BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0063.p (158 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312226|ref|YP_003776318.1| porphyrin biosynthesis protein ... 246 4e-67 >gi|300312226|ref|YP_003776318.1| porphyrin biosynthesis protein [Herbaspirillum seropedicae SmR1] Length = 399 Score = 246 bits (629), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 123/127 (96%), Positives = 124/127 (97%) Query: 1 MKILLWLLTLFASAIGLAVLARFNPGNVVLFYPPYRIDLSLNFFIFAVLAVFLTIYVVIR 60 MKILLWLLTLFASAIGLAVLARFNPGNVVLFYPPYRIDLSLNFFIFAVLAVFL IYVVIR Sbjct: 1 MKILLWLLTLFASAIGLAVLARFNPGNVVLFYPPYRIDLSLNFFIFAVLAVFLVIYVVIR 60 Query: 61 AIRLTQKLPGRVIAYRRAKRENESNKALRDALKAYFEGRFGQAEKSATRAADLPDNAGIA 120 AIRLTQKLPGRVIAYRRAKRENESNKALRDALKAYFEGRFGQAEKSATRA+DLPDNAGIA Sbjct: 61 AIRLTQKLPGRVIAYRRAKRENESNKALRDALKAYFEGRFGQAEKSATRASDLPDNAGIA 120 Query: 121 ALIAAXA 127 ALI A A Sbjct: 121 ALIGARA 127 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.140 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 406,322 Number of extensions: 12770 Number of successful extensions: 73 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 73 Number of HSP's successfully gapped: 1 Length of query: 158 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 80 Effective length of database: 1,250,540 Effective search space: 100043200 Effective search space used: 100043200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 127 (53.5 bits)