BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0028.p (308 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312196|ref|YP_003776288.1| LysR family transcription regul... 587 e-169 gi|300309921|ref|YP_003774013.1| LysR family transcription regul... 282 2e-77 gi|300311458|ref|YP_003775550.1| LysR family transcription regul... 205 4e-54 gi|300312334|ref|YP_003776426.1| transcription regulator protein... 196 3e-51 gi|300312244|ref|YP_003776336.1| LysR family transcription regul... 191 6e-50 >gi|300312196|ref|YP_003776288.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 587 bits (1512), Expect = e-169, Method: Compositional matrix adjust. Identities = 278/302 (92%), Positives = 292/302 (96%) Query: 1 MDKLQAMETFVAVVESGSFVAAVEVTGQSKPAVSRQVSELEQFLGIRLLHRTTRRLSLTD 60 MDKLQAM+TFVAVVESGSFVAAV+VTGQSKPAVSRQVSELEQFLGIRLLHRTTRRLSLTD Sbjct: 1 MDKLQAMQTFVAVVESGSFVAAVDVTGQSKPAVSRQVSELEQFLGIRLLHRTTRRLSLTD 60 Query: 61 EGRVYFTRCKELLESIQDVESEVGLNSDQACGRLRIGAPQDFGVEQLAPLWGKFTEQNPQ 120 EGRVYF RCKELLE+IQDVESEVGLNSDQACGRLRIGAPQDFGVEQLAPLWGKFTEQ+P Sbjct: 61 EGRVYFARCKELLETIQDVESEVGLNSDQACGRLRIGAPQDFGVEQLAPLWGKFTEQHPL 120 Query: 121 ITLNITLSDRTIDLLEEGFDMAIRISTLGDSRMVARPLAPVRMIACASPAFLQRHGTPVS 180 ITLNITLSDRTIDLLEEGFDMAIRIS LGDSRMVARPLAPVRMIACA+P FL RHGTP + Sbjct: 121 ITLNITLSDRTIDLLEEGFDMAIRISALGDSRMVARPLAPVRMIACAAPEFLARHGTPGT 180 Query: 181 PAELRDYPFISYSYRNGGDEWHFKHEHGDTCTVRVRSTVYVNNGATCRAMAVAGQGIAIQ 240 PAELRDYPFISYSYR GGD+W F+H HGDTC VRVRSTVYVNNGATCRAMA+AGQGIAIQ Sbjct: 181 PAELRDYPFISYSYRAGGDDWSFRHVHGDTCQVRVRSTVYVNNGATCRAMAIAGQGIAIQ 240 Query: 241 PDFIVNAAIQSGELVELFEGWSTGEQLVVHTLYPTRTHLPPKVQRMRDFLVEQFKVPPWC 300 PDFIVNAAIQ+G+LVELFEGWSTGEQLVVHTLYPTRTHLPPKVQRMRDFL+EQF+VPPWC Sbjct: 241 PDFIVNAAIQAGQLVELFEGWSTGEQLVVHTLYPTRTHLPPKVQRMRDFLIEQFRVPPWC 300 Query: 301 PK 302 P+ Sbjct: 301 PR 302 >gi|300309921|ref|YP_003774013.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 299 Score = 282 bits (722), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 142/299 (47%), Positives = 194/299 (64%), Gaps = 2/299 (0%) Query: 1 MDKLQAMETFVAVVESGSFVAAVEVTGQSKPAVSRQVSELEQFLGIRLLHRTTRRLSLTD 60 M+KL+ +E F+AV + GSFV A E G SK AVSR V ELE LG RL+ RTTRRLSLT+ Sbjct: 1 MNKLREIECFIAVADLGSFVKAAEALGISKAAVSRTVLELEARLGSRLMQRTTRRLSLTE 60 Query: 61 EGRVYFTRCKELLESIQDVESEVGLNSDQACGRLRIGAPQDFGVEQLAPLWGKFTEQNPQ 120 G +Y RCK+++ ++++ + + S + G LRI AP FGV LAPLW F E++P+ Sbjct: 61 AGALYLERCKQIVAALEEADDLLSSGSAKPTGLLRINAPLTFGVLHLAPLWPMFLERHPE 