BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0150.p (453 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylas... 815 0.0 gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylas... 238 7e-64 gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herba... 139 5e-34 >gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 429 Score = 815 bits (2104), Expect = 0.0, Method: Compositional matrix adjust. Identities = 408/426 (95%), Positives = 418/426 (98%) Query: 14 MPDNPQWKFETLSVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSR 73 MPDNPQWKFET+SVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSR Sbjct: 1 MPDNPQWKFETISVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSR 60 Query: 74 IMNPTQDVLEKRLAALEGGIAALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLF 133 IMNPTQDVLEKRLAALEGGI ALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLF Sbjct: 61 IMNPTQDVLEKRLAALEGGIGALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLF 120 Query: 134 AHTLPQFGIQTRFADPRQPESFAPLIDERTKAIFIESIGNPLGNVTDIAAVAEIAHRHGV 193 AHTLPQ+G+QTRFADPRQP SF PLID+RTKAI+IESIGNPLGN+TDIAAVA+IAHRHGV Sbjct: 121 AHTLPQYGVQTRFADPRQPASFEPLIDDRTKAIYIESIGNPLGNITDIAAVADIAHRHGV 180 Query: 194 PLIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKHKERFKR 253 PLIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKHK+RFKR Sbjct: 181 PLIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKHKQRFKR 240 Query: 254 LNEPDVSYHGVVYTEALGEAAYIGRARVVPLRNTGAALSPFNAFQILQGIETLSLRLDRI 313 LNEPDVSYHGVVYTEALGEAAYIGRARVVPLRN GAALSPFNAFQILQGIETL LRLDRI Sbjct: 241 LNEPDVSYHGVVYTEALGEAAYIGRARVVPLRNMGAALSPFNAFQILQGIETLGLRLDRI 300 Query: 314 TSNALAVAQYLKRHPKVRWVNYAGLEDHPDHALAQKYFKGRASGVLTFGVANGRDGGARF 373 T+N LAVAQYLKRHPKVRWVNYAGLEDHPDHALAQ YFKGRASGVLTFGVANGR+GGARF Sbjct: 301 TANTLAVAQYLKRHPKVRWVNYAGLEDHPDHALAQTYFKGRASGVLTFGVANGREGGARF 360 Query: 374 QDALQLFTRLVNIGDAKSLATHPASTTHRQLSPAELEKAGVTEDTVRLSIGIEHIDDLLA 433 QDALQLFTRLVNIGDAKSLATHPASTTHRQLSP ELEKAGVTEDTVRLSIGIEHIDDLLA Sbjct: 361 QDALQLFTRLVNIGDAKSLATHPASTTHRQLSPTELEKAGVTEDTVRLSIGIEHIDDLLA 420 Query: 434 DLEQAL 439 DLEQAL Sbjct: 421 DLEQAL 426 >gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 413 Score = 238 bits (607), Expect = 7e-64, Method: Compositional matrix adjust. Identities = 141/429 (32%), Positives = 225/429 (52%), Gaps = 21/429 (4%) Query: 14 MPDNPQWKFETLSVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSR 73 M D + F T +H ++ PI+ +V F ++D + A++F K G Y R Sbjct: 1 MNDKKTYGFTTTILHSDRQKGIEHGSLHKPIHTSVTFGYEDARQLAEVFQGKQPGYRYGR 60 Query: 74 IMNPTQDVLEKRLAALEGGIAALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLF 133 NPT LE ++ +E G + + A+G AA+ +Q + GD++VS++ L+G T +L+ Sbjct: 61 QGNPTVAALEDKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFGNTNSLW 120 Query: 134 AHTLPQFGIQTRFADPRQPESFAPLIDERTKAIFIESIGNPLGNVTDIAAVAEIAHRHGV 193 T+ G + D ++ I T+ +F+E+I NP V D+ + E+ G+ Sbjct: 121 M-TVGAQGAKVSMVDATDVKNVEAAITANTRLVFVETIANPRTQVADLKRIGELCRERGI 179 