BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0077.p (293 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312237|ref|YP_003776329.1| amino acid ABC transporter perm... 557 e-160 gi|300310992|ref|YP_003775084.1| branched-chain aminoacid ABC tr... 175 5e-45 gi|300310521|ref|YP_003774613.1| branched-chain amino acid ABC t... 167 1e-42 gi|300309519|ref|YP_003773611.1| branched-chain amino acid ABC t... 167 1e-42 gi|300314070|ref|YP_003778162.1| branched-chain amino acid ABC t... 145 3e-36 >gi|300312237|ref|YP_003776329.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 293 Score = 557 bits (1436), Expect = e-160, Method: Compositional matrix adjust. Identities = 290/293 (98%), Positives = 292/293 (99%) Query: 1 MLEQQLINALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLLVEQMALPLW 60 MLEQQLINALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLLVEQMALPLW Sbjct: 1 MLEQQLINALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLLVEQMALPLW 60 Query: 61 VALLLAMVATGTLGLIIDVLVLKPLRKRNAPHLIPMIATIGVAIMITNISQGIFGAENKR 120 VALLLAMVATG LGLIIDVLVLKPLRKRNAPHLIPMIATIGVAIMITNISQG+FGAENKR Sbjct: 61 VALLLAMVATGLLGLIIDVLVLKPLRKRNAPHLIPMIATIGVAIMITNISQGLFGAENKR 120 Query: 121 FPQGTIPEDSVSLGNLHITAVQVAIIVIAFVLMVVLLGVMRKTQLGRALRAIAESPKAAY 180 FPQGTIPE+SVSLGNLHITAVQVAIIVIAFVLMVVLLGVMRKTQLGRALRAIAESPKAAY Sbjct: 121 FPQGTIPEESVSLGNLHITAVQVAIIVIAFVLMVVLLGVMRKTQLGRALRAIAESPKAAY 180 Query: 181 LLGINVEGLFLLTSFAAAALGGAAGVLVGVSFNAISPFMGQPMLHKGIAVIILGGMGDIR 240 LLGINVEGLFLLTSFAAAALGGAAGVLVGVSFNAISPFMGQPMLHKGIAVIILGGMGDIR Sbjct: 181 LLGINVEGLFLLTSFAAAALGGAAGVLVGVSFNAISPFMGQPMLHKGIAVIILGGMGDIR 240 Query: 241 GAMIGGLFLGFAEVLTVAYISSDFRDAVAFGLLFLILLVKPSGMFGKVLERKA 293 GAMIGGLFLGFAEVLTVAYISSDFRDAVAFGLLFLILLVKPSGMFGKVLERKA Sbjct: 241 GAMIGGLFLGFAEVLTVAYISSDFRDAVAFGLLFLILLVKPSGMFGKVLERKA 293 >gi|300310992|ref|YP_003775084.1| branched-chain aminoacid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 308 Score = 175 bits (443), Expect = 5e-45, Method: Compositional matrix adjust. Identities = 108/297 (36%), Positives = 173/297 (58%), Gaps = 13/297 (4%) Query: 2 LEQQLINALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLLVEQMALP--- 58 L QQL+N LSLG++YAL A+G+T+V+G++G+IN +HG I+M+ SY L+ + + + Sbjct: 8 LTQQLVNGLSLGAIYALIAIGYTMVYGIIGMINFAHGEIYMIASYVGLVTLTAIGVQSGY 67 Query: 59 -----LWVALLLAMVATGTLGLIIDVLVLKPLRKRNAPHLIPMIATIGVAIMITNISQGI 113 L AL+++++ TG G ++ + +PLR P L+P+I+ IG++I + N Q Sbjct: 68 PLPLLLGGALIVSVLVTGLYGWTVERVAYRPLR--GGPRLVPLISAIGMSIFLQNYVQIG 125 Query: 114 FGAENKRFP---QGTIPEDSVSLGNLHITAVQVAIIVIAFVLMVVLLGVMRKTQLGRALR 170 GA + P G + S + I ++ I+ + VLMV L + ++++GRA R Sbjct: 126 QGARDMSVPVLISGALEFQMGSDFTVTIPYSRMVIVGVTLVLMVALTLFIARSRMGRACR 185 Query: 171 AIAESPKAAYLLGINVEGLFLLTSFAAAALGGAAGVLVGVSFNAISPFMGQPMLHKGIAV 230 A AE A LLGI+ + T A L GVL+ ++ ++P++G K Sbjct: 186 ACAEDMGMANLLGIDTNKVISFTFVLGAMLAAVGGVLIALTIGKLNPYIGFIAGIKAFTA 245 Query: 231 IILGGMGDIRGAMIGGLFLGFAEVLTVAYISSDFRDAVAFGLLFLILLVKPSGMFGK 287 +LGG+G I GAM+GG+ LG AE Y+ S+++D V+FGLL LILL +P+G+ GK Sbjct: 246 AVLGGIGSIPGAMLGGVLLGLAETFAAGYLPSEYKDVVSFGLLVLILLFRPTGLLGK 302 >gi|300310521|ref|YP_003774613.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 309 Score = 167 bits (423), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 114/307 (37%), Positives = 186/307 (60%), Gaps = 20/307 (6%) Query: 4 QQLINALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLL---VEQMA--LP 58 QQ+IN L LGS+YAL ALG+T+V+GVL +IN +HG I M+G+ L L V+Q+A LP Sbjct: 6 QQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQVAPGLP 65 Query: 59 LWVALLLAMVATGTLGLIIDVLV----LKPLRKRNAPHLIPMIATIGVAIMITNISQGIF 114 V L++A+V + +++ +L+ +PLR NAP L P+I IGV+I++ ++ I+ Sbjct: 66 GIVQLVIAIVGAIPVCIVVSLLIERIAYRPLR--NAPRLAPLITAIGVSILLQTLAMMIW 