BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0111.p (781 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312928|ref|YP_003777020.1| transmembrane acyl-CoA dehydrog... 1271 0.0 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 124 3e-29 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 98 2e-21 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 87 4e-18 >gi|300312928|ref|YP_003777020.1| transmembrane acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 824 Score = 1271 bits (3288), Expect = 0.0, Method: Compositional matrix adjust. Identities = 628/703 (89%), Positives = 659/703 (93%) Query: 1 MIIWLILLIAGALALMMMRASAWVWLVAEAILIWLGARLAEIELPVLVLLAILFFTPTLL 60 MIIWLILLIA ALALMMMRASAW WL AEA+L+WLGARLAEIE PVLVLLAILFFTPTLL Sbjct: 1 MIIWLILLIASALALMMMRASAWAWLAAEAVLLWLGARLAEIEWPVLVLLAILFFTPTLL 60 Query: 61 VAIKSVRQSLMTRPALDLFRKIAPRMSATERDAIEAGTVWWDAELFSGKPDWKRLFELPA 120 VAI+SVRQ+LMTRPALD+FRKI P MSATERDAIEAGTVWWDAELFSGKPDW RLF LPA Sbjct: 61 VAIRSVRQALMTRPALDIFRKIMPAMSATERDAIEAGTVWWDAELFSGKPDWSRLFALPA 120 Query: 121 PRLTPEEQSFLDNEVEQLCAMVSDWDTSVKTQDLPPDAWQFIKDKGFLGMIIPRQYGGKQ 180 PRLT EEQ+FLDNEVEQLC MVSDWD +VKTQDLPP+AWQFIKDKGFLGMIIPRQYGGKQ Sbjct: 121 PRLTAEEQAFLDNEVEQLCGMVSDWDATVKTQDLPPEAWQFIKDKGFLGMIIPRQYGGKQ 180 Query: 181 FSAYMHSQVVMKLGSRSSAAAIAVMVPNSLGPAELLLHYGTDAQKNHYLPRLAQGLEIPC 240 FSAYMHSQVVMKL SRSSAAAIAVMVPNSLGPAELLLHYGT+ QKNHYLPRLAQGLEIPC Sbjct: 181 FSAYMHSQVVMKLASRSSAAAIAVMVPNSLGPAELLLHYGTEVQKNHYLPRLAQGLEIPC 240 Query: 241 FALTSPYAGSDAAAIPDVGIVCKGEHEGRETLGFRVTWSKRYITLAPIATVLGLAFRVRD 300 FALTSPYAGSDAAAIPD+GIVCKG HEGRETLGFRVTWSKRYITLAPIATVLGLAFR D Sbjct: 241 FALTSPYAGSDAAAIPDIGIVCKGVHEGRETLGFRVTWSKRYITLAPIATVLGLAFRASD 300 Query: 301 PDGLLGSDAEPGITCALIPTGHPGVITGRRHWPLNAVFQNGPTEGRDVFIPMEWVIGGAG 360 PDGLLG + E GITCALIPT HPGV+TGRRHWPLNAVFQNGPTEG+DVFIPMEWVIGG Sbjct: 301 PDGLLGGEREAGITCALIPTDHPGVVTGRRHWPLNAVFQNGPTEGKDVFIPMEWVIGGVA 360 Query: 361 QVGRGWRMLMECLAAGRAISLPSSSVGFSKLAVRGTSAYTAMRRQFKIEIGKFEGVQEAL 420 QVGRGWRMLMECLAAGRAISLPSSSVGFSK+AVRGTS Y AMRRQFKIEIGKFEGVQEAL Sbjct: 361 QVGRGWRMLMECLAAGRAISLPSSSVGFSKMAVRGTSGYAAMRRQFKIEIGKFEGVQEAL 420 Query: 421 ARMGGHLYMMDATRRLSALAVDAGEKPSVISAISKYHVTERGRMVVNDGMDVIGGKGICM 480 ARMGGHLYMMDATRRLSALAVDAGEKPSVISAISKYH+TERGRMVVNDGMDVIGGKGICM Sbjct: 421 ARMGGHLYMMDATRRLSALAVDAGEKPSVISAISKYHITERGRMVVNDGMDVIGGKGICM 480 Query: 481 GPGNFLARAYQQVPIAITVEGANILTRCLIIFGQGAIRCHPYVLQELATAADEDRERAVQ 540 GPGNFLAR YQQ+PIAITVEGANILTRCLIIFGQGAIRCHPYVL+ELA AADEDRERAVQ Sbjct: 481 GPGNFLARVYQQIPIAITVEGANILTRCLIIFGQGAIRCHPYVLKELAAAADEDRERAVQ 540 Query: 541 EFDRLLFGHLSFVAANAARSFVGGISRGWLLPSSSHAARATRGYFRAVVHLSSAFALLTD 600 EFDRLLFGHLSFVAANAARS VGG+S GWLL + + AA ATR