BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0094.p (393 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembr... 687 0.0 gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herb... 342 2e-95 gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux ... 136 3e-33 gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herb... 115 7e-27 gi|300312340|ref|YP_003776432.1| transporter transmembrane prote... 62 6e-11 >gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 395 Score = 687 bits (1774), Expect = 0.0, Method: Compositional matrix adjust. Identities = 365/379 (96%), Positives = 372/379 (98%) Query: 1 MPIALWALTISAFAIGTTEFVIMGLLPEVATNLGVSLSSAGLLVTGYALGVFVGAPLVTA 60 MPIALWALTISAFAIGTTEFVIMGLLPEVA NLGVSLSSAGLLVTGYALGVFVGAPL+TA Sbjct: 1 MPIALWALTISAFAIGTTEFVIMGLLPEVAANLGVSLSSAGLLVTGYALGVFVGAPLLTA 60 Query: 61 TTGRLPRKTLLIALMVVFVIGNLACAIAPGYVTLMIGRVVASFAHGAFFGVGSVVATSLV 120 TTGRLPRKTLL+ALMVVFV+GNLACAIAP Y TLMIGRVVASFAHGAFFGVGSVVATSLV Sbjct: 61 TTGRLPRKTLLVALMVVFVVGNLACAIAPDYTTLMIGRVVASFAHGAFFGVGSVVATSLV 120 Query: 121 PKEKQASAIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPR 180 PKEKQASAIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPR Sbjct: 121 PKEKQASAIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPR 180 Query: 181 SQGGEEADFGKEVRVLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDISGFPASAVSPI 240 SQGGEE DFGKEVRVLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTD+SGFPASAVSPI Sbjct: 181 SQGGEETDFGKEVRVLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDLSGFPASAVSPI 240 Query: 241 LLLFGLGLVIGNLAGGKLADRKLMATLLGSLVLLAVVLVLFGLTNENKIAAIIGVGLMGM 300 LLLFGLGLVIGNLAGGKLAD+KLMATLLGSLVLLA VLVLFGLT +NK+AAIIGVGLMGM Sbjct: 241 LLLFGLGLVIGNLAGGKLADKKLMATLLGSLVLLAAVLVLFGLTVDNKVAAIIGVGLMGM 300 Query: 301 AAFATVPPLQMRVLEKATGAPNLASSANIAAFNLGNAAGAWLGGVTIDHGPGLHAVPYVA 360 AAFATVPPLQMRVLEKATGAPNLASSANIAAFNLGNAAGAWLGGVTIDHGPGLHAVPYVA Sbjct: 301 AAFATVPPLQMRVLEKATGAPNLASSANIAAFNLGNAAGAWLGGVTIDHGPGLHAVPYVA 360 Query: 361 ALLPLAGIAVALVSWKMDA 379 ALLPLAGI VALVSWKMDA Sbjct: 361 ALLPLAGIVVALVSWKMDA 379 >gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 385 Score = 342 bits (878), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 190/376 (50%), Positives = 251/376 (66%) Query: 5 LWALTISAFAIGTTEFVIMGLLPEVATNLGVSLSSAGLLVTGYALGVFVGAPLVTATTGR 64 L+AL ++AF IGTTEFVIMGLLP+VA +LGV+ +AG+LV+GYALGV GAP++ T + Sbjct: 5 LFALAVAAFGIGTTEFVIMGLLPDVARDLGVTAPAAGMLVSGYALGVAAGAPILAILTAK 64 Query: 65 LPRKTLLIALMVVFVIGNLACAIAPGYVTLMIGRVVASFAHGAFFGVGSVVATSLVPKEK 124 PRK L+ LM +F++GN+ CA+AP YV LM+ RVV +F H AFFG+GSVVA LV +K Sbjct: 65 WPRKQALVGLMCLFILGNVLCALAPNYVFLMVARVVTAFCHAAFFGIGSVVAAELVAPQK 124 Query: 125 QASAIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPRSQGG 