BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0050.p (407 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312207|ref|YP_003776299.1| FAD-dependent pyridine nucleoti... 799 0.0 gi|300312205|ref|YP_003776297.1| nitrite reductase large subunit... 182 4e-47 gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspi... 55 9e-09 >gi|300312207|ref|YP_003776299.1| FAD-dependent pyridine nucleotide-disulfide oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 413 Score = 799 bits (2063), Expect = 0.0, Method: Compositional matrix adjust. Identities = 387/406 (95%), Positives = 402/406 (99%) Query: 2 TTAKPSLVVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRIMLSPVLAGDKSID 61 +TA+PSLVVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRIMLSPVLAGDKSID Sbjct: 8 STARPSLVVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRIMLSPVLAGDKSID 67 Query: 62 DIMLNPRAWYDEHGITLHAGDPVVQIDRRQRTVHAQSGLAVKYDRLLLATGSTPFIIPVP 121 DIMLNPRAWYD++GITLHAGDPVV IDRRQRTVHA+SGL V+YDRLLLATGSTPFIIPVP Sbjct: 68 DIMLNPRAWYDDNGITLHAGDPVVHIDRRQRTVHAKSGLTVQYDRLLLATGSTPFIIPVP 127 Query: 122 GHQLPGVIAFRDIQDVDTMLQAARTHRHAVVIGGGLLGLEAANGLMRQGMDVTVVHVTDA 181 GHQLPGVIAFRDIQDVD MLQAARTHRHAVVIGGGLLGLEAANGLMRQGMDVTVVHVTDA Sbjct: 128 GHQLPGVIAFRDIQDVDAMLQAARTHRHAVVIGGGLLGLEAANGLMRQGMDVTVVHVTDA 187 Query: 182 LMNQQLDKPAAALLKKALEDKGLRFLLNAHTDEIVGPDRVTAVRFKDGSSIPADLVVMTA 241 LMNQQLDKPAAALLKKALEDKGLRFLLNAHT+EIVGPDRVTAVRFKDGSSIPADLVVMTA Sbjct: 188 LMNQQLDKPAAALLKKALEDKGLRFLLNAHTEEIVGPDRVTAVRFKDGSSIPADLVVMTA 247 Query: 242 GVRPNIALAKSAGLHCERAIIVDDTLQTYDPRIYAVGECVQHRKATFGLVAPIWDQARVC 301 GVRPNIALAK+AGLHCERAIIVDDTLQTYDPR+YAVGECVQHRKATFGLVAPIWDQARVC Sbjct: 248 GVRPNIALAKAAGLHCERAIIVDDTLQTYDPRVYAVGECVQHRKATFGLVAPIWDQARVC 307 Query: 302 GAHLAGAGHRRYVQQATATKLKVTGVDLYSAGDFIGGEDTEDLVVRDPRRGIYKRLVLRG 361 GAHLAGAGHRRYVQQATATKLKVTGVDLYSAGDFIGG+DTEDL+VRDPRRG+YKRLVLRG Sbjct: 308 GAHLAGAGHRRYVQQATATKLKVTGVDLYSAGDFIGGDDTEDLIVRDPRRGVYKRLVLRG 367 Query: 362 SRLIGAVLYGDVKDGPWYFDLIQSGRDISAFRRELPFGQAMCQHAA 407 +RL+GAVLYGDVKDGPWYFDLIQSGRDIS+FRRELPFGQAMCQ AA Sbjct: 368 TRLVGAVLYGDVKDGPWYFDLIQSGRDISSFRRELPFGQAMCQQAA 413 >gi|300312205|ref|YP_003776297.1| nitrite reductase large subunit [Herbaspirillum seropedicae SmR1] Length = 857 Score = 182 bits (463), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 138/397 (34%), Positives = 210/397 (52%), Gaps = 26/397 (6%) Query: 8 LVVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRIMLSPVLAGDKSIDDIMLNP 67 ++V+G+GM G + +E L + +T+ EP Y+R+ LS AG K+ +D+ L Sbjct: 3 IIVIGHGMVGHKFLECLAESGAGDLQVTILCEEPRPAYDRVHLSEFFAG-KTAEDLSLVA 