BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0056.p (503 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312220|ref|YP_003776312.1| methyl-accepting chemotaxis tra... 622 e-179 gi|300312219|ref|YP_003776311.1| methyl-accepting chemotaxis tra... 486 e-138 gi|300313312|ref|YP_003777404.1| methyl-accepting chemotaxis pro... 457 e-130 gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis tra... 375 e-105 gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 359 e-100 >gi|300312220|ref|YP_003776312.1| methyl-accepting chemotaxis transducer transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 535 Score = 622 bits (1603), Expect = e-179, Method: Compositional matrix adjust. Identities = 334/469 (71%), Positives = 390/469 (83%) Query: 1 MTIANKLRLLLLAVVLGLASLTGLSIYHSNRIADAASYSAVNTVPSLLVLGEAGEAVFRI 60 MTIA KL LL LAV+LGLASL+ L IY S +A AASYS+VNTVPSLL LG+AGEA F I Sbjct: 1 MTIAKKLHLLTLAVILGLASLSLLGIYQSREVAKAASYSSVNTVPSLLALGDAGEAAFGI 60 Query: 61 RVGIWRYAAATDAATRAKLEKSMNTNRDKVIAAFDRYEKEDLSDATDRQLLQADRDAFAD 120 RVGIW+YAAATD +R +LEK+M D+V+AAFDRY +ED+SD TDR+LL+ADR AFAD Sbjct: 61 RVGIWKYAAATDPVSRTRLEKAMAEQHDRVLAAFDRYAREDISDDTDRKLLEADRQAFAD 120 Query: 121 YEKSRQAVIALGAEGKTEEALHAVEHQVVPPGDKLINALAAHRRYNEQLGQQGVQTAQSS 180 YEK R V+ +GAE + EEAL A+E VVPPGDKLI ALA HR+Y EQLG+QGV+TA+ Sbjct: 121 YEKQRAVVLRIGAESRNEEALRAIEQLVVPPGDKLIAALAEHRKYKEQLGRQGVETAEGI 180 Query: 181 LRSAVVQAVLMSLAVCAVVAVVSLLISRTISTALGEAIMTARAIATGDLSTRIRRRANDE 240 L+S+ QA+L+SL V A+V SLLISR IS +L +AI A AIA GDLS++++ DE Sbjct: 181 LQSSTYQAILVSLLVAAIVGATSLLISRGISVSLDDAIRVANAIAHGDLSSKVKMTGKDE 240 Query: 241 VGQLMQAIQQMSDSLVRIVSNVRNSANTIATATAEIAAGNLDLSARTEAQAGSIEETASA 300 VGQL+ AI +M+ SLV IV NVR S I +A++EIA+GNLDLSARTEAQAGSIEETASA Sbjct: 241 VGQLLHAIGEMTASLVNIVGNVRQSVAAIESASSEIASGNLDLSARTEAQAGSIEETASA 300 Query: 301 MEQLTATVRQNADNAQQANVLATSASSVATEGGQAVQQVVQTMDEINASSLRIVDIISVI 360 MEQLTATV+QNADNA QA+ LA+SASSVA+EGG+ VQ+VV TMD IN+SSL+IVDIISVI Sbjct: 301 MEQLTATVKQNADNAHQADTLASSASSVASEGGKVVQEVVHTMDAINSSSLKIVDIISVI 360 Query: 361 DGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRSLAQRSAAAAHEIKELIDDSVGKV 420 DGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSA+AA EIKELIDDSV KV Sbjct: 361 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAREIKELIDDSVEKV 420 Query: 421 ESGSRLVAQAGQTMQHVVDSVRRVTDIVADITAASAEQSDGIVQVNQAI 469 +SGSRLV QAG TM+ VV SV+RV+DIV DI+ AS EQSDGI QVNQAI Sbjct: 421 QSGSRLVMQAGNTMEQVVTSVQRVSDIVRDISVASGEQSDGIAQVNQAI 469 >gi|300312219|ref|YP_003776311.1| methyl-accepting chemotaxis transducer transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 