BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0165.p (580 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 580 e-167 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 555 e-159 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 361 e-101 gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 208 1e-54 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 196 3e-51 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 580 bits (1496), Expect = e-167, Method: Compositional matrix adjust. Identities = 293/569 (51%), Positives = 389/569 (68%), Gaps = 6/569 (1%) Query: 12 RSQRWLAPDDMRSFAHRQRLQQMGLRREDFMTRPVIAVINTWSDLSPCHAHLRERAEVVK 71 RSQ W D F +R ++ G+ + F RPVI + NT+S+L+PC++H R AE VK Sbjct: 11 RSQAWFGRQDRDGFIYRSWVKNRGIPHDQFDGRPVIGICNTYSELTPCNSHFRALAEQVK 70 Query: 72 RGILMAGGLPVELPAMSLGEVMVKPTTMIYRNMLAMETEELLRSLPIDGAILMGGCDKTT 131 GI AGG P+E P MSLGE +++PT M+YRN+ +M+ EE +R+ P+DG +L+ GCDKTT Sbjct: 71 IGIWEAGGFPLEFPVMSLGETLLRPTAMLYRNLASMDVEESIRANPLDGVVLLMGCDKTT 130 Query: 132 PGLMMGALSADLPCLYVPAGPMLSDRYNGQVVGAGTHTRKFWDEYVAGNIDKEEWIALES 191 P L+MGA S D+P + V GPMLS +Y G+ +G+GT + ++ AG + +EE+ ES Sbjct: 131 PALLMGAASVDVPTIGVSGGPMLSGKYRGRELGSGTGVWQMSEDVRAGKMTQEEFFEAES 190 Query: 192 RMTRAPGTCNTMGTASTMTSIVEAMGFTLPGATSIPAMDAAHPRMCAASGERIVAMVWED 251 M R+ G C TMGTASTM S+VEA+G LP +IPA+DA + +G RIV MV ED Sbjct: 191 CMHRSHGHCMTMGTASTMASMVEALGVGLPHNAAIPAVDARRNVLARNAGRRIVQMVEED 250 Query: 252 LKPSCIFSRSAVDNGIIAYMALGGSTNAAIHTIAIAGRGGVELTLDDFVKVGAGIPVLAD 311 L S I +R A +N I A+GGSTNA IH +AIAGR G++L L D+ +G +P L D Sbjct: 251 LVLSKILTRQAFENAIRVNAAIGGSTNAVIHLLAIAGRIGIKLDLKDWDDIGQQLPCLLD 310 Query: 312 IFPSGTRLMEDFYYAGGLPALLDQLKPHLALDCLTVTGLSLGENLARCAASARTANETAI 371 + PSG LMEDFYYAGGLPA++ QL+ + LT G +L EN A NE I Sbjct: 311 LQPSGKFLMEDFYYAGGLPAVIRQLESVIDKTALTANGKTLWEN----CQDAPNWNEEVI 366 Query: 372 RPLDQPVTEGAALAVLRGNLAPNGAVIKPSAASPHLLKHRGRALVFDSMAEMNASIHDPD 431 R D+P E A +A+L+GNLAP+GAVIKPSAA+P LLKHRGRA+VF++ +++ I D + Sbjct: 367 RSFDKPFKEAAGIAILKGNLAPDGAVIKPSAATPALLKHRGRAVVFENSDDLHKRIDDEN 426 Query: 432 LDVDADTVLVLRGGGPIGAPGMPEWGNLPIPKKLLAAGVRDMLRISDARMSGTHYGTCVL 491 LDVD VLVL+ GP G PGM E GN+P+P K+L G+ DM+R+SDARMSGT YGT VL Sbjct: 427 LDVDETCVLVLKNCGPKGYPGMAEAGNMPLPPKILRKGITDMVRVSDARMSGTAYGTVVL 486 Query: 492 HVSPESAVGGPLALIRTGDIIELDVAAGSINVLLSEQELAQRRGQWQAPPLP--RQRGYI 