184 +A A+ALMFTGLTVAN+LGVP GT G + GWR+TF AVT IGV+A ++P+ Sbjct: 125 RAQAVALMFTGLTVANILGVPLGTAFGHAAGWRSTFGAVTAIGVLAAIMLWTMLPKKIPM 184 Query: 185 EEADFGKEVRVLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDISGFPASAVSPILLLF 244 + + +E R ++ +V L LL + L +F+ TYIA IL D++GF S +LLLF Sbjct: 185 QAGNIFQEFRSIIDTRVQLSLLTSTLASISMFSVITYIAYILLDVTGFTTQQESWVLLLF 244 Query: 245 GLGLVIGNLAGGKLADRKLMATLLGSLVLLAVVLVLFGLTNENKIAAIIGVGLMGMAAFA 304 G G+ +G L GGKLAD KLM + G + +AVVL LF +T+ K + + G AFA Sbjct: 245 GAGITVGGLVGGKLADWKLMPVMCGVFLCIAVVLGLFSITSHYKWPTLATMFAWGAFAFA 304 Query: 305 TVPPLQMRVLEKATGAPNLASSANIAAFNLGNAAGAWLGGVTIDHGPGLHAVPYVAALLP 364 VP Q+RVL+ A GAPNLAS+ N AFNLGNA GAW+GG+ I G L +P + A Sbjct: 305 VVPGAQIRVLDTARGAPNLASTLNQGAFNLGNATGAWVGGLAISAGLPLTDLPLIGAFFA 364 Query: 365 LAGIAVALVSWKMDAR 380 L G+A S +D R Sbjct: 365 LLGLATVAFSASLDKR 380 >gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 136 bits (342), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 122/392 (31%), Positives = 197/392 (50%), Gaps = 37/392 (9%) Query: 8 LTISAFAIGTTEFVIMGLLPEVATNLGVSLSSAGLLVTGYALGVFVGAPLVTATTGRLPR 67 L + FAIGT F+I LLP +A + G+S+ LV + L + + +P+ T + + R Sbjct: 29 LALGTFAIGTEGFMIAPLLPRMAADFGMSVPMVASLVVIFTLVLALSSPISTVLSAAMNR 88 Query: 68 KTLLIALMVVFVIGNLACAIAPGYVTLMIGRVVASFAHGAFFGVGSVVATSLVPKEKQAS 127 + +LIA M F NL A + + L++ R++ + A G + S +A ++V ++ Sbjct: 89 RRVLIAAMTAFTAANLLAACSSSFTGLLVARLLMAVAAGLYTPNASALAGAMVAPGQRGR 148 Query: 128 AIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPRSQGG--E 185 A+A++ G+T + LG+P G +G ++GWRATF AV+ +G +ALA + VPR G Sbjct: 149 ALAIVSGGMTSPSALGLPLGAVVGHAFGWRATFLAVSAMGAVALAGILLGVPRQAGAHLH 208 Query: 186 EADFGKEVRVLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDISGFPASAVSPILLLFG 245 A + + V+ +PQV L +T+L G +AA+ YIAP L+ + F S +S + L+G Sbjct: 209 VAGLRERMAVIGQPQVQRLLAVTLLWSVGAYAAYPYIAPYLSHVLSFGESRISLTVSLWG 268 Query: 246 LGLVIGNLAGGKLADRKLMATLLGSLVLLAVVLVLFGLTNENKIAA------------II 293 +G GG L DR GS ++ V L+L L+ AA ++ Sbjct: 269 AAAAVGVSVGGTLNDRY------GSARVVRVSLLLLMLSFWTLAAATALPPPLALGPVLL 322 Query: 294 GVGLMGMAAFATVPPLQMRVL-----EKATGAPNLASSANIAAFNLGNAAGAWLGGVTID 348 V + G + +A P R++ +A A +L +S F+LG+AAGA + G Sbjct: 323 AVVVWGFSVWAFFPAQMARLIGAGAPAQAPVALSLNTSTLYLGFSLGSAAGAGILGSGAI 382 Query: 349 HGPGLHAVPYVAALLPLAGIAVALVSWKMDAR 380 G GL A A ALV+W ++ R Sbjct: 383 WGIGLFAG------------ACALVAWLLERR 402 >gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 422 Score = 115 bits (288), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 105/343 (30%), Positives = 177/343 (51%) Query: 7 ALTISAFAIGTTEFVIMGLLPEVATNLGVSLSSAGLLVTGYALGVFVGAPLVTATTGRLP 66 +L + F++ T EF+ LL +A++L VS +AG VT A+ V AP + T + Sbjct: 41 