BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0022.p (270 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312191|ref|YP_003776283.1| outer membrane porin [Herbaspir... 453 e-129 gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspir... 209 2e-55 gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) ... 206 2e-54 gi|300310328|ref|YP_003774420.1| porin signal peptide protein [H... 145 5e-36 gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 77 2e-15 >gi|300312191|ref|YP_003776283.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 372 Score = 453 bits (1165), Expect = e-129, Method: Compositional matrix adjust. Identities = 218/270 (80%), Positives = 244/270 (90%) Query: 1 VVGLSGHVGTLTIGRQTDYLEEIGLQYSSFQIFGGGGVRAGHFNSLDRLTGARTSNSLRF 60 VVGL+G GTLT+GRQTDYLEEIG QY+SFQIFGGGGVRA HFNSLDR+TGAR++NSLRF Sbjct: 103 VVGLAGRFGTLTVGRQTDYLEEIGQQYTSFQIFGGGGVRAAHFNSLDRITGARSNNSLRF 162 Query: 61 DTAEMGGFTGSLFYGFGEVAGKTSAGQAFGVGANYRRGNAGIGAAYYQSKLAADTPPSSA 120 DTAE GG GS +YG GEVAG T +GQ+ GVG N+RRGN G+GAAY+QSKLA+D P+ A Sbjct: 163 DTAEYGGLRGSFYYGAGEVAGSTRSGQSLGVGGNFRRGNVGVGAAYFQSKLASDALPARA 222 Query: 121 GDTDLKTFTLGASYQAGPAKLFAAWSQVRRPTQQPLAASGLVAITSATRANVFDVGVDYA 180 GDT+LKTFTLGASYQAGPAKLFAAWSQ+RRPTQQPLA+SGLVAITSATRANV DVGVDYA Sbjct: 223 GDTELKTFTLGASYQAGPAKLFAAWSQIRRPTQQPLASSGLVAITSATRANVLDVGVDYA 282 Query: 181 MNAKLHLLGSVIHDRADLSRASGGATRGHTTQFNVGIDYFFSKRTDLYAMASRQSASDVL 240 +++KLHLL SVIHDRADLSRA+GGATRG TTQ N+G+D SKRTD+YAMASRQ A DVL Sbjct: 283 LDSKLHLLASVIHDRADLSRAAGGATRGSTTQVNLGVDLLLSKRTDVYAMASRQQARDVL 342 Query: 241 NPGVISAAYSSAPADDSSQNVLRIGLRHKF 270 NPG+ISAAYSSAPADDSSQNVLR+GLRHKF Sbjct: 343 NPGIISAAYSSAPADDSSQNVLRVGLRHKF 372 >gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 387 Score = 209 bits (532), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 128/288 (44%), Positives = 176/288 (61%), Gaps = 31/288 (10%) Query: 1 VVGLSGHVGTLTIGRQTDYLEEIGLQYSSFQIFGGGGVRAGHFNSLDRLTGARTSNSLRF 60 VVGLSG GT+ +GRQTD+ + I Y++ FGG V A +SL+RL G RT+NS+ + Sbjct: 113 VVGLSGGFGTVLLGRQTDFADTIS-AYTAVADFGG--VVANSGSSLNRLQGTRTNNSVSY 169 Query: 61 DTAEMGGFTGSLFYGFGEVAGKTSAGQAFGVGANYRRGNAGIGAAYYQSKLAADTPPSS- 119 TA + GFTG+L YGFGE AGKTSAGQA+G+G Y G G+G YYQSK A TP + Sbjct: 170 TTANLSGFTGNLIYGFGEQAGKTSAGQAYGIGGKYDNGPLGLGINYYQSK-AGSTPSDTS 228 Query: 