549 HVSPE+A GGPLA+++ GD IELDV A +++ +S++ELA+RR QWQ P LP QRG++ Sbjct: 487 HVSPEAAAGGPLAVVQNGDFIELDVEARKLHLDVSDEELARRRAQWQKPELPPQMQRGWV 546 Query: 550 RLYVEHVSQADKGCDFDFLTGFETPVEPS 578 +LYV+HV QA++G D DFL G P P Sbjct: 547 KLYVDHVQQANQGADLDFLVGKSGPYVPK 575 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 555 bits (1431), Expect = e-159, Method: Compositional matrix adjust. Identities = 283/579 (48%), Positives = 387/579 (66%), Gaps = 17/579 (2%) Query: 2 QEKRKQLADLRSQRWLAPDDMRSFAHRQRLQQMGLRREDFMTRPVIAVINTWSDLSPCHA 61 +K+ + LRS W D F +R ++ G+ +F +PVI + NTWS+L+PC+A Sbjct: 3 SDKKDKSRTLRSAGWFGTADKNGFMYRSWMKNQGIPDHEFQGKPVIGICNTWSELTPCNA 62 Query: 62 HLRERAEVVKRGILMAGGLPVELPAMSLGEVMVKPTTMIYRNMLAMETEELLRSLPIDGA 121 H R+ AE V+RGI+ AGG PVE P S GE ++PT M+ RN+ +M+ EE +R PID Sbjct: 63 HFRKIAEHVRRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEESIRGNPIDAV 122 Query: 122 ILMGGCDKTTPGLMMGALSADLPCLYVPAGPMLSDRYNGQVVGAGTHTRKFWDEYVAGNI 181 +L+ GCDKTTP L+MGA S D+P + V GPML+ ++ G+ +G+GT + ++ AG I Sbjct: 123 VLLTGCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHQGRDIGSGTVVWQLSEQVKAGEI 182 Query: 182 DKEEWIALESRMTRAPGTCNTMGTASTMTSIVEAMGFTLPGATSIPAMDAAHPRMCAASG 241 +++A E+ M+R+ GTCNTMGTASTM + E++G +LP +IPA+DA + SG Sbjct: 183 TIHDFMAAEAGMSRSAGTCNTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHLSG 242 Query: 242 ERIVAMVWEDLKPSCIFSRSAVDNGIIAYMALGGSTNAAIHTIAIAGRGGVELTLDDFVK 301 RIV MVWE L S I +R A +N I A+GGSTNA IH AIAGR GV+L L+D+ + Sbjct: 243 MRIVDMVWEGLTLSKILTRKAFENAIRTNAAIGGSTNAVIHLKAIAGRIGVDLELEDWTR 302 Query: 302 VGAGIPVLADIFPSGTRLMEDFYYAGGLPALLDQLK-----PHLALDCLTVTGLSLGENL 356 +G G P + D+ PSG LME+FYYAGGLPA+L +L PH D LTV G ++ +N+ Sbjct: 303 IGRGTPTIVDLQPSGRYLMEEFYYAGGLPAVLRRLGEADLLPH--KDALTVNGQTMWDNV 360 Query: 357 ARCAASARTANETAIRPLDQPVTEGAALAVLRGNLAPNGAVIKPSAASPHLLKHRGRALV 416 A N+ +RPL +P+ E + +LRGNLAP GAV+KPSAA+P L+KHRGRA+V Sbjct: 361 ----KDAPIYNDEVVRPLAKPLIEDGGICILRGNLAPRGAVLKPSAATPELMKHRGRAVV 416 Query: 417 FDSMAEMNASIHDPDLDVDADTVLVLRGGGPIGAPGMPEWGNLPIPKKLLAAGVRDMLRI 476 F+ I+DPDLDVDA VLV++ GP G PGM E GN+ +P K+LA GV+DM+RI Sbjct: 417 FEDFNHYKERINDPDLDVDASCVLVMKNVGPKGYPGMAEVGNMGLPPKVLATGVKDMVRI 476 Query: 477 SDARMSGTHYGTCVLHVSPESAVGGPLALIRTGDIIELDVAAGSINVLLSEQELAQRRGQ 536 SDARMSGT YGT +LHV+PE+A GGPL +++ GD IELD AG + + +SE+E+ +RR + Sbjct: 