BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0001.p (754 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309449|ref|YP_003773541.1| TonB-dependent hemoglobin/trans... 1283 0.0 gi|300312129|ref|YP_003776221.1| TonB-dependent ferric sideropho... 66 1e-11 gi|300309512|ref|YP_003773604.1| TonB-dependent heme/hemoglobin ... 57 7e-09 >gi|300309449|ref|YP_003773541.1| TonB-dependent hemoglobin/transferrin/lactoferrin receptor protein [Herbaspirillum seropedicae SmR1] Length = 765 Score = 1283 bits (3321), Expect = 0.0, Method: Compositional matrix adjust. Identities = 629/754 (83%), Positives = 687/754 (91%), Gaps = 3/754 (0%) Query: 1 VFLALQQWLCASAFAQSADANTQANPARASASESITLAQASGKKLQDVVVTANRSGSSDL 60 VFLALQQWLC A+AQ A+ + +S+ S+ LAQASGKKLQ+VVVTANRSGSSD Sbjct: 15 VFLALQQWLCVGAYAQ---ADQPQSVQSSSSDPSVVLAQASGKKLQEVVVTANRSGSSDS 71 Query: 61 DHAAATVSVITSEEIEEHNAKDIKDALRYEPGVEVRRSVYRMSGITGATATTGRGGNEGI 120 DHAAATVSVI++EEIE+HNA+DIKDALRYEPGVEV+RSVYRMSGITG ATTGRGGNEGI Sbjct: 72 DHAAATVSVISAEEIEQHNARDIKDALRYEPGVEVKRSVYRMSGITGPGATTGRGGNEGI 131 Query: 121 TIRGLDGNRVLMLEDGIVMPRAFSQGVASVGRGATTDTELYRRIEVLRGPASSLYGSDGL 180 +IRGLDGNRVLMLEDGI MPR+FS G +SVGRGATTDTELYRRIEVLRGPASSLYGSDGL Sbjct: 132 SIRGLDGNRVLMLEDGISMPRSFSYGTSSVGRGATTDTELYRRIEVLRGPASSLYGSDGL 191 Query: 181 TGAVNFITKDPSDLLDVYGKSSYFSFKPSYDSVDKSFGATASAAWGSQQWQGMLILNARH 240 TGAVNFITKDP+DLL+V+GKSSYFSFKPSYDS D S GA+ASAAWG QQWQGMLILN RH Sbjct: 192 TGAVNFITKDPADLLEVFGKSSYFSFKPSYDSGDHSSGASASAAWGGQQWQGMLILNTRH 251 Query: 241 GNESDNKGTNDVRGSARTTPDPMSTTNRSALGKLVFKPNGIDTFKLTLQATEERESGDSL 300 G+++DNKG+NDV G+ARTTPDPM RSALGKLVF+PNG+D FKLTLQATE+R+SGDSL Sbjct: 252 GSQADNKGSNDVLGTARTTPDPMHYNTRSALGKLVFRPNGVDLFKLTLQATEQRDSGDSL 311 Query: 301 SAIGNGVTAYSTSSKSTGQRATLAYERNDADQPWIQKFKASLTYRNADTDQLTNEAGTST 360 SA+ NGVT Y TSSKSTGQRATLAYE DA+ PW+QK KASLTYRNADTDQ T EAG S Sbjct: 312 SALTNGVTQYHTSSKSTGQRATLAYEHVDAEHPWVQKIKASLTYRNADTDQYTYEAGASA 371 Query: 361 ATIVRPRFRAVSYSDQMISGSLLAESNFGSDSWRHKLVYGLDLSLSTLQTSADGTGWTSC 420 +IVRPRFRAV+Y D+M +GSLLAESNFG DSWRHKLVYGLDLSLSTLQ +A+GTGW +C Sbjct: 372 GSIVRPRFRAVAYQDRMSAGSLLAESNFGPDSWRHKLVYGLDLSLSTLQVNANGTGWNTC 431 Query: 421 TGTQYCQYFPKTESTQLGAYVQDEMRTGALTTIAGLRLDSYELKPKASAKYDAQAIANGQ 480 TGTQYCQYFPKTES LG Y+QDEMRTGALTTIAGLR DSYELKP+ASAKYDAQAIANGQ Sbjct: 432 TGTQYCQYFPKTESNLLGVYLQDEMRTGALTTIAGLRYDSYELKPQASAKYDAQAIANGQ 