BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0084.p (270 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311472|ref|YP_003775564.1| amino acid ABC transporter/sign... 89 6e-19 gi|300311601|ref|YP_003775693.1| glutamine ABC transporter perip... 73 2e-14 gi|300313610|ref|YP_003777702.1| amino acid ABC transporter/sign... 66 4e-12 gi|300312810|ref|YP_003776902.1| glutamine ABC transporter/signa... 57 1e-09 >gi|300311472|ref|YP_003775564.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 267 Score = 88.6 bits (218), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 70/230 (30%), Positives = 114/230 (49%), Gaps = 14/230 (6%) Query: 35 GQLKVGMEITYPPF--ESWQGDKVVGFDPDIAEMLGRELKLRPSFIDTKFPSLILGLGGG 92 G LKV +E YPPF + + ++ GF+ D+A++L +L ++P F T++ ++ GLG G Sbjct: 37 GTLKVALEGNYPPFNFKDPKTGELTGFEVDVAKLLAAKLGVKPVFTTTEWSGILAGLGAG 96 Query: 93 QFDAIISGMYITPERTAQAD-AIAYGKTGAAIMVAKDGGVMPKSENDLCGLKVGLQAGTS 151 ++D II+ + IT ER D + Y + A ++V KD KS DL G K+GL GT+ Sbjct: 97 KYDVIINQVGITDERQKAFDFSDPYTLSSAQLIVRKDEKREFKSLEDLKGKKLGLGQGTN 156 Query: 152 WVNALKKLSAEYCVPQGKPAVAVSEFPTAPEASQALLSKNIQAQMEIAGAAKMLAERSKG 211 + K + P + V +P +PE L + I A + + + +S Sbjct: 157 FEQKAKAV----------PGIDVRTYPGSPEYLADLAAGRIDAALNDSLLVGYIL-KSTN 205 Query: 212 RIVISSQALVYPQTLGIYVKKGNAALYQALQKALAAGHASGEYAALIKKY 261 + + + +GI +KGN AL KALA A G + A +K+ Sbjct: 206 LPLKAGSPIGAVDKIGIPFRKGNPEFKAALNKALADIKADGSFKAASEKW 255 >gi|300311601|ref|YP_003775693.1| glutamine ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 251 Score = 72.8 bits (177), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 62/226 (27%), Positives = 100/226 (44%), Gaps = 12/226 (5%) Query: 37 LKVGMEITYPPFESWQGDKVVGFDPDIAEMLGRELKLRPSFIDTKFPSLILGLGGGQFDA 96 ++VG + T+PP E + K GFD ++ E L + + + +ID F LI GL +FD Sbjct: 32 IRVGTDATFPPMEFVKDGKRTGFDVELIEALAKTMGKKVQWIDIDFKGLIPGLVSNRFDI 91 Query: 97 IISGMYITPERTAQAD-AIAYGKTGAAIMVAKDGGVMPKSENDLCGLKVGLQAGTSWVNA 155 S +Y+T ER + + Y + G A++V +D + E+ G +V +Q GT V Sbjct: 92 AASAIYMTDERRKVVNFSDPYYRGGLAVLVRRDDNSIKVPEDLNKGKRVSVQVGTKSVGY 151 Query: 156 LKKLSAEYCVPQGKPAVAVSEFPTAPEASQALLSKNIQAQMEIAGAAKMLAERSKGRIVI 215 LK P V E L + + A + AA A R++ + + Sbjct: 152 LK---------DNFPGVERVEVEKNQAMFDLLATGRVNAVVTGRPAAVEYA-RTQPLVRV 201 Query: 216 SSQALVYPQTLGIYVKKGNAALYQALQKALAAGHASGEYAALIKKY 261 + L + G ++K + L L KAL +G Y AL K+ Sbjct: 202 LDKGLT-TELYGFALRKSDTELTAQLNKALQTLRMNGTYTALTDKW 246 >gi|300313610|ref|YP_003777702.