120 Query: 121 ITLNITLSDRTIDLLEEGFDMAIRISTLGDSRMVARPLAPVRMIACASPAFLQRHGTPVS 180 I+L+ITLSDR +D+++EG+DMAIRI+ L DS +V R L + CASPA+L HGTP Sbjct: 121 ISLDITLSDRIVDIIDEGYDMAIRIARLSDSTLVHRKLTSTSLHVCASPAYLDAHGTPAH 180 Query: 181 PAELRDYPFISYSYRNGGDEWHFKHEHGDTCTVRVRSTVYVNNGATCRAMAVAGQGIAIQ 240 P EL + ISYSY DEW F G +VRV S ++VNNG +C A+ G GI Q Sbjct: 181 PHELAQHQTISYSYNQSKDEWQFNGPEG-PVSVRVSSRLHVNNGDSCVQAALGGIGITRQ 239 Query: 241 PDFIVNAAIQSGELVELFEGWSTGEQLVVHTLYPTRTHLPPKVQRMRDFLVEQFKVPPW 299 P F+++ ++SG+LV + + E L ++ +YP+R HLP K++ M DFL E F W Sbjct: 240 PTFMIDQYLRSGQLVSILGDYGAPE-LGIYAVYPSRAHLPAKMRAMLDFLTEAFAQVHW 297 >gi|300311458|ref|YP_003775550.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 303 Score = 205 bits (521), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 110/303 (36%), Positives = 174/303 (57%), Gaps = 6/303 (1%) Query: 1 MDKLQAMETFVAVVESGSFVAAVEVTGQSKPAVSRQVSELEQFLGIRLLHRTTRRLSLTD 60 MD+LQ+M F VVE GSFV A E+ S +R +++LE LG RLL+R+TRRLSLT+ Sbjct: 1 MDRLQSMRVFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTE 60 Query: 61 EGRVYFTRCKELLESIQDVESEVGLNSDQACGRLRIGAPQDFGVEQLAPLWGKFTEQNPQ 120 G+ Y R + +L + D E+ V L + + G L I FG QL + +++ P+ Sbjct: 61 AGQAYLERVRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPE 120 Query: 121 ITLNITLSDRTIDLLEEGFDMAIRISTLG-DSRMVARPLAPVRMIACASPAFLQRHGTPV 179 + L+I+LS+R+IDL+EEG D+ S D+ M+ R L R+I C SPA++ R G P Sbjct: 121 VALDISLSERSIDLVEEGIDIGFFSSMQKFDASMIVRQLGVARVILCTSPAYIARQGAPE 180 Query: 180 SPAELRDYPFISYSYRNGGDEWHFKHEHGDTCTVRVRSTVYVNNGATCRAMAVAGQGIAI 239 P +L + +++S+ WH + +G V + S V N+G R +A+AG GI + Sbjct: 181 QPEDLSRHNCLNFSHEYLRHHWHV-NTNGGVLDVPIVSKVVSNSGVLLRDLALAGMGIVL 239 Query: 240 QPDFIVNAAIQSGELVELFEGWSTGEQLVVHTLYPTRTHLPPKVQRMRDFLVEQFKVP-- 297 +P + + ++SG LV++ G+ G Q+++ +YP+R L KV+ +F+ +F P Sbjct: 240 RPSYSLGDDLRSGRLVQVLPGFDMG-QVIMSMVYPSRRLLSAKVRSFAEFISARFPHPEN 298 Query: 298 -PW 299 PW Sbjct: 299 DPW 301 >gi|300312334|ref|YP_003776426.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 302 Score = 196 bits (497), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 105/302 (34%), Positives = 170/302 (56%), Gaps = 2/302 (0%) Query: 1 MDKLQAMETFVAVVESGSFVAAVEVTGQSKPAVSRQVSELEQFLGIRLLHRTTRRLSLTD 60 MD+ + + TF V GS AA G + + R++ LE+ LG++LL RTTR++ LT+ Sbjct: 1 MDQFKQISTFAEVAARGSLSAAARAEGIAPAMIGRRLDALEERLGVKLLQRTTRKIVLTN 60 Query: 61 EGRVYFTRCKELLESIQDVESEVGLNSDQACGRLRIGAPQDFGVEQLAPLWGKFTEQNPQ 120 EG + C+ +L ++D ES