BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0008.0086.p
         (531 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312905|ref|YP_003776997.1| glutamate synthase [Herbaspiril...  1030   0.0  
gi|300313212|ref|YP_003777304.1| glutamate synthase large subuni...   129   6e-31

>gi|300312905|ref|YP_003776997.1| glutamate synthase [Herbaspirillum
           seropedicae SmR1]
          Length = 531

 Score = 1030 bits (2664), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 497/531 (93%), Positives = 514/531 (96%)

Query: 1   MWANRYFVFWAVIALTLALVVLAATHGIGWGWPLIPLVLSVVGVHDLVQRRHAVLRNYPL 60
           MWANRY  FWA + LT  L+ LAA+H +GWGW LIPL L+ VG+HDL+Q+RHAVLRNYP+
Sbjct: 1   MWANRYLAFWAALVLTAILLALAASHQLGWGWGLIPLALTAVGLHDLLQKRHAVLRNYPI 60

Query: 61  IGHFRFMFEAIRPELRQYFFEDEMDGRPFSRKKRSVVYQRAKADVDSRPFGTELDMQQAG 120
           IGHFRFMFEAIRPELRQYFFEDEMDGRPFSRKKRS+VYQRAKA+VDSRPFGTELDMQQAG
Sbjct: 61  IGHFRFMFEAIRPELRQYFFEDEMDGRPFSRKKRSIVYQRAKAEVDSRPFGTELDMQQAG 120

Query: 121 HEWIGHSLSPTKIASHDFRVVVGGDRAQPYSMSVFNVSAMSFGSLSANAIRALNQGAKKG 180
           HEWIGHSLSPTKIASHDFRV+VG DRAQPYSMSVFNVSAMSFGSLSANAIRALNQGAKKG
Sbjct: 121 HEWIGHSLSPTKIASHDFRVIVGADRAQPYSMSVFNVSAMSFGSLSANAIRALNQGAKKG 180

Query: 181 NFAHDTGEGSVSEYHREFGGDLVWQIASGYFGCRNADGSFNEEKFTAQAQSPQIKMIEVK 240
           NFAHDTGEGSVSEYHREFGGDLVWQIASGYFGCRNADGSFN+EKFTAQAQSPQIKMIEVK
Sbjct: 181 NFAHDTGEGSVSEYHREFGGDLVWQIASGYFGCRNADGSFNDEKFTAQAQSPQIKMIEVK 240

Query: 241 LSQGAKPGHGGVLPAAKITPEIAATRGIPMGVDCISPAVHSSFSTPVGLLEFIEKLRGLS 300
           LSQGAKPGHGGVLPAAKITPEIAATRGIPMGVDCISPAVHSSFSTP+GLLEFIEKLRGLS
Sbjct: 241 LSQGAKPGHGGVLPAAKITPEIAATRGIPMGVDCISPAVHSSFSTPIGLLEFIEKLRGLS 300

Query: 301 GGKPVGIKLCVGHPWEFFSIAKAMLKTGIVPDFITVDGSEGGTGAAPLEFVDHVGMPLQE 360
           GGKPVGIKLCVGHPWEFF IAKAMLKT IVPDFITVDGSEGGTGAAPLEFVDHVGMPLQE
Sbjct: 301 GGKPVGIKLCVGHPWEFFGIAKAMLKTDIVPDFITVDGSEGGTGAAPLEFVDHVGMPLQE 360

Query: 361 GLVMVHNTLTGIGLRDKVRIGASGKIITAFDVARTLALGADWCNSARGFMFALGCIQSQS 420
           GLVMVHNTL GIGLRDKV+IGASGKIITAFDVARTLALGADWCNSARGFMFALGCIQSQS
Sbjct: 361 GLVMVHNTLVGIGLRDKVKIGASGKIITAFDVARTLALGADWCNSARGFMFALGCIQSQS 420

