BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0085.p (734 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 1313 0.0 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 506 e-144 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 450 e-127 gi|300311453|ref|YP_003775545.1| signal transduction containing ... 409 e-115 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 391 e-109 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 1313 bits (3398), Expect = 0.0, Method: Compositional matrix adjust. Identities = 628/701 (89%), Positives = 666/701 (95%), Gaps = 2/701 (0%) Query: 36 LAMARPPRLLETLLANLDGMVYRCRDDAHWTLEFVSEGCHALTGYAPEDLLLNSRISFLQ 95 +A+ R PRLLETLL NLDG+VYRCRDDA WT+EFVSEGC ALTGY PEDLLLN R+SFL+ Sbjct: 1 MAIPRSPRLLETLLVNLDGVVYRCRDDAQWTMEFVSEGCLALTGYLPEDLLLNQRLSFLE 60 Query: 96 LTHPEDRALVRSHIDTCMRERRRIDIEYRIVHADGSLRWVWERGVGLYNAAGKVEAMEGF 155 LTHPEDR LVR+H+D CMRERRRIDIEYRIV ADG++RWVWERGVG+YNA G+VEA+EGF Sbjct: 61 LTHPEDRTLVRNHVDACMRERRRIDIEYRIVRADGAVRWVWERGVGIYNANGQVEAIEGF 120 Query: 156 LQDVTERKEAAQALQEAERRYRSIFENAIEGVFQTTPDGTYIAVNPALARIYGYHSPEDL 215 LQDVTERKEAAQALQEAERRYRSIFENAIEGVFQTTPDGTYIAVNPALARIYGYHSPEDL Sbjct: 121 LQDVTERKEAAQALQEAERRYRSIFENAIEGVFQTTPDGTYIAVNPALARIYGYHSPEDL 180 Query: 216 IVGLRDISHQLYVEPERRTEFMRLMEQHGSVSNFESRVYRRDGDIIWISENARAVYDDSG 275 IVGLRDISHQLYVEPERR EFMRLME+ GSVSNFESRVYRRDG+IIWISENARAVYD++G Sbjct: 181 IVGLRDISHQLYVEPERRQEFMRLMEEQGSVSNFESRVYRRDGEIIWISENARAVYDEAG 240 Query: 276 ALVCYEGTVEAITERKLYEAEMRHQATHDALTGLPNRNMLHEHLQRAIQVARQKGGLTAV 335 L CYEGTVEAITERK +EAEMRHQATHDALTGLPNRNMLHEHLQRA++VARQKG LTAV Sbjct: 241 KLSCYEGTVEAITERKAHEAEMRHQATHDALTGLPNRNMLHEHLQRALRVARQKGALTAV 300 Query: 336 VFVDLDQFKFINDSLGHQVGDELLKTVAQRLQACLRDTDMVARQGGDEFVLVLQNQTGGE 395 FVDLDQFKFINDSLGHQVGDELLKTVAQRLQACLR+ DMVARQGGDEFVLVLQNQT GE Sbjct: 301 AFVDLDQFKFINDSLGHQVGDELLKTVAQRLQACLREADMVARQGGDEFVLVLQNQTEGE 360 Query: 396 LGIAEVMQRILAAVARPWQAGDREFQVTASIGVSRYPVDGKDVETLLKQADSAMYRAKEQ 455 GIAEVMQRILAAVARPWQAGDREFQVTASIGVSRYP DGKDVE+LLK ADSAMYRAKEQ Sbjct: 361 AGIAEVMQRILAAVARPWQAGDREFQVTASIGVSRYPADGKDVESLLKHADSAMYRAKEQ 420 Query: 456 GRNNFQFFAPWMDTQVSNRLEMLINLRRALDQEEFKLYYQPKLSLKDGSVIGAEALIRWQ 515 GRNNFQFFAPWMD+QVSNRLEMLINLRRALDQ+EFKLYYQPKLSL+DG +IGAEALIRW+ Sbjct: 421 GRNNFQFFAPWMDSQVSNRLEMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWE 480 Query: 516 SPEQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACHQNKRWQEAGLPPIPVAVNLSPRQLN 575 SP+QGMVPPDRFIPFAEEAGLIVPIGEWVLRTAC QN+ WQ+AGLPPIPVAVNLSPRQLN