BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0052.p (747 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase ... 1402 0.0 gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydroge... 191 2e-49 gi|300313158|ref|YP_003777250.1| anaerobic dehydrogenase family ... 78 3e-15 gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase... 70 7e-13 gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-conta... 66 1e-11 >gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase large subunit [Herbaspirillum seropedicae SmR1] Length = 922 Score = 1402 bits (3628), Expect = 0.0, Method: Compositional matrix adjust. Identities = 678/763 (88%), Positives = 711/763 (93%), Gaps = 19/763 (2%) Query: 1 VPVCYEDLELADLIVLVGSNAAWCHPILFQRIAKLKETRPALRLIVIDPRRTATCELADL 60 VPVCYEDLELADL+VLVGSNAAWCHPILFQRIA+LKETRPALRL+VIDPRRTATCELADL Sbjct: 163 VPVCYEDLELADLVVLVGSNAAWCHPILFQRIARLKETRPALRLVVIDPRRTATCELADL 222 Query: 61 HLPVKAGTDVWLFNGLMSYLAHRGLRDNAFMAAHTNGFDEALAVADAECADPATVARICK 120 HLP+K GTDVWLFNGLMSYLAHRGLRDNAF+ AHT GFDEALAVADA+CADPATVAR+CK Sbjct: 223 HLPIKPGTDVWLFNGLMSYLAHRGLRDNAFIEAHTKGFDEALAVADADCADPATVARLCK 282 Query: 121 VDLQDLLDFYEAFAETEKVVTGFSMGVNQSSVGTDKVNSIINCHLATGRIGKPGMGPFSI 180 +DL+DLL FYEAFA TEKVVTGFSMGVNQSS GTDKVNSIINCHLATGRIGKPGMGPFSI Sbjct: 283 IDLKDLLAFYEAFANTEKVVTGFSMGVNQSSAGTDKVNSIINCHLATGRIGKPGMGPFSI 342 Query: 181 TGQPNAMGGREVGGLANMLAAHMDLDQPLHREIVQEFWQSPRIADKPGLKAVDMFRAIEE 240 TGQPNAMGGREVGGLANMLAAHMDLDQPLHR+IVQ+FWQSPRIADKPGLKAVDMFRAIEE Sbjct: 343 TGQPNAMGGREVGGLANMLAAHMDLDQPLHRQIVQDFWQSPRIADKPGLKAVDMFRAIEE 402 Query: 241 GRIKAVWIMATNPLVSLPDADLVQRALQKCELVVSSDVITHTDTNALAHVLLPALAWGEK 300 GRIKAVWIMATNP+VSLPDADLVQRALQKCELVVSSDVIT TDTNALAHVLLPALAWGEK Sbjct: 403 GRIKAVWIMATNPMVSLPDADLVQRALQKCELVVSSDVITQTDTNALAHVLLPALAWGEK 462 Query: 301 DGTVTNSERRISRQRAFLTAPGEARGDWQIICEVAQRMGFAGFDFAGPHAIFDEHARLSA 360 DGTVTNSERRISRQRAFL+APGEARGDW+IIC+VA+RMGF GFDFAGPH IFDEHARLSA Sbjct: 463 DGTVTNSERRISRQRAFLSAPGEARGDWRIICDVARRMGFDGFDFAGPHEIFDEHARLSA 522 Query: 361 HRNGEADAGGEHRARRVFNLSGLTGMDKAAYDAMAPIQWPVQRLSDGSVSGTARVLEDHR 420 HRNGEADA R RRVFNL+GLTGMD+AAYDA+APIQWPVQRLSDGSVSGTARVLEDHR Sbjct: 523 HRNGEADAS---RVRRVFNLAGLTGMDRAAYDALAPIQWPVQRLSDGSVSGTARVLEDHR 579 Query: 421 FTHADGRARFVPTAPRTPGHAVCEDYPLILNTGRVRDHWHTMTRTGKSATLASHIPESFI 480 F+HADGRARFVPTAPR+ HA CEDYPLILNTGRVRDHWHTMTRTG+SATLASHIPESFI Sbjct: 580 FSHADGRARFVPTAPRSAAHAACEDYPLILNTGRVRDHWHTMTRTGRSATLASHIPESFI 639 Query: 481 DLHPQDALLYGVREGGLARISSRWGAMVARVQHGA----------------SHXYARVGA 524 D+HPQDALLYGVREGGLARISSRWGAMVARVQHG S ARVGA Sbjct: 640 DIHPQDALLYGVREGGLARISSRWGAMVARVQHGGGIARGSVFVPIHWNGQSASDARVGA 699 Query: 525 