BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0174.p (609 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312970|ref|YP_003777062.1| nitrate/sulfonate/bicarbonate A... 620 e-179 gi|300312969|ref|YP_003777061.1| nitrate/sulfonate/bicarbonate A... 560 e-160 gi|300312685|ref|YP_003776777.1| nitrate/sulfonate/bicarbonate A... 293 3e-80 gi|300312686|ref|YP_003776778.1| nitrate/sulfonate/bicarbonate A... 195 1e-50 gi|300309717|ref|YP_003773809.1| nitrate/sulfonate/bicarbonate A... 186 5e-48 >gi|300312970|ref|YP_003777062.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 345 Score = 620 bits (1600), Expect = e-179, Method: Compositional matrix adjust. Identities = 311/345 (90%), Positives = 320/345 (92%), Gaps = 20/345 (5%) Query: 1 MKLSWKQLLIAAGLFLAGYFSFNGSAGA---------------GVFXF-----REQLGYF 40 MKLSWKQLLIAAGLFLAGYFSFNGSAGA +F + EQLGYF Sbjct: 1 MKLSWKQLLIAAGLFLAGYFSFNGSAGAQALEKPKVSIAVGGKNLFYYLPLTIAEQLGYF 60 Query: 41 KDEGLQVEISDFAGGAKALQAMVGGSADVVSGAFEHTINMQAKNQPIVAFVLQGRAPQIV 100 KDEGLQVEISDFAGGAKALQAMVGGSADVVSGA+EHTINMQAKNQPIVAFVLQGRAPQIV Sbjct: 61 KDEGLQVEISDFAGGAKALQAMVGGSADVVSGAYEHTINMQAKNQPIVAFVLQGRAPQIV 120 Query: 101 LAVNNKTMPNYKTVADLKGKKIGVTAPGSSTSIMASYVLAKAGLKPTDVSFIGVGAAAGA 160 LAVNNKTMPNYKTVADLKGKKIGVTAPGSSTS+MASYVLAKAGLKP+DVSFIGVGAAAGA Sbjct: 121 LAVNNKTMPNYKTVADLKGKKIGVTAPGSSTSVMASYVLAKAGLKPSDVSFIGVGAAAGA 180 Query: 161 ISAIRSGQIDAIANLDPVISMLEQNNEIKVVADTRTLKDTNDVFGGPMPAATLYAPAAFV 220 ISA+RSGQIDAIANLDPVISMLEQ+ EIK +ADTRTLKDTNDVFGGPMPAATLYAPAAF+ Sbjct: 181 ISAVRSGQIDAIANLDPVISMLEQSKEIKTIADTRTLKDTNDVFGGPMPAATLYAPAAFI 240 Query: 221 QKYPNTTQALTNAMVRALKWIQKAGPSDIIKAVPESYLLGDRSLYIDAFMKVRQAISPDG 280 QKYPNTTQALTNAMVRALKWIQKAGPSDIIKAVPESYLLGDRSLYIDAFMKVRQAISPDG Sbjct: 241 QKYPNTTQALTNAMVRALKWIQKAGPSDIIKAVPESYLLGDRSLYIDAFMKVRQAISPDG 300 Query: 281 YFPPGGPDTALRTLKAFEPSLADKQIDLSKTFTNSFVQKANAKYK 325 YFPPGGPDTALRTLKAFEPSLADKQIDLSKTFTNSFVQKANAKYK Sbjct: 301 YFPPGGPDTALRTLKAFEPSLADKQIDLSKTFTNSFVQKANAKYK 345 >gi|300312969|ref|YP_003777061.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 269 Score = 560 bits (1442), Expect = e-160, Method: Compositional matrix adjust. Identities = 267/269 (99%), Positives = 268/269 (99%) Query: 341 MTPALQFDNITCTFVSKDDRSQRYTAVANTDLSIAPGEFVSVVGPTGCGKSTLLNVGAGL 400 MTPALQFDNITCTFVSKDDRSQRYTAVANTDL+IAPGEFVSVVGPTGCGKSTLLNVGAGL Sbjct: 1 MTPALQFDNITCTFVSKDDRSQRYTAVANTDLAIAPGEFVSVVGPTGCGKSTLLNVGAGL 60 Query: 