BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0076.p (380 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312236|ref|YP_003776328.1| amino acid ABC transporter peri... 739 0.0 gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC t... 119 5e-28 gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC t... 104 1e-23 gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC t... 97 2e-21 gi|300312955|ref|YP_003777047.1| amino acid ABC transporter peri... 94 2e-20 >gi|300312236|ref|YP_003776328.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 739 bits (1909), Expect = 0.0, Method: Compositional matrix adjust. Identities = 372/380 (97%), Positives = 377/380 (99%) Query: 1 MLKTIPALLIGATLSSAALAADIKLGVAEALTGPAAKYGVAIKNGFTLASEEINAKGGVN 60 MLKTIPALLIGATLS AALAADIKLGVAEALTGPAAKYGVAIKNGFTLASEEINAKGGVN Sbjct: 6 MLKTIPALLIGATLSQAALAADIKLGVAEALTGPAAKYGVAIKNGFTLASEEINAKGGVN 65 Query: 61 GDKLALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKVVVFGT 120 GDKLALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKVVVFGT Sbjct: 66 GDKLALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKVVVFGT 125 Query: 121 SNTADGITAMGPFTFRNSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFTKSGYDVFK 180 SNTADGITAMGPFTFRNSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFTKSGYDVFK Sbjct: 126 SNTADGITAMGPFTFRNSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFTKSGYDVFK 185 Query: 181 ATLEQQKIPVTTTEAYAKGDVDFKAQLTKIKAGNPDAIVCSCLAEEAANIILQTRALGMK 240 ATLE QKIPVTTTEAYAKGDVDFKAQLTKIKAGNPDAIVCSCLAEEAANIILQTRALGMK Sbjct: 186 ATLEHQKIPVTTTEAYAKGDVDFKAQLTKIKAGNPDAIVCSCLAEEAANIILQTRALGMK 245 Query: 241 QPFIGGNGLNSPKLFEIAKDAGDNTLMGSPWSAENQTPANKAFIAAYKAKFGSDPDQFAA 300 QPFIGGNGLNSPKLFEIAKDAGDNTLMGSPWSAENQTPANKAFI AYKAKFG+DPDQFAA Sbjct: 246 QPFIGGNGLNSPKLFEIAKDAGDNTLMGSPWSAENQTPANKAFITAYKAKFGADPDQFAA 305 Query: 301 QAYDAMYIMADAIKSVKLSGNLAKDRDAVRAALPAVKIDGATGKFAFRAAPAVAGKQVGF 360 QAYDAMYI+ADA+K+VKLSGNLAKDRDA+RAALPAVKIDGATGKFAFRAAPAVAGKQVGF Sbjct: 306 QAYDAMYIVADALKNVKLSGNLAKDRDALRAALPAVKIDGATGKFAFRAAPAVAGKQVGF 365 Query: 361 DADQEAIVNIAKGGKFVLLK 380 DADQEAIVNIAKGGKFVLLK Sbjct: 366 DADQEAIVNIAKGGKFVLLK 385 >gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 383 Score = 119 bits (297), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 106/351 (30%), Positives = 162/351 (46%), Gaps = 20/351 (5%) Query: 9 LIGATLSSAAL---AAD-IKLGVAEALTGPAAKYGVAIKNGFTLASEEINAKGGVNGDKL 64 + A L S AL AAD IK+GV+ TG +A GV++++G LA EINA GG+ G ++ Sbjct: 8 FVAAALMSLALTSQAADPIKIGVSGPYTGGSAPMGVSMRDGVKLAVAEINAAGGLLGRQI 67 Query: 65 ALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKVVVFGTSNTA 124 L+ D++ K E + + ++LI ++KV+A G + A A+ AK+ V T Sbjct: 68 QLIERDDEAKNERGVQIAQELINKEKVVATVGFINTGVALASQRFYQEAKIPVINNVATG 