61 Query: 68 RAWYD------EHGITLHAGDPVVQIDRRQRTVH-AQSGLAVKYDRLLLATGSTPFIIPV 120 ++D + + L+A + ++R+Q V+ A + YD+L+LATGS PF+ V Sbjct: 62 PGFFDRGRSAVQFDLKLNAKAQQIDLERKQVLVNVAGVEETMSYDKLVLATGSYPFVPTV 121 Query: 121 PGHQLPGVIAFRDIQDVDTMLQAARTHRHAVVIGGGLLGLEAANGLMRQGMDVTVVHVTD 180 G+Q +R I+D++ ML+ + VVIGGGLLGLE A L ++ VV Sbjct: 122 AGNQRKDCFVYRTIEDLEAMLECGSRSKIGVVIGGGLLGLECAKALRDLKLETHVVEFAP 181 Query: 181 ALMNQQLDKPAAALLKKALEDKGLRFLLNAHTDEIV-GPDRVTAVRFKDGSSIPADLVVM 239 LM Q+D+ +L++ +E+ G+ +T EIV G + F+DGS + D++V Sbjct: 182 RLMAVQVDESGGRVLRQKIEELGVTAHTQKNTVEIVDGKHHTHRMNFEDGSHLETDMIVF 241 Query: 240 TAGVRPNIALAKSAGLHC--ERAIIVDDTLQTYDPRIYAVGECVQHRKATFGLVAPIWDQ 297 +AG+RP LA+ AGL I +D++ T DP +YA+GEC FGLVAP +D Sbjct: 242 SAGIRPRDELARQAGLKVGDRGGIAIDNSCLTSDPAVYAIGECALWNGKIFGLVAPGYDM 301 Query: 298 ARVCGAHLAGAGHRRYVQQATA-------TKLKVTGVDLYSAGDFIGGE-DTEDLVVRDP 349 AR HL HR + A TKLK+ GVD+ S GD G E + D Sbjct: 302 ARTAAKHL--LSHRDEAIEVPAFNGADMSTKLKLMGVDVASIGDAHGKEAGSRSYQFTDE 359 Query: 350 RRGIYKRLVL--RGSRLIGAVLYGDVKDGPWYFDLIQ 384 R+ IYK++V+ G L+GAV+ GD + Y L+Q Sbjct: 360 RKQIYKKIVVSESGKHLLGAVMVGDASE---YGTLLQ 393 >gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 55.5 bits (132), Expect = 9e-09, Method: Compositional matrix adjust. Identities = 64/233 (27%), Positives = 101/233 (43%), Gaps = 27/233 (11%) Query: 60 IDDIMLNPRAWYDEHGITLHAGDPVVQIDRRQRTVHAQSGLAVKYDRLLLATGSTPFIIP 119 ++DI L D ++LH G V+ D V Q A +L+ATG P Sbjct: 96 LNDIYLR---LLDNAKVSLHRGFAKVE-DAHTVNVDGQRFTA---RHILVATGGRPDKPA 148 Query: 120 VPGHQLPGVIAFRDIQDVDTMLQAARTHRHAVVIGGGLLGLEAANGLMRQGMDVTVVHVT 179 +PG +L G+ + D + AVV+GGG + +E A+ L G +VT+V+ Sbjct: 149 IPGAEL-GITS-------DDFFHLKALPQRAVVLGGGYIAVELASILNGLGCEVTLVYRR 200 Query: 180 DALMNQQLDKPAAALLKKALEDKGLRFLLNAHTDEI-------VGPDRVTAVRFKDGSSI 232 + L+ + +D L + KG+R + NA + I V VR +G ++ Sbjct: 201 ERLL-RNMDADLGIHLADEMAKKGIRIVFNASIEAIEAEQAGEASEASVKTVRLTNGEAL 259 Query: 233 PADLVVMTAGVRPNIA--LAKSAG--LHCERAIIVDDTLQTYDPRIYAVGECV 281 A V+ G N A + AG L AI V+ ++ P I AVG+ + Sbjct: 260 SAQCVLFATGRTANTAGLGLQEAGVKLKANGAIEVNQDFESSVPSILAVGDVI 312 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,283,955 Number of extensions: 54227 Number of successful extensions: 174 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 170 Number of HSP's successfully gapped: 3 Length of query: 407 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 320 Effective length of database: 1,207,925 Effective search space: 386536000 Effective search space used: 386536000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)