BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0169.p (488 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 632 0.0 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 486 e-138 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 355 6e-99 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 339 3e-94 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 286 3e-78 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 632 bits (1630), Expect = 0.0, Method: Compositional matrix adjust. Identities = 302/485 (62%), Positives = 371/485 (76%), Gaps = 4/485 (0%) Query: 1 MLSLHDPDLFRMQCPIGGHWQDALDGTTIAVTDPATGLILGTVPAMGTAETRAAVQAAEA 60 ML L DP L R Q + G W DA G +AV +PA G +LG VP MG AETR A++AA A Sbjct: 1 MLQLKDPALLRQQAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANA 60 Query: 61 ALPGWRARTAKERATLLRRWHALLIENQEDLALLMTAEQGKPLSEASGEIAFAASFIEWF 120 A P W+ +TAKER+ +LRRW+ L++ N +DLAL+MTAEQGKPL+EA GEI +AASFIEWF Sbjct: 61 AWPAWKKKTAKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWF 120 Query: 121 AEEARRINGDVIASPSNDRRLLTLRQPVGVCAAITPWNFPAAMITRKAAPALAAGCTMVI 180 AEE +R GD I SPS R++ +++ +GVCAAITPWNFPAAMITRKA PALAAGC MV+ Sbjct: 121 AEEGKRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVL 180 Query: 181 RPASQTPYSALAMVALAERAGIPPGVLNVVTGHPEAIGAELTGNPVVRKLSFTGSTAVGA 240 +PA TP+SALA+ LAERAGIP GV +VVTG P+ IG E+T NP+VRK+SFTGST VG Sbjct: 181 KPAEATPFSALALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGK 240 Query: 241 RLMAQGAGHIQKLSLELGGNAPFIVFDDADLDAAIEGAMISKYRNGGQTCVCSNRMYVQS 300 LM Q A I+KLSLELGGNAPFIVFDDADLDAA+EGA+ SKYRN GQTCVC+NR+YVQ Sbjct: 241 LLMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQD 300 Query: 301 GLYDAFVERLASRASKLVLGDGRAPRTQIGPLIDARALEKISELVEDARSQGARIVCGGT 360 G+YDAF +L K +G G GPLI+ +A++K+ + V DA ++GAR++ GG Sbjct: 301 GVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGK 360 Query: 361 PVVLEGDEQARYFAPTVLTGVKPSMRVAREEIFGPVAPVLRFESEEEVIGQANDTEYGLA 420 L +F PTVL V P+M+VAREE FGP+AP+ RF+++EEV+ ANDTE+GLA Sbjct: 361 RHAL----GHSFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLA 416 Query: 421 SYVYTRDMARVWRVAEALEFGMVGVNTGLISTAEAPFGGVKQSGLGREGSKYGIEEYLEL 480 SY Y+RD+ R+WRVAE LE GMVG+NTGLIS APFGGVKQSGLGREGS YGI++YL + Sbjct: 417 SYFYSRDIGRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVV 476 Query: 481 KYLCL 485 KYLC+ Sbjct: 477 KYLCM 481 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 486 bits (1251), Expect = e-138, Method: Compositional matrix adjust. Identities = 266/487 (54%), Positives = 352/487 (72%), Gaps = 5/487 (1%) Query: 2 LSLHDPDLFRMQCPIGGHWQDALDGTTIAVTDPATGLILGTVPAMGTAETRAAVQAAEAA 61 L+L DL IG W+ A DG + V+DPATG + +VP G A+ RAAV+AA AA Sbjct: 4 LTLQRQDLMPGAQLIGADWRGAADGRQLDVSDPATGQVFASVPDGGAADARAAVEAAVAA 63 Query: 62 LPGWRARTAKERATLLRRWHALLIENQEDLALLMTAEQGKPLSEASGEIAFAASFIEWFA 121 WRA AK+RA +++RW+ LL+ +Q+DL L++ EQGKPL+EA GE+A+AAS++EWF Sbjct: 64 FAAWRATPAKQRAGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFG 123 Query: 122 EEARRINGDVIASPSNDRRLLTLRQPVGVCAAITPWNFPAAMITRKAAPALAAGCTMVIR 181 EEA R NGD+I +P RR++ L++PVGV AAITPWNFPAAMI RK APALAAGCT+V + Sbjct: 124 EEATRANGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCK 183 Query: 182 PASQTPYSALAMVALAERAGIPPGVLNVVTGH----PEAIGAELTGNPVVRKLSFTGSTA 237 PA TP ++LA+V LA+ AG+P GV+N+VT PE + L + VRK+SFTGSTA Sbjct: 184 PAEDTPLTSLALVRLAQEAGVPVGVINIVTASRERTPEVVDVWLA-DGRVRKISFTGSTA 242 Query: 238 