BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0154.p (283 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310325|ref|YP_003774417.1| metal ion ABC transporter perip... 135 4e-33 gi|300314030|ref|YP_003778122.1| metal ion ABC transporter perip... 127 7e-31 gi|300311120|ref|YP_003775212.1| metal ion ABC transporter perip... 127 1e-30 gi|300310887|ref|YP_003774979.1| metal ion ABC transporter perip... 118 5e-28 gi|300311637|ref|YP_003775729.1| metal ion ABC transporter perip... 96 2e-21 >gi|300310325|ref|YP_003774417.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 265 Score = 135 bits (340), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 87/224 (38%), Positives = 133/224 (59%), Gaps = 2/224 (0%) Query: 33 ELKLGFVPGPYIDEFKLGVQPQLEQKGYKIRYYEFSTGLEANSAVFKGDIDANVMQHSVF 92 ++K+G GP+ E V+ LE+ G +++ EF+ ++ N+A+ GD+DAN QH + Sbjct: 29 QIKMGVTAGPHA-EIMEQVKKLLEKDGVQMKVIEFTDYIQPNAALAAGDLDANSYQHQPY 87 Query: 93 LASYNERNKTDLVGIVQVPTPPMGLYSRKHKSLDNLKKGFTVAVPNDPVNLQRALWILQR 152 L + + V + T PMG+YS+K KSL++LK+G V VPNDP N RAL +LQ Sbjct: 88 LDAQIKDRGYKFVSVGSTITFPMGVYSKKIKSLNDLKQGARVGVPNDPTNGGRALLVLQA 147 Query: 153 LGWIRIRENKPVDVTELDVVENKVGLKLVLLENAQGPRALDDADFAAIQGNFAIFAKLK- 211 G I+++ + + T LD+VEN +K+V L+ AQ PR+LDD D A I GN+A A L Sbjct: 148 KGVIKLKADAGLKATPLDIVENPKKIKIVELDAAQLPRSLDDFDAAVINGNYAESAGLSP 207 Query: 212 LSEAFILEDMKPPYINVVAVKKTKQDETYAQDIVQAYRSEFFQK 255 +A +E PY NV+AV+ +D+ + +V+AY S +K Sbjct: 208 TKDAIAVEASTGPYANVIAVRIADKDKPWVAKLVKAYHSPEVKK 251 >gi|300314030|ref|YP_003778122.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 276 Score = 127 bits (320), Expect = 7e-31, Method: Compositional matrix adjust. Identities = 80/260 (30%), Positives = 145/260 (55%), Gaps = 8/260 (3%) Query: 8 LLRLSQIVL-LAVALVGSAQAAPPVRELKLGFVPGPYIDEFKLGVQP-QLEQKGYKIRYY 65 L RLS +++ + +LV +AQAA + +++G GP+ F+ + + + GYK++ Sbjct: 9 LRRLSTLMVSMLASLVLNAQAAAD-QVIRVGVTSGPHAQIFEQARRVFERDNPGYKVKII 67 Query: 66 EFSTGLEANSAVFKGDIDANVMQHSVFLASYNERNKTDLVGIVQVPTPPMGLYSRKHKSL 125 EF+ ++ N+A+ G++DAN QH FL + + L + PM +YSRK++ L Sbjct: 68 EFNDYIQPNAALDAGELDANSYQHRPFLNAQIKTRGYKLYAEGKTMIGPMAIYSRKYRKL 127 Query: 126 DNLKKGFTVAVPNDPVNLQRALWILQRLGWIRIREN----KPVDVTELDVVENKVGLKLV 181 +++ G + +PNDP N R L +LQ+ G+I++R + T +D+ EN K V Sbjct: 128 EDVPVGARIGIPNDPANESRVLLLLQKHGFIKLRAGIDPLTGTNATPIDITENPKKWKFV 187 Query: 182 LLENAQGPRALDDADFAAIQGNFAIFAKLK-LSEAFILEDMKPPYINVVAVKKTKQDETY 240 ++ AQ PR LDD D +A+ ++A A L ++ ++E PY ++AV++ + + + Sbjct: 188 EIDAAQLPRTLDDLDASAVNADYAAKAGLNPARDSLVVESGDSPYACLIAVREKDRAQPW 247 Query: 241 AQDIVQAYRSEFFQKAIRSE 260 +VQAY+S ++ I +E Sbjct: 248 LGKLVQAYQSPEVKRYIETE 267 >gi|300311120|ref|YP_003775212.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 274 Score = 127 bits (319), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 97/267 (36%), Positives = 150/267 (56%), Gaps = 9/267 (3%) Query: 10 RLSQIVLLAVALVGSAQAAPPVRELKLGFVPGPYIDEFKLGVQPQLEQKGYKIRYYEFST 69 RL+ I L++ALV A +++K+G G F + V+ + G +I+ FS Sbjct: 12 RLAAIGALSLALVPQLGQAQDKQKIKVGISVGSAEAIFDV-VKKVAARDGLEIQTVVFSD 70 Query: 70 GLEANSAVFKGDIDANVMQHSVFLASYNERNKTDLVGIVQVPTPPMGLYSRKHKSLDNLK 129 L+ N+A+ GD+DAN QH +L S +V + T P+G+YSRK +S++ L Sbjct: 71 YLQPNAALAAGDLDANAFQHRPYLESQIAARGYRIVPVGLTITAPLGIYSRKFRSVEQLP 130 Query: 130 KGFTVAVPNDPVNLQRALWILQRLGWIRIR----ENKPVDVTELDVVENKVGLKLVLLEN 185 +G + + NDP N RAL +LQ+ G IR++ EN V+ T LDV+EN LKLV L+ Sbjct: 131 QGAAIGIQNDPSNGNRALLLLQKAGLIRLKPGVGENG-VNATPLDVIENPRKLKLVELDA 189 Query: 186 AQGPRALDDADFAAIQGNFAIFAKLKLSEAFI-LEDMKPPYINVVAVKKTKQDETYAQDI 244 AQ PR+LDD A+I ++A A L L + I +ED + Y N++A + +D + + + Sbjct: 190 AQLPRSLDDLAAASINNDYAFKAGLSLQKDTIAVEDPRGRYANIIATRAEDKDRPWVRKL 249 Query: 245 VQAYRSEFFQKAIRSEPFYDGFTLPDY 271 VQAY SE +K I +E + G +P + Sbjct: 250 VQAYHSEEVRKFIETE--FKGSLVPAF 274 >gi|300310887|ref|YP_003774979.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 118 bits (296), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 75/258 (29%), Positives = 132/258 (51%), Gaps = 5/258 (1%) Query: 16 LLAVALVGSAQAAPPVRELKLGFVPGPYIDEFKLGVQPQLEQKGYKIRYYEFSTGLEANS 75 L A A + + A +L + P P+ E ++P L ++G ++ F+ ++ + Sbjct: 9 LFAGAALAFSVGAHAADKLVIAATPVPHA-EILEHIKPALAKEGIDLQVKVFTDYVQPAA 67 Query: 76 AVFKGDIDANVMQHSVFLASYNERNKTDL-VGIVQVPTPPMGLYSRKHKSLDNLKKGFTV 134 + +D N H +L + + +K D+ V + +V P YS+K+K + ++ G T+ Sbjct: 68 QTNEKQVDGNFFLHQPYLDQFKKSHKNDIEVPVAKVHVEPFAAYSQKYKKIADIPNGATI 127 Query: 135 AVPNDPVNLQRALWILQRLGWIRIRENKPVDVTELDVVENKVGLKLVLLENAQGPRALDD 194 A+PNDP N RAL +L R G I++++N + T+ D+VEN LK LE A PR L+ Sbjct: 128 AIPNDPSNSGRALLLLARNGLIKLKDNTNISATQKDIVENPKKLKFRALEAATLPRVLNQ 187 Query: 195 ADFAAIQGNFAIFAKLK-LSEAFILEDMKPPYINVVAVKKTKQDETYAQDIVQAYRSEFF 253 D A I N+A+ AKL + ++ +ED PY N++ ++ +D + + A S Sbjct: 188 VDVALINTNYALEAKLNPVKDSLFIEDANSPYANLLVAREDNKDSPAIKKLAAALNSPDV 247 Query: 254 QKAIRSEPFYDGFTLPDY 271 +K I + Y G +P + Sbjct: 248 KKFIEEK--YQGAIVPAF 263 >gi|300311637|ref|YP_003775729.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 268 Score = 96.3 bits (238), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 65/201 (32%), Positives = 105/201 (52%), Gaps = 2/201 (0%) Query: 9 LRLSQIVLLAVALVGSAQAAPPVRELKLGFVPGPYIDEFKLGVQPQLEQKGYKIRYYEFS 68 ++ S +V L++ ++ S QAA +L++G VPG Y D + + + +++G ++ EF+ Sbjct: 1 MKFSHLVFLSLTVL-SLQAAQAADKLRIGVVPGAYGDSVAVAAK-EAKRQGIDVQVVEFT 58 Query: 69 TGLEANSAVFKGDIDANVMQHSVFLASYNERNKTDLVGIVQVPTPPMGLYSRKHKSLDNL 128 N A+ GD+D N QH FL + ++N L +GLYS K+KSL L Sbjct: 59 DWTTPNVALDAGDLDLNYFQHQPFLDNAIQKNGYKLASAGTGILSNVGLYSLKYKSLTAL 118 Query: 129 KKGFTVAVPNDPVNLQRALWILQRLGWIRIRENKPVDVTELDVVENKVGLKLVLLENAQG 188 +G V + NDPVN RAL +LQ +G I+++ + D+++N L V +E Q Sbjct: 119 PQGAKVGIANDPVNQGRALLLLQNVGLIKLKPKVGYLGSIDDIIDNPRKLSFVEVEGPQL 178 Query: 189 PRALDDADFAAIQGNFAIFAK 209 R D D A +F + AK Sbjct: 179 VRITPDVDLAQGYPHFIVAAK 199 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 853,067 Number of extensions: 33654 Number of successful extensions: 99 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 87 Number of HSP's successfully gapped: 5 Length of query: 283 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 199 Effective length of database: 1,222,130 Effective search space: 243203870 Effective search space used: 243203870 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)