491 Query: 481 PAVSSRDSAFSPRLAFLYEVTPAFIPYIQYARGFRSPSPQEINSFFNNTVYRQISNPDLK 540 PAVSSRDSA SPRLA LYEVTPAFIPYIQYARGFRSPS QE+NSFFNNTVYRQ+SNPDLK Sbjct: 492 PAVSSRDSALSPRLALLYEVTPAFIPYIQYARGFRSPSAQEVNSFFNNTVYRQVSNPDLK 551 Query: 541 PETSNTVEVGLRGKLAAGPGALRYSAAAYKGNYRNFIDLVTVRGNLTVANPTIFQYRNAA 600 PETSNTVE+GLRGKLA G GALRYSAAAYKGNY NFIDLVTV GNLT+ANPTIFQYRNAA Sbjct: 552 PETSNTVELGLRGKLALGAGALRYSAAAYKGNYSNFIDLVTVGGNLTMANPTIFQYRNAA 611 Query: 601 KAEIHGLEGRIDYALDNGFTVKAGFAWTKGTTTTNGVEQGLESVAPLSVVAGLRYERAQQ 660 KAEIHGLEGR+DY +D GF++K+GFAWTKG+TT+NG+E+GLESVAPLSVV GLRYER+QQ Sbjct: 612 KAEIHGLEGRLDYQMDIGFSLKSGFAWTKGSTTSNGLERGLESVAPLSVVTGLRYERSQQ 671 Query: 661 WFVQSDLVYNAAKKKEDIPTATNFVSPSFFVMDLSGGYHLTRNMTVTAGIRNLFDRKYWS 720 F QSDLVYNAAKKKEDIPTATNFVSP+FFVMD+SGGYHL RNMT+ AGIRNLFDRKYWS Sbjct: 672 GFFQSDLVYNAAKKKEDIPTATNFVSPAFFVMDISGGYHLNRNMTLYAGIRNLFDRKYWS 731 Query: 721 WTDIRGLSLADSAVNKDAYTEPGRSFNVSLKIAY 754 WTDIRGLSLADSAVNKDAYTEPGRSFN+SLKI Y Sbjct: 732 WTDIRGLSLADSAVNKDAYTEPGRSFNLSLKIEY 765 >gi|300312129|ref|YP_003776221.1| TonB-dependent ferric siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 796 Score = 65.9 bits (159), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 131/553 (23%), Positives = 218/553 (39%), Gaps = 118/553 (21%) Query: 60 LDHAAATVSVITSEEIEEHNAKDIKDALRYEPGVEVRRSVYRMSGITGATATTGRGGNEG 119 L +VSVI + I+ +A+ + D LR GV R Y G Sbjct: 167 LSETPKSVSVINAAVIQSQSAQTLSDVLRNASGVVARPGPY---------------GVPS 211 Query: 120 ITIRGLDGNRVLMLEDGIVMPRAFSQGVASVGRGAT----TDTELYRRIEVLRGPASSLY 175 IRG GN +M S G++S G A+ T T +EV++GP++ + Sbjct: 212 FAIRGYVGNAPVM-----------SDGLSSYGSAASPAALTPTIAIAGVEVVKGPSAIMA 260 Query: 176 GSDGLTGAVNFITKDP-----SDLLDVYGKSSYFSFKPSYDS---VDKS----FGATASA 223 G VN I K P ++ YG +S+ ++DS +D+ + SA Sbjct: 261 ADAPPGGVVNVIKKAPQVEPFHEVQVAYGSNSFSQV--AFDSTGAIDEDKRLRYRFVVSA 318 Query: 224 AWGSQQWQGMLILNARH-----GNESDNKGTNDVRGSARTT---PDPMSTTNRSALGKL- 274 S+ + G RH + ++ T+ G A T P P T + G + Sbjct: 319 DKSSENFMGYD--GGRHFYIAPTLQWKDRSTDLTLGYAHTVTREPFPQYTVGYARGGYVD 376 Query: 275 --VFKPNGI--DTFKLT---LQATEERESGDSLSAIG-NGVTAYSTSSKSTGQRATLAYE 326 V P G D F +T L A E++ D+L+ + NG T ST+ G A A Sbjct: 377 GYVEHPLGNRGDHFSMTADELTAKLEQKLSDNLTFVSKNGYT--STTQGQDGWAA--AST 432 Query: 327 RNDADQPWIQKFKASLTYRNADTDQL----TNEAGTSTATIVRPRFRAVSYSDQMISGSL 382 +++ + FK+S Y A++ ++ AG + T+V V YS Sbjct: 433 VTNSNALTLLGFKSSANYYTANSQNYLRLKSDLAGVKSTTLV-----GVDYS-------- 479 Query: 383 LAESNFGSDSWRHKLVYGLDLSLSTLQTSADGTGWTSCTGTQYCQYFPKTESTQLGAYVQ 442 + H + Y + S +T GT + + F ++ ++G+Y+Q Sbjct: 480 ----------YMHYVQYDRNSSSTTTIPDIFGTINVAPISSPLTYQF-GGQTQRVGSYLQ 528 Query: 443 DEMRTGALTTIAGLRLDSYELKPKASAKYDAQAIANGQPAVSSRDSAFSPRLAFLYEVTP 502 +++ LT +A +R DSY + I P+ +A+SP L +Y++TP Sbjct: 529 EQLIYSNLTVLASIRRDSY---------LSSTNIVGRAPSGGMHQTAYSPSLGLMYQLTP 579 Query: 503 AFIPYIQYARGFRSPSPQEINSFFNNTVYRQISNPDLKPETSNTVEVGLRGKLAAGPGAL 562 PY+ + +GF+ P + ++ L P+ S E G++ L L Sbjct: 580 EIAPYVNWNKGFQ---PNNVTAYGGGL---------LAPKQSEQFEAGVKFNLMD--DKL 625 Query: 563 RYSAAAYKGNYRN 575 + A Y +Y N Sbjct: 626 ELTTALYHIDYTN 638 >gi|300309512|ref|YP_003773604.1| TonB-dependent heme/hemoglobin receptor protein [Herbaspirillum seropedicae SmR1] Length = 690 Score = 56.6 bits (135), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 68/238 (28%), Positives = 107/238 (44%), Gaps = 46/238 (19%) Query: 48 VVVTANRSGSSDLDHAAATVSVITSEEIEEHNAKDIKDALRYEPGVEVRRSVYRMSGITG 107 +VVTA GS + VI +E+ + + L E GV Sbjct: 58 IVVTATPFGSQEDVQVLTPAKVIAGDELRNKLGNSLGETLSSELGV-------------- 103 Query: 108 ATATTGRGGNEGITIRGLDGNRVLMLEDGIVMPRAFSQGVASVG-----RGATTDTELYR 162 + + G G + I IRG++G RV++L++G+ GV+ V T+ R Sbjct: 104 SASAFGAGASRPI-IRGMEGPRVMILQNGM--------GVSDVSTVSNDHAVATEAATAR 154 Query: 163 RIEVLRGPASSLYGSDGLTGAVNFIT-KDPSDLLDVYGKSSYFSFKPSYDSVD----KSF 217 +IE+LRGPA+ LYGS + G VN + + P++L+ GK + + + Y +VD SF Sbjct: 155 QIEILRGPAALLYGSGAIGGLVNVVNDRIPTELV---GKPT-GTVEGRYGTVDGEKNTSF 210 Query: 218 GATASAAWGSQQWQGMLILNARHGNESDNKGTNDVR---GSARTTPDPMSTTNRSALG 272 SA Q L L+ + + SD K + R GS + P S T+ S++G Sbjct: 211 SLDTSAG------QIGLHLDGNYRDTSDYKIPGNARQGDGSTASGRLPNSFTHESSVG 262 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.129 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,249,011 Number of extensions: 92463 Number of successful extensions: 288 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 291 Number of HSP's successfully gapped: 3 Length of query: 754 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 662 Effective length of database: 1,184,250 Effective search space: 783973500 Effective search space used: 783973500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 134 (56.2 bits)