127 Query: 125 DGITAM-----GPFTFRNSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFTKSGYDVF 179 IT + FR S + + AV KVA++ + + + G + Sbjct: 128 TVITKQFEKEKDNYVFRTSAADKIQAKMIADEAVDKQKFTKVAIL-ADSTNYGQLGREDL 186 Query: 180 KATLEQQKIPVTTTEAYAKGDVDFKAQLTKIKAGNPDAIVCSCLAEEAANIILQTRALGM 239 + L +K+ E Y DVD AQL K K G A++ + E A I L Sbjct: 187 EKVLADKKVTPVAVEKYNIKDVDMTAQLLKSKQGGAQAVLTYGIGPELAQIANGMEKLNW 246 Query: 240 KQPFIGGNGLNSPKLFEIAKDAGDNTLMGSPWSAENQTPANKAFIAAYKAKFGSD--PDQ 297 P IG L+ + + G+ M + + TP KAFI AY+ + D P Sbjct: 247 HVPIIGSWPLSMGNFIDNSGKNGNGARMPQTFIQDGNTPKRKAFIEAYQKAYKVDRMPSA 306 Query: 298 F-AAQAYDAMYIMADAIKSVKLSGNLAKDRDAVRAALPAV--KIDGATGKF 345 AAQ YD++ ++A AIK + D D VRAAL + K++G + Sbjct: 307 VSAAQGYDSILLLAAAIKQAGTT-----DGDKVRAALENLNTKVEGVVTTY 352 >gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 373 Score = 104 bits (260), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 94/303 (31%), Positives = 145/303 (47%), Gaps = 11/303 (3%) Query: 20 AADIKLGVAEALTGPAAKYGVAIKNGFTLASEEINAKGGVNGDKLALVIEDEQGKKEEAI 79 A +K+ +A +TG A+YG +K G A E+INA GG NG+K V+ D+ + ++A+ Sbjct: 26 AETVKIAIAGPMTGAVAQYGDMVKAGALTAIEQINAAGGANGNKFEAVMMDDACEPKQAV 85 Query: 80 NVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKVVVFGTSNTADGITAMGP--FTFRN 137 V K++ Q + V G S S A I VV+ S TA +T P F FR Sbjct: 86 AVANKIVSQG-IKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTEAKPHKFIFRT 144 Query: 138 SVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFTKSGYDVFKATLEQQKIPVTTTEAYA 197 + P + ++ KKVAV++ + ++ + KA L+ K+PV E Sbjct: 145 IGRDDQQGPAAARYVIEKLKPKKVAVLH-DKQSYGQGVASSVKAALDAAKVPVVVFEGIN 203 Query: 198 KGDVDFKAQLTKIKAGNPDAIVCSCLAEEAANIILQTRALGMKQPFIGGNGLNSPKLFEI 257 GD D+ A +TK+K+ D + E I+ Q R G+K F+G G+ + + I Sbjct: 204 AGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQAREQGIKAVFMGPEGVGNKDITAI 263 Query: 258 AKDAGDNTLMGSP--WSAENQTPANKAFIAAYKAKFGSDPD-QFAAQAYDAMYIMADAIK 314 A A + L+ P ++A+ PAN A + A DP+ F AY + I+ADAI Sbjct: 264 AGPASEGMLVTLPADFAAD---PANAA-LVKAFAAAKRDPNGPFQMPAYSGVKIIADAIA 319 Query: 315 SVK 317 K Sbjct: 320 GAK 322 >gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 378 Score = 97.1 bits (240), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 104/363 (28%), Positives = 165/363 (45%), Gaps = 24/363 (6%) Query: 1 MLKTIPAL-LIGATLS---SAALAAD---IKLGVAEALTGPAAKYGVAIKNGFTLASEEI 53 M K + L +I A+L+ + A+A + +K+G + LTGP A G + G +A E + Sbjct: 1 MFKRVSGLNIIAASLALIPAFAMAQETQVVKIGFSSPLTGPQASAGKDNQGGLMMAIERL 60 Query: 54 NAKG-GVNGDKLA--LVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIA 110 NA+ V G K+ ++ ED+Q + + V +KL Q V A+ GP S A + Sbjct: 61 NAQPITVGGKKIKFDVIAEDDQADPKSGVAVAQKLADQG-VKAIVGPYNSGVTIPASRVY 119 Query: 111 