VVNPVVDPVSGEPEFKHTPVAIEEFRVGWHGFILTRHALDLDDITHWTRVQGRDFVRYEL 584 VVNPVVDPVSGEPEFKHTPVAIEEFRV WHGFILTRHALDLD +THWTRVQGRDF+RYEL Sbjct: 700 VVNPVVDPVSGEPEFKHTPVAIEEFRVSWHGFILTRHALDLDQVTHWTRVQGRDFMRYEL 759 Query: 585 AGRNHISDHTAWARALLGVQADDADWLEYEDLSDGSYRAVHVVDDRIDMCLFMSARPDLP 644 AGRNHI DH+AWAR+LLGVQ ADWLEYEDLSDGSYRAVHVV+DRIDMCLFMSARPDLP Sbjct: 760 AGRNHIPDHSAWARSLLGVQDPQADWLEYEDLSDGSYRAVHVVEDRIDMCLFMSARPDLP 819 Query: 645 SRSWLAGLFAKEKLEEADRVGLLVGQPIEKGADAGPTVCSCFGVGRNTICNAIRAQGLTE 704 SRSWLAGLFAKEKLEEADRVGLLVGQPIEKGAD GPTVCSCFGVGRNTICNAIR QGL+E Sbjct: 820 SRSWLAGLFAKEKLEEADRVGLLVGQPIEKGADTGPTVCSCFGVGRNTICNAIRTQGLSE 879 Query: 705 VSQVTACVKAGGNCGSCVPEIKKLMVEIRVQGEAEARERETAA 747 V QVTAC+KAGGNCGSCVPEIKKL+VE+R+Q AE RE AA Sbjct: 880 VGQVTACLKAGGNCGSCVPEIKKLIVEVRLQDAAELREGAKAA 922 >gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydrogenase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 957 Score = 191 bits (485), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 151/559 (27%), Positives = 252/559 (45%), Gaps = 51/559 (9%) Query: 5 YEDLELADLIVLVGSNAAWCHPILFQRIAKLKETRPALRLIVIDPRRTATCE----LADL 60 +E + +D+I+++G+N A HP+ +I + R +LIVIDPR T + AD Sbjct: 397 FESVMKSDVILIMGANPAAGHPVFASQIKR--RVRQGAKLIVIDPRTTEMVKSPHIQADY 454 Query: 61 HLPVKAGTDVWLFNGLMSYLAHRGLRDNAFMAAHTN--GFDEALAVADAECADPATVARI 118 HL ++ GT+ + L + GL+D A++ + + + E P +A I Sbjct: 455 HLKLRPGTNTAIITALAHVILSEGLQDEAYIQERCDLQSYQDWKEFVLREDNSPEAMAAI 514 Query: 119 CKVDLQDLLDFYEAFAETEKVVTGFSMGVNQSSVGTDKVNSIINCHLATGRIGKPGMGPF 178 V + + +A + +GV + + G+ V I N + TG +G+ G+G Sbjct: 515 TGVPAEIIRGAARLYATGGNAAIYYGLGVTEHAQGSTTVMGIANLAMCTGNVGREGVGVN 574 Query: 179 SITGQPNAMGGREVGGLANMLAAHMDLDQPLHREIVQEFWQSPRIADKPGLKAVDMFRAI 238 + GQ N G ++G + L + + R ++ W + +PGL+ +MF A Sbjct: 575 PLRGQNNVQGSCDMGSFPHELPGYRHISDSTVRNQFEQAW-GVTLNPEPGLRIPNMFDAA 633 Query: 239 EEGRIKAVWIMATNPLVSLPDADLVQRALQKCELVVSSDVITHTDTNALAHVLLPALAWG 298 +G K ++ + + S P+ V ALQ E +V D+ + +T AHV LP ++ Sbjct: 634 LDGSFKGLYCEGEDIVQSDPNTQHVAAALQAMECIVVQDLFLN-ETAKYAHVFLPGSSFL 692 Query: 299 EKDGTVTNSERRISRQRAFLTAPGEARGDWQIICEVAQRMGFAGFDFAGPHAIFDEHARL 358 EKDGT TN+ERRISR R + P + DW++ +A+ +G+ + P I DE A L Sbjct: 693 EKDGTFTNAERRISRVRKVM-PPKAGKSDWEVTVALAEALGYP-MPYNHPSEIMDEIAAL 750 Query: 359 SAHRNGEADAGGEHRARRVFNLSGLTGMDKAAYDAMAPIQWPVQRLSDGSVSGTARVLED 418 + +G DK + + IQWP ++ + GT + Sbjct: 751 TPSFHG-------------------VSYDK--LEKLGSIQWPC---NESAPEGTPIM--- 783 Query: 419 HRFTHADGRARFVPTAPRTPGHAVCEDYPLILNTGRVRDHWHTMTRTGKSATLASHIPES 478 H + G+ +F+ T V + YPLIL TGR+ ++ +T ++ H E Sbjct: 784 HVGSFIRGKGKFINTQYFATDEKVTQRYPLILTTGRILSQYNVGAQTRRTENSQWH-SED 842 Query: 479 FIDLHPQDALLYGVREGGLARISSRWGAMVAR------VQHGASHX---YARVGA--VVN 