563 Score = 486 bits (1250), Expect = e-138, Method: Compositional matrix adjust. Identities = 272/470 (57%), Positives = 345/470 (73%), Gaps = 4/470 (0%) Query: 1 MTIANKLRLLLLAVVLGLASLTGLSIYHSNRIADAASYSAVNTVPSLLVLGEAGEAVFRI 60 MTIA KL LL++AVVLG+ + +Y S+RIA +A YSA +T+PS+L L A +A++ I Sbjct: 31 MTIARKLYLLIVAVVLGVIMQAVVGLYQSHRIASSAGYSATDTIPSVLALNGATDAIYGI 90 Query: 61 RVGIWRYAAATDAATRAKLEKSMNTNRDKVIAAFDRYEKEDLSDATDRQLLQADRDAFAD 120 R+ I RY + R K +M K FD++E+ +SD TDR++L+ADR FA+ Sbjct: 91 RLNIARYV--IEPTAREKTVANMAALHQKAQDFFDQFERCCISDDTDRRMLEADRRFFAE 148 Query: 121 YEKSRQAVIALGAEGKTEEALH-AVEHQVVPPGDKLINALAAHRRYNEQLGQQGVQTAQS 179 YEK R A++ LG+ GK E+AL A+E ++ P G +L+ L H+ YNEQLG+QG + A Sbjct: 149 YEKHRMAMVDLGSAGK-EDALRVAMEEKMAPTGQRLMAVLNEHKNYNEQLGRQGAEAADE 207 Query: 180 SLRSAVVQAVLMSLAVCAVVAVVSLLISRTISTALGEAIMTARAIATGDLSTRIRRRAND 239 L+ ++ + + V +V+ +SLL+ R I +L A A I GDLS++I R D Sbjct: 208 HLQLSIYLTIAAATLVIVMVSALSLLLVRNIRHSLHAATELADTIGRGDLSSQIVLRGRD 267 Query: 240 EVGQLMQAIQQMSDSLVRIVSNVRNSANTIATATAEIAAGNLDLSARTEAQAGSIEETAS 299 EV QLM ++Q+M+ SLV+I+ V +SA TI TAT EIA+GNLDLS+RTEAQAGS+EET S Sbjct: 268 EVTQLMLSLQRMTGSLVQILGKVNDSAATIETATREIASGNLDLSSRTEAQAGSLEETVS 327 Query: 300 AMEQLTATVRQNADNAQQANVLATSASSVATEGGQAVQQVVQTMDEINASSLRIVDIISV 359 AM QLT TV+QNADNA+QA+ LA SAS VA + G+ V +V+ TM+ INASSL+IVDIISV Sbjct: 328 AMGQLTTTVKQNADNARQADELAQSASGVAGQAGKVVDEVILTMEAINASSLKIVDIISV 387 Query: 360 IDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRSLAQRSAAAAHEIKELIDDSVGK 419 IDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSA+AA EIKELIDDSV K Sbjct: 388 IDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAREIKELIDDSVNK 447 Query: 420 VESGSRLVAQAGQTMQHVVDSVRRVTDIVADITAASAEQSDGIVQVNQAI 469 V+SGSRLV QAG TM+ VV SVRRV+ IV DI+ AS EQSDGI QVNQAI Sbjct: 448 VQSGSRLVLQAGSTMEEVVTSVRRVSGIVRDISVASGEQSDGIAQVNQAI 497 >gi|300313312|ref|YP_003777404.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 544 Score = 457 bits (1177), Expect = e-130, Method: Compositional matrix adjust. Identities = 253/491 (51%), Positives = 343/491 (69%), Gaps = 1/491 (0%) Query: 1 MTIANKLRLLLLAVVLGLASLTGLSIYHSNRIADAASYSAVNTVPSLLVLGEAGEAVFRI 60 ++I N+L +L+ AV++G L G ++ NR AS+++ N VPSLL L + + + Sbjct: 3 LSIVNRLYILIGAVIIGCVVLAGANMVKMNRTYADASFASENVVPSLLSLSTITQGINGM 62 Query: 61 RVGIWRYAAATDAATRAKLEKSMNTNRDKVIAAFDRYEKEDLSDATDRQLLQADRDAFAD 120 R+ WRY A T++ R +E S N+ + +V A +YE+E L+DATD++LL+A R ++ + Sbjct: 63 RIATWRYLALTESVDRGAIESSFNSAKKRVEDALQKYEQEQLADATDKELLRAVRVSYQE 122 Query: 121 YEKSRQAVIALGAEGKTEEALHAVEHQVVPPGDKLINALAAHRRYNEQLGQQGVQTAQSS 180 ++KS + L G+ EA A+ P D+L A+ R