Sbjct: 481 SPQQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQLN 540 Query: 576 QSLPEFVSGVLEQSGLAASCLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGY 635 QSLP+FV+GVL QSGLAAS LELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGY Sbjct: 541 QSLPDFVAGVLAQSGLAASWLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGY 600 Query: 636 SSLSYLRRFPVDALKIDKSFVRDIARDADSAAIVKAVISLAHILNLRVIAEGVEDEQQHS 695 SSLSYLRRFPVDALKIDKSFVRDI RDADSAAIVKA+ISLAHILNLRVIAEGVEDE+QH+ Sbjct: 601 SSLSYLRRFPVDALKIDKSFVRDIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHA 660 Query: 696 FLKENACDEVQGYFFGKPMAVEDFTAWMSRQAQSGQ--ACK 734 FLKENACDEVQGY+FGKPM V FTAWMS QAQ+GQ +CK Sbjct: 661 FLKENACDEVQGYYFGKPMEVAVFTAWMSEQAQAGQVLSCK 701 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 506 bits (1303), Expect = e-144, Method: Compositional matrix adjust. Identities = 278/566 (49%), Positives = 365/566 (64%), Gaps = 11/566 (1%) Query: 158 DVTERKEAAQALQEAERRYRSI--FENAIEGVFQTTPDGTYIAVNPALARIYGYHSPEDL 215 D+ R+ A +AL+ R+++I NAI + PD T VNPA ARI GY S E + Sbjct: 379 DIKARRLAERALR---LRHQAIEASANAILILSAQEPDYTIEYVNPAFARITGYSSTEAV 435 Query: 216 IVGLRDISHQLYVEPERRTEFMRLMEQHGSVSNFESRVYRRDGDIIWISENARAVYDDSG 275 +R + + T ++ + EQ N R Y +DG +W V D+SG Sbjct: 436 GKSIRFLEGRDSDPEGFETVWIAMREQREH--NVVLRNYHKDGSELWNDFYIAPVRDESG 493 Query: 276 ALVCYEGTVEAITERKLYEAEMRHQATHDALTGLPNRNMLHEHLQRAIQVARQKGGLTAV 335 + + + IT K YEAE+ QA+ D LTGL NR+ML HL++AI VA ++ V Sbjct: 494 EVTHFVAALYDITSMKRYEAELEFQASRDTLTGLINRHMLRHHLEQAIGVAERQKQALWV 553 Query: 336 VFVDLDQFKFINDSLGHQVGDELLKTVAQRLQACLRDTDMVARQGGDEFVLVLQNQTGGE 395 VFVDLD+FKF+ND+LGH+ GD LL+TVA RL+A +R+ D VAR GGDEF+++L Q E Sbjct: 554 VFVDLDRFKFVNDTLGHKAGDTLLRTVAGRLRAAVREADTVARLGGDEFIMILPEQD--E 611 Query: 396 LGI-AEVMQRILAAVARPWQAGDREFQVTASIGVSRYPVDGKDVETLLKQADSAMYRAKE 454 L + +QRI+ AVA P G +T S+GV+ YP DG D ETL+K AD AMYR K+ Sbjct: 612 LTLDTRSLQRIMDAVAAPVTLGGHTLFMTCSVGVACYPNDGTDGETLIKHADIAMYRGKQ 671 Query: 455 QGRNNFQFFAPWMDTQVSNRLEMLINLRRALDQEEFKLYYQPKLSLKDGSVIGAEALIRW 514 GRNNFQF+ P M+ Q RL + +LR ALD EF L+YQPK+ L G VIG+EALIRW Sbjct: 672 TGRNNFQFYTPAMNEQALERLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRW 731 Query: 515 QSPEQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACHQNKRWQEAGLPPIPVAVNLSPRQ- 573 Q P+ GMV P RFI AEE GLI+PIG WVLR AC Q ++WQ G I V+VNLS Q Sbjct: 732 QHPQLGMVSPARFIGLAEETGLILPIGAWVLRAACTQCRQWQLGGYADISVSVNLSVGQF 791 Query: 574 LNQSLPEFVSGVLEQSGLAASCLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGT 633 