VGARLMAQGAGHIQKLSLELGGNAPFIVFDDADLDAAIEGAMISKYRNGGQTCVCSNRMY 297 VG L A ++KLSLELGGNAPFIVFDDAD+DAAI+G M +K+RNGGQTCV NR+Y Sbjct: 243 VGKHLARHSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNRIY 302 Query: 298 VQSGLYDAFVERLASRASKLVLGDGRAPRTQIGPLIDARALEKISELVEDARSQGARIVC 357 VQ +YDAFV++L +R + L +G P +QIGP+I+ARA+ KI + V DA ++GAR++ Sbjct: 303 VQEKVYDAFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIARGARVIT 362 Query: 358 GGTPVVLEGDEQARYFAPTVLTGVKPSMRVAREEIFGPVAPVLRFESEEEVIGQANDTEY 417 GG + G Y+APTVL V +M+ + EE FGPVAP+ RF +E+EVI AN T + Sbjct: 363 GGKRLQGPGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANATPF 422 Query: 418 GLASYVYTRDMARVWRVAEALEFGMVGVNTGLISTAEAPFGGVKQSGLGREGSKYGIEEY 477 GLA+Y Y+ D+ R+ RV +ALE G+VGVN G ++ APFGGVK+SG GREGS +G+++Y Sbjct: 423 GLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGLDDY 482 Query: 478 LELKYLC 484 L KY+C Sbjct: 483 LHTKYVC 489 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 355 bits (910), Expect = 6e-99, Method: Compositional matrix adjust. Identities = 189/473 (39%), Positives = 281/473 (59%), Gaps = 4/473 (0%) Query: 12 MQCPIGGHWQDALDGTTIAVTDPATGLILGTVPAMGTAETRAAVQAAEAALPGWRARTAK 71 +Q I G W + G TI V +PAT ++G + A+ A++AA WR +A Sbjct: 5 VQLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAF 64 Query: 72 ERATLLRRWHALLIENQEDLALLMTAEQGKPLSEASGEIAFAASFIEWFAEEARRINGDV 131 ER+ ++RR LL E E++A LMT EQGKPL+EA E AA I+WFAEEARR G + Sbjct: 65 ERSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRL 124 Query: 132 IASPSNDRRLLTLRQPVGVCAAITPWNFPAAMITRKAAPALAAGCTMVIRPASQTPYSAL 191 + + + +++PVG AA TPWNFP + RK + ALAAGC+++++ +TP S Sbjct: 125 VPARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPA 184 Query: 192 AMVALAERAGIPPGVLNVVTGHPEAIGAELTGNPVVRKLSFTGSTAVGARLMAQGAGHIQ 251 ++ AG+P GV+N+V G P I L +PV++K+SFTGST VG +L A H++ Sbjct: 185 ELIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMK 244 Query: 252 KLSLELGGNAPFIVFDDADLDAAIEGAMISKYRNGGQTCVCSNRMYVQSGLYDAFVERLA 311 + ++ELGG+AP +VFDDAD+D A + +K+RN GQ CV R VQ G+++AFV++ Sbjct: 245 RATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFV 304 Query: 312 SRASKLVLGDGRAPRTQIGPLIDARALEKISELVEDARSQGARIVCGGTPVVLEGDEQAR 371 + A +L +G+G +GP+ + R + + L++DA SQGA++ GG + +G Sbjct: 305 TYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKG----Y 360 Query: 372 YFAPTVLTGVKPSMRVAREEIFGPVAPVLRFESEEEVIGQANDTEYGLASYVYTRDMARV 431 +F PTVLT + R EE FGP+A + FE+ +EVI +AN YGLA+Y + + Sbjct: 361 FFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTK 420 Query: 432 WRVAEALEFGMVGVNTGLISTAEAPFGGVKQSGLGREGSKYGIEEYLELKYLC 484 +A +E GM+ +N ++ E PFGG+K SG G EG IE Y+ K + Sbjct: 421 HALAARVESGMLTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLVT 473 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 339 bits (870), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 202/478 (42%), Positives = 285/478 (59%), Gaps = 7/478 (1%) Query: 7 PDLFRMQCPIGGHWQDALDGTTIAVTDPATGLILGTVPAMGTAETRAAVQAAEAALPGWR 66 PDL Q I G + V DPATG +LG +P A+ A+QAA A WR Sbjct: 3 PDL---QLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWR 59 Query: 67 ARTAKERATLLRRWHALLIENQEDLALLMTAEQGKPLSEASGEIAFAASFIEWFAEEARR 126 + ER+ +LRR AL E + +A +T + GKPL++A EI AA ++W AEE RR Sbjct: 60 QVSPLERSDILRRAAALARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRR 119 Query: 127 INGDVIASPSNDRRLLTLRQPVGVCAAITPWNFPAAMITRKAAPALAAGCTMVIRPASQT 186 + G V+ + + R L +R+PVGVCAAI+PWNFP +K