BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0010.0010.p (447 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312188|ref|YP_003776280.1| hemolysin secretion-related tra... 644 0.0 gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transm... 146 4e-36 gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD ... 108 1e-24 >gi|300312188|ref|YP_003776280.1| hemolysin secretion-related transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 644 bits (1660), Expect = 0.0, Method: Compositional matrix adjust. Identities = 325/447 (72%), Positives = 386/447 (86%) Query: 1 MSLHHRSRAWLALWQRYGRVLRHAWRQRDPRKSGFYNEQEAEFLPAALSLQESPDSASLR 60 MSL HR +AW LW+RY RV HAWRQR +S F+N+ EAEFLPAALSLQE+PDS SLR Sbjct: 1 MSLRHRWQAWTDLWRRYLRVFGHAWRQRGHYRSDFFNKDEAEFLPAALSLQEAPDSRSLR 60 Query: 61 WTARLLMAMVLLTLLWSLVGHIDIVVNATGKVIPSARIKTIASIEVASVRDILVREGQRV 120 WTARLL+ MV+ LLWSL+G IDIVV+A GKVIPSAR KTIAS++V++VR ILV EGQ V Sbjct: 61 WTARLLVGMVIFALLWSLLGRIDIVVSANGKVIPSARTKTIASVDVSAVRAILVSEGQLV 120 Query: 121 RVGQILMELDATSSDAERDKAADAVAQARLQVARAAAMMQAVQELQAPQLAEVGGAGRAQ 180 + G++L+ELD++SSDAERDKAADAVAQARLQ ARA AMM+A+Q L+ P+LA+V G +AQ Sbjct: 121 KAGEVLIELDSSSSDAERDKAADAVAQARLQAARAQAMMEAIQNLRTPELAQVPGVAQAQ 180 Query: 181 RESAERQLQGQYQDFRARLARIDDEITRYAAALQLASARATDYKELMADNTVSRHAWLEK 240 E+ QL QYQDFRARL R+DDEI+RY AAL+LA+ RA+DY+ L+AD+TVSRHAWLEK Sbjct: 181 WEAVRSQLVAQYQDFRARLGRLDDEISRYGAALKLATQRASDYQTLVADHTVSRHAWLEK 240 Query: 241 EQARVDLQGQLSEARNQRTALLAQTQKEAHDSRIEAEKIIESAQQDQRRAEERSRLLTLM 300 EQARVDLQGQL +ARNQR L+AQT+K+AHDSRIEA+K IE++QQDQRRA+E S+LL L+ Sbjct: 241 EQARVDLQGQLDDARNQRATLVAQTRKDAHDSRIEADKTIEASQQDQRRADEHSKLLKLV 300 Query: 301 APVSGTVQQLAVHTVGGVVAAAQPLMQIVPEEGDIEIEAWLENKDVGFVRIGQEVEVKID 360 APV+GTVQQL VHT+GGVV AAQPLMQIVP++ IE+EA LENKDVGFV IGQ EVKID Sbjct: 301 APVTGTVQQLTVHTIGGVVPAAQPLMQIVPQDSQIEVEAMLENKDVGFVEIGQAAEVKID 360 Query: 361 AFDYTKYGTVPARVVHVSRDAIQDEKKGLVYSARILLSRNSLNVDGRSLPLSAGLAVNVG 420 AFDYTKYGT+PARVVH+SRDAIQDEKKGL+YSARI+L ++SL VDGR + LSAG+A NV Sbjct: 361 AFDYTKYGTLPARVVHISRDAIQDEKKGLLYSARIVLDQSSLIVDGRKVQLSAGMAANVE 420 Query: 421 IRTGSRRVIEYVLSPLMRHQKEALHER 447 I+TG+RRVIEY LSPL+RHQKEALHER Sbjct: 421 IKTGNRRVIEYALSPLIRHQKEALHER 447 >gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 386 Score = 146 bits (368), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 112/397 (28%), Positives = 196/397 (49%), Gaps = 29/397 (7%) Query: 58 SLRWTARLLMAMVLLTLLWSLVGHIDIVVNATGKVIPSARIKTIASIEVASVRDILVREG 117 S +W RL MA VL+ LLW+ V ID V A G+VI +R +++ + + V+ ILV+EG Sbjct: 12 SAQWVLRLSMASVLVLLLWAAVSQIDQVTRAQGQVIAVSRTQSVQAPDGGPVKQILVKEG 71 Query: 118 QRVRVGQILMELDATSSDAERDKAADAVAQARLQVARAAAMMQAVQELQAPQLAEVGGAG 177 + V+ GQ+LM L+ + + VA R+ +AR A + P L + Sbjct: 72 EAVKQGQLLMVLEQDRVQTSLNDSRAKVAALRISLARLRAEVYGHPLNFEPDLLDY---- 127 Query: 178 RAQRESAERQLQGQYQDFRARLARIDDEITRYAAALQLASARATDYKELMADNTVSRHAW 237 + + Q + R ID ++ L LA+ K L V R Sbjct: 128 -------KEYIANQKDLYVKRKILIDQDLASLQDMLGLANQELEMNKSLERTGDVGRADV 180 Query: 238 LEKEQARVDLQGQLSEARNQRTALLAQTQKEAHDSRIEAEKIIESAQQDQRRAEERSRLL 297 L ++ D++ Q++ RN K ++ ++ K+ E + + E+R+++L Sbjct: 181 LRLQRNVADIRAQINNKRN----------KYFQEALVDMTKVQEDLNTQREQLEDRTQVL 230 Query: 298 ---TLMAPVSGTVQQLAVHTVGGVVAAAQPLMQIVPEEGDIEIEAWLENKDVGFVRIGQE 354 L AP G V+ + V T+GGVV + +M+I+P + I +EA + D +V++GQ Sbjct: 231 EHTELKAPADGVVKNIKVTTIGGVVRSGDVVMEILPVDNLI-VEAKVSTADSAYVKVGQA 289 Query: 355 VEVKIDAFDYTKYGTVPARVVHVSRDAIQDE-KKGLVYSARILLSRNSLNVDGR---SLP 410 VK+DA+DY+ YGT+ VV+VS D + DE ++G + R+ ++ GR ++ Sbjct: 290 ANVKLDAYDYSIYGTLNGHVVYVSPDTLLDETRQGAMPYYRVHIALEEAVFKGRKADAIQ 349 Query: 411 LSAGLAVNVGIRTGSRRVIEYVLSPLMRHQKEALHER 447 + G+ V I+ +R V+ ++ P+ + +E++ ER Sbjct: 350 VKPGMTATVEIKANTRTVLSFLTKPIAKTLQESMGER 386 >gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD family secretion protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 108 bits (269), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 108/399 (27%), Positives = 180/399 (45%), Gaps = 29/399 (7%) Query: 76 WSLVGHIDIVVNATGKVIPSARIKTIASIEVASVRDILVREGQRVRVGQILMELDATSSD 135 WS+ ID+VV +G+++ S + I +E V ILVREG V+ GQ L+ + ++ Sbjct: 58 WSVFAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAAVKKGQTLISISDVRAN 117 Query: 136 AERDKAADAVAQARLQVARAAAMMQAVQELQAPQLAEVGG-------AGRAQRESAERQL 188 A+ + + R ++AR +A LQ P E G A A R S Q Sbjct: 118 ADLGEGRVKILGLRAKIARLSAEASGASSLQMPDDLERGDPAVQSEQAAFAARSSKLMQE 177 Query: 189 QGQYQDFRA-RLARIDDEITRY---AAALQLASARATDYKELMADNTVSRHAWLEKEQAR 244 ++ A R A + + I+R A+ L+LA + +++ N S+ ++ Sbjct: 178 MSVLREQSAQRQAELGEAISRQKSQASELELAQRQYKLIHDMLVRNAASQLEDIQAASRV 237 Query: 245 VDLQGQLSEAR------NQRTALLAQTQKEA-----HDSRIE---AEKIIESAQQDQRRA 290 D Q +L+ N A Q EA D+R E AE + Q++ + Sbjct: 238 QDAQSKLAATNAMIPRLNAAIAEAQAKQGEAASRFRSDARTELTAAETELARLQEEIKSR 297 Query: 291 EERSRLLTLMAPVSGTVQQLAVHTVGGVVAAAQPLMQIVPEEGDIEIEAWLENKDVGFVR 350 +R + AP+ G V ++ ++T+GGVV P++++ P + + IEA + D + Sbjct: 298 NDRVSRSEVKAPMDGVVNRVMINTIGGVVRPGDPIIEMTPSDDKLVIEARISPTDRAELV 357 Query: 351 IGQEVEVKIDAFDYTKYGTVPARVVHVSRDAIQDE---KKGLVYSARILLSRNSLNVDGR 407 G VK+ A+DY YG + V +S D + DE + Y +I + R + + Sbjct: 358 TGARARVKVSAYDYGVYGAMDGVVTEISADTVPDEGSPRGERFYRLKIEVKRGHDELQ-Q 416 Query: 408 SLPLSAGLAVNVGIRTGSRRVIEYVLSPLMRHQKEALHE 446 L L G+ + I G R V +Y++SPL R + AL E Sbjct: 417 KLALMPGMTASADIIVGRRTVWQYLMSPLSRFGQGALRE 455 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.130 0.359 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,118,365 Number of extensions: 37630 Number of successful extensions: 224 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 224 Number of HSP's successfully gapped: 6 Length of query: 447 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 359 Effective length of database: 1,203,190 Effective search space: 431945210 Effective search space used: 431945210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)