L + L ++ VLE +GL LELE+TE+++M D E ++ L LK+LG+++S+DDFGT Sbjct: 792 LQKDLVSSIADVLEDTGLPPESLELELTESLMMTDVEHAIGILAGLKKLGVRLSIDDFGT 851 Query: 634 GYSSLSYLRRFPVDALKIDKSFVRDIARDADSAAIVKAVISLAHILNLRVIAEGVEDEQQ 693 GYSSL+YL+RFP+D LKID+SFVRDI DAD AAI ++ISLA L LRVIAEGVE ++Q Sbjct: 852 GYSSLAYLKRFPIDVLKIDRSFVRDITIDADDAAITASIISLARHLKLRVIAEGVETDEQ 911 Query: 694 HSFLKENACDEVQGYFFGKPMAVEDF 719 +L+ CDE+QGY F P+ + F Sbjct: 912 LDYLRHYGCDEIQGYLFSPPVPADKF 937 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 450 bits (1158), Expect = e-127, Method: Compositional matrix adjust. Identities = 265/658 (40%), Positives = 387/658 (58%), Gaps = 35/658 (5%) Query: 78 TGYAPEDLL-LNSRISFLQLTHPEDRALVRSHIDTCMRERRRIDIEYRIVHADGSLRWVW 136 TGY E+ L ++ I + HPED +R+ + CM ++R D+E R V DGS+ W+ Sbjct: 384 TGYTVEECLAMDDFIG--TIAHPEDEPRIRAELARCMDGQKREDVELRCVRKDGSVFWLA 441 Query: 137 ERGVGLYNAAGKVEAMEGFLQDVTERKEAAQALQEAERRYRSIFENAIEGVFQTTPDGTY 196 +++A G+ +D+T+RK+ EAE R ++ ++ EG+ T + Sbjct: 442 LSCAPIHDAQGRFTGFRTSSRDITDRKQI-----EAELRISAVAFDSREGMLITDANSKI 496 Query: 197 IAVNPALARIYGYHSPEDLIVG-----LRDISHQLYVEPERRTEFMRLMEQHGSVSNFES 251 + VN A I GY + E IVG LR H E R R + G + + Sbjct: 497 LRVNKAFTEITGYSAEE--IVGKHPNLLRSDRHGPAFFQEMRESIRRFGKWQGEIWD--- 551 Query: 252 RVYRRDGDII--WISENARAVYDDSGALVCYEGTVEAITERKLYEAEMRHQATHDALTGL 309 R++G++ W++ AV + + Y T I++RKL E +R A DALT L Sbjct: 552 --RRKNGEVYPEWLT--ISAVKNKDQQVTHYVSTHHDISDRKLAEERIRELAFFDALTRL 607 Query: 310 PNRNMLHEHLQRAIQVARQKGGLTAVVFVDLDQFKFINDSLGHQVGDELLKTVAQRLQAC 369 PNR +L + L++AI ++ + A++F+DLD FK +ND++GH+ GD LLK VAQRL A Sbjct: 608 PNRTLLLDRLKQAIALSLRTKTCAALLFIDLDHFKTLNDTIGHEKGDTLLKQVAQRLLAN 667 Query: 370 LRDTDMVARQGGDEFVLVLQN----QTGGELGIAEVMQRILAAVARPWQAGDREFQVTAS 425 +R+ D VAR GGDEFV+VL+ Q + ++IL A+ +Q D E++ TAS Sbjct: 668 VRENDTVARVGGDEFVVVLEGLNELQQEAATQTRAIGEKILTALGSTYQLDDVEYRTTAS 727 Query: 426 IGVSRYPVDGKDVETLLKQADSAMYRAKEQGRNNFQFFAPWMDTQVSNRLEMLINLRRAL 485 IG + + V+ L+KQAD AMY+AKE GRN +FF P M T V R + LR+A+ Sbjct: 728 IGATVFLGRAASVDELMKQADLAMYKAKETGRNALRFFDPAMQTAVLERATLEAGLRKAI 787 Query: 486 DQEEFKLYYQPKLSLKDGSVIGAEALIRWQSPEQGMVPPDRFIPFAEEAGLIVPIGEWVL 545 D + L+YQP++ ++ G V GAE L+RWQ P++GMVPP FIP AEE GLI+ +G WVL Sbjct: 788 DGNQLLLHYQPQV-VEGGRVTGAEVLVRWQHPQRGMVPPGDFIPLAEETGLILSLGNWVL 846 Query: 546 RTACHQNKRWQ-EAGLPPIPVAVNLSPRQLNQSLPEFVSGVL---EQSGLAASCLELEIT 601 TAC Q W + + + +AVN+S QL + P+FV VL E++G L+LE+T Sbjct: 847 ETACRQLATWAAQPQMADLSIAVNVSAPQLRE--PDFVQTVLDTIERTGANPRRLKLELT 904 Query: 602 ENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSLSYLRRFPVDALKIDKSFVRDIAR 661 E++++ + E + + ALK G++ S+DDFGTGYSSLSYL+R P++ LKID+SFVRD+ Sbjct: 905 ESLLVDNVEDIIQKMFALKAYGVEFSLDDFGTGYSSLSYLKRLPLNQLKIDQSFVRDVLI 964 Query: 662 DADSAAIVKAVISLAHILNLRVIAEGVEDEQQHSFLKENACDEVQGYFFGKPMAVEDF 719 D + A+I K +++LA L L VIAEGVE + Q SFL + C QGYFF +P+ VE F Sbjct: 965 DPNDASIAKTIVNLAQSLGLGVIAEGVETDAQRSFLADAGCHAYQGYFFCRPVTVEAF 1022 >gi|300311453|ref|YP_003775545.1| signal transduction containing PAS/GGDEF/EAL domains protein [Herbaspirillum seropedicae SmR1] Length = 683 Score = 409 bits (1052), Expect = e-115, Method: Compositional matrix adjust. Identities = 234/574 (40%), Positives = 338/574 (58%), Gaps = 20/574 (3%) Query: 158 DVTERKEAAQALQEAERRYRSIFENAIEGVFQTTPDGTYIAVNPALARIYGYHSPEDLIV 217 D +R A ++ + I E V P G + N +++GY + E + Sbjct: 106 DTEQRVRALDQIEAKLEQQAQILNQMHESVITMDPAGFITSWNRGAEQLFGYSAME--AI 163 Query: 218 GLRDISHQLYVEPERRTEFMRLMEQHGSVSNFESRVYRRDGDIIWISENARAVYDDSGAL 277 G R++ LY + L +HG E R ++ G++ W S + + D +G Sbjct: 164 G-RNVLF-LYENEDEEDSLHDLFLEHGG-REMEVRRRKKSGEVFWASLSLSVMRDQNGHP 220 Query: 278 VCYEGTVEAITERKLYEAEMRHQATHDALTGLPNRNMLHEHLQRAIQVARQKGGLTAVVF 337 + G + ITERK E + H A +DALTGLPNR +L + +A++ AR + V+F Sbjct: 221 IGLIGYLNDITERKNAEKLIHHLAYYDALTGLPNRTLLTRTVDQALEEARHENLHGCVMF 280 Query: 338 VDLDQFKFINDSLGHQVGDELLKTVAQRLQACLRDTDMVARQGGDEFVLVLQNQTGGELG 397 +DL++FK IND+LGH GD LL VA RL+ LR+ D+VAR G DEF + L G Sbjct: 281 IDLNRFKPINDTLGHVAGDMLLVEVAMRLRRALREQDVVARLGADEFAVAL-------FG 333 Query: 398 I-----AEVMQRILAAVARPWQAGDREFQVTASIGVSRYPVDGKDVETLLKQADSAMYRA 452 I A V Q++LA P+ E +V ASIGVS YP + D ETLL+ AD AMYRA Sbjct: 334 IDKDSPAYVAQKLLALFDDPFFIDGHELRVGASIGVSLYPENATDTETLLRLADIAMYRA 393 Query: 453 KEQGRNNFQFFAPWMDTQVSNRLEML---INLRRALDQEEFKLYYQPKLSLKDGSVIGAE 509 K+ G NN +A + + N L++L L A + E LYYQPK+ ++ ++ GAE Sbjct: 394 KQGGENNEGGYACYSEEMNRNTLDLLRIETGLLHAFEYNELLLYYQPKVDMQTLAITGAE 453 Query: 510 ALIRWQSPEQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACHQNKRWQEAGLPPIPVAVNL 569 AL+RWQ PE+G++ P FIP AEE GLIV + +WVL C Q + W++AGLPPI +A+N+ Sbjct: 454 ALVRWQHPERGLLLPGEFIPIAEETGLIVQLSDWVLDAVCRQARAWKDAGLPPIRIALNV 513 Query: 570 SPRQLNQSLPEFVSGVLEQSGLAASCLELEITENVVMKDAEKSVATLHALKRLGLQISVD 629 + R+ +SLP+ V LE+ L+ LELEITE+++M ++ ++ + + LG+ +S+D Sbjct: 514 TAREFTRSLPDRVGAALERHMLSGEWLELEITESMLMHSTDRVISIMEKICGLGITVSLD 573 Query: 630 DFGTGYSSLSYLRRFPVDALKIDKSFVRDIARDADSAAIVKAVISLAHILNLRVIAEGVE 689 DFGTGYSSLSYL+RFP++ LKID+SF I D + AI A+I +A L +VIAEGVE Sbjct: 574 