477 SDARMSGTAYGTVILHVAPEAAAGGPLGIVQDGDFIELDAYAGKLQLDISEEEM-KRRLE 535 Query: 537 WQAPPLPRQR-----GYIRLYVEHVSQADKGCDFDFLTG 570 +A L ++ GY LYV+ V QAD+GCDFDFL G Sbjct: 536 ARAKVLAERKPEMVGGYQSLYVDRVLQADEGCDFDFLVG 574 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 361 bits (926), Expect = e-101, Method: Compositional matrix adjust. Identities = 216/579 (37%), Positives = 327/579 (56%), Gaps = 23/579 (3%) Query: 11 LRSQRWL-APD--DMRSFAHRQRLQQMGLRREDFMT-RPVIAVINTWSDLSPCHAHLRER 66 RSQ W PD DM + + +R G+ E+ + RP+I + + SD+SPC+ E Sbjct: 11 FRSQDWFDNPDHIDMTAL-YLERFMNYGITAEELRSGRPIIGIAQSGSDISPCNRIHLEL 69 Query: 67 AEVVKRGILMAGGLPVELPAMSLGEVMVKPTTMIYRNMLAMETEELLRSLPIDGAILMGG 126 A+ V+ GI AGG+P+E P + E +PT I RN+ + E+L PID +L G Sbjct: 70 AKRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLAYLGLVEILHGYPIDAVVLTTG 129 Query: 127 CDKTTPGLMMGALSADLPCLYVPAGPMLSDRYNGQVVGAGTHTRKFWDEYVAGNIDKEEW 186 CDKTTP +M A + D+P + + GPML +G++VG+G+ K AG+ID E++ Sbjct: 130 CDKTTPSQIMAAATVDIPAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLLSAGSIDNEKF 189 Query: 187 IALESRMTRAPGTCNTMGTASTMTSIVEAMGFTLPGATSIPAMDAAHPRMCAASGERIVA 246 + + + + G CNTMGTASTM ++ EA+G +L G ++IPA +M +G RIV Sbjct: 190 LEIAAASAPSSGHCNTMGTASTMNAMAEALGMSLTGCSAIPAPYRERGQMAYETGRRIVG 249 Query: 247 MVWEDLKPSCIFSRSAVDNGIIAYMALGGSTNAAIHTIAIAGRGGVELTLDDFVKVGAGI 306 M +EDL+PS I +R A + I+ A+GGSTNA H +A+A GVEL +D++K G + Sbjct: 250 MAYEDLRPSAILTRDAFLDAIVVNAAIGGSTNAQPHIMAMARHAGVELQSEDWMKYGYDV 309 Query: 307 PVLADIFPSGTRLMEDFYYAGGLPALLDQLKP--HLALDCLTVTGLSLGENLARCAASAR 364 P+L ++ P+G L E F+ AGG+PA++ +L+ L + +T TG ++ ENL A++ R Sbjct: 310 PLLLNMQPAGKYLGERFHRAGGVPAIMWELQQAGKLRAERITATGKTMAENLQGRASNDR 369 Query: 365 TANETAIRPLDQPVTEGAALAVLRGNLAPNGAVIKPSAASPHLLKH-----------RGR 413 I P P+ E A VL+GNL + A++K S S + R Sbjct: 370 ----EMIYPFAAPLRERAGFLVLKGNLF-DFAIMKTSVISETFRERYLSTPGQENIFECR 424 Query: 414 ALVFDSMAEMNASIHDPDLDVDADTVLVLRGGGPIGAPGMPEWGNLPIPKKLLAAGVRDM 473 A+VFD + +A I+DP L +D +T+L +RG GP+G PG E N+ P L+ GV + Sbjct: 425 AVVFDGSDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRGVSTL 484 Query: 474 LRISDARMSGTHYGTCVLHVSPESAVGGPLALIRTGDIIELDVAAGSINVLLSEQELAQR 533 + D R SGT +L+ SPESAVGG LA +R GD + +D+ G N+L+SE+ELA+R Sbjct: 485 PTLGDGRQSGTSDSPSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLVSEEELARR 544 Query: 