527 +++HP DA G+REG + SR G V R VQ G + + GA + Sbjct: 843 RLEIHPHDAEDRGIREGDWVGVESRAGQTVLRATVTEKVQPGVVYTTFHFPESGANVITT 902 Query: 528 PVVDPVSGEPEFKHTPVAI 546 D + PE+K T V + Sbjct: 903 DNSDWATNCPEYKVTAVQV 921 >gi|300313158|ref|YP_003777250.1| anaerobic dehydrogenase family protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 77.8 bits (190), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 122/530 (23%), Positives = 208/530 (39%), Gaps = 48/530 (9%) Query: 6 EDLELADLIVLVGSNAAWCHPILFQRIAKLKETRPALRLIVIDPRRTATCELADLHLPVK 65 E ++ A LI++ G N + + R+ K R RLI IDP R+ T E H+ + Sbjct: 160 EQVDHAKLILIWGGNPIASNLHFWTRVQTAK--RNGARLIAIDPYRSLTAEKCHQHIALL 217 Query: 66 AGTDVWLFNGLMSYLAHRGLRDNAFMAAHTNGFDEALAVADAECADPATVARICKVDLQD 125 GTD L G+M L L D+ ++A HT GF E L AE PA A +C + ++ Sbjct: 218 PGTDAALALGMMHVLISEDLLDHDYIARHTLGF-EQLRERVAEWT-PARTAEVCGISAEE 275 Query: 126 LLDFYEAFAETEKVVTGFSMGVNQSSVGTDKVNSIINCHLATGRIGKPGMGPFSITGQPN 185 +L+ + G I + R+ GM ++ P Sbjct: 276 VLNLAREYGRVALAGEGV----------------FIRANYGLQRVRGGGMAARNVACLPA 319 Query: 186 AMGG--REVGGLANMLAAHMDLD-QPLHR-EIVQEFWQSPRIADKPGLKAVDMFRAIEE- 240 +G GG+ ++ D L R ++ + R+ + + + A + Sbjct: 320 LVGAWRHPAGGVQMSVSDSFPKDAAALSRPDLAAGHGKPARVINMSTIGDALLHPASADF 379 Query: 241 -GRIKAVWIMATNPLVSLPDADLVQRALQKCELVVSSDVITHTDTNALAHVLLPALAWGE 299 +++A+ + +NP+ P++ V + + +L TDT A +LLPA E Sbjct: 380 GPKVEALIVYNSNPVAVAPESAKVAQGFAREDLFTVVLEHFQTDTADYADILLPATTQLE 439 Query: 300 K-DGTVTNSERRISRQRAFLTAPGEARGDWQIICEVAQRMGFAGFDFAGPHAIFDEHARL 358 D + I A + GEA+ + +I +A MGF F D+ A + Sbjct: 440 HIDVHASYGHVYIMANNAAIAPMGEAKPNTEIFRRLAAAMGFTEDCFQDSD---DQLAAV 496 Query: 359 SAHRNGEADAGGEHRARRVFNLSGLTGMDKAAYDAMAPIQWPVQRLSDGSVSGTARVL-E 417 + R+ AG + + + + +A P + SG E Sbjct: 497 AFKRDDPRSAGYDWEQ-----------LKQKGWQRLAVPVAPFAEGGFATPSGKCEFYSE 545 Query: 418 DHRFTHADGRARFVPTAPRTPGHA-VCEDYPLILNTGRVRDHWHTMTRTGKSATLASHIP 476 + D ++P + + YPL + + R+ ++ +S L Sbjct: 546 QMKAAGLDPLPAYIPPYESVASNPQLAARYPLAMISPPARNFLNSSFVNVQS--LRDTEG 603 Query: 477 ESFIDLHPQDALLYGVREGGLARISSRWGAMVARVQHGASHXYARVGAVV 526 E +D+HPQDA G+ +G + RI + G++ ARV+ ARVG VV Sbjct: 604 EPHLDMHPQDAAARGISQGDMVRIFNDRGSLQARVR---VTDKARVGLVV 650 >gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 790 Score = 70.1 bits (170), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 81/333 (24%), Positives = 132/333 (39%), Gaps = 51/333 (15%) Query: 3 VCYEDLELADLIVLVGSNAAWCHPILFQRIAKLKETRPALRLIVIDPRR----------- 51 V +D D I+ G N P + + ++++ + +I +P R Sbjct: 224 VTLDDFGQTDCILFFGHNTGTNAPRMLHPLEEVRKR--GVPVITFNPLRERGLERFVNPQ 281 Query: 52 -------TATCELADLHLPVKAGTDVWLFNGLMSYLAHRG----------LRDNAFMAAH 94 A +++ +L +K G D G+ + R L D AF+ H Sbjct: 282 SPKEMVTPAHTDISTQYLQIKIGGDSAAAIGMAKRILERDDKARQEGLPRLLDLAFIEEH 341 Query: 95 TNGFDEALAVADAECADP-ATVARICKVDLQDLLDFYEAFAETEKVVTGFSMGVNQSSVG 153 T+GF++ ADA A P + R V +L + +A + + + MGV Q Sbjct: 342 TSGFEQ---FADAVRAAPWDELERHAGVSRAELELAADTYAGAGRAMLMYGMGVTQHREA 398 Query: 154 TDKVNSIINCHLATGRIGKPGMGPFSITGQPNAMGGREVGGLAN-MLAAHMDLDQPLHRE 212 ++ + N L G IGKPG G I G N G R VG L + L H E Sbjct: 399 VRTIHMLTNLLLMRGNIGKPGAGICPIRGHSNVQGQRTVGITEKPELVPNDKLRALYHFE 458 Query: 213 IVQEFWQSPRIADKPGLKAVDMFRAIEEGRIKAVWIMATNPLVSLPDADLVQRALQKCEL 272 E GL V+ I +G + A +++ N + ++PD +++ A +K L Sbjct: 459 PPME----------KGLNTVEACEKIRDGELSAFFMLGGNFVRAIPDHGVMEAAWRKLPL 508 Query: 273 VVSSDVITHTDTNALAH----VLLPALAWGEKD 301 V V+TH + + + H +LP L E D Sbjct: 509 TVQ--VVTHFNRSCVIHGQTSYVLPCLGRIEID 539 >gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-containing) formate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 775 Score = 65.9 bits (159), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 75/333 (22%), Positives = 129/333 (38%), Gaps = 51/333 (15%) Query: 3 VCYEDLELADLIVLVGSNAAWCHPILFQRIAKLKETRPALRLIVIDPRR----------- 51 V ED + D ++L G N A HP + + ++ ++ I+P + Sbjct: 204 VLLEDFDHCDTLLLFGQNPATNHPRMMGELR--HASKRGATIVAINPLKERGLERFADPQ 261 Query: 52 -------TATCELADLHLPVKAGTDVWLFNGLMSYL-----------AHRGLRDNAFMAA 93 + ++ + + G D+ L G++ A R + D F+ Sbjct: 262 SKVEMLTMGSTRISSMFIHPTLGGDLALIKGVIKRTIELDDAARAAGAERVI-DVKFIEE 320 Query: 94 HTNGFDEALAVADAECADPATVARICKVDLQDLLDFYEAFAETEKVVTGFSMGVNQSSVG 153 HT GF+E A A AE D + V +D+ + + + + V+ + MG+ Q Sbjct: 321 HTAGFEEFAAQARAESWD--DIVAESGVSREDIEKLAQVYVKGKAVIATWGMGLTQHKHA 378 Query: 154 TDKVNSIINCHLATGRIGKPGMGPFSITGQPNAMGGREVGGLANMLAAHMDLDQPLHREI 213 + + N + G IG+ G G + G N G R VG A +D + Sbjct: 379 VATIQLLSNMMMLRGNIGRQGAGLCPVRGHSNVQGDRTVGIDEKPTPAFLD-------RL 431 Query: 214 VQEF-WQSPRIADKPGLKAVDMFRAIEEGRIKAVWIMATNPLVSLPDADLVQRALQKCEL 272 Q F +++PR G V A+ EGR K + N ++ PD A++ C L Sbjct: 432 EQVFGFKAPR---HHGNDTVGSVMAMLEGRTKVFIGLGGNFAMATPDTPRTFEAMRSCNL 488 Query: 273 VVSSDVITHTDTNALAH----VLLPALAWGEKD 301 V + T + + L H ++LP + E D Sbjct: 489 TVH--ITTKLNRSHLVHGKDALILPTMGRTEID 519 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,512,481 Number of extensions: 113564 Number of successful extensions: 300 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 290 Number of HSP's successfully gapped: 5 Length of query: 747 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 655 Effective length of database: 1,184,250 Effective search space: 775683750 Effective search space used: 775683750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)