BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0071.p (276 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312232|ref|YP_003776324.1| signal transduction protein [He... 513 e-147 gi|300311938|ref|YP_003776030.1| hypothetical protein Hsero_2626... 239 2e-64 gi|300311595|ref|YP_003775687.1| two component response regulato... 81 7e-17 gi|300310564|ref|YP_003774656.1| regulator of the two-component ... 72 5e-14 >gi|300312232|ref|YP_003776324.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 274 Score = 513 bits (1322), Expect = e-147, Method: Compositional matrix adjust. Identities = 250/276 (90%), Positives = 266/276 (96%), Gaps = 2/276 (0%) Query: 1 MNLEALFQQQTALPSIPKVVQEVIESFNNESVSIEEIAKKLAADQVLSAKILRLANSSYY 60 MNLEALFQQQTALPSIPKVVQEVIESFNNESVSIEEIAKKLAADQVLSAKILRLANSSYY Sbjct: 1 MNLEALFQQQTALPSIPKVVQEVIESFNNESVSIEEIAKKLAADQVLSAKILRLANSSYY 60 Query: 61 HVSRTIGTVDDAVLMLGFMTVRTLVVSTGMAGSFKKLPGVDLKLFWRYSLDTAIVAKWLA 120 HVSRTIGTVDDAVLMLGFMTVRTLVVSTGMAGSFKKLPGVDLKLFWRYSLDTA+VAKWLA Sbjct: 61 HVSRTIGTVDDAVLMLGFMTVRTLVVSTGMAGSFKKLPGVDLKLFWRYSLDTALVAKWLA 120 Query: 121 KQKQVKLNSDFAFTVGLMHAIGQLVMHAGMPEQALEVDKVAGPLDARRLDVERQSFGYDF 180 K+ KLNSDFAFTVGLMHAIGQLVMHAGMPEQAL+VDKVAGPLDARRLDVER+SFGYDF Sbjct: 121 KRG--KLNSDFAFTVGLMHAIGQLVMHAGMPEQALQVDKVAGPLDARRLDVERKSFGYDF 178 Query: 181 ADVGAELARRWRFPDEFAKVIKAFPQPLKGEFDPFAAVVHLAAWRARAEENKYSADELES 240 ADVGAELARRWRFPDEFAKVI+AFPQPL+GEFDPFAA+VHLAAWRARA EN+YS +EL + Sbjct: 179 ADVGAELARRWRFPDEFAKVIEAFPQPLQGEFDPFAAIVHLAAWRARAHENQYSDEELAA 238 Query: 241 TFPAEVAAKLGLSLSTFTEDMPPLAELGEGMQDLIS 276 TFPAEVA +LG+ L TFT++MPPL ELGEGMQ+LI+ Sbjct: 239 TFPAEVAERLGVRLETFTQEMPPLEELGEGMQELIA 274 >gi|300311938|ref|YP_003776030.1| hypothetical protein Hsero_2626 [Herbaspirillum seropedicae SmR1] Length = 290 Score = 239 bits (609), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 126/289 (43%), Positives = 177/289 (61%), Gaps = 14/289 (4%) Query: 1 MNLEALFQQQTALPSIPKVVQEVIESFNNESVSIEEIAKKLAADQVLSAKILRLANSSYY 60 M L++L Q LP++PKVVQ+++ SFN E +S EIA+++A D LSAK+LRLANS+++ Sbjct: 1 MELKSLVDQPGKLPTVPKVVQQLMASFNAEDISAHEIAQQIATDPALSAKVLRLANSAFF 60 Query: 61 HVSRTIGTVDDAVLMLGFMTVRTLVVSTGMAGSFKKLPGVDLKLFWRYSLDTAIVAKWLA 120 H SRTIGTVDDA+ +LGF+ VR LV+ GM +FK G+DL FWR++L TA+ A+WLA Sbjct: 61 HASRTIGTVDDALRILGFIMVRNLVLGNGMVAAFKNTRGMDLHQFWRHTLYTAVAARWLA 120 Query: 121 KQKQV--KLNSDFAFTVGLMHAIGQLVMHAGMPEQALEVDKVAGPLDARRLDVERQSFGY 178 + + N D FTVG+MH IGQL +HA +D+ + LD+ R + E++++G+ Sbjct: 121 MHAGMVGEDNGDAVFTVGMMHGIGQLQLHAVAANAVAPLDRHSNVLDSDRAEREKEAWGF 180 Query: 179 DFADVGAELARRWRFPDEFAKVIKAFPQPL-KGEFDPFAAVVHLAAWRARAEENKYSADE 237 +ADV AELA W FPD +++ P PL G FD AA VHL W AR + Sbjct: 181 HYADVSAELAAIWHFPDALVTALRSVPDPLGAGRFDRAAAWVHLGGWAARVAILSQDDEN 240 Query: 238 LESTFPAEVAAKLGLS------LSTFTED-----MPPLAELGEGMQDLI 275 ++T+PA V KLG+ L D MP L EL G+ ++ Sbjct: 241 AQATYPAAVGKKLGVDPAWMPLLVKHDPDKAERAMPSLGELAAGLDAML 289 >gi|300311595|ref|YP_003775687.