BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0081.p (314 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310460|ref|YP_003774552.1| D-3-phosphoglycerate dehydrogen... 247 1e-66 gi|300311801|ref|YP_003775893.1| D-isomer specific 2-hydroxyacid... 131 7e-32 gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid... 75 9e-15 gi|300313598|ref|YP_003777690.1| D-3-phosphoglycerate dehydrogen... 67 2e-12 gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [... 67 3e-12 >gi|300310460|ref|YP_003774552.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 308 Score = 247 bits (630), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 131/290 (45%), Positives = 178/290 (61%), Gaps = 1/290 (0%) Query: 25 ARRMPELQVRLWPDLPDPDDVEAVAGWYPPAGVIERLPNLRLISSIAAGVDHVLGAARAH 84 A++ PEL ++WP D V +A W PP + ER P L ++ S+ AG+D + Sbjct: 20 AQKAPELPFQIWPHDGDGASVRYLAAWTPPDDIAERFPKLEILFSVGAGIDQ-FDMSTLP 78 Query: 85 PLVPVCRIVDPALAAGMAEYVLWAVLYYHRALDQALQQQVQRVWKIPSQKPAYQTHVGLM 144 P +PV R+ +P +AAGM +YV AVL HR +QQ +VW+ +PA VG++ Sbjct: 79 PALPVVRMTEPGIAAGMVQYVTHAVLGLHRDSRVYARQQRDQVWRAHRVRPASACRVGVL 138 Query: 145 GLGALGSHLAGVLRDQGFTVSGWSRSAHHIEGVRTYAGDDALHAFLQAPDIVVCLLPLTD 204 GLG LG + L GF +GWSRSAH ++G+ Y+G L AFL D++VCL+PLT+ Sbjct: 139 GLGMLGRAVLQQLHGLGFPCAGWSRSAHQMDGIDCYSGSAGLRAFLARTDVLVCLVPLTE 198 Query: 205 ATRKLLGRDVFAAMKPGAALVHCGRGEHLVEEDLLEALDRQHLRGAVLDVFAQEPLPASN 264 TR LL RD+FA + GAAL++ GRG HLVE+DLL AL+ L AVLDV EPLP + Sbjct: 199 ETRGLLCRDLFAQLPRGAALINVGRGGHLVEDDLLAALNDGQLSAAVLDVCEVEPLPPGH 258 Query: 265 ALWSHPRVVVTPHMASSAQPGVIVGQILDNLQRLRRGEAALHEVDRSRGY 314 W+HP +V+TPH+AS QP V +L+NL+R R G L +DR RGY Sbjct: 259 PFWTHPDIVLTPHIASMTQPDGAVDAVLENLRRYREGLPLLGLIDRQRGY 308 >gi|300311801|ref|YP_003775893.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 319 Score = 131 bits (329), Expect = 7e-32, Method: Compositional matrix adjust. Identities = 106/262 (40%), Positives = 139/262 (53%), Gaps = 1/262 (0%) Query: 53 PPAGVIERLPNLRLISSIAAGVDHVLGAARAHPLVPVCRIVDPALAAGMAEYVLWAVLYY 112 P ++R+PNL + S+ AGV+ ++ A + + R+VDP LAA MAE VL LY Sbjct: 59 PDPAQLKRMPNLLWVHSVWAGVERLV-ADLGQSDLQLTRLVDPRLAATMAEAVLAWTLYL 117 Query: 113 HRALDQALQQQVQRVWKIPSQKPAYQTHVGLMGLGALGSHLAGVLRDQGFTVSGWSRSAH 172 HR + ++Q + W+ Q V L+GLGALG A LR F V+GWSRSA Sbjct: 118 HRDMPLYARRQREHHWEAQPYVLPEQRRVSLLGLGALGEAAAAALRQARFAVNGWSRSAR 177 Query: 173 HIEGVRTYAGDDALHAFLQAPDIVVCLLPLTDATRKLLGRDVFAAMKPGAALVHCGRGEH 232 EGV+T+ G L L DI+VCLLPLT TR L+ A + P A L++ RG Sbjct: 178 EAEGVQTFHGPAGLAQMLAQTDILVCLLPLTAETRGLIDAAHLAMLPPQAQLINFARGPI 237 Query: 233 LVEEDLLEALDRQHLRGAVLDVFAQEPLPASNALWSHPRVVVTPHMASSAQPGVIVGQIL 292 + + L ALD LR AVLDVFA EPLP + WSHP V V PH ++ + Sbjct: 238 IDDAALKAALDAGQLRHAVLDVFAVEPLPPAAWPWSHPAVTVLPHCSAPTDRQTASAIVA 297 Query: 293 DNLQRLRRGEAALHEVDRSRGY 314 N++R R VD RGY Sbjct: 298 ANIRRYRHSGEIAANVDLRRGY 319 >gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 74.7 bits (182), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 75/280 (26%), Positives = 118/280 (42%), Gaps = 28/280 (10%) Query: 26 