BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0134.p (386 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312959|ref|YP_003777051.1| muconate and chloromuconate cyc... 752 0.0 gi|300310638|ref|YP_003774730.1| muconate cycloisomerase [Herbas... 503 e-144 gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lac... 72 7e-14 gi|300310362|ref|YP_003774454.1| galactonate dehydratase [Herbas... 67 3e-12 >gi|300312959|ref|YP_003777051.1| muconate and chloromuconate cycloisomerase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 752 bits (1942), Expect = 0.0, Method: Compositional matrix adjust. Identities = 370/385 (96%), Positives = 380/385 (98%) Query: 1 LNSTLPATSIETVEVLLLDVPTIRPHRLSMTTMHRQTLVLVRITGNDGVAGWGEATTIGG 60 +N TLPATSIETVEVLLLDVPTIRPHRLSMTTMHRQTLVLVRITGNDGVAGWGEATTIGG Sbjct: 1 MNPTLPATSIETVEVLLLDVPTIRPHRLSMTTMHRQTLVLVRITGNDGVAGWGEATTIGG 60 Query: 61 LAYGEESPESIKVNIDTYITPLLLGADANQVAARMHHIAEHVQGNRFAKCAIETALSDAL 120 LAYGEESPESIKVNIDTYITPLLLGADANQVAARMHHI EHVQGNRFAKCAIETALSDAL Sbjct: 61 LAYGEESPESIKVNIDTYITPLLLGADANQVAARMHHIDEHVQGNRFAKCAIETALSDAL 120 Query: 121 ARRLGVPLSTLFGGRVRESLPIAWTLASGDTARDIEEAEKMLAQRRHNIFKLKIGARSVE 180 ARRLGVPLSTLFGGRVR+SLPIAWTLASGDTARDI+EAEKMLAQRRHN+FKLKIGAR+V+ Sbjct: 121 ARRLGVPLSTLFGGRVRDSLPIAWTLASGDTARDIDEAEKMLAQRRHNLFKLKIGARTVQ 180 Query: 181 QDCAHVAAIKRALGERGSVRVDVNQAWTETEAMLGMQLLADAGVDLVEQPIAATNHDGLG 240 QDCAHVAAIKRALGERGSVRVDVNQAWTETEAMLGMQ LADAGVDLVEQPIAA+NHDGL Sbjct: 181 QDCAHVAAIKRALGERGSVRVDVNQAWTETEAMLGMQWLADAGVDLVEQPIAASNHDGLR 240 Query: 241 RLRQKNRIAIMADESLHGPQDAYQLARVGAADVFAVKINQSGGLAGAQQVAAIAAAAQVE 300 RLRQKNRIAIMADESLHGPQDAYQLAR GAADVFAVKINQSGGLAGAQQVAAIA+AAQVE Sbjct: 241 RLRQKNRIAIMADESLHGPQDAYQLARAGAADVFAVKINQSGGLAGAQQVAAIASAAQVE 300 Query: 301 LYGGTMLEGAIGTMASAQLFATFGTLAWGTELFGPLLLTEEILATPLRYRDFALELPTGP 360 LYGGTMLEGAIGTMASAQLFATFGTLAWG+ELFGPLLLTEEILATPLRYRDFALELPTGP Sbjct: 301 LYGGTMLEGAIGTMASAQLFATFGTLAWGSELFGPLLLTEEILATPLRYRDFALELPTGP 360 Query: 361 GLGITLDEDRVNALRRDRKTPLVSV 385 GLGITLDEDRVNALRRDRKTPLVS+ Sbjct: 361 GLGITLDEDRVNALRRDRKTPLVSL 385 >gi|300310638|ref|YP_003774730.1| muconate cycloisomerase [Herbaspirillum seropedicae SmR1] Length = 398 Score = 503 bits (1295), Expect = e-144, Method: Compositional matrix adjust. Identities = 253/370 (68%), Positives = 296/370 (80%), Gaps = 2/370 (0%) Query: 10 IETVEVLLLDVPTIRPHRLSMTTMHRQTLVLVRITGNDGVAGWGEATTIGGLAYGEESPE 69 I+ VE L+D+PTIRPH+LSM TM+ QTL+LVR+ +DG G GE TTIGGLAYG ESPE Sbjct: 16 IDRVETFLVDLPTIRPHQLSMATMNGQTLMLVRLYCSDGSIGVGEGTTIGGLAYGAESPE 75 Query: 70 SIKVNIDTYITPLLLGADANQVAARMHHIAEHVQGNRFAKCAIETALSDALARRLGVPLS 129 +K+ IDTY PLLLGADA+Q+ A M + + + NRFAKCAIETAL DAL +R G+P+S Sbjct: 76 GMKLAIDTYFAPLLLGADASQIPAIMARLNKAICDNRFAKCAIETALFDALGQRTGLPVS 135 Query: 130 TLFGGRVRESLPIAWTLASGDTARDIEEAEKMLAQRRHNIFKLKIGARSVEQDCAHVAAI 189 L GGR RESLPIAWTLASGDTA+DI+EAE+MLAQRRHNIFKLKIG R V+ D AHVAAI Sbjct: 136 QLLGGRSRESLPIAWTLASGDTAKDIDEAERMLAQRRHNIFKLKIGRREVDADVAHVAAI 195 Query: 190 KRALGERGSVRVDVNQAWTETEAMLGMQLLADAGVDLVEQPIAATNHDGLGRLRQKNRIA 249 K+ALG+RG+VRVDVN AW+E EA G+ L DAG +LVEQP+A+ L RL+ ++ IA Sbjct: 196 KKALGDRGAVRVDVNMAWSELEAQRGLAGLVDAGCELVEQPVASPQ--ALARLKGRHAIA 253 Query: 250 IMADESLHGPQDAYQLARVGAADVFAVKINQSGGLAGAQQVAAIAAAAQVELYGGTMLEG 309 IMADESL GP A+ LAR+G ADVFA+KI QSGGL AQQVAAI AA +ELYGGTMLEG Sbjct: 254 IMADESLTGPASAFALARIGGADVFAIKIEQSGGLRAAQQVAAIGDAAGIELYGGTMLEG 