534 RGQWQAPPLPRQRGYIRLYVEHVSQADKGCDFDFLTGFE 572 + + P P Q + +Y V Q + G + ++ Sbjct: 545 KSEGIPPVPPSQTPWQEIYRSTVGQLETGACMELALKYQ 583 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 208 bits (529), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 165/598 (27%), Positives = 275/598 (45%), Gaps = 83/598 (13%) Query: 35 GLRREDFMTRPVIAVINTWSDLSPCHAHLRERAEVVKRGILMAGGLPVELPAMSLGEVMV 94 G++ DF T+P+IAV+N+++ P H HL++ ++V R I AGG+ E +++ + Sbjct: 26 GMKDGDF-TKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTIAVDDSGT 84 Query: 95 K-PTTMIY----RNMLAMETEELLRSLPIDGAILMGGCDKTTPGLMMGALSADLPCLYVP 149 M+Y R+++A E ++ + D + + CDK TPG++M A+ ++P ++V Sbjct: 85 HGDARMLYSLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVS 144 Query: 150 AGPMLSDRYNGQVVGAGTHTRK-----FWDEYVAG---NIDKEEWIALESRMTRAPGTCN 201 GPM + G+V+ +K D + NI + +E G+C+ Sbjct: 145 GGPMEA----GKVIKTVNTDKKVIKLDLVDAMIQAGDKNISDADVAEVERSACPTCGSCS 200 Query: 202 TMGTASTMTSIVEAMGFTLPGATSIPAMDAAHPRMCAASGERIVAMVWEDLK-------P 254 M TA++M + E +G +LPG +I A A + +G IV + + P Sbjct: 201 GMFTANSMNCLTEVLGLSLPGNGTIVATHADRKELFLRAGRLIVELAKRHYEQDDYSILP 260 Query: 255 SCIFSRSAVDNGIIAYMALGGSTNAAIHTIAIAGRGGVELTLDDFVKVGAGIPVLADIFP 314 I ++ +N + +++GGSTN +H +A A VE T+ D ++ +P L + P Sbjct: 261 RNICTKETWENAMTLDVSMGGSTNTVLHLLAAAHEAKVEFTMADIDRISRRVPCLCKVAP 320 Query: 315 SGTRL-MEDFYYAGGLPALLDQLKPHLALDCL--TVTGLSLGENL--------------- 356 + +ED + AGG+ ++L +L LD TV +LGE + Sbjct: 321 MTNKYHIEDVHRAGGIISILGELARAGLLDTSRPTVHSKTLGEAIEKFDIRVSSDPAVHK 380 Query: 357 ------------------ARCAASARTANETAIRPLDQPVTEGAALAVLRGNLAPNGAVI 398 R A+ IR + ++ LAVL GN+A G ++ Sbjct: 381 LFRAAPGGVPTQVAFSQEERFEANDLDRANGCIRDREHAYSQDGGLAVLYGNIAEKGCIV 440 Query: 399 KPSAASPHLLKHRGRALVFDSM-AEMNASIHDPDLDVDADTVLVLRGGGPIGAPGMPE-- 455 K + +LK G A VF+S A + + D V A V+++R GP G PGM E Sbjct: 441 KTAGVDESILKFSGTARVFESQDAAVEGILGDK---VKAGDVVIVRYEGPKGGPGMQEML 497 Query: 456 WGNLPIPKKLLAAGVRDMLRISDARMSGTHYGTCVLHVSPESAVGGPLALIRTGDIIELD 515 + I K L + +D R SG G + H SPE+A GG + L+ GD+I++D Sbjct: 498 YPTSYIKSKGLG---KACALFTDGRFSGGSSGLVIGHASPEAAEGGAIGLVEEGDMIDID 554 Query: 516 VAAGSINVLLSEQELAQRR--------GQWQAPPLPRQR---GYIRLYVEHVSQADKG 562 + +IN+ +S +LA+RR WQ P RQR ++ Y + AD+G Sbjct: 555 IPNRTINLRISSDDLAKRRTAMEARGDAAWQ--PGNRQRVVSQALQAYAALTTSADRG 610 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 