BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0053.p (213 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313312|ref|YP_003777404.1| methyl-accepting chemotaxis pro... 117 7e-28 gi|300312220|ref|YP_003776312.1| methyl-accepting chemotaxis tra... 111 5e-26 gi|300312219|ref|YP_003776311.1| methyl-accepting chemotaxis tra... 96 3e-21 gi|300310428|ref|YP_003774520.1| methyl-accepting chemotaxis tra... 91 7e-20 >gi|300313312|ref|YP_003777404.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 544 Score = 117 bits (293), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 69/212 (32%), Positives = 119/212 (56%), Gaps = 4/212 (1%) Query: 1 MTVAKKLYALAAIAILGTLLIVGVQTYHLHRVDSAASYASANTVPSILELDSVIDGAYAM 60 +++ +LY L I+G +++ G ++R + AS+AS N VPS+L L ++ G M Sbjct: 3 LSIVNRLYILIGAVIIGCVVLAGANMVKMNRTYADASFASENVVPSLLSLSTITQGINGM 62 Query: 61 RISIWKYLANPTPEQRQETEKVINASSSQIARALDTYEKEDISDATDAQMLKDIRQHIAT 120 RI+ W+YLA R E N++ ++ AL YE+E ++DATD ++L+ +R Sbjct: 63 RIATWRYLALTESVDRGAIESSFNSAKKRVEDALQKYEQEQLADATDKELLRAVRVSYQE 122 Query: 121 YDASLAKVMILAQKDA-VAARNAMAADQAIVDTMMSALDKQKKYNQKLGKAAAQTATATK 179 +D SLA+ + LA AR +A +Q ++D + A+ + + YN+K+ +A A A T Sbjct: 123 FDKSLAEALKLANAGQWNEARALLANNQPVIDRLNKAMTELRAYNEKISQAGAHRAQETM 182 Query: 180 NQAMVTALAISALIAALV---GMILYFLLARS 208 A + A+ ISA + A+V G+++ +ARS Sbjct: 183 AAARLHAILISAAVIAVVTLMGLLIARKIARS 214 >gi|300312220|ref|YP_003776312.1| methyl-accepting chemotaxis transducer transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 535 Score = 111 bits (277), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 73/204 (35%), Positives = 111/204 (54%), Gaps = 10/204 (4%) Query: 1 MTVAKKLYALAAIAILGTLLIVGVQTYHLHRVDSAASYASANTVPSILELDSVIDGAYAM 60 MT+AKKL+ L ILG + + Y V AASY+S NTVPS+L L + A+ + Sbjct: 1 MTIAKKLHLLTLAVILGLASLSLLGIYQSREVAKAASYSSVNTVPSLLALGDAGEAAFGI 60 Query: 61 RISIWKYLANPTPEQRQETEKVINASSSQIARALDTYEKEDISDATDAQMLKDIRQHIAT 120 R+ IWKY A P R EK + ++ A D Y +EDISD TD ++L+ RQ A Sbjct: 61 RVGIWKYAAATDPVSRTRLEKAMAEQHDRVLAAFDRYAREDISDDTDRKLLEADRQAFAD 120 Query: 121 YDASLAKVMILAQKDAVAARNA---MAADQAIV---DTMMSALDKQKKYNQKLGKAAAQT 174 Y+ A V+ + + +RN A +Q +V D +++AL + +KY ++LG+ +T Sbjct: 121 YEKQRAVVLRIGAE----SRNEEALRAIEQLVVPPGDKLIAALAEHRKYKEQLGRQGVET 176 Query: 175 ATATKNQAMVTALAISALIAALVG 198 A + A+ +S L+AA+VG Sbjct: 177 AEGILQSSTYQAILVSLLVAAIVG 200 >gi|300312219|ref|YP_003776311.1| methyl-accepting chemotaxis transducer transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 563 Score = 95.5 bits (236), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 67/214 (31%), Positives = 113/214 (52%), Gaps = 5/214 (2%) Query: 1 MTVAKKLYALAAIAILGTLLIVGVQTYHLHRVDSAASYASANTVPSILELDSVIDGAYAM 60 MT+A+KLY L +LG ++ V Y HR+ S+A Y++ +T+PS+L L+ D Y + Sbjct: 31 MTIARKLYLLIVAVVLGVIMQAVVGLYQSHRIASSAGYSATDTIPSVLALNGATDAIYGI 90 Query: 61 RISIWKYLANPTPEQRQETEKVINASSSQIARALDTYEKEDISDATDAQMLKDIRQHIAT 120 R++I +Y+ PT R++T + A + D +E+ ISD TD +ML+ R+ A Sbjct: 91 RLNIARYVIEPTA--REKTVANMAALHQKAQDFFDQFERCCISDDTDRRMLEADRRFFAE 148 Query: 121 YDA-SLAKVMILAQKDAVAARNAMAADQAIVDT-MMSALDKQKKYNQKLGKAAAQTATAT 178 Y+ +A V + + A R AM A +M+ L++ K YN++LG+ A+ A Sbjct: 149 YEKHRMAMVDLGSAGKEDALRVAMEEKMAPTGQRLMAVLNEHKNYNEQLGRQGAEAADEH 208 Query: 179 KNQAMVTALAISALIAALVGMILYFLLARSAHPS 212 ++ +A + L+ +V L LL R+ S Sbjct: 209 LQLSIYLTIAAATLVIVMVSA-LSLLLVRNIRHS 241 >gi|300310428|ref|YP_003774520.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 517 Score = 90.9 bits (224), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 63/190 (33%), Positives = 101/190 (53%), Gaps = 9/190 (4%) Query: 24 VQTYHLHRVDSAASYASANTVPSILELDSVIDGAYAMRISIWKYLANPTPEQRQETEKVI 83 V + V +AA+YA NTVPS+ LD A++ I++YL NP P R K + Sbjct: 32 VSLRQIENVYTAANYAQVNTVPSVTTLDDAQAAFNAIQGRIYQYLVNPDPRDRSRLIKEM 91 Query: 84 NASSSQIARALDTYEKEDISDATDAQMLKDIRQHIATYDASLAKVMILAQKDAVAARNAM 143 + +++ R L TY I+D TD ML D R HIA ++ + +++ +A++D V + Sbjct: 92 DVQRARMQRHLHTYANAYITDETDGAMLADDRAHIAAFEEVVRRLLAVAEEDRVEDSLGL 151 Query: 144 AADQ--AIVDTMMSALDKQKKYNQKLGKAAAQTATATKNQAMVTALAISALIAALVGMIL 201 + + A +DT+ ++ + + YN LG A+A TA QA+ T AL+A V +IL Sbjct: 152 SVLEFRAAIDTVQASFARHRSYNVALGDASALTAREIIGQALETV----ALVAGGVLLIL 207 Query: 202 Y---FLLARS 208 +LL R+ Sbjct: 208 LGAGWLLTRT 217 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.125 0.327 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 367,237 Number of extensions: 10165 Number of successful extensions: 65 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 63 Number of HSP's successfully gapped: 4 Length of query: 213 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 132 Effective length of database: 1,236,335 Effective search space: 163196220 Effective search space used: 163196220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)