123 Query: 115 GAENKRFPQGTIPEDSVSLGNLHITAVQVAIIVIAFVLMVVLLGVMRKTQLGRALRAIAE 174 G FPQ +P D V + I+ Q+ ++ +A + MV L+ ++ KT++GRA+RA AE Sbjct: 124 GRSPLPFPQ-VMPSDPVHIAGALISPTQIMLLALAVLAMVGLVLIVEKTKMGRAMRATAE 182 Query: 175 SPKAAYLLGINVEGLFLLTSFAAAALGGAAGVLVGVSFNAISPFMGQPMLHKGIAVIILG 234 +P+ A L+G++ + ++T A L AGV+ +++ MG K + +LG Sbjct: 183 NPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAVLG 242 Query: 235 GMGDIRGAMIGGLFLGFAEVLTVAYI--------SSDFRDAVAFGLLFLILLVKPSGMFG 286 G+G+I GAM+GG+ LG E L YI S+++D AF +L ++L ++PSG+ G Sbjct: 243 GIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLTLRPSGIMG 302 Query: 287 KVLERKA 293 + + +A Sbjct: 303 ERVADRA 309 >gi|300309519|ref|YP_003773611.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 309 Score = 167 bits (423), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 114/307 (37%), Positives = 186/307 (60%), Gaps = 20/307 (6%) Query: 4 QQLINALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLL---VEQMA--LP 58 QQ+IN L LGS+YAL ALG+T+V+GVL +IN +HG I M+G+ L L V+Q+A LP Sbjct: 6 QQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQVAPGLP 65 Query: 59 LWVALLLAMVATGTLGLIIDVLV----LKPLRKRNAPHLIPMIATIGVAIMITNISQGIF 114 V L++A+V + +++ +L+ +PLR NAP L P+I IGV+I++ ++ I+ Sbjct: 66 GIVQLVIAIVGAIPVCIVVSLLIERIAYRPLR--NAPRLAPLITAIGVSILLQTLAMMIW 123 Query: 115 GAENKRFPQGTIPEDSVSLGNLHITAVQVAIIVIAFVLMVVLLGVMRKTQLGRALRAIAE 174 G FPQ +P D V + I+ Q+ ++ +A + MV L+ ++ KT++GRA+RA AE Sbjct: 124 GRSPLPFPQ-VMPSDPVHIAGALISPTQIMLLALAVLAMVGLVLIVEKTKMGRAMRATAE 182 Query: 175 SPKAAYLLGINVEGLFLLTSFAAAALGGAAGVLVGVSFNAISPFMGQPMLHKGIAVIILG 234 +P+ A L+G++ + ++T A L AGV+ +++ MG K + +LG Sbjct: 183 NPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAVLG 242 Query: 235 GMGDIRGAMIGGLFLGFAEVLTVAYI--------SSDFRDAVAFGLLFLILLVKPSGMFG 286 G+G+I GAM+GG+ LG E L YI S+++D AF +L ++L ++PSG+ G Sbjct: 243 GIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLTLRPSGIMG 302 Query: 287 KVLERKA 293 + + +A Sbjct: 303 ERVADRA 309 >gi|300314070|ref|YP_003778162.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 294 Score = 145 bits (367), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 93/275 (33%), Positives = 157/275 (57%), Gaps = 4/275 (1%) Query: 13 GSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLLVEQMALPLWVALLLAMVATGT 72 GS YA+ +LG ++FG+L VIN +HG ++MLG++ A + + + L WV L++A + G Sbjct: 22 GSFYAMLSLGLAVIFGLLNVINFAHGTLYMLGAFLAWMGLSYLGLNYWVMLIVAPILVGA 81 Query: 73 LGLIIDVLVLKPLRKRNAPHLIPMIATIGVAIMITNISQGIFGAENKRFPQGTIPEDSVS 132 G++I+ +L+ L K + HL ++ T G+ +MI I + I+G + + + + + + Sbjct: 82 FGIVIEKTMLRWLYKLD--HLYGLLLTFGITLMIEGIFRSIYGVSGQPYSVPDLLQGATN 139 Query: 133 LGNLHITAVQVAIIVIAFVLMVVLLGVMRKTQLGRALRAIAESPKAAYLLGINVEGLFLL 192 LG + + + ++V + ++ V+ KT+LG LRA E+PK GINV + L Sbjct: 140 LGFMVLPNYRAWVVVASLIVCFATWFVIEKTRLGAYLRAGTENPKLVEAFGINVPLMVTL 199 Query: 193 TSFAAAALGGAAGVLVGVSFNAISPFMGQPMLHKGIAVIILGGMGDIRGAMIGGLFLGFA 252 T AL AGVL +SP MG ++ AV+++GGMG I G++I GL LG Sbjct: 200 TYGFGVALAAFAGVLAAPVIQ-VSPLMGSNLIITVFAVVVIGGMGSILGSIITGLVLGLI 258 Query: 253 EVLTVAYISSDFRDAVAFGLLFLILLVKPSGMFGK 287 E LT + + V F ++ ++L+V+P+G+FGK Sbjct: 259 EGLTRVF-YPELSATVVFIIMAIVLMVRPAGLFGK 292 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.145 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 783,581 Number of extensions: 29523 Number of successful extensions: 299 Number of sequences better than 1.0e-08: 35 Number of HSP's gapped: 248 Number of HSP's successfully gapped: 35 Length of query: 293 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 209 Effective length of database: 1,222,130 Effective search space: 255425170 Effective search space used: 255425170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)