313 Query: 310 AIGTMASAQLFATFGTLAWGTELFGPLLLTEEILATPLRYRDFALELPTGPGLGITLDED 369 AIGT+ASA FATF L WGTELFGPLLLTEEILA PL+Y+DF L +P PGLGITLDE+ Sbjct: 314 AIGTIASAHAFATFRELQWGTELFGPLLLTEEILAEPLQYQDFQLAIPHRPGLGITLDEE 373 Query: 370 RVNALRRDRK 379 R+ RRDRK Sbjct: 374 RLQYFRRDRK 383 >gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lactonizing [Herbaspirillum seropedicae SmR1] Length = 424 Score = 72.0 bits (175), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 75/294 (25%), Positives = 130/294 (44%), Gaps = 34/294 (11%) Query: 108 AKCAIETALSDALARRLGVPLSTLFGGRVRESLPIAWTL---------------ASGDTA 152 A A E A DA AR L VPL L GG VR+ +P + L A G+ Sbjct: 110 AYSAFEVACLDAQARYLNVPLVDLLGGAVRKEIPFSAYLFFKYAQHIDSPYAPDAWGEAL 169 Query: 153 RD---IEEAEKMLAQRRHNIFKLKIGARSVEQDCAHVAAIKRALGERGSVRVDVNQAWTE 209 + + +A KM+ + KLK GA + E + A + A+++A + +R+D N W+ Sbjct: 170 SEEQIVAQARKMIEENGFQSIKLKAGALAPEHEVACIKALRKAFPD-APLRIDPNGNWSL 228 Query: 210 TEAMLGMQLLADAGVDLVEQPIAATNHDGLGRLRQKNRIAIMADESLHGPQDAYQLARVG 269 A+ +LL D + E P +G+ L ++ + + + + D +L R Sbjct: 229 QTAIRMAELLGD-DLQYYEDPTPGL--EGMAELHRRTGLPLATNMVV---TDFDELRRSV 282 Query: 270 AADVFAVKI---NQSGGLAGAQQVAAIA--AAAQVELYGGTMLEGAIGTMASAQLFATFG 324 A D + + + GGL QQ+A + V ++ + L I MA + + A Sbjct: 283 ALDSVQIVLADHHYWGGLRDTQQLAKMCDVFGLGVSMHSNSHL--GISLMAMSHVAAAVE 340 Query: 325 TLAWGTELFGPLLLTEE--ILATPLRYRDFALELPTGPGLGITLDEDRVNALRR 376 L++ + P +E I L R+ +++ PGLG+ +D+D++ L + Sbjct: 341 NLSYACDTHYPWQEPDEEVIQGGKLPIRNGCVQITDAPGLGVEVDQDQLRKLHQ 394 >gi|300310362|ref|YP_003774454.1| galactonate dehydratase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 66.6 bits (161), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 82/355 (23%), Positives = 142/355 (40%), Gaps = 35/355 (9%) Query: 39 VLVRITGNDGVAGWGEATTIGGLAYGEESPESIKVNIDTYITPLLLGADANQVAARMHHI 98 ++I ++GV GWGE E S+ ++ ++ L+G D + H Sbjct: 16 CFLKIETDEGVVGWGEPIV-------EGRAHSVAAAVEE-LSDYLIGKDPRNIE---DHW 64 Query: 99 AEHVQGNRF--------AKCAIETALSDALARRLGVPLSTLFGGRVRESLPIAWTLASGD 150 +G + A I+ AL D + LGVP+ L GG VR S+ + ++ GD Sbjct: 65 TVLYRGGFYRGGAIHMSALAGIDQALWDIKGKDLGVPVHQLLGGAVRNSIRV-YSWIGGD 123 Query: 151 TARDIEEAEKMLAQRRHNIFKLK--------IGARSVEQDCAHVAAIKRALGERGSVRVD 202 D A K R K+ +E A+V A++ A+G + VD Sbjct: 124 RPADTAAAAKSAVARGFTAVKMNGTEELQFIDSHDKIELTLANVQAVREAVGPNVGIGVD 183 Query: 203 VNQAWTETEAMLGMQLLADAGVDLVEQPIAATNHDGLGRLRQKNRIAIMADESLHGPQDA 262 + + A ++ L + +E+P+ + N + L + I E L+ D Sbjct: 184 FHGRVHKPMAKALIKELEPYKLMFIEEPVLSENAEALKEIAHLTSTPIALGERLYSRWDF 243 Query: 263 YQLARVGAADVFAVKINQSGGLAGAQQVAAIAAAAQVELYGGTMLEGAIGTMASAQLFAT 322 ++ G D+ ++ +GG+ +++A +A A V L L G I + Q+ A Sbjct: 244 KRILSEGYVDIIQPDVSHAGGITETRKIAMMAEAYDVALALHCPL-GPIALASCLQVDAG 302 Query: 323 FGTLAWGTELFGPLLLTEEILATPLR------YRDFALELPTGPGLGITLDEDRV 371 + G L +R Y D +++P GPGLGI ++E+ V Sbjct: 303 SYNAFIQEQSLGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQGPGLGIEINEEYV 357 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,159,967 Number of extensions: 47849 Number of successful extensions: 199 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 197 Number of HSP's successfully gapped: 4 Length of query: 386 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 299 Effective length of database: 1,207,925 Effective search space: 361169575 Effective search space used: 361169575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)