BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0135.p (305 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312960|ref|YP_003777052.1| catechol 1,2-dioxygenase [Herba... 611 e-176 gi|300310639|ref|YP_003774731.1| catechol 1,2-dioxygenase [Herba... 311 4e-86 gi|300313016|ref|YP_003777108.1| 6-chlorohydroxyquinol-1,2-dioxy... 133 2e-32 gi|300313273|ref|YP_003777365.1| protocatechuate 3,4-dioxygenase... 64 1e-11 >gi|300312960|ref|YP_003777052.1| catechol 1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 303 Score = 611 bits (1576), Expect = e-176, Method: Compositional matrix adjust. Identities = 298/305 (97%), Positives = 301/305 (98%), Gaps = 2/305 (0%) Query: 1 VDKKAIDAVLQKIESTELAEGNQRVKTIVNRLVRDMFYTIEELDVQPEEFWAAVDYLTSA 60 +DKKAIDAVLQKIESTELAEGNQRVKT+VNRLVRDMFYTIEELDVQPEEFWAAVDYLTSA Sbjct: 1 MDKKAIDAVLQKIESTELAEGNQRVKTVVNRLVRDMFYTIEELDVQPEEFWAAVDYLTSA 60 Query: 61 GKSGEFGLIAAGLGFEHFLDLRMDEAEARRGVQGGTPRTIEGPLYVSGAPEQKGFARMDK 120 GKSGEFGLIAAGLGFEHFLDLRMDEAEARRGVQGGTPRTIEGPLYVSGAPEQKGFARMDK Sbjct: 61 GKSGEFGLIAAGLGFEHFLDLRMDEAEARRGVQGGTPRTIEGPLYVSGAPEQKGFARMDK 120 Query: 121 DPQPGDTLFMQGQVFDEHGKPLANALVEVWHANHLGRYSFFDPDQSPFNLRCSIRTDDQG 180 DPQPGDTLFMQGQVFDEHGKPLANALVEVWHANHLGRYSFFDPDQSPFNLRCSIRTD+QG Sbjct: 121 DPQPGDTLFMQGQVFDEHGKPLANALVEVWHANHLGRYSFFDPDQSPFNLRCSIRTDEQG 180 Query: 181 RYRFRSRVPVGYSVPPGGATDRLLNKLGRHGSRPAHIHFFVTVPGYRKLTTQINIAGDPD 240 RYRFRSRVPVGYSVPPGGATDRLL KLGRHGSRPAHIHFFVTVPGYRKLTTQINIAG D Sbjct: 181 RYRFRSRVPVGYSVPPGGATDRLLTKLGRHGSRPAHIHFFVTVPGYRKLTTQINIAG--D 238 Query: 241 PYLWDDFAFATREGLVPELKHVTDPAEIRKHEVDAPFYAIEFNFNLLPEQKDLPKADINR 300 PYLWDDFAFATREGLVPELKHVTDPAEIRKHEVDAPFYAIEFNFNLLPEQ DLPKADINR Sbjct: 239 PYLWDDFAFATREGLVPELKHVTDPAEIRKHEVDAPFYAIEFNFNLLPEQADLPKADINR 298 Query: 301 PRLAA 305 PRLAA Sbjct: 299 PRLAA 303 >gi|300310639|ref|YP_003774731.1| catechol 1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 311 Score = 311 bits (797), Expect = 4e-86, Method: Compositional matrix adjust. Identities = 156/288 (54%), Positives = 197/288 (68%), Gaps = 5/288 (1%) Query: 21 GNQRVKTIVNRLVRDMFYTIEELDVQPEEFWAAVDYLTSAGKSGEFGLIAAGLGFEHFLD 80 GN R K IV+R++ D+F TIE+L++ +E+WA V Y+ G + E GL++ GLG + FLD Sbjct: 26 GNPRTKQIVHRILSDLFKTIEDLEITSDEYWAGVAYINRLGTAHEAGLLSPGLGLDRFLD 85 Query: 81 LRMDEAEARRGVQGGTPRTIEGPLYVSGAPEQKGFARMDKDP-QPGDTLFMQGQVFDEHG 139 +RMD +A G+ GGTPRTIEGPLYV+GAP ++GFAR+D + G T+ M G V+ G Sbjct: 86 MRMDAEDAALGIAGGTPRTIEGPLYVAGAPVEQGFARLDDGSDKSGQTIIMHGTVYGADG 145 Query: 140 KPLANALVEVWHANHLGRYSFFDP--DQSPFNLRCSIRTDDQGRYRFRSRVPVGYSVPPG 197 KP+ A VEVWHAN G YS FDP +Q PFN+R +I TD QGRY+FRS +P Y PP Sbjct: 146 KPMPGAQVEVWHANTKGFYSHFDPTGEQKPFNMRRTIITDAQGRYKFRSIMPTAYGCPPA 205 Query: 198 GATDRLLNKLGRHGSRPAHIHFFVTVPGYRKLTTQINIAGDPDPYLWDDFAFATREGLVP 257 G T