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 310 Score = 81.3 bits (199), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 52/198 (26%), Positives = 101/198 (51%), Gaps = 5/198 (2%) Query: 7 FQQQTALPSIPKVVQEVIESFNNESVSIEEIAKKLAADQVLSAKILRLANSSYYHVSRTI 66 Q LP+ V ++++ E +++ E+ ++ D VL+ +++++AN + R + Sbjct: 13 MQDSEVLPAPSGVRLKIMQMCQQEDIALPELVAQVQGDPVLAGRLIQIANIPNINKGRMV 72 Query: 67 GTVD-DAVLMLGFMTVRTLVVSTGMAGSFKK--LPGVDLKLFWRYSLDTAIVAKWLAKQK 123 +V+ + +LM+G VR + ++ + S ++ D FW S A + L Sbjct: 73 ASVNVELLLMVGLHAVRQMALAISLTESARRGDCAEFDYDAFWSRSTAMACAGQALGDLL 132 Query: 124 QVKLNSDFAFTVGLMHAIGQLVMHAGMPEQALEVDKVAGPLDARRLDVERQSFGYDFADV 183 QV ++ FT+GL+ IGQL + + P++ E+ +AG DA+ ER+SFG+D + Sbjct: 133 QVAPPAEM-FTIGLLAGIGQLGLASARPKRYGEL-LLAGAKDAQLRVQERESFGFDHLAL 190 Query: 184 GAELARRWRFPDEFAKVI 201 A + + W FP F + + Sbjct: 191 AAAMMQDWHFPRIFCEAV 208 >gi|300310564|ref|YP_003774656.1| regulator of the two-component system protein [Herbaspirillum seropedicae SmR1] Length = 317 Score = 72.0 bits (175), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 61/222 (27%), Positives = 103/222 (46%), Gaps = 15/222 (6%) Query: 12 ALPSIPKVVQEVIESFNNESVSIEEIAKKLAADQVLSAKILRLANSSYYHVSRTIGTVD- 70 ALPS V ++ ++ + E+ + AD LS +I+R+ N R I +V Sbjct: 18 ALPSPGGVRLTIMRLCAQDNPDMAELIHLVQADPALSGRIIRIVNVLNAQRVRPIASVSM 77 Query: 71 DAVLMLGFMTVRTLVVSTGMAGSFKKLPGVDLKL--FWRYSLDTAIVAKWLAKQKQVKLN 128 D++L++G VR L ++ +A + + L FW +S A A+ + Q +V Sbjct: 78 DSLLLVGLQAVRQLALALSLAETRAPASCANFSLPAFWAHSFAMACSAQAVTSQLRVAPL 137 Query: 129 SDFAFTVGLMHAIGQLVMHAGMPE--QALEVDKVAGPLDARRLDVERQSFGYDFADVGAE 186 + FT GL+ IG+L + + P AL + P ARR +E+ FG++ ++ A Sbjct: 138 GEM-FTTGLLANIGKLTLASAQPTGYSALLTRALDAP--ARR-AMEQSLFGFNHLNIAAA 193 Query: 187 LARRWRFPDEFAKVIKAFPQPLK-GEF-----DPFAAVVHLA 222 L + W+ PD F + + P+ GE D +HLA Sbjct: 194 LMQDWKIPDLFCDAVLHYETPVVIGEIAATRQDEIVWTLHLA 235 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 821,220 Number of extensions: 33129 Number of successful extensions: 104 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 98 Number of HSP's successfully gapped: 4 Length of query: 276 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 192 Effective length of database: 1,222,130 Effective search space: 234648960 Effective search space used: 234648960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)