BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0175.p (415 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312971|ref|YP_003777063.1| acyl-CoA transferases/carnitine... 825 0.0 gi|300312108|ref|YP_003776200.1| acyl-CoA transferases/carnitine... 152 3e-38 gi|300310600|ref|YP_003774692.1| acyl-CoA transferases/carnitine... 74 3e-14 >gi|300312971|ref|YP_003777063.1| acyl-CoA transferases/carnitine dehydratase [Herbaspirillum seropedicae SmR1] Length = 415 Score = 825 bits (2130), Expect = 0.0, Method: Compositional matrix adjust. Identities = 398/415 (95%), Positives = 410/415 (98%) Query: 1 MSTATSKPGPLAGIKVLELGTLIAGPFCSRMLAEFGAEVIKIEAPGDGDPLRHWRVLKDG 60 M+T TSKPGPLAGIKVLELGTLIAGPFCSRMLAEFGA+VIK+EAPG+GDPLR WRVLKDG Sbjct: 1 MTTGTSKPGPLAGIKVLELGTLIAGPFCSRMLAEFGADVIKVEAPGNGDPLRQWRVLKDG 60 Query: 61 TSLWWSVQARNKKSITLNMKDERAQQIARQLALDADVIIENYRPGVLEKWKLGYEDLKQL 120 TSLWWSVQARNKKSITLNMKDERAQ+IAR+LAL+ADVIIENYRPGVLEKWKLGYEDLKQL Sbjct: 61 TSLWWSVQARNKKSITLNMKDERAQEIARKLALEADVIIENYRPGVLEKWKLGYEDLKQL 120 Query: 121 NPATIMVRLSGYGQTGPLRDLPGFGAIGESMGGIRYVSGHPDRPPVRIGISIGDSIAALH 180 NPATIMVRLSGYGQTGPLRDLPGFGAIGESMGGIRYVSGHPDRPPVRIGISIGDSIAALH Sbjct: 121 NPATIMVRLSGYGQTGPLRDLPGFGAIGESMGGIRYVSGHPDRPPVRIGISIGDSIAALH 180 Query: 181 GVIGAMMALRHRDVSGGRWNGKSGADCVAGQGQMVDVALYEAVFNMMESMVPEFDFAGVV 240 GVIGAMMALRHRDV+GGRWNGK GADCVAGQGQMVDVALYEAVFNMMESMVPEFDFAGVV Sbjct: 181 GVIGAMMALRHRDVTGGRWNGKQGADCVAGQGQMVDVALYEAVFNMMESMVPEFDFAGVV 240 Query: 241 RERTGGALPGIVPSNTYTTGEGMNIVIAGNGDAIFKRLMSAIGRDDMANDPGLARNDGRV 300 RERTGGALPGIVPSNTYTTGEGMNIVIAGNGDAIFKRLMSAIGR+DMANDPGLARNDGRV Sbjct: 241 RERTGGALPGIVPSNTYTTGEGMNIVIAGNGDAIFKRLMSAIGREDMANDPGLARNDGRV 300 Query: 301 PRTEEIDAAIQQWCSTQTIDSALATLQAADVPVGKIYSVKDMMNDAQFLARDMFEQHQFA 360 PRTEEIDAAIQQWCSTQTI++ALATLQAADVPVGKIYSVKDMMNDAQFLARDMFEQHQFA Sbjct: 301 PRTEEIDAAIQQWCSTQTIETALATLQAADVPVGKIYSVKDMMNDAQFLARDMFEQHQFA 360 Query: 361 DGTPVKLPGITPKMSETPGQTRWLGPELGAHSDEILQSLGYDEAHIKKLRDAGVV 415 DGTPVKLPGITPKMSETPGQT+WLGPELGAHSDEILQSLGYDEAHIK LRD GVV Sbjct: 361 DGTPVKLPGITPKMSETPGQTKWLGPELGAHSDEILQSLGYDEAHIKMLRDQGVV 415 >gi|300312108|ref|YP_003776200.1| acyl-CoA transferases/carnitine dehydratase [Herbaspirillum seropedicae SmR1] Length = 399 Score = 152 bits (385), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 120/410 (29%), Positives = 195/410 (47%), Gaps = 21/410 (5%) Query: 10 PLAGIKVLELGTLIAGPFCSRMLAEFGAEVIKIEAPGDGDPLRHWRVLKDGTSLWWSVQA 69 PL GI V+ L IA PFC+R LA+ GA VIKIE PG GD