BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0019.p (229 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312825|ref|YP_003776917.1| NAD-dependent aldehyde dehydrog... 449 e-128 gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 136 1e-33 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 136 1e-33 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 134 7e-33 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 132 2e-32 >gi|300312825|ref|YP_003776917.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 476 Score = 449 bits (1156), Expect = e-128, Method: Compositional matrix adjust. Identities = 219/229 (95%), Positives = 224/229 (97%) Query: 1 MELGGNDAAIVLEDADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHDRIVAELKAII 60 MELGGNDAAIVL+DADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHD+IV ELKAII Sbjct: 248 MELGGNDAAIVLDDADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHDQIVDELKAII 307 Query: 61 AATPMGDGLQPGIAMGPVQNPMQLKKVAALVESAREEGATIVCGGVLPDGPGNFYPLTLV 120 AA PMGDGLQPGIAMGPVQNPMQLKKV ALVESAREEG TIVCGGVLPDGPGNFYPLTLV Sbjct: 308 AALPMGDGLQPGIAMGPVQNPMQLKKVVALVESAREEGGTIVCGGVLPDGPGNFYPLTLV 367 Query: 121 TDLRDGARLVDEEQFGPVLPIIRYSDLEQAIANANRLDVGLGASVWSSDTQRAAEVATRL 180 T LRDGARLVDEEQFGPVLPIIRYSDLEQAIANANRLDVGLGASVWSSD+QRAA+VATRL Sbjct: 368 TGLRDGARLVDEEQFGPVLPIIRYSDLEQAIANANRLDVGLGASVWSSDSQRAAQVATRL 427 Query: 181 QAGTVWVNQHGMVHPMVPFGGNKRSGWGLEFGQEGLKGVTQPQVISLKK 229 QAGTVWVNQHGMVHPMVPFGGNKRSGWG+EFGQEGLKGVTQPQVISLKK Sbjct: 428 QAGTVWVNQHGMVHPMVPFGGNKRSGWGVEFGQEGLKGVTQPQVISLKK 476 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 136 bits (343), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 80/225 (35%), Positives = 109/225 (48%) Query: 1 MELGGNDAAIVLEDADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHDRIVAELKAII 60 +ELGG A I+ EDAD + A G F GQ+C RL V ++DR V + A Sbjct: 264 LELGGKSANIIFEDADLRLAAFGAQAAIFSGAGQSCVAGSRLLVQRKVYDRFVETVAAGA 323 Query: 61 AATPMGDGLQPGIAMGPVQNPMQLKKVAALVESAREEGATIVCGGVLPDGPGNFYPLTLV 120 +G L +GP+ N Q + + +V E GAT+ G G F TL+ Sbjct: 324 EKIRLGAPLDDSTEVGPINNRKQYEHIQRMVARGLEAGATLAAGHTRYGEEGYFVRPTLL 383 Query: 121 TDLRDGARLVDEEQFGPVLPIIRYSDLEQAIANANRLDVGLGASVWSSDTQRAAEVATRL 180 + + E FGPV I + D E+AIA AN + GL +VW+ D RA VA + Sbjct: 384 AHASNAMEVARSEIFGPVAVAIPFEDEEEAIAIANDSEFGLAGAVWTRDVARAHRVAASV 443 Query: 181 QAGTVWVNQHGMVHPMVPFGGNKRSGWGLEFGQEGLKGVTQPQVI 225 AGT WVN + ++ PFGG RSG+G G E L TQ + + Sbjct: 444 NAGTFWVNSYKTINVASPFGGFNRSGYGRSSGMEALYDYTQTKSV 488 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 136 bits (343), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 76/226 (33%), Positives = 119/226 (52%) Query: 1 MELGGNDAAIVLEDADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHDRIVAELKAII 60 MELGG+ A+V +DAD A L F N GQ C R V + + V + Sbjct: 248 MELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFVTYA 307 Query: 61 AATPMGDGLQPGIAMGPVQNPMQLKKVAALVESAREEGATIVCGGVLPDGPGNFYPLTLV 120 +G+GL+ GI MGPV N ++ + AL++ A +GA + GG G F+ T++ Sbjct: 308 EQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQPTVL 367 Query: 121 TDLRDGARLVDEEQFGPVLPIIRYSDLEQAIANANRLDVGLGASVWSSDTQRAAEVATRL 180 T+L AR ++EE FGP+ I + L++ I ANRL GL A ++ +A R+ Sbjct: 368 TNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHALAARV 427 Query: 181 QAGTVWVNQHGMVHPMVPFGGNKRSGWGLEFGQEGLKGVTQPQVIS 226 ++G + +N G+ P +PFGG K SG+G E G E ++ ++++ Sbjct: 428 ESGMLTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLVT 473 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 134 bits (337), Expect = 7e-33, Method: Compositional matrix adjust. Identities = 75/216 (34%), Positives = 115/216 (53%) Query: 1 MELGGNDAAIVLEDADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHDRIVAELKAII 60 +ELGGN IV +DAD A G + N GQTC CA R+YV D ++D A+L + Sbjct: 255 LELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEAV 314 Query: 61 AATPMGDGLQPGIAMGPVQNPMQLKKVAALVESAREEGATIVCGGVLPDGPGNFYPLTLV 120 +G G + G+ GP+ N ++KV V A +GA ++ GG +F+ T++ Sbjct: 315 KKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFEPTVL 374 Query: 121 TDLRDGARLVDEEQFGPVLPIIRYSDLEQAIANANRLDVGLGASVWSSDTQRAAEVATRL 180 D+ ++ EE FGP+ P+ R+ E+ +A AN + GL + +S D R VA L Sbjct: 375 ADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRVAEGL 434 Query: 181 QAGTVWVNQHGMVHPMVPFGGNKRSGWGLEFGQEGL 216 ++G V +N + + + PFGG K+SG G E G+ Sbjct: 435 ESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGI 470 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 132 bits (333), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 83/227 (36%), Positives = 117/227 (51%) Query: 1 MELGGNDAAIVLEDADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHDRIVAELKAII 60 +ELGG IV +DA + G G F+N GQ CA R+ V +L R L Sbjct: 254 LELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQRFAKALAEAA 313 Query: 61 AATPMGDGLQPGIAMGPVQNPMQLKKVAALVESAREEGATIVCGGVLPDGPGNFYPLTLV 120 A +GD QPG+ MGPV Q ++V ++ +EGAT++ GGV G F T+ Sbjct: 314 AGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVSRPDKGWFVQPTIF 373 Query: 121 TDLRDGARLVDEEQFGPVLPIIRYSDLEQAIANANRLDVGLGASVWSSDTQRAAEVATRL 180 D +G + EE FGPV II + E+AIA AN GL A+VW+++ RA VA + Sbjct: 374 ADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTTNLARAHRVAAGV 433 Query: 181 QAGTVWVNQHGMVHPMVPFGGNKRSGWGLEFGQEGLKGVTQPQVISL 227 + G V VN + +P+GG K SG G E G G T+ + +++ Sbjct: 434 KVGAVGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTVTV 480 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.137 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 820,189 Number of extensions: 35486 Number of successful extensions: 134 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 114 Number of HSP's successfully gapped: 19 Length of query: 229 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 147 Effective length of database: 1,231,600 Effective search space: 181045200 Effective search space used: 181045200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)