Query: 421 CHTDRCPTGVATQNAQRARALVVPDKAERVYRFHQSTLHALQELVQAAGLPHTSALRPHH 480
           CHTDRCPTGVATQNA RARALVVPDKAERV+RFHQSTLHALQELVQAAGLPHTSALRPHH
Sbjct: 421 CHTDRCPTGVATQNADRARALVVPDKAERVFRFHQSTLHALQELVQAAGLPHTSALRPHH 480

Query: 481 IVRRINDHEIQLMSNVLKYLQPNDLLEGNYRYRVYEKFWPIARAESFHPEL 531
           IVRRINDHEIQLMSNVLKYLQPNDLL+GNYRYRVYEKFWPIARAESFHPE 
Sbjct: 481 IVRRINDHEIQLMSNVLKYLQPNDLLDGNYRYRVYEKFWPIARAESFHPEF 531


>gi|300313212|ref|YP_003777304.1| glutamate synthase large subunit
            oxidoreductase [Herbaspirillum seropedicae SmR1]
          Length = 1558

 Score =  129 bits (324), Expect = 6e-31,   Method: Compositional matrix adjust.
 Identities = 102/327 (31%), Positives = 141/327 (43%), Gaps = 56/327 (17%)

Query: 145  DRAQPYSMSV--FNVSAMSFGSLSANAIRALNQGAKKGNFAHDTGEG--SVSEYHREFGG 200
            D  +P    V  F   AMS GS+S  A   L     +     +TGEG   V+ Y  E  G
Sbjct: 868  DEVEPAKEIVKRFATGAMSLGSISTEAHATLAIAMNRIGGKSNTGEGGEDVNRYRNELKG 927

Query: 201  -------------------------------DLVWQIASGYFGCRNADGSFNEEKFTAQA 229
                                             + Q+ASG FG        + E   +  
Sbjct: 928  IPIKQGATLASEIGKEHIEVDIPLLEGDSLRSRIKQVASGRFGV-------SAEYLISAD 980

Query: 230  QSPQIKMIEVKLSQGAKPGHGGVLPAAKITPEIAATRGIPMGVDCISPAVHSSFSTPVGL 289
            Q      I++K++QGAKPG GG LP  K++  IA  R    GV  ISP  H    +   L
Sbjct: 981  Q------IQIKMAQGAKPGEGGQLPGHKVSEYIAKLRVSVPGVGLISPPPHHDIYSIEDL 1034

Query: 290  LEFIEKLRGLSGGKPVGIKLC--VGHPWEFFSIAKAMLKTGIVPDFITVDGSEGGTGAAP 347
             + I  L+ ++    + +KL   VG       +AKA        D + + G +GGTGA+P
Sbjct: 1035 AQLIHDLKNVNPRASISVKLVSEVGVGTVAAGVAKAK------SDHVVIAGHDGGTGASP 1088

Query: 348  LEFVDHVGMPLQEGLVMVHNTLTGIGLRDKVRIGASGKIITAFDVARTLALGADWCNSAR 407
            L  + H G P + GL     TL   GLR+++R+ A G++ T  DV     LGAD    A 
Sbjct: 1089 LSSIKHAGSPWELGLAETQQTLVLNGLRNRIRVQADGQMKTGRDVVIGAMLGADEFGFAT 1148

Query: 408  GFMFALGCIQSQSCHTDRCPTGVATQN 434
              +   GCI  + CH + CP GVATQ+
Sbjct: 1149 APLVVEGCIMMRKCHLNTCPVGVATQD 1175


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.323    0.138    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,737,198
Number of extensions: 76335
Number of successful extensions: 223
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 222
Number of HSP's successfully gapped: 2
Length of query: 531
Length of database: 1,619,870
Length adjustment: 90
Effective length of query: 441
Effective length of database: 1,193,720
Effective search space: 526430520
Effective search space used: 526430520
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 133 (55.8 bits)