDKGELLYRGYPIEQLAVNCDFLESCYLLLHGELPNA QK+ FV TVTQHTMVHE Sbjct: 84 ITYIDGDKGELLYRGYPIEQLAVNCDFLESCYLLLHGELPNAEQKKVFVNTVTQHTMVHE 143 Query: 121 QMNFFFRGFRRDAHPMAVLTGSVGALSAFYHDSLDISNPNHREVSAIRMIAKLPTLVAMA 180 QMNFFFRGFRRDAHPMAVLTGSVGALSAFYHDSLDISN NHREVSAIRMIAKLPTLVAMA Sbjct: 144 QMNFFFRGFRRDAHPMAVLTGSVGALSAFYHDSLDISNANHREVSAIRMIAKLPTLVAMA 203 Query: 181 YKYSIGQPFVYPRNDLSYTANFMRMMFANPCEEYQVNEVLVRALDRILILHADHEQNAST 240 YKYSIGQPFVYPRNDLSYTANFMRMMFANPCEEYQVNEVLVRALDRILILHADHEQNAST Sbjct: 204 YKYSIGQPFVYPRNDLSYTANFMRMMFANPCEEYQVNEVLVRALDRILILHADHEQNAST 263 Query: 241 STVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLEQIGSVENIPEFIKQVKDKN 300 STVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLEQIGSV+ IPEFIKQVKDKN Sbjct: 264 STVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLEQIGSVDKIPEFIKQVKDKN 323 Query: 301 SGVKLMGFGHRVYKNYDPRAKLMRETCYEVLNELGLHDDPLFKLAMELEKIALEDDYFVS 360 SGVKLMGFGHRVYKNYDPRAKLMRETCYEVLNELGLHDDPLFKLAMELEKIALED+YFVS Sbjct: 324 SGVKLMGFGHRVYKNYDPRAKLMRETCYEVLNELGLHDDPLFKLAMELEKIALEDEYFVS 383 Query: 361 RKLYPNVDFYSGIVQRALGIPTSMFTCIFAMARTIGWIAQWNEMISDPEQKIGRPRQLFV 420 RKLYPNVDFYSGIVQRALGIPTSMFTCIFAMARTIGWIAQWNEMISDPEQKIGRPRQLFV Sbjct: 384 RKLYPNVDFYSGIVQRALGIPTSMFTCIFAMARTIGWIAQWNEMISDPEQKIGRPRQLFV 443 Query: 421 GSPRRDVSPIDKR 433 GSPRRDV PIDKR Sbjct: 444 GSPRRDVPPIDKR 456 >gi|300312419|ref|YP_003776511.1| methylcitrate synthase [Herbaspirillum seropedicae SmR1] Length = 384 Score = 178 bits (452), Expect = 6e-46, Method: Compositional matrix adjust. Identities = 118/378 (31%), Positives = 195/378 (51%), Gaps = 20/378 (5%) Query: 58 NSSITYIDGDKGELLYRGYPIEQLAVNCDFLESCYLLLHGELPNATQKEEFVKTVTQHTM 117 N+++ + +L YRGY I +A +C+F E +LL+HG+LP A + + + Sbjct: 24 NTALCTVGKTGNDLHYRGYDILDVADSCEFEEIAHLLVHGKLPTAAELRAYKAKLKSLRG 83 Query: 118 VHEQMNFFFRGFRRDAHPMAVLTGSVGALSAFYHDSLDISNPNHREVSAIRMIAKLPTLV 177 + + +HPM V+ V AL + D + P R++ A R++A L +++ Sbjct: 84 LPANVKAALEWLPAASHPMDVMRTGVSALGCVLPEKDDHNTPGARDI-ADRLMASLGSML 142 Query: 178 AMAYKYSI-GQPFVYPRNDLSYTANFMRMMFA-NPCEEYQVNEVLVRALDRILILHADHE 235 Y YS G +D S A+F+ ++ P E ++ +A+ LIL+A+HE Sbjct: 143 LYWYHYSHNGNRIEVETDDDSIGAHFLHLLHGEKPSEAWE------KAMHTSLILYAEHE 196 Query: 236 QNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLEQIGSVENIPEFIKQ 295 NAST T R+ +G++ ++ I I L GP HGGANE A + ++ + + I++ Sbjct: 197 FNASTFTGRVIAGTGSDMYSAITGAIGALRGPKHGGANEVAFEIQKRYDNPDEAEADIRR 256 Query: 296 VKDKNSGVKLMGFGHRVYKNYDPRAKLMRETCYEVLNELGLHDDPLFKLAMELEKIALED 355 + +N V ++GFGH VY DPR K+++E ++ E G +F +A LE + + Sbjct: 257 -RVENKEV-VIGFGHPVYTISDPRNKVIKEVARKLSKEAG--STKMFDIAERLETVMWD- 311 Query: 356 DYFVSRKLYPNVDFYSGIVQRALGIPTSMFTCIFAMARTIGWIAQWNEMISDPEQKIGRP 415 +K++PN+D++S + +G+PT+MFT +F +ART GW A E D KI RP Sbjct: 312 ----IKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTSGWAAHIIEQRID--NKIIRP 365 Query: 416 RQLFVGSPRRDVSPIDKR 433 +VG PI KR Sbjct: 366 SANYVGPEDLKFVPISKR 383 >gi|300310601|ref|YP_003774693.1| citrate synthase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 74.7 bits (182), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 58/195 (29%), Positives = 95/195 (48%), Gaps = 23/195 (11%) Query: 228 LILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLEQIGSVE 287 L+L ADHE NAS+ T R S+ A+ A + AG+A L G HG + +++G+ Sbjct: 217 LVLCADHELNASSFTSRCVASTDASLRAVVIAGLAGLSGGRHGATTGRVEALWDELGTR- 275 Query: 288 NIPEFIKQVKDKNS-GVKLMGFGHRVYKNYDPRAKLMRETCYEVLNELGLHDDPLFKLAM 346 + ++++++ S G L GFGH +Y + DPRA + L+++ P Sbjct: 276 ---DVDRKMRERLSRGDDLPGFGHTLYPDGDPRASYL-------LSQILPRHSPW----- 320 Query: 347 ELEKIALEDDYFVSRKLYPNVDFYSGIVQRALGIPTSMFTCIFAMARTIGWIAQWNEMIS 406 A+ D P+VDF ++R L +P +FA+ R+IGWIA E Sbjct: 321 ----AAMIDAGITLTGQKPSVDFALVALRRHLRLPVGAAFGLFALGRSIGWIAHGLEQRE 376 Query: 407 DPEQKIGRPRQLFVG 421 + + RPR +++G Sbjct: 377 --QHHLIRPRAVYIG 389 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,331,403 Number of extensions: 53481 Number of successful extensions: 128 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 123 Number of HSP's successfully gapped: 3 Length of query: 433 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 345 Effective length of database: 1,203,190 Effective search space: 415100550 Effective search space used: 415100550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)