NAAKVVVFGTSNTADGITAMGPFT-FRNSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDD 169 N A +VV T + IT G T FR + ++ + A K K+VAVI DD Sbjct: 120 NDAGIVV-ATVASNPKITQQGFATLFRVAASDSQLGGKMALYAAKELKFKRVAVI---DD 175 Query: 170 --AFTKSGYDVFKATLEQQKIPVTTTEAYAKGDVDFKAQLTKIKAGNPDAIVCSCLAEEA 227 A+ + F + I V +T+ DF A LT IK PDA+ A + Sbjct: 176 RTAYGQGLAQEFIKVAKANGIDVVSTDFTNDKATDFTAILTSIKGKKPDAVFLGGYAPQG 235 Query: 228 ANIILQTRALGMKQPFIGGNGLNSPKLFEIAKDA-GDNTLMGSPWSAENQTPANKAFIAA 286 I Q + LG+ P +GG+G+ SP++ + DA G++ + ++ K F Sbjct: 236 GPIKRQMKQLGVDVPLMGGDGICSPEMGRLGGDAIGESVYCTQGGTMLDKAKEGKVFSDE 295 Query: 287 YKAKFGSDPDQFAAQAYDAMYIMADAIKSVKLSGNLAKDRDAVRAALPAVKIDGATGKFA 346 Y+ K+ + +A YD M ++A A+K + D AL + G G++ Sbjct: 296 YQKKYNRPAETYAVSFYDGMMLIAQAMKQAN-----SVDPKQFGPALAKISYKGVAGQYD 350 Query: 347 FRA 349 F A Sbjct: 351 FDA 353 >gi|300312955|ref|YP_003777047.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 392 Score = 94.0 bits (232), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 105/360 (29%), Positives = 163/360 (45%), Gaps = 42/360 (11%) Query: 7 ALLIGATLSSAALAADI-KLGVAEALTGPAAKYGVAIKNGFTLASEEI-NAKGGVNGDKL 64 A+ +GA+L A AAD+ K+G L+GPA+ GV + GF LA + + N GGV + Sbjct: 14 AMAVGASLP--AQAADVVKVGFLTTLSGPASAPGVDEREGFMLALKTLGNKLGGVPAE-- 69 Query: 65 ALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKVVVFGTSNTA 124 +++ D+Q + A +LI ++KV + G SN A P A A+K VV + Sbjct: 70 -VIVVDDQFSPDAAKQGADRLIKREKVDILSGFVYSNILLAVAPAAFASKTVVVSANAGP 128 Query: 125 DGITAMG--PFTFRNSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFTKSGYDVFKAT 182 + G P+ F S + D + G ++VA+I N A K FK Sbjct: 129 AQLAGAGCNPYFFSAS-YQNDAAHEAAGKLMADKGYQRVAIIAPNYPA-GKDASAGFKRF 186 Query: 183 LEQQKIPVTTTEAYAK-GDVDFKAQLTKIKAGNPDAIVCSCLAEEAANIILQTRALGMKQ 241 L++ E + K G +DF ++ +++A N DA+ N + Q A GM Sbjct: 187 LKKD----PAVELFPKLGQLDFATEIAQLRAANIDALYFFLPGGMGVNFVKQYAAAGM-- 240 Query: 242 PFIGGNGLNSPKLFEI--AKDAGDNTLMGS----------PWSAENQTPANKAFIAAYKA 289 PK+ + A DA L WS++ N+AF+AAY Sbjct: 241 ---------DPKIVRVMPAYDADQQMLSNGDLIAGVANTGQWSSDLPNEQNRAFVAAYLK 291 Query: 290 KFGSDPDQFAAQAYDAMYIMADAIKSVKLSGNLAKDRDAVRAALPAVKIDGATGKFAFRA 349 ++G P FA+QAYDA ++ A+K+V G ++R+A+ AA+ G F F A Sbjct: 292 EYGRQPSMFASQAYDAAMLIDSAVKAV---GGKVENREALIAAIKTANFKSVRGGFRFAA 348 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.132 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,135,013 Number of extensions: 46771 Number of successful extensions: 245 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 220 Number of HSP's successfully gapped: 19 Length of query: 380 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 293 Effective length of database: 1,207,925 Effective search space: 353922025 Effective search space used: 353922025 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)