A A+AAGCT+V++ S++ Sbjct: 120 LYGRVVGARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSES 179 Query: 187 PYSALAMVALAERAGIPPGVLNVVTGHPEAIGAELTGNPVVRKLSFTGSTAVGARLMAQG 246 P + + M L AG+P GV N+V+G I L +P+VR +SFTGS VG++L AQ Sbjct: 180 PAAIVLMAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQA 239 Query: 247 AGHIQKLSLELGGNAPFIVFDDADLDAAIEGAMISKYRNGGQTCVCSNRMYVQSGLYDAF 306 A H++++S+ELGG+AP +VFDDA+++ A K RN GQ CV +R YVQ G+Y F Sbjct: 240 ALHMKRMSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARF 299 Query: 307 VERLASRASKLVLGDGRAPRTQIGPLIDARALEKISELVEDARSQGARIVCGGTPVVLEG 366 V A ++ +G G P Q+GPLI R E + L++DA +QG+ +V GG + Sbjct: 300 VTAFAEALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSELVTGGARL---- 355 Query: 367 DEQARYFAPTVLTGVKPSMRVAREEIFGPVAPVLRFESEEEVIGQANDTEYGLASYVYTR 426 ++ ++APT++TGV PS RV +EE FGP+A V F EE I AN GLASYV+T+ Sbjct: 356 GQRGHFYAPTLITGVPPSARVMQEEPFGPIAAVTPFSQAEEAIAMANALPLGLASYVFTQ 415 Query: 427 DMARVWRVAEALEFGMVGVNTGLISTAEAPFGGVKQSGLGREGSKYGIEEYLELKYLC 484 + RV+ L+ GMV +N ++ E PFGG+ +SG G EG G E +L K + Sbjct: 416 SLDTAHRVSRDLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGVEGFESFLVTKMIS 473 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 286 bits (732), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 179/468 (38%), Positives = 252/468 (53%), Gaps = 10/468 (2%) Query: 16 IGGHWQDALDGTTIAVTDPATGLILGTVPAMGTAETRAAVQAAEAALPGWRARTAKERAT 75 I G WQ AV PAT + + A+ AVQAA A+P W A + ERA Sbjct: 20 IDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIPAWAATSPLERAA 79 Query: 76 LLRRWHALLIENQEDLALLMTAEQGKPLSEASGEIAFAASFIEWFAEEARRINGDVIASP 135 LL R HALL+E E A +T E G P+S A + + AE R ++ P Sbjct: 80 LLGRVHALLLERSELFAQALTLEMGAPISYAR------TAHVPLAAEHLRVARDNLADYP 133 Query: 136 SNDRRLLT--LRQPVGVCAAITPWNFPAAMITRKAAPALAAGCTMVIRPASQTPYSALAM 193 R T +R+P+GVCA ITPWN+P IT K PALAAGCT+V++P+ +P SAL Sbjct: 134 FIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSALLF 193 Query: 194 VALAERAGIPPGVLNVVTGHPEAIGAELTGNPVVRKLSFTGSTAVGARLMAQGAGHIQKL 253 + AG+P GV N+V G +GA L+ +P V +S TGST G + A ++++ Sbjct: 194 AEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTVKRV 253 Query: 254 SLELGGNAPFIVFDDADLDAAIEGAMISKYRNGGQTCVCSNRMYVQSGLYDAFVERLASR 313 + ELGG +P +V DADL+ AI + + RN GQ+C R+ V E + Sbjct: 254 AQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELALAT 313 Query: 314 ASKLVLGDGRAPRTQIGPLIDARALEKISELVEDARSQGARIVCGGTPVVLEGDEQARYF 373 +++ +GD T GPL + ++ +++E GAR++ GG P +G Q Y Sbjct: 314 LAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGG-PGRPDGLAQGYYA 372 Query: 374 APTVLTGVKPSMRVAREEIFGPVAPVLRFESEEEVIGQANDTEYGLASYVYTRDMARVWR 433 PT+ + V M +A+EEIFGPV +L +++ EE I ANDT YGL ++V RD +V Sbjct: 373 RPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQVRA 432 Query: 434 VAEALEFGMVGVNTGLISTAEAPFGGVKQSGLGREGSKYGIEEYLELK 481 VA ++ G V +N +APFGG KQSG GRE G+EEY+E+K Sbjct: 433 VAARIQSGQVHLNYPAWDP-QAPFGGYKQSGNGREYGVEGMEEYMEVK 479 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,569,108 Number of extensions: 69580 Number of successful extensions: 316 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 267 Number of HSP's successfully gapped: 21 Length of query: 488 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 399 Effective length of database: 1,198,455 Effective search space: 478183545 Effective search space used: 478183545 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)