BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0010.0085.p
         (354 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309800|ref|YP_003773892.1| isopenicillin N synthase [Herba...   182   4e-47

>gi|300309800|ref|YP_003773892.1| isopenicillin N synthase
           [Herbaspirillum seropedicae SmR1]
          Length = 328

 Score =  182 bits (462), Expect = 4e-47,   Method: Compositional matrix adjust.
 Identities = 116/315 (36%), Positives = 170/315 (53%), Gaps = 11/315 (3%)

Query: 11  LPVIDISGLYGDAPTRRQAVADQLGAAAREVGFFYITGHGIDPQLIAGLREASRALFAQP 70
           +PVID +  +     RR+A+A ++   +R+VGFFY+  HG+   LI G  + +R  FA P
Sbjct: 13  IPVIDFADAFSPELARREALAWEIHKISRDVGFFYLVNHGVPQALIDGQFDWARRFFALP 72

Query: 71  MPWKMRYYIGDAWGHKGYVPEGEEVYSKGRP-DHKEAFDIGFEVPDDHPLVLAGTPLIGN 129
              K    +  +    GY   G +   +G P D KE F  GF++  DHP VL G    G+
Sbjct: 73  QASKSAIDMRHSASGYGYERMGAQALDEGSPADLKEGFQFGFDIAPDHPYVLRGLLRYGH 132

Query: 130 NKWPE-LPGFKEAVSRYYEAVFALGRTLFRGFALALGQPEDAFESLVTCPPSKLRLIHYP 188
           N WP+ LPGF+    +YYEAV AL   L    AL+L  PED F  ++  P +  R++HYP
Sbjct: 133 NLWPQDLPGFETHSRQYYEAVRALSHRLLGVIALSLEMPEDFFTPVLHTPIATQRMLHYP 192

Query: 189 VDLEAV--DAPGIGAHTDYECFTLLLADQP-GLEVMNDQGAWIDAPPCSVAGEEAFVINI 245
               +   +  G GAHTD+   T+L  D   GLE+ N +G W+ APP  +AG  +FV+NI
Sbjct: 193 PQPASAQHNQIGAGAHTDWGLVTILAQDAIGGLEICNAEGDWVSAPP--IAG--SFVVNI 248

Query: 246 GDMLEVMTAGAFPATSHRVRKV-QQERYSFPLFFACDYHTRIRPLPQFASGSEDATRYGE 304
           GD+L+  +   + + +HRV  V    RYS P F   D    +  LP   S +E   RY  
Sbjct: 249 GDLLQRWSNDLYHSNAHRVCNVGNAPRYSLPFFQDGDQAAVVACLPTCCS-AERPARYAP 307

Query: 305 LSIGEHMYSQAQQTY 319
            +IG+++  + +QT+
Sbjct: 308 CTIGDYLEMKVKQTF 322


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.138    0.427 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,230,980
Number of extensions: 55784
Number of successful extensions: 140
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 134
Number of HSP's successfully gapped: 1
Length of query: 354
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 268
Effective length of database: 1,212,660
Effective search space: 324992880
Effective search space used: 324992880
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 131 (55.1 bits)