BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0161.p (273 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310374|ref|YP_003774466.1| dehydrogenase oxidoreductase [H... 209 3e-55 gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herb... 204 6e-54 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 122 4e-29 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 116 2e-27 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 116 2e-27 >gi|300310374|ref|YP_003774466.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 209 bits (531), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 126/266 (47%), Positives = 162/266 (60%), Gaps = 8/266 (3%) Query: 1 MNTSPSTPLPRASYPWLAGQVVFISGGASGIGEALVKAFWEQGARVAFCDIDPAAGQALC 60 M+ +P + AS+P L G+ VFI+GG +GIG A+V+AF +QGA VAF DI A +ALC Sbjct: 1 MSNTPQN-VQLASFPSLKGKRVFITGGGTGIGAAIVEAFAQQGAHVAFVDIATEASEALC 59 Query: 61 QRLAAQPGSAPARH-TPWFAVCDVRDIPAYQVVLDHAAAALGAIRVLVNNAGRDTRHGLD 119 +AA A H P F CD+RDIPA+Q + A LG VLVNNA D RH L+ Sbjct: 60 NEVAA------AGHPKPLFRHCDLRDIPAFQATIAELQAQLGDFDVLVNNAANDQRHKLE 113 Query: 120 ELSVEMWNDMLSVNLSHHVFATQRVAPGMREAGGGAIINLGSISWLRGRPNLIGYTASKA 179 E+++E WND +++N FA Q V GM+ GGG+IIN SISW + YT +KA Sbjct: 114 EVTLEYWNDRIAINQRPSFFAVQSVVEGMKRRGGGSIINFSSISWHQSGGGFPVYTTAKA 173 Query: 180 AISGITRTLARELGEAGIRVNAVLPGAVVTERQTALWRDASSDRQFLDLQCLKFRVEPTH 239 + G+TR LAR+LG IRVN V PG V+TERQ LW D + QCL+ + P H Sbjct: 174 STLGLTRGLARDLGPHKIRVNTVTPGWVMTERQIKLWLDEEGKKAIARNQCLQGDLLPWH 233 Query: 240 IADSVLFLASPQAAAVTGQNLIVDAG 265 +A VLFLA+ +A T Q IVDAG Sbjct: 234 LARMVLFLAADDSAMCTAQEFIVDAG 259 >gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 204 bits (519), Expect = 6e-54, Method: Compositional matrix adjust. Identities = 112/264 (42%), Positives = 150/264 (56%), Gaps = 7/264 (2%) Query: 3 TSPSTP-LPRASYPWLAGQVVFISGGASGIGEALVKAFWEQGARVAFCDIDPAAGQALCQ 61 T+ +TP A Y LAG+ V I+GG SGIG ALV+AF QGA+V F DI QAL Sbjct: 2 TAATTPAFEHALYRSLAGKRVVITGGGSGIGAALVEAFVGQGAQVCFLDIAAEPSQALVA 61 Query: 62 RLAAQPGSAPARHTPWFAVCDVRDIPAYQVVLDHAAAALGAIRVLVNNAGRDTRHGLDEL 121 L P + P F C++ ++ A + +G + +L+NNA D RH ++ Sbjct: 62 SLKDAPIA------PRFFPCNLMNLEALRATFTEIETVMGGVDILINNAANDDRHKTADV 115 Query: 122 SVEMWNDMLSVNLSHHVFATQRVAPGMREAGGGAIINLGSISWLRGRPNLIGYTASKAAI 181 + W++ L+VNL H F Q V PGMRE G I+N GSISW G P+L Y +KA I Sbjct: 116 TPAYWDERLAVNLRHQFFCAQAVLPGMRERKQGVILNFGSISWHLGLPDLTLYMTAKAGI 175 Query: 182 SGITRTLARELGEAGIRVNAVLPGAVVTERQTALWRDASSDRQFLDLQCLKFRVEPTHIA 241 G+T +AR+ G G+RVNA++PGA+ T RQT LW + + L QCL RV+P +A Sbjct: 176 EGMTHGMARDFGRDGVRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPTRVDPHDVA 235 Query: 242 DSVLFLASPQAAAVTGQNLIVDAG 265 LFL+S A TG+ VDAG Sbjct: 236 ALALFLSSDSGAKCTGREYYVDAG 259 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 122 bits (305), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 88/254 (34%), Positives = 127/254 (50%), Gaps = 17/254 (6%) Query: 17 LAGQVVFISGGASGIGEALVKAFWEQGARVAFCDIDPAAGQALCQRLAAQPGSAPARHTP 76 G+VV ++G ASGIGEA + F ++GARV D D AA + L P AR T Sbjct: 12 FEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLP--PERTAARET- 68 Query: 77 WFAVCDVRDIPAYQVVLDHAAAALGAIRVLVNNAGRDTRHGLDELSVEMWNDMLSVNLSH 136 DV + ++D A G + VLV++AG + E+S E W+ + + N++ Sbjct: 69 -----DVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNG 123 Query: 137 HVFATQRVAPGMREAGGGAIINLGSISWLRGRPNLIGYTASKAAISGITRTLARELGEAG 196 + + P + E G I+N+ S+S L NL Y ASK A+ +TR +A + G G Sbjct: 124 VFYGAREALPHL-EKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKG 182 Query: 197 IRVNAVLPGAVVTERQTALWRDASSDRQFLDLQCLKFRV----EPTHIADSVLFLASPQA 252 +R+NAV P TA+ D + D LD + + +P IA + FLASP A Sbjct: 183 VRINAVCPSLT----HTAMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLASPDA 238 Query: 253 AAVTGQNLIVDAGL 266 + V G NL VD GL Sbjct: 239 SFVNGVNLPVDGGL 252 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 116 bits (291), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 84/265 (31%), Positives = 128/265 (48%), Gaps = 7/265 (2%) Query: 3 TSPSTPLPRASYPW-LAGQVVFISGGASGIGEALVKAFWEQGARVAFCDIDPAAGQALCQ 61 T+ +TPLP + L G++ I+G + GIG AL + + GA + D A+ Sbjct: 2 TTKTTPLPAGLQGFSLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVAA 61 Query: 62 RLAAQPGSAPARHTPWFAVCDVRDIPAYQVVLDHAAAALGAIRVLVNNAGRDTRHGLDEL 121 +L A+ HT + D + + + +G I +LVNNAG R+ L+E Sbjct: 62 QLRAEG------HTIHESSFDATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEF 115 Query: 122 SVEMWNDMLSVNLSHHVFATQRVAPGMREAGGGAIINLGSISWLRGRPNLIGYTASKAAI 181 W+D++S NL+ FA Q VA M G G IIN+ S+ GRP + YTA+K A+ Sbjct: 116 KESDWHDLISTNLNSVFFAAQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAV 175 Query: 182 SGITRTLARELGEAGIRVNAVLPGAVVTERQTALWRDASSDRQFLDLQCLKFRVEPTHIA 241 T+ +A + G+ GI+VN + PG TE AL DA + + + + Sbjct: 176 KMFTKGMAIDWGKYGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLV 235 Query: 242 DSVLFLASPQAAAVTGQNLIVDAGL 266 + +FLAS + V G L VD G+ Sbjct: 236 GAAVFLASDASRFVNGHILYVDGGV 260 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 116 bits (290), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 94/258 (36%), Positives = 130/258 (50%), Gaps = 15/258 (5%) Query: 13 SYPWLAGQVVFISGGASGIGEALVKAFWEQGARVAFCDID-PAAGQALCQRLAAQPGSAP 71 S L +V F+ GG+ GIG A+V+ +GA VAF + P QAL + A+ G A Sbjct: 11 STSTLVSKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKGGRAL 70 Query: 72 ARHTPWFAVCDVRDIPAYQVVLDHAAAALGAIRVLVNNAGRDTRHGLDELSVEMWNDMLS 131 A D D A Q + AA G + +LVNNAG LD+ +E + L+ Sbjct: 71 AIQ------ADSADAAALQQAIRLAAETFGRLDILVNNAGVLAMGPLDQFKLEDLDRTLA 124 Query: 132 VNLSHHVFATQRVAPGMREAGGGAIINLGSISWLRGRPNLIG--YTASKAAISGITRTLA 189 VN+ ATQ A M E GG +I++GS + R P G Y SK+A+ G+T+ LA Sbjct: 125 VNVRSVFVATQEAARYMGE--GGRVISIGSTNADR-IPFAGGAVYAMSKSALVGLTKGLA 181 Query: 190 RELGEAGIRVNAVLPGAVVTERQTALWRDASSDRQFLDLQCLKFRVEPTHIADSVLFLAS 249 R+LG GI VN V PG V T+ A A+S L L + IA V +LA Sbjct: 182 RDLGPRGITVNNVQPGPVDTDMNPASGDFAAS---LTGLMALPRYGKAEEIASFVAYLAG 238 Query: 250 PQAAAVTGQNLIVDAGLA 267 P++ +TG NL++D G + Sbjct: 239 PESGYITGANLMIDGGFS 256 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 943,269 Number of extensions: 40946 Number of successful extensions: 292 Number of sequences better than 1.0e-08: 47 Number of HSP's gapped: 202 Number of HSP's successfully gapped: 47 Length of query: 273 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 189 Effective length of database: 1,222,130 Effective search space: 230982570 Effective search space used: 230982570 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)