120 ----------------AGDTDLKTFTLGASYQAGPAKLFAAWSQVRRPTQ-QPLAASGLV 162 AG+T LK+ + ASYQ GPA+++A +S+V++ + P A+ + Sbjct: 229 LIPAAGATNANTALGVAGNTALKSLNVVASYQFGPARVYANYSRVKQDLKGTPTVATATL 288 Query: 163 AITSATRANVFDVGVDYAMNAKLHLLGSVIHDRADLSRASGGATRGHTTQFNVGIDYFFS 222 A +A +A+ +++G Y+++ L LL +V H RA +G T+G TQ ++G DYF S Sbjct: 289 A--NAQKADTYEIGTAYSLSPSLKLLAAVDHSRATF---NGVNTKGKLTQISLGADYFLS 343 Query: 223 KRTDLYAMASRQSASDVLNPGVISAAYSSAPADDSSQNVLRIGLRHKF 270 KRTDLYA S ASD+ NPGV A S D+SQ + G+RHKF Sbjct: 344 KRTDLYAFVSNMRASDIQNPGVTGGATGS----DASQTAIITGIRHKF 387 >gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 206 bits (523), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 125/296 (42%), Positives = 176/296 (59%), Gaps = 38/296 (12%) Query: 1 VVGLSGHVGTLTIGRQTDYLEEIGLQYSSFQIFGGGGVRAGHFNSLDRLTGARTSNSLRF 60 VVGL+G+ G++ +GRQTD+ + I Y+S Q FGG AGH +LDRL G RT+NS+ + Sbjct: 107 VVGLAGNFGSVLLGRQTDFADTIS-SYTSVQDFGGWTGAAGH--NLDRLEGTRTNNSISY 163 Query: 61 DTAEMGGFTGSLFYGFGEVAGKTSAGQAFGVGANYRRGNAGIGAAYYQSKLAADTPPSSA 120 TA + GFTG+L YGFGE AG+TSAGQAFGVG Y G G+G YYQSK A TP ++ Sbjct: 164 TTANLNGFTGNLMYGFGETAGQTSAGQAFGVGGKYDNGPLGLGINYYQSK-AGSTPADAS 222 Query: 121 ----------------GDTDLKTFTLGASYQAGPAKLFAAWSQVRRP--TQQPLAASG-- 160 G T K + ASYQ GPA+++ WS+V++ TQ L A+G Sbjct: 223 LLAATGTNQFTNGTNNGSTAQKVLNVAASYQFGPARVYGNWSRVKQDLNTQANLTAAGVG 282 Query: 161 -LVAITSAT-----RANVFDVGVDYAMNAKLHLLGSVIHDRADLSRASGGATRGHTTQFN 214 L I++ T +A+++++G Y+++ L LL +V H RA SG + TQ + Sbjct: 283 SLATISNRTLAASNKADIYEIGTAYSLSPSLKLLAAVDHTRAQFDGVSG---KAKLTQVS 339 Query: 215 VGIDYFFSKRTDLYAMASRQSASDVLNPGVISAAYSSAPADDSSQNVLRIGLRHKF 270 +G DY+ SKRTDLYA + A+D+ NPGV + + D +Q + +G+RHKF Sbjct: 340 LGTDYWLSKRTDLYAFLAYSKATDLNNPGV-----ADSSGTDGNQGAVAVGIRHKF 390 >gi|300310328|ref|YP_003774420.1| porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 405 Score = 145 bits (365), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 106/312 (33%), Positives = 148/312 (47%), Gaps = 53/312 (16%) Query: 1 VVGLSGHVGTLTIGRQTDYLEEIGLQYSSFQIFGGGGVRAG-HFNSLDRLTGARTSNSLR 59 VGLSG+ GT+ +GRQ D L +IG S+ GG GV H LDR G R +NS+R Sbjct: 105 TVGLSGNFGTVLVGRQNDPLNDIGGIASA----GGFGVPGTVHSKGLDRTDGQRLNNSIR 160 Query: 60 FDTAEMGGFTGSLFYGFGEVAGKTSAGQAFGVGANYRRGNAGIGAAYYQSKLAADTPPSS 119 +++ G + S YGFGE G+ G++ G+G+ Y G +G Y+QSK+ + SS Sbjct: 161 YNSPNFNGLSFSGIYGFGEQVGRAVGGRSIGLGSQYVNGPLSLGLGYFQSKMGGGSDGSS 220 Query: 120 ----------------AGDTDLKTFTLGASYQAGPAKLFAAWSQVRRPTQQ--------- 154 AGDT LKT+TL A+YQ GPAK++ A+S VR P + Sbjct: 221 DVNAKAACNGAGEKDHAGDTCLKTWTLAAAYQFGPAKVYGAFSHVRLPLAKLHTKVRKDN 280 Query: 155 ---------------PLAASGL-VAITSATRANVFDVGVDYAMNAKLHLLGSVIHDRADL 198 P A+ + + + FD+G+ Y + L L+ +V H RA Sbjct: 281 NFGAMYDAKTGTGYNPSDATAFSIGGANHKSSQTFDIGMSYQLMPSLTLMSAVQHTRAKF 340 Query: 199 SRASGGATRGHTTQFNVGIDYFFSKRTDLYAMASRQSASDVLNPGVISAAYSSAPADDSS 258 GA G Q N+G Y SKRT YA+ +SD+ N G+ + P D S Sbjct: 341 V----GAASGRLLQLNLGAKYDLSKRTSAYALVRNLRSSDMYNVGL---SKDQEPGIDRS 393 Query: 259 QNVLRIGLRHKF 270 Q + GL H F Sbjct: 394 QTAISTGLIHTF 405 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 76.6 bits (187), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 80/283 (28%), Positives = 121/283 (42%), Gaps = 49/283 (17%) Query: 2 VGL-SGHVGTLTIGRQTDYLEEIGLQYSSFQI-----FGGG-GVRAGHFNSLDR--LTGA 52 VGL S G+L++GRQ D++ IG QY+ + FG G A H +L+ G Sbjct: 105 VGLGSTQWGSLSVGRQYDFMANIGGQYAMGALSPAGSFGWGLHADAAHGTALNNHIYAGD 164 Query: 53 RTSNSLRFDTAEMGGFTGSLFYGFGEVAGKTSAGQAFGVGANYRRGNAGIGAAYYQSKLA 112 RT+NS+++++A+ GGF+ G GE AG +AG+ Y G + LA Sbjct: 165 RTNNSIKYNSAKFGGFSFGAMVGLGETAGNNNAGRTVSATGQYDNGP-------FSGALA 217 Query: 113 ADTPPSSAGDTDLKTFTLGASYQAGPAKLFAAWSQVRRPTQQPLAASGLVAITSATRANV 172 S G + + GA YQ G F +QV+ ++ +A Sbjct: 218 YTDIKDSTGSFSTRIYGGGARYQIGAFTPFGVVTQVKN-------------TNTSAKATT 264 Query: 173 FDVGVDYAMNAKLHLLGSVIHDRADLSRASGGATRGHTTQFNVGIDYFFSKRTDLYAMAS 232 +++G YA+ L + + R G G+ +DY SKRTDLY A Sbjct: 265 YELGTVYALTPLWDLAAAYQFQK----RNQG---VGNAQALVATVDYKISKRTDLYVGAV 317 Query: 233 RQ-----SASDVLNPGVISAAYSSAPADDSSQNVLRIGLRHKF 270 +A V G+ SA +Q LR+G+RH+F Sbjct: 318 YDRDKGYNAYPVFGGGIQSAT--------GNQTALRLGMRHRF 352 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 861,559 Number of extensions: 38101 Number of successful extensions: 158 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 128 Number of HSP's successfully gapped: 11 Length of query: 270 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 186 Effective length of database: 1,222,130 Effective search space: 227316180 Effective search space used: 227316180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)