BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0046.p (186 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312875|ref|YP_003776967.1| glucokinase [Herbaspirillum ser... 293 8e-81 >gi|300312875|ref|YP_003776967.1| glucokinase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 293 bits (749), Expect = 8e-81, Method: Compositional matrix adjust. Identities = 145/162 (89%), Positives = 153/162 (94%) Query: 21 SEGGHVAFAPANAREAAVLAYAWQFHEHVSAERMVSGPGLEFIHRALLAIEGHPAAELKA 80 SEGGH AFAPA+ REAAVLAYAWQFHEHVSAER+VSGPGLE IHRALLAI+GHPAAEL A Sbjct: 168 SEGGHAAFAPADPREAAVLAYAWQFHEHVSAERLVSGPGLELIHRALLAIDGHPAAELSA 227 Query: 81 AEIVDGALKQGDAVCLETLDLFCSMLGTVAADLALTLGALGGIYIGGGVVPHLGEYFARS 140 A+IV+GA + GDA+C ETL LFCSMLGTVAADLALTLGALGGIYIG GVVPHLG+YFARS Sbjct: 228 AQIVEGARQHGDALCQETLALFCSMLGTVAADLALTLGALGGIYIGVGVVPHLGDYFARS 287 Query: 141 PFRARFENKGRMSVLTKAIPTYVITAEYPAFTGVSAILADRL 182 PFRARFENKGRMSVLTKAIPTYVITAEYPAFTGVSAILADRL Sbjct: 288 PFRARFENKGRMSVLTKAIPTYVITAEYPAFTGVSAILADRL 329 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 535,040 Number of extensions: 18482 Number of successful extensions: 62 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 62 Number of HSP's successfully gapped: 1 Length of query: 186 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 106 Effective length of database: 1,241,070 Effective search space: 131553420 Effective search space used: 131553420 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)