SLALGVFSLVTAEFLPASLLTAIASDLHVSDGAAGQAVTATAIVGAVAAPSIPLLTRNID 100 Query: 67 RKTLLIALMVVFVIGNLACAIAPGYVTLMIGRVVASFAHGAFFGVGSVVATSLVPKEKQA 126 RK +++ L ++ ++ N+ AP L++ RV+ A G F+ + + +A LVP A Sbjct: 101 RKRVVLMLSLLLLVSNILAVTAPNLPVLLVARVLLGVALGGFWSMAAALAMRLVPDHLFA 160 Query: 127 SAIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPRSQGGEE 186 A++L+ TG++VA V P G ++G +GWR+ F A V+ ++A+A + +PR + Sbjct: 161 RAMSLILTGVSVATVCAAPIGAYMGNLWGWRSAFVAAGVVSLVAIAIQWIAIPRLPPKDH 220 Query: 187 ADFGKEVRVLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDISGFPASAVSPILLLFGL 246 +L R V + LL +L G FA FTYI P++ I+ + +S +LL +G+ Sbjct: 221 PSLKVLAELLGRKDVRVALLAVLLVVSGHFAGFTYIRPLMEQITHMSVTTISAVLLGYGV 280 Query: 247 GLVIGNLAGGKLADRKLMATLLGSLVLLAVVLVLFGLTNENKIAAIIGVGLMGMAAFATV 306 G GN AGG +A+R ++ L+A++ + + + A + + L G A A Sbjct: 281 GGFFGNFAGGFIAERSERYAIVSGGALIALLAASLWVAGSSPVVAAVAITLWGFAFGAFP 340 Query: 307 PPLQMRVLEKATGAPNLASSANIAAFNLGNAAGAWLGGVTIDH 349 Q ++ A A +AAF + A+GA GGV +DH Sbjct: 341 VGFQTWIVRAAPDHAEGAGGLLVAAFQVAIASGAIGGGVLVDH 383 >gi|300312340|ref|YP_003776432.1| transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 421 Score = 62.4 bits (150), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 69/291 (23%), Positives = 121/291 (41%), Gaps = 17/291 (5%) Query: 32 NLGVSLSSAGLLVTGYALGVFVGAPLVTATTGRLPRKTLLIALMVVFVIGNLACAIAPGY 91 ++ ++ GL+V+ YA L R RK LL+ FV+G + C +A + Sbjct: 51 SMQITPQQFGLVVSAYAFSAGASGLLTAGFADRFDRKRLLLFFYGGFVLGTVWCGLAQSF 110 Query: 92 VTLMIGRVVASFAHGAFFGVGSVVATSLVPKEKQASAIALMFTGLTVANVLGVPFGTWLG 151 +L++ R+V G + +AT L + + ++ T + VLG+P G +L Sbjct: 111 ESLLLARIVTGLFGGVIGSITLAIATDLFAPHLRGRVMGVIQTSFAASQVLGIPIGLYLS 170 Query: 152 QSYGWRATFWAVTVIGVIALAATAVLVPRSQ--------GGEEADFGKEVRVLMRPQVML 203 + W F A+ +G LA V++ R Q E + + L P+ + Sbjct: 171 NRWNWHVPFLAMAALG---LAGGLVVLVRMQPVDGHLTLKQEHSAWMHLYHTLAEPRYLT 227 Query: 204 GLLMTVLGFGGVFAAFTYIAPILTDISGFPASAVSPILLLFGLGLVIGNLAGGKLADR-- 261 T L G F + + L G + + I L+ GL + G+ AD+ Sbjct: 228 AFATTALLMTGGFMLMPFSSAYLVGNLGIDMAHLPTIYLVTGLCTIAFGPLIGRAADKLG 287 Query: 262 KLMATLLGSLVLLAVVLVL--FGLTNENKIAAIIGVGLMGMAAFATVPPLQ 310 KL L G+++ + +VL+ G I A+ V +G+ F+ + P Q Sbjct: 288 KLRVFLFGAVLSIIMVLIYTHLGRVTVPMIVAVNAVLFLGI--FSRMIPFQ 336 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.139 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,022,557 Number of extensions: 37658 Number of successful extensions: 330 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 332 Number of HSP's successfully gapped: 18 Length of query: 393 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 306 Effective length of database: 1,207,925 Effective search space: 369625050 Effective search space used: 369625050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)