BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0008.0100.p
         (337 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312916|ref|YP_003777008.1| hypothetical protein Hsero_3622...   654   0.0  
gi|300310858|ref|YP_003774950.1| magnesium and cobalt transport ...    59   8e-10

>gi|300312916|ref|YP_003777008.1| hypothetical protein Hsero_3622
           [Herbaspirillum seropedicae SmR1]
          Length = 337

 Score =  654 bits (1688), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 314/337 (93%), Positives = 326/337 (96%)

Query: 1   MHNPPDTPQALHLLYEPAGFICAFRFEDGRARQMNWNEVVDYHPSAPDFTWLHVKTADVK 60
           MHNPPDTPQALHLLYEPAGFICAFRFE+GRARQMNWN++VDY P  P FTWLHVKTADVK
Sbjct: 1   MHNPPDTPQALHLLYEPAGFICAFRFEEGRARQMNWNDLVDYRPVEPAFTWLHVKTADVK 60

Query: 61  IQQWLAHHSDIPEYITEFLLSSDTHPGLHATPEGVYGTLTDMKMEIGDTGTEKGALHFYL 120
           IQ W+ HHS IP+YITEFLLSSDTHPGLHATP+GVYGTLTDMKMEIGDTGTEKGALHFYL
Sbjct: 61  IQHWMQHHSGIPDYITEFLLSSDTHPGLHATPDGVYGTLTDMKMEIGDTGTEKGALHFYL 120

Query: 121 DRTRLLTLRTQPLISTNMLRQKVLDGGDFDNPMDLFAELLRCLADGFNARLDKLNDKVDD 180
           DRTRLLTLRTQPL+STNMLRQKVLDGGDFDNPMDLFAELLRCLADGFNARLDKLNDKVDD
Sbjct: 121 DRTRLLTLRTQPLLSTNMLRQKVLDGGDFDNPMDLFAELLRCLADGFNARLDKLNDKVDD 180

Query: 181 IEFSVLSDRRAEDRAELSGIRRQLAELRRFIAPERRILAQFQRLRPAWVPREALEDLTHA 240
           IEFSVLSDRRAEDR+ELSGIRRQLAELRR+IAPERRILAQF RLRP+WVP  ALEDLTHA
Sbjct: 181 IEFSVLSDRRAEDRSELSGIRRQLAELRRYIAPERRILAQFHRLRPSWVPLAALEDLTHA 240

Query: 241 LDELNELHSTVEAVYERAKLLQEEIASQMSEQMNRNLMALSVLTALLMPATLVSGIFGMN 300
           LDELNELHSTVEAVYERAKLLQEEIASQMSEQMNRNLMALS+LTALLMPATLVSGIFGMN
Sbjct: 241 LDELNELHSTVEAVYERAKLLQEEIASQMSEQMNRNLMALSILTALLMPATLVSGIFGMN 300

Query: 301 VAGLPGLHDETSFWIVMGLMGGLGVATVAFLKWMKIF 337
           VAGLPGLHDE SFWIVMG+MGGLGVATVAFLKWMKIF
Sbjct: 301 VAGLPGLHDEASFWIVMGVMGGLGVATVAFLKWMKIF 337


>gi|300310858|ref|YP_003774950.1| magnesium and cobalt transport
           transmembrane protein [Herbaspirillum seropedicae SmR1]
          Length = 328

 Score = 58.5 bits (140), Expect = 8e-10,   Method: Compositional matrix adjust.
 Identities = 63/329 (19%), Positives = 134/329 (40%), Gaps = 13/329 (3%)

Query: 14  LYEPAGFICAFRFEDGRA-RQMNWNEVVDYHPSAPDFTWLHVKTADVKIQQWLAHHSDIP 72
           + +P   +    ++ GR  R+++ +E+ D       F WL +   +V++   +     + 
Sbjct: 1   MIDPEMIVNCTVYKKGRKLREISLDEISDVLEEEGTFVWLGLLEPNVELMNKIKEEFCLH 60

Query: 73  EYITEFLLSSDTHPGLHATPEGVYGTLTDMKMEIGDTGTEKGALHFYLDRTRLLTLRTQP 132
           E   E   ++   P +    + ++  L   ++  G+   + G  H       ++T+R   
Sbjct: 61  ELAVEDAYAAHQRPKIEEYGDTLFVVLHTAQLTGGEV--QFGETHILTGPRFVVTVRHGA 118

Query: 133 LISTNMLRQ--KVLDGGDFDNPMDLFAELLRCLADGFNARLDKLNDKVDDIEFSVLSDRR 190
             S   +R+  ++L       P  +   ++  + D +   +D L  K    E  + +   
Sbjct: 119 SRSYAKVREHCEMLPERLVRGPSFVLYSIMDFVVDNYRPIMDDLEAKFSQYEEQLFNPGT 178

Query: 191 AEDRA---ELSGIRRQLAELRRFIAPERRILAQFQRLRPAWVPREALEDLTHALDELNEL 247
              +A   EL  ++RQL  LR   +P   +  Q  R     + ++         D +   
Sbjct: 179 ISGKAYLQELYQLKRQLIRLRSAASPLLDVCTQLLRFHEGLITKDGKAYFRDIYDHVVRF 238

Query: 248 HSTVEAVYERAKLLQEEIASQMSEQMNRNLMALSVLTALLMPATLVSGIFGMNVAGLPGL 307
             T + + E  +   +   +Q++   N  +  L+   A+L   T++  ++GMN A +PGL
Sbjct: 239 IDTGDRLREMVESAVQINLAQVTIHQNEIVQTLAGWGAILAIPTMIFSLYGMNFAHMPGL 298

Query: 308 HDETSFWIVMGLMGGLGVATVAFLKWMKI 336
             E SF +V+G     G+ +   L W ++
Sbjct: 299 SWEWSFPLVLG-----GIVSACTLLWRRL 322


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.322    0.137    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,104,946
Number of extensions: 45400
Number of successful extensions: 135
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 135
Number of HSP's successfully gapped: 3
Length of query: 337
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 251
Effective length of database: 1,212,660
Effective search space: 304377660
Effective search space used: 304377660
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 131 (55.1 bits)