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 271 Score = 65.9 bits (159), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 63/231 (27%), Positives = 101/231 (43%), Gaps = 21/231 (9%) Query: 39 VGMEITYPPFESWQGDK-VVGFDPDIAEMLGRELKLRPSFIDTKFPSLILGLGGGQFDAI 97 VG+E Y PF S K VVGFD DI + L +++ + F+ T F L G D + Sbjct: 51 VGVESAYAPFSSENEQKDVVGFDIDIMKALAKKIGIEVKFVPTPFEGFFNFLAQGDRDLL 110 Query: 98 ISGMYITPERTAQADAIAYGKTGAAIMVAKDGGVMPKSEN------DLCGLKVGLQAGTS 151 IS + IT ER ++A+ + VA +P ++ DL L VG Q+ TS Sbjct: 111 ISAITITDERKK---SVAFSE---PYFVATQTIALPAADTKVSKMEDLKPLTVGTQSATS 164 Query: 152 WVNALKKLSAEYCVPQGKPAVAVSEFPTAPEASQALLSKNIQAQMEIAGAAK-MLAERSK 210 ++++ GK + + F + P A + L S + A + K +A Sbjct: 165 GDELVQQV-------LGKNSAKIKRFDSTPLALKELESGGVDAVVADEPVVKNYIANNPN 217 Query: 211 GRIVISSQALVYPQTLGIYVKKGNAALYQALQKALAAGHASGEYAALIKKY 261 ++ + + GI V+K + L + K LA A G +AA+ +Y Sbjct: 218 SKLRTVTDPSFPKEDYGIAVRKDDPELLAKINKGLAEMKADGSFAAISAQY 268 >gi|300312810|ref|YP_003776902.1| glutamine ABC transporter/signal transduction system periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 249 Score = 57.4 bits (137), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 73/255 (28%), Positives = 113/255 (44%), Gaps = 28/255 (10%) Query: 14 LLTLALSALLPVAASAQTMVPGQLKVGMEITYPPFESWQGDKVVGFDPD----IAEMLGR 69 L+ +L AL V ASA+ L V + + PFE ++ VGFD D IA+ +G Sbjct: 8 LVFASLVALSVVGASARAET---LVVACDTAFVPFEFKDRNQYVGFDIDLWDAIAKDIGV 64 Query: 70 ELKLRPSFIDTKFPSLILGLGGGQFDAIISGMYITPERTAQAD-AIAYGKTGAAIMVAKD 128 KL+P F +I L D ++G+ I ER D + Y +G ++MV Sbjct: 65 TYKLQP----MDFNGIIPALQTKNVDVALAGITIKDERKKVIDFSDGYYDSGFSLMVPVG 120 Query: 129 GGVMPKSENDLCGLKVGLQAGTSWVNALKKLSAEYCVPQGKPAVAVSEFPTAPEASQALL 188 + KS DL G + +++GTS + +Y K A + +FP A L Sbjct: 121 SSI--KSVADLAGKALAVKSGTS--------AFDYAKANFK-ATELHQFPNIDNAYLELQ 169 Query: 189 SKNIQAQM-EIAGAAKMLAERSKGRI-VISSQALVYPQTLGIYVKKGNAALYQALQKALA 246 + + A M + +A KGR V+ +Q + + GI KG + L + ALA Sbjct: 170 TGRVDAAMHDTPNVLYYIATAGKGRAKVVGTQMMAH--QYGIGFPKG-SPLVPKVNAALA 226 Query: 247 AGHASGEYAALIKKY 261 A G YA + +K+ Sbjct: 227 KIKADGRYAEIYRKW 241 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 760,047 Number of extensions: 29337 Number of successful extensions: 132 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 134 Number of HSP's successfully gapped: 6 Length of query: 270 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 186 Effective length of database: 1,222,130 Effective search space: 227316180 Effective search space used: 227316180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)