401 LQPSSGSVKVFGEELKGINRRAGYMFQAEALMPWRSALQNVIAGLQYRGHEEKAAQELGE 460 LQPSSGSVKVFGEELKGINRRAGYMFQAEALMPWRSALQNVIAGLQYRGHEEKAA ELGE Sbjct: 61 LQPSSGSVKVFGEELKGINRRAGYMFQAEALMPWRSALQNVIAGLQYRGHEEKAAAELGE 120 Query: 461 QWLARVGLQGFGDRYPHQLSGGMRKRVALAQTLILDPDIILMDEPFSALDIQTRQLMENE 520 QWLARVGLQGFGDRYPHQLSGGMRKRVALAQTLILDPDIILMDEPFSALDIQTRQLMENE Sbjct: 121 QWLARVGLQGFGDRYPHQLSGGMRKRVALAQTLILDPDIILMDEPFSALDIQTRQLMENE 180 Query: 521 VLDLWNAKKKAVLFITHDLDEAIAMSDRVIVLAAGPGTHPIGEFRIDLPRPRDVAEIRMH 580 VLDLWNAKKKAVLFITHDLDEAIAMSDRVIVLAAGPGTHPIGEFRIDLPRPRDVAEIRMH Sbjct: 181 VLDLWNAKKKAVLFITHDLDEAIAMSDRVIVLAAGPGTHPIGEFRIDLPRPRDVAEIRMH 240 Query: 581 PRFVELHQQIWDVLRDEVLKGYQQQKRAA 609 PRFVELHQQIWDVLRDEVLKGYQQQKRAA Sbjct: 241 PRFVELHQQIWDVLRDEVLKGYQQQKRAA 269 >gi|300312685|ref|YP_003776777.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 275 Score = 293 bits (749), Expect = 3e-80, Method: Compositional matrix adjust. Identities = 142/278 (51%), Positives = 199/278 (71%), Gaps = 7/278 (2%) Query: 321 NAKYKXSFVSSNRAATARTTMTPALQFDNITCTFVSKDDRSQRYTAVANTDLSIAPGEFV 380 NA + +F + +AA TPA++ N++C F++ D R+ A+ + +S+A GEFV Sbjct: 2 NAINQHAFTPAGQAAQE----TPAIELRNVSCRFITADGRAT--VALRDFSMSVARGEFV 55 Query: 381 SVVGPTGCGKSTLLNVGAGLLQPSSGSVKVFGEELKGINRRAGYMFQAEALMPWRSALQN 440 +VVGPTGCGKST L++ GLL+P+ G V+V G+ + GI+ R G++FQ +A+ PWRS +N Sbjct: 56 AVVGPTGCGKSTTLSLITGLLKPTLGEVRVMGQPVNGIDPRIGFVFQNDAVFPWRSVREN 115 Query: 441 VIAGLQYRGHEEKAAQELGEQWLARVGLQGFGDRYPHQLSGGMRKRVALAQTLILDPDII 500 V AG +RG + A L ++W+ RVGL FGD YPHQLSGGMRKRVALAQT I P+I+ Sbjct: 116 VAAGPLFRGQPKAQAHALADEWIRRVGLDKFGDHYPHQLSGGMRKRVALAQTFINSPEIL 175 Query: 501 LMDEPFSALDIQTRQLMENEVLDLWNAKKKAVLFITHDLDEAIAMSDRVIVLAAGPGTHP 560 LMDEPFSALD+QTR LM++E+L LW+A +V+F+THDL+EAIA++D+V VL A P T Sbjct: 176 LMDEPFSALDMQTRTLMQDELLRLWSAHSGSVVFVTHDLEEAIALADKVYVLTARPATLK 235 Query: 561 IGEFRIDLPRPRDVAEIRMHPRFVELHQQIWDVLRDEV 598 + IDLPRPR + E+R RF+++ ++IW LR+EV Sbjct: 236 -SVYPIDLPRPRVMEEVRYEQRFIDISRRIWADLREEV 272 >gi|300312686|ref|YP_003776778.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 337 Score = 195 bits (495), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 102/283 (36%), Positives = 159/283 (56%) Query: 35 EQLGYFKDEGLQVEISDFAGGAKALQAMVGGSADVVSGAFEHTINMQAKNQPIVAFVLQG 94 EQLGYFK+EGL VE+ G A ++ G+ V G ++H+I++Q K + I A V+ G Sbjct: 51 EQLGYFKEEGLDVELQSQPAGVDAENELLAGAVQAVVGFYDHSIDLQTKGKEITAIVILG 110 Query: 95 