RRMPELQVRLWPDLPDPDDVEAVAGWYPPAGVI-----------ERLPNLRLISSIAAGV 74 R E + L+ + D + + +A G+I E P L+++S+I+ GV Sbjct: 18 RLQAEFDLTLFDRITDDNRADFIAALATAHGMIGASLPITNAMLEAAPQLKIVSTISVGV 77 Query: 75 DHV-LGAARAHPLVPVCRIVDPALAAGMAEYVLWAVLYYHRALDQALQQQVQRVWKIPSQ 133 D+ L R L+ L A+ + +L R + + + WK Sbjct: 78 DNFDLDYFRQRGLM--LAHTPGVLTEATADTIFALILASARRVVELAEYVKAGRWKGSIG 135 Query: 134 KPAYQTHV-----GLMGLGALGSHLAG-VLRDQGFTVSGWSR----SAHHIEGVRTYAGD 183 + + +V GL+G+G +GS +A G + +R A G R + + Sbjct: 136 EAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYHNRRPDPEAERELGARYVSKE 195 Query: 184 DALHAFLQAPDIVVCLLPLTDATRKLLGRDVFAAMKPGAALVHCGRGEHLVEEDLLEALD 243 D L D V +LPLT T ++ G FA MK A ++ RG + E L+ AL Sbjct: 196 D----LLAQADFVCVMLPLTPQTERMFGAPEFALMKRSAIFINASRGRIVDEAALIAALQ 251 Query: 244 RQHLRGAVLDVFAQEPLPASNALWSHPRVVVTPHMASSAQ 283 + + GA LDVF EPLP + L P VV PH+ S+ Sbjct: 252 DKTIHGAGLDVFEVEPLPLQSPLLKLPNVVALPHIGSATH 291 >gi|300313598|ref|YP_003777690.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 328 Score = 67.4 bits (163), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 50/147 (34%), Positives = 69/147 (46%), Gaps = 3/147 (2%) Query: 141 VGLMGLGALGSHLAGVLRDQGFTVSGWSRSAHHIEGVRT-YAGDDALHAFLQAPDIVVCL 199 +G+ G G +G +AG R G V W R + + ++ Y + F D++ Sbjct: 153 LGIWGYGKIGRLVAGYGRAFGMQVLVWGRDSSREQAIKDGYQAAASKEEFFAQADVLSLH 212 Query: 200 LPLTDATRKLLGRDVFAAMKPGAALVHCGRGEHLVEEDLLEALDRQHLRG-AVLDVFAQE 258 L L DATR ++ A MK A V+ R E LVE D LEA G A LDV+ +E Sbjct: 213 LRLNDATRGIVKASDLARMKATACFVNTSRAE-LVEPDALEAALIAGRPGLAALDVYERE 271 Query: 259 PLPASNALWSHPRVVVTPHMASSAQPG 285 PLP +AL VV PH+ + G Sbjct: 272 PLPVESALLQMDNVVAAPHLGYVEKDG 298 >gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 316 Score = 66.6 bits (161), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 66/227 (29%), Positives = 101/227 (44%), Gaps = 11/227 (4%) Query: 58 IERLPNLRLISSIAAGVDHVLGAARAHPLVPVCRIVDPALAAGMAEYVLWAVLYYHRALD 117 + R P L ++S G D V A + V D L +A+ + +L R + Sbjct: 65 MARFPALEIVSVFGVGYDGVDVPAARERGIHVTHTPD-VLNDDVADLAMALMLATARNVV 123 Query: 118 QALQQQVQRVWK---IPSQKPAYQTHVGLMGLGALGSHLAGVLRDQGFTVSGWSRSAHHI 174 +A + WK P +G++GLG +G +A +S +RS + Sbjct: 124 RADRFARSGEWKKGPFPFTTKVSGARLGIVGLGRIGQAIAKRAAAFDMQISYHNRSRKDV 183 Query: 175 EGVRTYAGDDALHAFLQAPDIVVCLLPLTDATRKLLGRDVFAAMKPGAALVHCGRGEHLV 234 Y D++ A + D +V + P TR L+ +V A+ P L++ RG + Sbjct: 184 P----YTYVDSITALAREVDFLVMITPGGAGTRALVNAEVLEALGPKGFLINVARGSVVD 239 Query: 235 EEDLLEALDRQHLRGAVLDVFAQEP-LPASNALWSHPRVVVTPHMAS 280 E L+ AL + GA LDVFA EP +PA L + VV+TPHMAS Sbjct: 240 EAALIAALKTGVIAGAGLDVFADEPNVPAE--LAALDNVVLTPHMAS 284 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,146,158 Number of extensions: 53998 Number of successful extensions: 192 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 183 Number of HSP's successfully gapped: 11 Length of query: 314 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 229 Effective length of database: 1,217,395 Effective search space: 278783455 Effective search space used: 278783455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)