LLN LGRHG+RPAHIHFFVT G+RKLTTQINI G DP ++DDFA+ATREGLVP Sbjct: 206 GPTQGLLNALGRHGNRPAHIHFFVTAEGHRKLTTQINIEG--DPLIYDDFAYATREGLVP 263 Query: 258 ELKHVTDPAEIRKHEVDAPFYAIEFNFNLLPEQKDLPKADINRPRLAA 305 L TD A I+ ++ PF I F+ L ++RPRLAA Sbjct: 264 PLVERTDAASIKAEGLEGPFAEIVFDIKLTSLVNGADNQIVDRPRLAA 311 >gi|300313016|ref|YP_003777108.1| 6-chlorohydroxyquinol-1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 296 Score = 133 bits (334), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 89/290 (30%), Positives = 142/290 (48%), Gaps = 13/290 (4%) Query: 7 DAVLQKIESTELAEGNQRVKTIVNRLVRDMFYTIEELDVQPEEFWAAVDYLTSAGK---- 62 D + Q + S +QR++ I+ LV+ + E+ + +E++A +D+LT G+ Sbjct: 15 DTITQAVLSRHAWLTDQRLREIMTSLVQHLHAFAREVKLTEKEWFAGIDFLTETGQICSD 74 Query: 63 -SGEFGLIAAGLGFEHFLDLRMDEAEARRGVQGGTPRTIEGPLYVSGAPEQKGFARMDKD 121 EF L++ LG + + + A G T T+ GP +V AP+ A + Sbjct: 75 QRQEFILLSDTLGLSTLVMAQQNHKPA-----GCTEATVFGPFHVEDAPQYPLGADIANG 129 Query: 122 PQPGDTLFMQGQVFDEHGKPLANALVEVWHANHLGRYSFFDPDQSPFNLRCSIRTDDQGR 181 + G+ F+ G V +G+PLA+A +EVW A+ G Y D + R ++ TD +GR Sbjct: 130 AK-GEPCFVSGSVRGRNGRPLAHARMEVWQADENGFYDVQYADLDQYRARGTLFTDAEGR 188 Query: 182 YRFRSRVPVGYSVPPGGATDRLLNKLGRHGSRPAHIHFFVTVPGYRKLTTQINIAGDPDP 241 Y FRS + Y +P G R+L KLGRH RPAH+HF + GY L T ++ D Sbjct: 189 YHFRSILANPYPIPHDGPVGRMLEKLGRHPWRPAHLHFRIQAEGYETLIT--HVFRRDDV 246 Query: 242 YLWDDFAFATREGLVPELKHVTDPAEIRKHEVDAPFYAIEFNFNLLPEQK 291 YL D F R L+ + + + PFY ++++F L P +K Sbjct: 247 YLDSDAVFGVRSTLIADWPCHPAGTAPDGTQCEQPFYTLDYDFVLNPLRK 296 >gi|300313273|ref|YP_003777365.1| protocatechuate 3,4-dioxygenase beta chain protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 63.9 bits (154), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 47/122 (38%), Positives = 63/122 (51%), Gaps = 19/122 (15%) Query: 125 GDTLFMQGQVFDEHGKPLANALVEVWHANHLGRYSFFDPDQ--SPFNLRCS----IRTDD 178 G+ + + G+V DE GKP+ N+L+EVW N GRY F DQ +P + + + TDD Sbjct: 68 GEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRY-FHKKDQHDAPLDPNFTGFGKMLTDD 126 Query: 179 QGRYRFRSRVPVGYSVPPGGATDRLLNKLGRHGSRPAHIHFFVTVPGY-RKLTTQINIAG 237 GRYRF S P Y P G + RPAHIHF + Y ++L TQ+ Sbjct: 127 NGRYRFVSIKPGPY---PWGNHHK--------AWRPAHIHFSLFGNVYAQRLVTQMYFPN 175 Query: 238 DP 239 DP Sbjct: 176 DP 177 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.140 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,103,084 Number of extensions: 51536 Number of successful extensions: 98 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 90 Number of HSP's successfully gapped: 4 Length of query: 305 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 220 Effective length of database: 1,217,395 Effective search space: 267826900 Effective search space used: 267826900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)