YNE++ Q G AQ + Sbjct: 123 FDKSLAEALKLANAGQWNEA-RALLANNQPVIDRLNKAMTELRAYNEKISQAGAHRAQET 181 Query: 181 LRSAVVQAVLMSLAVCAVVAVVSLLISRTISTALGEAIMTARAIATGDLSTRIRRRANDE 240 + +A + A+L+S AV AVV ++ LLI+R I+ +L EA+ A IA GDL+ I + DE Sbjct: 182 MAAARLHAILISAAVIAVVTLMGLLIARKIARSLAEAVELAETIAEGDLTREIDTSSTDE 241 Query: 241 VGQLMQAIQQMSDSLVRIVSNVRNSANTIATATAEIAAGNLDLSARTEAQAGSIEETASA 300 +G LM A+ +M+ +LV IV+ VR NT+A A+++IA GN+DLSARTE QA S+E+TA+A Sbjct: 242 IGHLMAAMAKMNRNLVTIVATVRTGVNTVAAASSQIATGNMDLSARTEQQASSLEQTAAA 301 Query: 301 MEQLTATVRQNADNAQQANVLATSASSVATEGGQAVQQVVQTMDEINASSLRIVDIISVI 360 E+LT+TV+QNA+NA QA+ LA++AS +A EGG+ V+QVV TM+EI +SSL I DIISVI Sbjct: 302 TEELTSTVKQNAENALQADQLASAASGIAAEGGEVVKQVVTTMEEIRSSSLNIADIISVI 361 Query: 361 DGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRSLAQRSAAAAHEIKELIDDSVGKV 420 DGIAFQTNILALNAAVEAARAGEQGRGFAVVA EVRSLAQRSA+AA EIK+LIDDSV KV Sbjct: 362 DGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSASAAKEIKQLIDDSVSKV 421 Query: 421 ESGSRLVAQAGQTMQHVVDSVRRVTDIVADITAASAEQSDGIVQVNQAIXECGAGGAGGC 480 E+GS+LV AG TMQ VV+SVRRVTD+V +I+AAS EQS GI QVNQAI Sbjct: 422 ENGSQLVMSAGNTMQQVVESVRRVTDVVTEISAASREQSLGIEQVNQAISHMDQSTQENA 481 Query: 481 GLAVDAGAGCR 491 L +A A + Sbjct: 482 ALVEEATAAAQ 492 >gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 375 bits (963), Expect = e-105, Method: Compositional matrix adjust. Identities = 216/386 (55%), Positives = 277/386 (71%), Gaps = 1/386 (0%) Query: 86 NRDKVIAAFDRYEKEDLSDATDRQLLQADRDAFADYEKSRQAVIALGAEGKTEEALHAVE 145 N K A + EK LS A R+L Q+ ++A + Y R + L + K EEA+ + Sbjct: 91 NSAKTTEALGKMEK-ILSTARGRELFQSIQEARSAYNTPRDKLRELIKQQKKEEAVELLF 149 Query: 146 HQVVPPGDKLINALAAHRRYNEQLGQQGVQTAQSSLRSAVVQAVLMSLAVCAVVAVVSLL 205 QV+P DK L + + L +Q V + + +SA+ + +S A+ + + L Sbjct: 150 TQVIPAQDKYFQVLDQFVAFQKSLMEQSVTEGRDTTQSAIAMMLGLSAVAIALCVIAAWL 209 Query: 206 ISRTISTALGEAIMTARAIATGDLSTRIRRRANDEVGQLMQAIQQMSDSLVRIVSNVRNS 265 ++R+I+ L EA+ A A+A GDL+ +I + DE G L+ A++ M+DSL RIVS VR Sbjct: 210 VTRSITRPLNEAVSVASAVAAGDLTVQIGATSKDETGALLAALKSMNDSLHRIVSEVRQG 269 Query: 266 ANTIATATAEIAAGNLDLSARTEAQAGSIEETASAMEQLTATVRQNADNAQQANVLATSA 325 ++TI TA+AEIA GNLDLS+RTE QAG++EETASAME+LT+TV+QNADNA+QAN LA +A Sbjct: 270 SDTINTASAEIATGNLDLSSRTEEQAGALEETASAMEELTSTVKQNADNARQANSLAATA 329 Query: 326 SSVATEGGQAVQQVVQTMDEINASSLRIVDIISVIDGIAFQTNILALNAAVEAARAGEQG 385 S VA +GG V QVVQTM EIN +S +IVDIISVIDGIAFQTNILALNAAVEAARAGEQG Sbjct: 330 SEVAVQGGSVVGQVVQTMGEINDASRKIVDIISVIDGIAFQTNILALNAAVEAARAGEQG 389 Query: 386 RGFAVVAAEVRSLAQRSAAAAHEIKELIDDSVGKVESGSRLVAQAGQTMQHVVDSVRRVT 445 RGFAVVA+EVR+LAQRSA+AA EIK LIDDSV +V++GSRLV QAG TM VV SVRRVT Sbjct: 390 RGFAVVASEVRTLAQRSASAAKEIKALIDDSVSRVDNGSRLVEQAGTTMTEVVASVRRVT 449 Query: 446 DIVADITAASAEQSDGIVQVNQAIXE 471 D+VA+I+AAS EQSDGI Q+N AI + Sbjct: 450 DVVAEISAASNEQSDGIEQINHAITQ 475 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 359 bits (921), Expect = e-100, Method: Compositional matrix adjust. Identities = 223/477 (46%), Positives = 307/477 (64%), Gaps = 11/477 (2%) Query: 1 MTIANKL-----RLLLLAVVLGLASLTGLSIYHSNRIADAASYSAVNTVPSLLVLGEAGE 55 + IA KL +++L V+LGL +I + A++ A N +P++ Sbjct: 7 LKIAKKLIVSFAAVIVLTVILGL-----FAIRELKAVNQASTDMATNWLPTIKAAQSIQA 61 Query: 56 AVFRIRVGIWRYAAATDAATRAKLEKSMNTNRDKVIAAFDRYEKEDLSDATDRQLLQADR 115 ++ R+R+ + A A R K++ + D + YE + +S+A ++ L + Sbjct: 62 SLPRMRISELQMVTAATPAEREDALKAIKSRLDILAKQRAAYEAQ-ISEAEEKVLYEKFS 120 Query: 116 DAFADYEKSRQAVIALGAEGKTEEALHAVEHQVVPPGDKLINALAAHRRYNEQLGQQGVQ 175 +FA Y + + + + AEGK EEA + +I + + N+ ++ Sbjct: 121 KSFATYLEIDKELARISAEGKEEEARALFKGDSNKTFRSMIENMDEIIKVNDAGSKRSDD 180 Query: 176 TAQSSLRSAVVQAVLMSLAVCAVVAVVSLLISRTISTALGEAIMTARAIATGDLSTRIRR 235 A+ RSA + + + A+ + ++++++R +S L EA+ A +A GDL+ IR Sbjct: 181 MAEQVYRSATLWIIGLLAAIVVIALGLAVMVARAVSRPLNEAVAVAERVAQGDLTMDIRA 240 Query: 236 RANDEVGQLMQAIQQMSDSLVRIVSNVRNSANTIATATAEIAAGNLDLSARTEAQAGSIE 295 + DE G LM +++ M+DSL +IV VR +TI TA+ EIA GNLDLS+RTE QAGS+E Sbjct: 241 TSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGNLDLSSRTEEQAGSLE 300 Query: 296 ETASAMEQLTATVRQNADNAQQANVLATSASSVATEGGQAVQQVVQTMDEINASSLRIVD 355 ETASAME+LTATV+QNADNA+QAN LA SAS +AT+GG V QVVQTM+ I SS RI D Sbjct: 301 ETASAMEELTATVKQNADNARQANQLAASASDIATQGGAVVGQVVQTMEGITESSNRIAD 360 Query: 356 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRSLAQRSAAAAHEIKELIDD 415 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSA+AA EIK+LIDD Sbjct: 361 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLIDD 420 Query: 416 SVGKVESGSRLVAQAGQTMQHVVDSVRRVTDIVADITAASAEQSDGIVQVNQAIXEC 472 SV KV +GS+LV +AG TM VV SVRRVTD+VA+I+AAS EQS GI ++N+AI + Sbjct: 421 SVEKVGAGSQLVERAGATMSEVVASVRRVTDVVAEISAASNEQSTGIEEINRAITQM 477 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.126 0.329 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 981,981 Number of extensions: 31684 Number of successful extensions: 539 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 388 Number of HSP's successfully gapped: 70 Length of query: 503 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 414 Effective length of database: 1,198,455 Effective search space: 496160370 Effective search space used: 496160370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)