DFGTGYSSLSYLKRFPINTLKIDRSFTMGIPDDTNDCAIASAIIGIAKQLRHKVIAEGVE 633 Query: 690 DEQQHSFLKENACDEVQGYFFGKPMAVEDFTAWM 723 DE+Q +FL+E C+E+QGY F +P+ DF A + Sbjct: 634 DEKQFNFLREAGCNELQGYIFSRPVPAPDFHAML 667 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 391 bits (1004), Expect = e-109, Method: Compositional matrix adjust. Identities = 205/436 (47%), Positives = 278/436 (63%), Gaps = 5/436 (1%) Query: 296 EMRHQATHDALTGLPNRNMLHEHLQRAIQVARQKGGLTAVVFVDLDQFKFINDSLGHQVG 355 E+ HD LT LPNR +L + L +AI A ++ G A++F+DLD FK INDSLGH VG Sbjct: 261 ELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFALMFMDLDGFKAINDSLGHHVG 320 Query: 356 DELLKTVAQRLQACLRDTDMVARQGGDEFVLVLQ-NQTGGELGIAEVMQRILAAVARPWQ 414 D LL VAQRL + R D VAR GGDEFV+++ N +G+AE +++ AV +P++ Sbjct: 321 DRLLVEVAQRLTSSSRANDTVARLGGDEFVILVDLNTPEDAMGVAE---KLVEAVNQPFK 377 Query: 415 AGDREFQVTASIGVSRYPVDGKDVETLLKQADSAMYRAKEQGRNNFQFFAPWMDTQVSNR 474 E +V+AS+G++ YP DG L+ AD+AMY K GRN + FF P M+ + Sbjct: 378 VDQHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGRNGYHFFEPSMNANAQQQ 437 Query: 475 LEMLINLRRALDQEEFKLYYQPKLSLKDGSVIGAEALIRWQSPEQGMVPPDRFIPFAEEA 534 L+ L +LR AL++ +F+L YQPK SL G V+GAEAL+RW+ P G+V PD+FI AE + Sbjct: 438 LQWLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHGLVAPDQFIGLAERS 497 Query: 535 GLIVPIGEWVLRTACHQNKRWQEAGLPPIPVAVNLSPRQL-NQSLPEFVSGVLEQSGLAA 593 GLI+PIG WVL AC Q + W G ++VNLS Q + L E V LE+ L Sbjct: 498 GLILPIGAWVLDEACRQMREWFALGYVDWTMSVNLSALQFADPHLLEVVRTTLERHQLPP 557 Query: 594 SCLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSLSYLRRFPVDALKIDK 653 CL LE+TE+ M D E S+ TL + LG++IS+DDFGTGYSSL YL+R P + LKID+ Sbjct: 558 PCLTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYSSLLYLKRLPANELKIDR 617 Query: 654 SFVRDIARDADSAAIVKAVISLAHILNLRVIAEGVEDEQQHSFLKENACDEVQGYFFGKP 713 FVR++ +D + AAIV A+++L LNLR++AEGVE +Q FL CD +QGY G+P Sbjct: 618 GFVRELRQDGEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDFLTAAGCDALQGYLMGRP 677 Query: 714 MAVEDFTAWMSRQAQS 729 M E F M + +S Sbjct: 678 MTPELFLQTMRERHKS 693 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,129,256 Number of extensions: 86248 Number of successful extensions: 413 Number of sequences better than 1.0e-08: 47 Number of HSP's gapped: 325 Number of HSP's successfully gapped: 47 Length of query: 734 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 642 Effective length of database: 1,184,250 Effective search space: 760288500 Effective search space used: 760288500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)