RAPQIVLAVNNKTMPNYKTVADLKGKKIGVTAPGSSTSIMASYVLAKAGLKPTDVSFIGV 154 + P V V+ K P+++++AD KGK +GVT GSST+ + Y+ ++ G+ S + V Sbjct: 111 QVPGEVELVSTKAAPHFQSMADAKGKTLGVTGLGSSTNFLTQYLASRHGITSKQFSVLPV 170 Query: 155 GAAAGAISAIRSGQIDAIANLDPVISMLEQNNEIKVVADTRTLKDTNDVFGGPMPAATLY 214 GA I+A++ G+IDA +P S L + + +V+ D RT++ T GG PA+++Y Sbjct: 171 GADNTFIAAMKQGRIDAGMTTEPTASQLLKTGDAQVLVDLRTVEGTVKALGGLYPASSVY 230 Query: 215 APAAFVQKYPNTTQALTNAMVRALKWIQKAGPSDIIKAVPESYLLGDRSLYIDAFMKVRQ 274 +++ + Q L A V+ L++IQ I +P+ Y ++ LYI A Sbjct: 231 VQRSWLNSHKEDAQKLARAFVKTLRFIQTHTAEQIADKMPKDYYGNNKPLYIQALTNSLP 290 Query: 275 AISPDGYFPPGGPDTALRTLKAFEPSLADKQIDLSKTFTNSFV 317 S DG P GGP T L L F P++ K +DLSKT+T+ FV Sbjct: 291 MYSQDGKMPKGGPQTVLAVLSGFNPNVKGKHVDLSKTYTDEFV 333 >gi|300309717|ref|YP_003773809.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 269 Score = 186 bits (472), Expect = 5e-48, Method: Compositional matrix adjust. Identities = 107/276 (38%), Positives = 166/276 (60%), Gaps = 16/276 (5%) Query: 338 RTTMTPALQFDNITCTFVSKDDRSQRYTAVANTDLSIAPGEFVSVVGPTGCGKSTLLNVG 397 +T +P ++ + F DDR A+ + +L I G+FV ++GP+GCGKSTLLN Sbjct: 3 QTNASPRIRIQGVHKVF-KTDDRE--VVALKDINLDIPDGQFVCLLGPSGCGKSTLLNAI 59 Query: 398 AGLLQPSSGSV----KVFGEELKGINRRAGYMFQAEALMPWRSALQNVIAGLQYRGHEEK 453 AG PSSG + KV E G +R G +FQ AL PW + QNV GL+ +G + Sbjct: 60 AGFALPSSGQILTDDKVVSE--PGPDR--GMVFQEYALFPWMTVEQNVAFGLEIKGTPKA 115 Query: 454 AAQELGEQWLARVGLQGFGDRYPHQLSGGMRKRVALAQTLILDPDIILMDEPFSALDIQT 513 + Q L ++GL F R+P LSGGMR+RVA+A+ L LD I+LMDEPF ALD T Sbjct: 116 EIHQRVTQLLDKLGLIDFRTRFPKDLSGGMRQRVAIARVLALDSPIMLMDEPFGALDALT 175 Query: 514 RQLMENEVLDLWNAKKKAVLFITHDLDEAIAMSDRVIVLAAGPGTHPIGEFRIDLPRPRD 573 R+ +++E+L +W+ +K ++F+TH ++EAI ++DR++V+ PGT + ++LPR RD Sbjct: 176 RRNLQDELLRIWDEFRKTIVFVTHSIEEAIYLADRIVVMTYRPGTVK-RDMLVNLPRLRD 234 Query: 574 VAEIRMHPRFVELHQQIWDVLRDEVLKGYQQQKRAA 609 A+ P F L +++ ++ +E + + + +AA Sbjct: 235 PAD----PEFNALKRELGQLVMEEQQRHHNAEMKAA 266 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,822,020 Number of extensions: 76046 Number of successful extensions: 648 Number of sequences better than 1.0e-08: 130 Number of HSP's gapped: 482 Number of HSP's successfully gapped: 139 Length of query: 609 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 518 Effective length of database: 1,188,985 Effective search space: 615894230 Effective search space used: 615894230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)