196 bits (499), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 161/521 (30%), Positives = 240/521 (46%), Gaps = 46/521 (8%) Query: 45 PVIAVINTWSDLSPCHAHLRERAEVVKRGI-------LMAGGLPVELPAMSLGEVMVKPT 97 P +A+++ ++D+ H +++K AGG P ++ G+ ++ Sbjct: 68 PSLAIVSAYNDMLSAHQPFERFPQIIKDAAREAGAVAQFAGGSPAMCDGVTQGQAGME-L 126 Query: 98 TMIYRNMLAMETEELLRSLPIDGAILMGGCDKTTPGLMMGALS-ADLPCLYVPAGPMLSD 156 ++ R+ +AM T L D A+ +G CDK PGL++GAL LP ++VPAGPM S Sbjct: 127 SLFSRDAIAMATAIALSHNMFDAALYLGVCDKIVPGLLIGALHFGHLPAVFVPAGPMTSG 186 Query: 157 RYNGQVVGAGTHTRKFWDEYVAGNIDKEEWIALESRMTRAPGTCNTMGTASTMTSIVEAM 216 N V K Y AG I + E + ES+ GTC GTA++ ++EAM Sbjct: 187 MSNSDKV-------KIRQLYTAGKIGRAELLEAESKSYHGAGTCTFYGTANSNQMLMEAM 239 Query: 217 GFTLPGATSI----PAMDAAHPRMCAASGERIVAMVWEDLKPSCIFSRSAVDNGIIAYMA 272 G LPGA I P DA + +I + + + ++ N I+A A Sbjct: 240 GLHLPGAAFITPNTPLRDALTAAAAKQA-AKITDLGTQYTPVGRMIDEKSIVNAIVALHA 298 Query: 273 LGGSTNAAIHTIAIAGRGGVELTLDDFVKVGAGIPVLADIFPSGTRLMEDFYYAGGLPAL 332 GGSTN +H +AIA G+ + DDF K+ A +P++ I+P+GT + F+ AGG + Sbjct: 299 TGGSTNHTLHLVAIAKAAGIVIDWDDFDKLSAVVPLITKIYPNGTADVNHFHAAGGTGFV 358 Query: 333 LDQLKPH--LALDCLTVTGLSL------------GEN-----LARCAASARTANETAIRP 373 L +L + D T+ G L GEN + A A++ +E + Sbjct: 359 LRELIDAGLMHDDVTTILGQGLRQHCKEPMLAAEGENGRPGIVTWHDAPAQSGDEGVLGT 418 Query: 374 LDQPVTEGAALAVLRGNLAPNGAVIKPSAASPHLLKHRGRALVFDSMAEMNASIHDPDLD 433 + +P L +L+GNL AVIK SA P A+VF S A+ LD Sbjct: 419 VAKPFAADGGLKLLQGNLGR--AVIKISAVKPEHRVVEAPAVVFHSQEAFMAAFKAGQLD 476 Query: 434 VDADTVLVLRGGGPIGAPGMPEWGNLPIPKKLLAAGVRDMLRISDARMSGTHYGT-CVLH 492 D V+ +G A GMPE L +L R + ++D RMSG +H Sbjct: 477 RDFVAVITFQG---PRANGMPELHALTPALGILQDKGRHVALVTDGRMSGASGKVPAAIH 533 Query: 493 VSPESAVGGPLALIRTGDIIELDVAAGSINVLLSEQELAQR 533 V+PE GGPL +R GDII LD AG++ + E A R Sbjct: 534 VTPEVLGGGPLGRVRDGDIIRLDAEAGTLEAKVDATEWAAR 574 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,944,870 Number of extensions: 87086 Number of successful extensions: 293 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 278 Number of HSP's successfully gapped: 6 Length of query: 580 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 490 Effective length of database: 1,193,720 Effective search space: 584922800 Effective search space used: 584922800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)