V S +A G+L I AP FG + +APL F ++ Sbjct: 61 EGAAFLEDCQRILADLEDAESAVSERSARASGQLLISAPAGFGRQHVAPLLPSFLAEHRD 120 Query: 121 ITLNITLSDRTIDLLEEGFDMAIRISTLGDSRMVARPLAPVRMIACASPAFLQRHGTPVS 180 +TL + L+DR D++ EG D+AIRI+TL DS +V+ LA + A+PA+L+RHGTP S Sbjct: 121 VTLTLNLNDRIADVIGEGIDVAIRIATLTDSNLVSVKLAENHRVIVAAPAYLRRHGTPQS 180 Query: 181 PAELRDYPFISYSYRNGGDEWHFKHEHGDTCTVRVRSTVYVNNGATCRAMAVAGQGIAIQ 240 P EL + ++ S W + + G +V + N+G A+AG+G+A + Sbjct: 181 PDELMRHNCLAMSSEGSQRGWTLR-QSGKVSVYKVGGNMVCNDGEVLHNWALAGRGLAWR 239 Query: 241 PDFIVNAAIQSGELVELFEGWSTGEQLVVHTLYPTRTHLPPKVQRMRDFLVEQFKVPPWC 300 + V AAI++GELV + + ++ ++ ++ R HLP +++ DFL + P + Sbjct: 240 SMWEVGAAIEAGELVTVLDKFAAPAP-SIYAVFAQRRHLPLRIRAFVDFLRHAYAQPDYW 298 Query: 301 PK 302 K Sbjct: 299 KK 300 >gi|300312244|ref|YP_003776336.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 293 Score = 191 bits (485), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 106/288 (36%), Positives = 160/288 (55%), Gaps = 2/288 (0%) Query: 7 METFVAVVESGSFVAAVEVTGQSKPAVSRQVSELEQFLGIRLLHRTTRRLSLTDEGRVYF 66 + TFVAVVE+G+ AA E+ Q+ VSR +S LE LG LL RTTRR+ LT+EGR + Sbjct: 8 LRTFVAVVEAGTLSAAAELLAQTTSGVSRALSRLEDKLGASLLTRTTRRMELTEEGRAFL 67 Query: 67 TRCKELLESIQDVESEVGLNSDQACGRLRIGAPQDFGVEQLAPLWGKFTEQNPQITLNIT 126 + + +L +++ + + L + + G+LR+ A F + + P G+F P I L +T Sbjct: 68 EQARAILAAMESAQDSIRLRTRKPVGKLRVDASAPFMLHCVVPHVGQFRAAYPDIELELT 127 Query: 127 LSDRTIDLLEEGFDMAIRISTLGDSRMVARPLAPVRMIACASPAFLQRHGTPVSPAELRD 186 +DR IDL+E D+AIRI L DS + AR L+ R+ ASPA+L RHG P S L Sbjct: 128 TNDRFIDLVEHRADIAIRIGELQDSSLHARALSSARLNVVASPAYLARHGEPESVEALEQ 187 Query: 187 YPFISYSYRNGGDEWHFKHEHGDTCTVRVRSTVYVNNGATCRAMAVAGQGIAIQPDFIVN 246 + I +S + + W +H GD + R + ++G T R +A+ GQGIA +F+ + Sbjct: 188 HQLIGFSQPDQLNHWPLRHSAGD--RYQTRPAIRASSGETIRHLALQGQGIACLSNFMSD 245 Query: 247 AAIQSGELVELFEGWSTGEQLVVHTLYPTRTHLPPKVQRMRDFLVEQF 294 A I +G LV L +G + + +Y T L ++ DFL E+ Sbjct: 246 ADIAAGRLVRLLPQADSGYRQKISAVYYYSTQLSRRISCFLDFLQEKL 293 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 920,989 Number of extensions: 38563 Number of successful extensions: 268 Number of sequences better than 1.0e-08: 65 Number of HSP's gapped: 206 Number of HSP's successfully gapped: 65 Length of query: 308 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 223 Effective length of database: 1,217,395 Effective search space: 271479085 Effective search space used: 271479085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)