Query: 194 PLIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKH---KER 250 +VDNT+ +PYL RP GA +VV+SLTK +GGHG LGGA+ D+G+F W ++ E Sbjct: 180 LYVVDNTMTSPYLFRPKTVGAGLVVNSLTKSIGGHGNALGGALTDTGEFDWTRYPHIAEN 239 Query: 251 FKRLNEPDVSYHGVVYTEALGEAAYIGRARVVPLRNTGAALSPFNAFQILQGIETLSLRL 310 +K+ P + + R LR+ G +L P A I G ET++LR Sbjct: 240 YKKNPAPQWG---------------MAQIRAKALRDFGGSLGPEAAHHIAVGAETIALRQ 284 Query: 311 DRITSNALAVAQYLKRHPKVRWVNYAGLEDHPDHALAQKYFKGRASGVLTFGVANGRDGG 370 +R NALA+AQ L+ +V V Y GLE HP HAL++ F+ S +++F + +G D Sbjct: 285 ERECKNALALAQMLQADERVAAVYYPGLESHPQHALSKALFRSFGS-LMSFELKDGID-C 342 Query: 371 ARFQDALQLFTRLVNIGDAKSLATHPASTTHRQLSPAELEKAGVTEDTVRLSIGIEHIDD 430 + + L+L N+GD ++L A T ++ G+ E +R+S+G+E DD Sbjct: 343 FDYLNRLRLAIPTSNLGDTRTLVIPVAHTIFYEMGAERRASMGIAESLIRVSVGLEDTDD 402 Query: 431 LLADLEQAL 439 L+AD QAL Sbjct: 403 LVADFRQAL 411 >gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herbaspirillum seropedicae SmR1] Length = 392 Score = 139 bits (349), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 121/420 (28%), Positives = 183/420 (43%), Gaps = 45/420 (10%) Query: 23 ETLSVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSRIMNPTQDVL 82 +T VH Y A V I+ F D + G Y PT VL Sbjct: 12 QTSLVHSSYRAPDGFEAFPVGIHHASTVLFPDVAAMRSRDWREKLGYTYGLHGTPTSFVL 71 Query: 83 EKRLAALEGGIAALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLFAHTLPQFGI 142 E RLA +EGG SG AA+ ++GD+++ +YG + +L F I Sbjct: 72 EARLAEIEGGKYCRVTPSGLAAIAMVNFAFLKSGDDVLLPDNVYGPSKDLGQWLERDFQI 131 Query: 143 QTRFADPRQPESFAPLIDERTKAIFIESIGNPLGNVTDIAAVAEIAHRHGVPLIVDNTVA 202 RF DP E A LI T+ ++ E+ G+ V DI A+ AH GV + +DNT + Sbjct: 132 TARFYDPMIGEGLAELIRPTTRLVWTEAPGSVSMEVPDIPALCRAAHAKGVLVAIDNTWS 191 Query: 203 TPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKHKERFKRLNEPDVSYH 262 + FEHG DIV+ ++TKY G L GA+ Sbjct: 192 AGIAFKAFEHGVDIVMQAVTKYQSGGSDVLMGAV-------------------------- 225 Query: 263 GVVYTEALGEAAYIGRARVVPLRNTGAALSPFNAFQILQGIETLSLRLDRITSNALAVAQ 322 +V +AL E + R+ G + + + +L+ + ++ LR + + A VA Sbjct: 226 -IVRDQALNEKLELAHMRL------GFGVGMDDVYMVLRSLSSMRLRFEAHDAAARKVAA 278 Query: 323 YLKRHPKVRWVNYAGLEDHPDHALAQKYFKGRASGVLT--FGVANGRDGGARFQDALQLF 380 +LK+ P++ V + ED P HA ++ F G A G+ + F RF D+L+LF Sbjct: 279 WLKQRPEIVAVLHPAFEDCPGHAHWKRDFSG-AGGLFSVIFDPRYVEAQTDRFVDSLRLF 337 Query: 381 TRLVNIGDAKSLATHPASTTHRQLSPAELEKAGVTE-DTVRLSIGIEHIDDLLADLEQAL 439 + G A SL +R + +A + E VR +IG+E +DL+AD+EQAL Sbjct: 338 KIGYSWGGAHSLC-----VPYRMKG---MRRAWMREGQLVRFNIGLESPEDLIADIEQAL 389 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,476,850 Number of extensions: 63532 Number of successful extensions: 190 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 185 Number of HSP's successfully gapped: 3 Length of query: 453 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 365 Effective length of database: 1,203,190 Effective search space: 439164350 Effective search space used: 439164350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)