Y+RAVVHLSSAFALLTD Sbjct: 541 EFDRLLFGHLSFVAANAARSLVGGLSGGWLLAAPAQAAHATRRYYRAVVHLSSAFALLTD 600 Query: 601 VSMGVLGGTLKLRERISARLGDVVSQLYLVSAVLKRFEDEARQEEDLPYVDWSVQDALHR 660 SM VLGGTLK RERISARLGDV+SQLYLVSA LKR+EDE RQE DLPYVDWSVQ+AL+R Sbjct: 601 ASMAVLGGTLKFRERISARLGDVLSQLYLVSAALKRYEDEGRQEADLPYVDWSVQEALNR 660 Query: 661 AQEAIIDVLHNFPNPFIAVLLRVLIFPLGLPYRKPSDVLGXPI 703 AQEAI++VLHNFPNPF+A LR+ IFPLGLPYRKP+DVLG + Sbjct: 661 AQEAIVEVLHNFPNPFVAFALRLAIFPLGLPYRKPADVLGTHV 703 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 124 bits (311), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 111/397 (27%), Positives = 181/397 (45%), Gaps = 37/397 (9%) Query: 123 LTPEEQSFLDN----EVEQLCAMVSDWDTSVKTQDLPPDAWQFIKDKGFLGMIIPRQYGG 178 L+PE + D E+L +DWD + P +A + + + G +GM +P Q+GG Sbjct: 3 LSPEHEMIRDAMRHFAQERLLPFAADWD---RNHTFPAEALKELAELGAMGMCVPEQWGG 59 Query: 179 KQFSAYMHSQVVMKLGSRSSAAAIAVMVPNSLGPAELLLHYGTDAQKNHYLPRLAQGLEI 238 + ++ + A + V V NSL + YG+ QK +L LA+G + Sbjct: 60 AGMDYMSLVLALEEIAAGDGATSTIVSVQNSLACG-ITEKYGSPTQKEQWLKPLARGEML 118 Query: 239 PCFALTSPYAGSDAAAIPDVGIVCKGEHEGRETLGFRVTWSKRYITLAPIATVLGLAFRV 298 CF LT P+ GSDAAAI + E +G + F + +K++IT A V + F V Sbjct: 119 GCFCLTEPHTGSDAAAIR-----TRAERDGDD---FILNGTKQFITSGKHAGV-AIVFAV 169 Query: 299 RDPDGLLGSDAEPGITCALIPTGHPGVITGRRHWPLNAVFQNGPTEGRD-VFIPMEWVIG 357 D S + GI+C L+P PG + GR + + D +P ++ Sbjct: 170 TDR-----SAGKKGISCFLVPCDTPGFVVGRSEEKMGQHASDTVQIMLDNCRVPATALL- 223 Query: 358 GAGQVGRGWRMLMECLAAGRAISLPSSSVGFSKLAVRGTSAYTAMRRQFKIEIGKFEGVQ 417 G+ G G+R+ + L AGR I + + SVG ++ A +Y R F + I + + V Sbjct: 224 --GKEGEGYRIALSNLEAGR-IGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVN 280 Query: 418 EALARMGGHLYMMDATRRL---SALAVDAGEKPSVISAISKYHVTERGRMVVNDGMDVIG 474 LA M ++DA R + +A D G ++++K +E + +D + + G Sbjct: 281 FRLADMN---TLLDAARLMVWRAAQLKDQGRPCLKEASMAKMFASEAAEKIASDAIQIHG 337 Query: 475 GKGICMGPGNF-LARAYQQVPIAITVEGANILTRCLI 510 G G +F + R Y+ V I EGAN + R +I Sbjct: 338 GVGY---TSDFPVERIYRDVRICQIYEGANDIQRLVI 371 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 98.2 bits (243), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 105/400 (26%), Positives = 180/400 (45%), Gaps = 35/400 (8%) Query: 116 FELPAPRLTPEEQSFLDNEVEQLCAMVSDWDTSVKTQDLPPDAWQFIKDKGFLGMIIPRQ 175 FEL +L + QS D ++ + WD P D + GF G+ P Sbjct: 3 FELNQDQLAFQ-QSARDFAAGEMAPFAAKWDEEAH---FPLDVIAKAGELGFCGLYTPEA 58 Query: 176 YGGKQFSAYMHSQVVMKLGSRSSAAAIAVMVPNSLGPAELLLHYGTDAQKNHYLPRLAQG 235 GG S + V +L ++ A + + N + + ++ +G + K + +LA G Sbjct: 59 DGGLGLSRLDATVVFEELARACTSTAAYLTIHNMV--SWMIASWGQPSLKAQWCEQLAAG 116 Query: 236 LEIPCFALTSPYAGSDAAAIPDVGIVCKGEHEGRETLGFRVTWSKRYITLAPIATVLGLA 295 +I + LT P +GSDAA++ + G + + SK +I+ A VL + Sbjct: 117 RKIGSYCLTEPGSGSDAASLKTTARLEDGH--------YVLNGSKAFISGAGSTDVLVVM 168 Query: 296 FRVRDPDGLLGSDAEPGITCALIPTGHPGVITGRRHWPLNAVFQNGPTEG---RDVFIPM 352 R G + G++ ++P PGV G++ + + + PT +V +P Sbjct: 169 ART-------GGEGARGVSAIVVPGNAPGVSYGKKESKMG--WNSQPTRTISFDNVKVPA 219 Query: 353 EWVIGGAGQVGRGWRMLMECLAAGRAISLPSSSVGFSKLAVRGTSAYTAMRRQFKIEIGK 412 + ++G Q G+G+ M+ L GR I++ + SVG ++ A+ AY R+QF I Sbjct: 220 DHLLG---QEGQGFIFAMKGLDGGR-INIATCSVGAAQAALDAAHAYMKERKQFGRPIAD 275 Query: 413 FEGVQEALARMGGHLYMMDATRRLSALAVDAGE-KPSVISAISKYHVTERGRMVVNDGMD 471 F+ +Q LA M L RL+A +DAG + + A++K T+ G V N+ + Sbjct: 276 FQALQFKLADMQTELVAARQMVRLAATKLDAGSPEATTYCAMAKRLATDLGFRVCNEALQ 335 Query: 472 VIGGKG-ICMGPGNFLARAYQQVPIAITVEGANILTRCLI 510 + GG G I P L R ++ V + +EG N + R +I Sbjct: 336 LHGGYGYIREYP---LERYFRDVRVHQILEGTNEIMRVII 372 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 87.4 bits (215), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 89/338 (26%), Positives = 145/338 (42%), Gaps = 32/338 (9%) Query: 150 KTQDLPPDAWQFIKDKGFLGMIIPRQYGGKQFSAYMHSQVVMKLGSRSSAAAIAVMVPNS 209 ++ P D W+ + D G LG+ + +YGG Y+ + M+ SR+SA+ +S Sbjct: 39 RSDQFPMDLWKKLGDLGVLGITVSEEYGGAGL-GYLAHIIAMEEISRASASVGLSYGAHS 97 Query: 210 LGPAELLLHYGTDAQKNHYLPRLAQGLEIPCFALTSPYAGSDAAAIPDVGIVCKGEHEGR 269 + G + QK YLPRL G I A++ P AGSD V + + + +G Sbjct: 98 NLCVNQIKRNGNEEQKRKYLPRLISGDFIGALAMSEPNAGSDV-----VSMKLRADKKGD 152 Query: 270 ETLGFRVTWSKRYITLAPIATVLGLAFRVRDPDGLLGSDAEPGITCALIPTGHPGVITGR 329 + + SK +IT P A VL + + D + G+T L+ G+ G + Sbjct: 153 R---YVLNGSKMWITNGPDADVL-VVYAKTDLEA-----GARGMTAFLVEKGYKGFSVAQ 203 Query: 330 RHWPLNAVFQNGPTEGRDVF----IPMEWVIGGAGQVGRGWRMLMECLAAGRAISLPSSS 385 + L+ + G G VF +P E V+GG VGRG +LM L R++ L Sbjct: 204 K---LDKLGMRGSHTGELVFQDCEVPEENVLGG---VGRGVNVLMSGLDFERSV-LSGGP 256 Query: 386 VGFSKLAVRGTSAYTAMRRQFKIEIGKFEGVQEALARMGGHLYMMDATRRLSALAVDAGE 445 +G + + Y R+QF IG+F+ +Q LA M + A A D + Sbjct: 257 LGIMQACMDVVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMACKAYVYAVGQACDRAD 316 Query: 446 KPSVISAISK------YHVTERGRMVVNDGMDVIGGKG 477 + A+ K + E+ + + + +GG G Sbjct: 317 SADKVRALRKDAAGAILYSAEKATWMAGEAIQSLGGNG 354 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.138 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,531,184 Number of extensions: 113850 Number of successful extensions: 392 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 386 Number of HSP's successfully gapped: 4 Length of query: 781 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 688 Effective length of database: 1,179,515 Effective search space: 811506320 Effective search space used: 811506320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 134 (56.2 bits)