R + +G S + Sbjct: 7 PLEGITVISLEHAIAAPFCTRQLADLGARVIKIERPGVGDFARGYDSRVNGLSSHFVWTN 66 Query: 70 RNKKSITLNMKDERAQQIARQLALDADVIIENYRPGVLEKWKLGYEDLKQLNPATIMVRL 129 R+K+S+TLN+KDE ++I QL ADV+++N PG L + L +P I+ + Sbjct: 67 RSKESLTLNLKDEEGRRIIDQLLQQADVLVQNLAPGAAAGLGLSAQALHARHPRLIVCDI 126 Query: 130 SGYGQTGPLRDLPGFGAIGESMGGIRYVSGHPDRPPVRIGISIGDSIAALHGVIGAMMAL 189 SGYG+ GP RD + + +S G V+G PD V+ G SI D A ++ + AL Sbjct: 127 SGYGEDGPYRDKKAYDLLIQSEAGFLSVTGTPD-DVVKAGCSIADIAAGMYAYSNILAAL 185 Query: 190 RHRDVSGGRWNGKSGADCVAGQGQMVDVALYEAVFNMMESMVPEFDFAGVVRE-RTGGAL 248 K G + G+G +D+++ E++ M S + + G R+G Sbjct: 186 I-----------KRGREGREGRGSHIDLSMLESLAEWM-SFPLYYAYQGASAPLRSGAEH 233 Query: 249 PGIVPSNTYTTGEGMNIVIAGNGDAIFKRLMSAIGRD-DMANDPGLARNDGRVPRTEEID 307 I P ++ G+G +++ + +K + +D +A+D + N R + + Sbjct: 234 ATIYPYGPFSAGDGKTVMLGLQNEREWKVFCEQVLQDAALASDARFSSNARRHEHRDVLR 293 Query: 308 AAIQQWCSTQTIDSALATLQAADVPVGKIYSVKDMMNDAQFLARDMFEQHQFADGTPV-K 366 I + + + A L A + ++ +++++ Q AR + + TPV K Sbjct: 294 QLILDVFAGLSAEQVAARLDEAQIANARVNTMQELWQHPQLAARQRWTE----VATPVGK 349 Query: 367 LPGITPKMSETPGQTRWLG-PELGAHSDEILQSLGYDEAHIKKLRDAGVV 415 + + P + R P LG H++ IL +LGY+ I LR+ G V Sbjct: 350 VAALLPPGANDSYDYRMEAIPALGQHTESILSALGYEAGQIAALRERGAV 399 >gi|300310600|ref|YP_003774692.1| acyl-CoA transferases/carnitine dehydratase [Herbaspirillum seropedicae SmR1] Length = 469 Score = 73.6 bits (179), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 50/149 (33%), Positives = 74/149 (49%), Gaps = 12/149 (8%) Query: 6 SKPGPLAGIKVLELGTLIAGPFCSRMLAEFGAEVIKIEAPGDGDPLRHWRVLKDGTSLWW 65 S+ PL GI+VL+L ++AGP +R LA GAEV++I+ G +P V+ Sbjct: 203 SRDRPLRGIRVLDLTRILAGPVATRFLAGLGAEVLRIDPYGWEEPNTVPEVVL------- 255 Query: 66 SVQARNKKSITLNMKDERAQQIARQLALDADVIIENYRPGVLEKWKLGYEDLKQLNPATI 125 K+ LN+KD R+L AD+++ YR L K L ++LNPA I Sbjct: 256 -----GKRCARLNLKDPADLDTLRRLMGHADILVHGYRSDALAKLGLDAAQRRELNPALI 310 Query: 126 MVRLSGYGQTGPLRDLPGFGAIGESMGGI 154 V L YG +G + GF ++ + GI Sbjct: 311 DVSLDAYGWSGAWQCRRGFDSLIQMSTGI 339 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,499,240 Number of extensions: 70301 Number of successful extensions: 196 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 194 Number of HSP's successfully gapped: 3 Length of query: 415 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 327 Effective length of database: 1,203,190 Effective search space: 393443130 Effective search space used: 393443130 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)